PhytophthoraV1, Main, Exploration, bibRecord, 000701

Phytophthora infestans RXLR effector SFI5 requires association with calmodulin for PTI/MTI suppressing activity.

Identifieur interne : 000701 ( Main/Exploration ); précédent : 000700; suivant : 000702

Phytophthora infestans RXLR effector SFI5 requires association with calmodulin for PTI/MTI suppressing activity.

Auteurs : Xiangzi Zheng [Allemagne, République populaire de Chine] ; Nadine Wagener [Allemagne] ; Hazel Mclellan [Royaume-Uni] ; Petra C. Boevink [Royaume-Uni] ; Chenlei Hua [Allemagne] ; Paul R J. Birch [Royaume-Uni] ; Frédéric Brunner [Allemagne, Espagne]

Source :

The New phytologist [ 1469-8137 ] ; 2018.

RBID : pubmed:29932222

Descripteurs français

KwdFr :
- Calcium (pharmacologie), Calmoduline (métabolisme), Immunité des plantes (effets des médicaments et des substances chimiques), Liaison aux protéines (effets des médicaments et des substances chimiques), Lycopersicon esculentum (immunologie), Lycopersicon esculentum (microbiologie), Lycopersicon esculentum (métabolisme), Membrane cellulaire (métabolisme), Molécules contenant des motifs associés aux pathogènes (métabolisme), Motifs d'acides aminés (MeSH), Peptides (composition chimique), Peptides (métabolisme), Phytophthora infestans (effets des médicaments et des substances chimiques), Phytophthora infestans (métabolisme), Protéines (composition chimique), Protéines (métabolisme), Séquence d'acides aminés (MeSH).
MESH :
- composition chimique : Peptides, Protéines.
- effets des médicaments et des substances chimiques : Immunité des plantes, Liaison aux protéines, Phytophthora infestans.
- immunologie : Lycopersicon esculentum.
- microbiologie : Lycopersicon esculentum.
- métabolisme : Calmoduline, Lycopersicon esculentum, Membrane cellulaire, Molécules contenant des motifs associés aux pathogènes, Peptides, Phytophthora infestans, Protéines.
- pharmacologie : Calcium.
- Motifs d'acides aminés, Séquence d'acides aminés.

English descriptors

KwdEn :
- Amino Acid Motifs (MeSH), Amino Acid Sequence (MeSH), Calcium (pharmacology), Calmodulin (metabolism), Cell Membrane (metabolism), Lycopersicon esculentum (immunology), Lycopersicon esculentum (metabolism), Lycopersicon esculentum (microbiology), Pathogen-Associated Molecular Pattern Molecules (metabolism), Peptides (chemistry), Peptides (metabolism), Phytophthora infestans (drug effects), Phytophthora infestans (metabolism), Plant Immunity (drug effects), Protein Binding (drug effects), Proteins (chemistry), Proteins (metabolism).
MESH :
- chemical , chemistry : Peptides, Proteins.
- chemical , metabolism : Calmodulin, Pathogen-Associated Molecular Pattern Molecules, Peptides, Proteins.
- chemical , pharmacology : Calcium.
- drug effects : Phytophthora infestans, Plant Immunity, Protein Binding.
- immunology : Lycopersicon esculentum.
- metabolism : Cell Membrane, Lycopersicon esculentum, Phytophthora infestans.
- microbiology : Lycopersicon esculentum.
- Amino Acid Motifs, Amino Acid Sequence.

Abstract

Pathogens secrete effector proteins to interfere with plant innate immunity, in which Ca²⁺ /calmodulin (CaM) signalling plays key roles. Thus far, few effectors have been identified that directly interact with CaM for defence suppression. Here, we report that SFI5, an RXLR effector from Phytophthora infestans, suppresses microbe-associated molecular pattern (MAMP)-triggered immunity (MTI) by interacting with host CaMs. We predicted the CaM-binding site in SFI5 using in silico analysis. The interaction between SFI5 and CaM was tested by both in vitro and in vivo assays. MTI suppression by SFI5 and truncated variants were performed in a tomato protoplast system. We found that both the predicted CaM-binding site and the full-length SFI5 protein interact with CaM in the presence of Ca²⁺ . MTI responses, such as FRK1 upregulation, reactive oxygen species accumulation, and mitogen-activated protein kinase activation were suppressed by truncated SFI5 proteins containing the C-terminal CaM-binding site but not by those without it. The plasma membrane localization of SFI5 and its ability to enhance infection were also perturbed by loss of the CaM-binding site. We conclude that CaM-binding is required for localization and activity of SFI5. We propose that SFI5 suppresses plant immunity by interfering with immune signalling components after activation by CaMs.

DOI: 10.1111/nph.15250
PubMed: 29932222
PubMed Central: PMC6099356

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000735
to stream Main, to step Curation: 000735

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Phytophthora infestans RXLR effector SFI5 requires association with calmodulin for PTI/MTI suppressing activity.</title>
<author><name sortKey="Zheng, Xiangzi" sort="Zheng, Xiangzi" uniqKey="Zheng X" first="Xiangzi" last="Zheng">Xiangzi Zheng</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Tübingen</region>
<settlement type="city">Tübingen</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Center for Molecular Cell and Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Center for Molecular Cell and Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002</wicri:regionArea>
<wicri:noRegion>350002</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wagener, Nadine" sort="Wagener, Nadine" uniqKey="Wagener N" first="Nadine" last="Wagener">Nadine Wagener</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Tübingen</region>
<settlement type="city">Tübingen</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mclellan, Hazel" sort="Mclellan, Hazel" uniqKey="Mclellan H" first="Hazel" last="Mclellan">Hazel Mclellan</name>
<affiliation wicri:level="1"><nlm:affiliation>Division of Plant Sciences, University of Dundee (at James Hutton Institute), Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Division of Plant Sciences, University of Dundee (at James Hutton Institute), Errol Rd, Invergowrie, Dundee, DD2 5DA</wicri:regionArea>
<wicri:noRegion>DD2 5DA</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Boevink, Petra C" sort="Boevink, Petra C" uniqKey="Boevink P" first="Petra C" last="Boevink">Petra C. Boevink</name>
<affiliation wicri:level="1"><nlm:affiliation>Cell and Molecular Sciences, The James Hutton Institute, Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Cell and Molecular Sciences, The James Hutton Institute, Errol Rd, Invergowrie, Dundee, DD2 5DA</wicri:regionArea>
<wicri:noRegion>DD2 5DA</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hua, Chenlei" sort="Hua, Chenlei" uniqKey="Hua C" first="Chenlei" last="Hua">Chenlei Hua</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Tübingen</region>
<settlement type="city">Tübingen</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Birch, Paul R J" sort="Birch, Paul R J" uniqKey="Birch P" first="Paul R J" last="Birch">Paul R J. Birch</name>
<affiliation wicri:level="1"><nlm:affiliation>Division of Plant Sciences, University of Dundee (at James Hutton Institute), Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Division of Plant Sciences, University of Dundee (at James Hutton Institute), Errol Rd, Invergowrie, Dundee, DD2 5DA</wicri:regionArea>
<wicri:noRegion>DD2 5DA</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Cell and Molecular Sciences, The James Hutton Institute, Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Cell and Molecular Sciences, The James Hutton Institute, Errol Rd, Invergowrie, Dundee, DD2 5DA</wicri:regionArea>
<wicri:noRegion>DD2 5DA</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Brunner, Frederic" sort="Brunner, Frederic" uniqKey="Brunner F" first="Frédéric" last="Brunner">Frédéric Brunner</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Tübingen</region>
<settlement type="city">Tübingen</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>PlantResponse Biotech, S.L., Centre for Plant Biotechnology and Genomics (CBGP), Campus de Montegancedo, 28223, Pozuelo de Alarcón, Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>PlantResponse Biotech, S.L., Centre for Plant Biotechnology and Genomics (CBGP), Campus de Montegancedo, 28223, Pozuelo de Alarcón, Madrid</wicri:regionArea>
<placeName><settlement type="city">Madrid</settlement>
<region nuts="2" type="region">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2018">2018</date>
<idno type="RBID">pubmed:29932222</idno>
<idno type="pmid">29932222</idno>
<idno type="doi">10.1111/nph.15250</idno>
<idno type="pmc">PMC6099356</idno>
<idno type="wicri:Area/Main/Corpus">000735</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000735</idno>
<idno type="wicri:Area/Main/Curation">000735</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000735</idno>
<idno type="wicri:Area/Main/Exploration">000735</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Phytophthora infestans RXLR effector SFI5 requires association with calmodulin for PTI/MTI suppressing activity.</title>
<author><name sortKey="Zheng, Xiangzi" sort="Zheng, Xiangzi" uniqKey="Zheng X" first="Xiangzi" last="Zheng">Xiangzi Zheng</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Tübingen</region>
<settlement type="city">Tübingen</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Center for Molecular Cell and Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Center for Molecular Cell and Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002</wicri:regionArea>
<wicri:noRegion>350002</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wagener, Nadine" sort="Wagener, Nadine" uniqKey="Wagener N" first="Nadine" last="Wagener">Nadine Wagener</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Tübingen</region>
<settlement type="city">Tübingen</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mclellan, Hazel" sort="Mclellan, Hazel" uniqKey="Mclellan H" first="Hazel" last="Mclellan">Hazel Mclellan</name>
<affiliation wicri:level="1"><nlm:affiliation>Division of Plant Sciences, University of Dundee (at James Hutton Institute), Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Division of Plant Sciences, University of Dundee (at James Hutton Institute), Errol Rd, Invergowrie, Dundee, DD2 5DA</wicri:regionArea>
<wicri:noRegion>DD2 5DA</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Boevink, Petra C" sort="Boevink, Petra C" uniqKey="Boevink P" first="Petra C" last="Boevink">Petra C. Boevink</name>
<affiliation wicri:level="1"><nlm:affiliation>Cell and Molecular Sciences, The James Hutton Institute, Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Cell and Molecular Sciences, The James Hutton Institute, Errol Rd, Invergowrie, Dundee, DD2 5DA</wicri:regionArea>
<wicri:noRegion>DD2 5DA</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hua, Chenlei" sort="Hua, Chenlei" uniqKey="Hua C" first="Chenlei" last="Hua">Chenlei Hua</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Tübingen</region>
<settlement type="city">Tübingen</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Birch, Paul R J" sort="Birch, Paul R J" uniqKey="Birch P" first="Paul R J" last="Birch">Paul R J. Birch</name>
<affiliation wicri:level="1"><nlm:affiliation>Division of Plant Sciences, University of Dundee (at James Hutton Institute), Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Division of Plant Sciences, University of Dundee (at James Hutton Institute), Errol Rd, Invergowrie, Dundee, DD2 5DA</wicri:regionArea>
<wicri:noRegion>DD2 5DA</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Cell and Molecular Sciences, The James Hutton Institute, Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Cell and Molecular Sciences, The James Hutton Institute, Errol Rd, Invergowrie, Dundee, DD2 5DA</wicri:regionArea>
<wicri:noRegion>DD2 5DA</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Brunner, Frederic" sort="Brunner, Frederic" uniqKey="Brunner F" first="Frédéric" last="Brunner">Frédéric Brunner</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Tübingen</region>
<settlement type="city">Tübingen</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>PlantResponse Biotech, S.L., Centre for Plant Biotechnology and Genomics (CBGP), Campus de Montegancedo, 28223, Pozuelo de Alarcón, Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>PlantResponse Biotech, S.L., Centre for Plant Biotechnology and Genomics (CBGP), Campus de Montegancedo, 28223, Pozuelo de Alarcón, Madrid</wicri:regionArea>
<placeName><settlement type="city">Madrid</settlement>
<region nuts="2" type="region">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">The New phytologist</title>
<idno type="eISSN">1469-8137</idno>
<imprint><date when="2018" type="published">2018</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs (MeSH)</term>
<term>Amino Acid Sequence (MeSH)</term>
<term>Calcium (pharmacology)</term>
<term>Calmodulin (metabolism)</term>
<term>Cell Membrane (metabolism)</term>
<term>Lycopersicon esculentum (immunology)</term>
<term>Lycopersicon esculentum (metabolism)</term>
<term>Lycopersicon esculentum (microbiology)</term>
<term>Pathogen-Associated Molecular Pattern Molecules (metabolism)</term>
<term>Peptides (chemistry)</term>
<term>Peptides (metabolism)</term>
<term>Phytophthora infestans (drug effects)</term>
<term>Phytophthora infestans (metabolism)</term>
<term>Plant Immunity (drug effects)</term>
<term>Protein Binding (drug effects)</term>
<term>Proteins (chemistry)</term>
<term>Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Calcium (pharmacologie)</term>
<term>Calmoduline (métabolisme)</term>
<term>Immunité des plantes (effets des médicaments et des substances chimiques)</term>
<term>Liaison aux protéines (effets des médicaments et des substances chimiques)</term>
<term>Lycopersicon esculentum (immunologie)</term>
<term>Lycopersicon esculentum (microbiologie)</term>
<term>Lycopersicon esculentum (métabolisme)</term>
<term>Membrane cellulaire (métabolisme)</term>
<term>Molécules contenant des motifs associés aux pathogènes (métabolisme)</term>
<term>Motifs d'acides aminés (MeSH)</term>
<term>Peptides (composition chimique)</term>
<term>Peptides (métabolisme)</term>
<term>Phytophthora infestans (effets des médicaments et des substances chimiques)</term>
<term>Phytophthora infestans (métabolisme)</term>
<term>Protéines (composition chimique)</term>
<term>Protéines (métabolisme)</term>
<term>Séquence d'acides aminés (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Peptides</term>
<term>Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calmodulin</term>
<term>Pathogen-Associated Molecular Pattern Molecules</term>
<term>Peptides</term>
<term>Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Calcium</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Peptides</term>
<term>Protéines</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Phytophthora infestans</term>
<term>Plant Immunity</term>
<term>Protein Binding</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Immunité des plantes</term>
<term>Liaison aux protéines</term>
<term>Phytophthora infestans</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lycopersicon esculentum</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Lycopersicon esculentum</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term>
<term>Lycopersicon esculentum</term>
<term>Phytophthora infestans</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lycopersicon esculentum</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lycopersicon esculentum</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Calmoduline</term>
<term>Lycopersicon esculentum</term>
<term>Membrane cellulaire</term>
<term>Molécules contenant des motifs associés aux pathogènes</term>
<term>Peptides</term>
<term>Phytophthora infestans</term>
<term>Protéines</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Calcium</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Motifs</term>
<term>Amino Acid Sequence</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Motifs d'acides aminés</term>
<term>Séquence d'acides aminés</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Pathogens secrete effector proteins to interfere with plant innate immunity, in which Ca<sup>2+</sup>
 /calmodulin (CaM) signalling plays key roles. Thus far, few effectors have been identified that directly interact with CaM for defence suppression. Here, we report that SFI5, an RXLR effector from Phytophthora infestans, suppresses microbe-associated molecular pattern (MAMP)-triggered immunity (MTI) by interacting with host CaMs. We predicted the CaM-binding site in SFI5 using in silico analysis. The interaction between SFI5 and CaM was tested by both in vitro and in vivo assays. MTI suppression by SFI5 and truncated variants were performed in a tomato protoplast system. We found that both the predicted CaM-binding site and the full-length SFI5 protein interact with CaM in the presence of Ca<sup>2+</sup>
 . MTI responses, such as FRK1 upregulation, reactive oxygen species accumulation, and mitogen-activated protein kinase activation were suppressed by truncated SFI5 proteins containing the C-terminal CaM-binding site but not by those without it. The plasma membrane localization of SFI5 and its ability to enhance infection were also perturbed by loss of the CaM-binding site. We conclude that CaM-binding is required for localization and activity of SFI5. We propose that SFI5 suppresses plant immunity by interfering with immune signalling components after activation by CaMs.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29932222</PMID>
<DateCompleted><Year>2019</Year>
<Month>09</Month>
<Day>25</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>09</Month>
<Day>30</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>219</Volume>
<Issue>4</Issue>
<PubDate><Year>2018</Year>
<Month>09</Month>
</PubDate>
</JournalIssue>
<Title>The New phytologist</Title>
<ISOAbbreviation>New Phytol</ISOAbbreviation>
</Journal>
<ArticleTitle>Phytophthora infestans RXLR effector SFI5 requires association with calmodulin for PTI/MTI suppressing activity.</ArticleTitle>
<Pagination><MedlinePgn>1433-1446</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1111/nph.15250</ELocationID>
<Abstract><AbstractText>Pathogens secrete effector proteins to interfere with plant innate immunity, in which Ca<sup>2+</sup>
 /calmodulin (CaM) signalling plays key roles. Thus far, few effectors have been identified that directly interact with CaM for defence suppression. Here, we report that SFI5, an RXLR effector from Phytophthora infestans, suppresses microbe-associated molecular pattern (MAMP)-triggered immunity (MTI) by interacting with host CaMs. We predicted the CaM-binding site in SFI5 using in silico analysis. The interaction between SFI5 and CaM was tested by both in vitro and in vivo assays. MTI suppression by SFI5 and truncated variants were performed in a tomato protoplast system. We found that both the predicted CaM-binding site and the full-length SFI5 protein interact with CaM in the presence of Ca<sup>2+</sup>
 . MTI responses, such as FRK1 upregulation, reactive oxygen species accumulation, and mitogen-activated protein kinase activation were suppressed by truncated SFI5 proteins containing the C-terminal CaM-binding site but not by those without it. The plasma membrane localization of SFI5 and its ability to enhance infection were also perturbed by loss of the CaM-binding site. We conclude that CaM-binding is required for localization and activity of SFI5. We propose that SFI5 suppresses plant immunity by interfering with immune signalling components after activation by CaMs.</AbstractText>
<CopyrightInformation>© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zheng</LastName>
<ForeName>Xiangzi</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Center for Molecular Cell and Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wagener</LastName>
<ForeName>Nadine</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>McLellan</LastName>
<ForeName>Hazel</ForeName>
<Initials>H</Initials>
<Identifier Source="ORCID">0000-0003-4490-7053</Identifier>
<AffiliationInfo><Affiliation>Division of Plant Sciences, University of Dundee (at James Hutton Institute), Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Boevink</LastName>
<ForeName>Petra C</ForeName>
<Initials>PC</Initials>
<Identifier Source="ORCID">0000-0002-7021-9097</Identifier>
<AffiliationInfo><Affiliation>Cell and Molecular Sciences, The James Hutton Institute, Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hua</LastName>
<ForeName>Chenlei</ForeName>
<Initials>C</Initials>
<Identifier Source="ORCID">0000-0002-6630-9049</Identifier>
<AffiliationInfo><Affiliation>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Birch</LastName>
<ForeName>Paul R J</ForeName>
<Initials>PRJ</Initials>
<Identifier Source="ORCID">0000-0002-6559-3746</Identifier>
<AffiliationInfo><Affiliation>Division of Plant Sciences, University of Dundee (at James Hutton Institute), Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Cell and Molecular Sciences, The James Hutton Institute, Errol Rd, Invergowrie, Dundee, DD2 5DA, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Brunner</LastName>
<ForeName>Frédéric</ForeName>
<Initials>F</Initials>
<Identifier Source="ORCID">0000-0001-6004-365X</Identifier>
<AffiliationInfo><Affiliation>Department of Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>PlantResponse Biotech, S.L., Centre for Plant Biotechnology and Genomics (CBGP), Campus de Montegancedo, 28223, Pozuelo de Alarcón, Madrid, Spain.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>BB/G015244/1</GrantID>
<Agency>Biotechnology and Biological Sciences Research Council</Agency>
<Country>United Kingdom</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2018</Year>
<Month>06</Month>
<Day>22</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>New Phytol</MedlineTA>
<NlmUniqueID>9882884</NlmUniqueID>
<ISSNLinking>0028-646X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D002147">Calmodulin</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000069452">Pathogen-Associated Molecular Pattern Molecules</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010455">Peptides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011506">Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>SY7Q814VUP</RegistryNumber>
<NameOfSubstance UI="D002118">Calcium</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D020816" MajorTopicYN="N">Amino Acid Motifs</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002118" MajorTopicYN="N">Calcium</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002147" MajorTopicYN="N">Calmodulin</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000069452" MajorTopicYN="N">Pathogen-Associated Molecular Pattern Molecules</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010455" MajorTopicYN="N">Peptides</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057865" MajorTopicYN="Y">Plant Immunity</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011506" MajorTopicYN="N">Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">MAMP-triggered immunity (MTI)</Keyword>
<Keyword MajorTopicYN="Y">RXLR effector</Keyword>
<Keyword MajorTopicYN="Y">SFI5</Keyword>
<Keyword MajorTopicYN="Y">calcium</Keyword>
<Keyword MajorTopicYN="Y">calmodulin</Keyword>
<Keyword MajorTopicYN="Y">tomato protoplast</Keyword>
<Keyword MajorTopicYN="Y">virulence</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year>
<Month>03</Month>
<Day>29</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2018</Year>
<Month>05</Month>
<Day>03</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2018</Year>
<Month>6</Month>
<Day>23</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2019</Year>
<Month>9</Month>
<Day>26</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2018</Year>
<Month>6</Month>
<Day>23</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">29932222</ArticleId>
<ArticleId IdType="doi">10.1111/nph.15250</ArticleId>
<ArticleId IdType="pmc">PMC6099356</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Cell Host Microbe. 2016 Jan 13;19(1):67-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26764598</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2014;1127:213-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24643564</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEBS Lett. 2008 Mar 19;582(6):943-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18298954</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 1977 Oct;82(2):580-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">71866</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2008 Jul;20(7):1930-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18621946</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2006 Sep 1;313(5791):1261-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16946064</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2014 Mar;26(3):1345-59</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24632534</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Plant Biol. 2013 Apr 27;13:70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23621884</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2016 Jan 29;7:10311</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26822079</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2009 Sep 17;461(7262):393-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19741609</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Biol. 2014 Jan 20;24(2):134-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24388849</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2005 May;42(3):444-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15842628</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Plant Sci. 2005 Aug;10(8):383-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16023399</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2016 Jan;209(1):294-306</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26315018</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2014 Apr 24;10(4):e1004057</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24763622</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1992 Aug 28;257(5074):1251-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1519061</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Biol. 2013 Dec;11(12):e1001732</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24339748</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Microbiol. 2014 Jul 22;5:320</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25101059</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Biophys Biomol Struct. 1995;24:85-116</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7663132</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2009 Feb 26;457(7233):1154-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19122675</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2007 Nov 1;450(7166):115-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17914356</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2003 Jul 1;31(13):3497-500</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12824352</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2005 Feb 22;102(8):3135-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15703292</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Immunol. 2005 Oct;6(10):973-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16177805</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Plant Biol. 2004;55:373-99</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15377225</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Plant Biol. 2005;56:435-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15862103</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Microbiol. 2011 Feb;14(1):39-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21227738</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2016 Jan;209(2):664-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26315131</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2011 Nov;7(11):e1002353</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22102810</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2002 Mar 28;416(6879):447-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11919636</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2016 May;171(1):645-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26966171</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Biochem Sci. 1990 Feb;15(2):59-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2186516</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunol Rev. 2004 Apr;198:249-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15199967</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2013;9(10):e1003670</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24130484</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2002 Sep 23;3(10):RESEARCH0056</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12372144</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2015 Aug;207(3):735-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25760731</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2010 May 25;107(21):9909-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20457921</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12492-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23836668</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2000 Dec;12(12):2323-2338</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11148281</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Phytopathol. 2013;51:473-98</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23725467</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2009 Apr;149(4):1773-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19211698</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Electrophoresis. 2006 Oct;27(20):3949-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16991206</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2002 Aug;5(4):318-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12179965</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2011 Jun;23(6):2010-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21642548</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1997 Oct 31;91(3):295-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9363937</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2006 Oct;48(2):165-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16965554</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Biochim Biophys Sin (Shanghai). 2011 Nov;43(11):867-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21908855</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4874-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18344324</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2014 Apr 24;54(2):263-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24766890</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2015 Aug 28;11(8):e1005139</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26317500</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Biol. 2017 Apr 3;27(7):981-991</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28318979</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Host Microbe. 2012 Oct 18;12(4):484-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23084917</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Microbiol. 2013 Nov;11(11):800-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24129511</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2006;171(2):249-69</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16866934</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 2013 Jul;1833(7):1766-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23380707</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Microbiol. 2016 Dec;34:127-135</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27723513</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2014 Dec;204(4):803-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25539003</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2011 May;190(3):653-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21348873</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2009 May 8;324(5928):742-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19423812</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Physiol. 2014 Jul;144(1):105-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24935744</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Plant Biol. 2009;60:379-406</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19400727</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2012;869:49-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22585476</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2012 Aug;15(4):483-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22483402</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Plant Sci. 2015 Oct 29;6:940</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26579181</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2006 May 5;125(3):563-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16678099</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant. 2015 Apr;8(4):521-39</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25744358</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Struct Funct Genomics. 2000;1(1):8-14</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12836676</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Allemagne</li>
<li>Espagne</li>
<li>Royaume-Uni</li>
<li>République populaire de Chine</li>
</country>
<region><li>Bade-Wurtemberg</li>
<li>Communauté de Madrid</li>
<li>District de Tübingen</li>
</region>
<settlement><li>Madrid</li>
<li>Tübingen</li>
</settlement>
</list>
<tree><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Zheng, Xiangzi" sort="Zheng, Xiangzi" uniqKey="Zheng X" first="Xiangzi" last="Zheng">Xiangzi Zheng</name>
</region>
<name sortKey="Brunner, Frederic" sort="Brunner, Frederic" uniqKey="Brunner F" first="Frédéric" last="Brunner">Frédéric Brunner</name>
<name sortKey="Hua, Chenlei" sort="Hua, Chenlei" uniqKey="Hua C" first="Chenlei" last="Hua">Chenlei Hua</name>
<name sortKey="Wagener, Nadine" sort="Wagener, Nadine" uniqKey="Wagener N" first="Nadine" last="Wagener">Nadine Wagener</name>
</country>
<country name="République populaire de Chine"><noRegion><name sortKey="Zheng, Xiangzi" sort="Zheng, Xiangzi" uniqKey="Zheng X" first="Xiangzi" last="Zheng">Xiangzi Zheng</name>
</noRegion>
</country>
<country name="Royaume-Uni"><noRegion><name sortKey="Mclellan, Hazel" sort="Mclellan, Hazel" uniqKey="Mclellan H" first="Hazel" last="Mclellan">Hazel Mclellan</name>
</noRegion>
<name sortKey="Birch, Paul R J" sort="Birch, Paul R J" uniqKey="Birch P" first="Paul R J" last="Birch">Paul R J. Birch</name>
<name sortKey="Birch, Paul R J" sort="Birch, Paul R J" uniqKey="Birch P" first="Paul R J" last="Birch">Paul R J. Birch</name>
<name sortKey="Boevink, Petra C" sort="Boevink, Petra C" uniqKey="Boevink P" first="Petra C" last="Boevink">Petra C. Boevink</name>
</country>
<country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Brunner, Frederic" sort="Brunner, Frederic" uniqKey="Brunner F" first="Frédéric" last="Brunner">Frédéric Brunner</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000701 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000701 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PhytophthoraV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:29932222
   |texte=   Phytophthora infestans RXLR effector SFI5 requires association with calmodulin for PTI/MTI suppressing activity.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:29932222" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PhytophthoraV1

This area was generated with Dilib version V0.6.38.
Data generation: Fri Nov 20 11:20:57 2020. Site generation: Wed Mar 6 16:48:20 2024

	Serveur d'exploration Phytophthora
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration Phytophthora

Phytophthora infestans RXLR effector SFI5 requires association with calmodulin for PTI/MTI suppressing activity.

Phytophthora infestans RXLR effector SFI5 requires association with calmodulin for PTI/MTI suppressing activity.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki