Resistance Genes in Piper colubrinum: In Silico Survey From Leaf Transcriptome and Expression Studies Upon Challenge Inoculation with Phytophthora capsici.
Identifieur interne : 000676 ( Main/Exploration ); précédent : 000675; suivant : 000677Resistance Genes in Piper colubrinum: In Silico Survey From Leaf Transcriptome and Expression Studies Upon Challenge Inoculation with Phytophthora capsici.
Auteurs : Neema Malik [Inde] ; Johnson K. George [Inde]Source :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2018.
Descripteurs français
- KwdFr :
- Feuilles de plante (génétique), Feuilles de plante (microbiologie), Feuilles de plante (métabolisme), Maladies des plantes (microbiologie), Phytophthora (MeSH), Piper (génétique), Piper (microbiologie), Piper (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Résistance à la maladie (physiologie), Transcriptome (MeSH).
- MESH :
- génétique : Feuilles de plante, Piper.
- microbiologie : Feuilles de plante, Maladies des plantes, Piper.
- métabolisme : Feuilles de plante, Piper.
- physiologie : Résistance à la maladie.
- Phytophthora, Régulation de l'expression des gènes végétaux, Transcriptome.
English descriptors
- KwdEn :
- MESH :
- genetics : Piper, Plant Leaves.
- metabolism : Piper, Plant Leaves.
- microbiology : Piper, Plant Diseases, Plant Leaves.
- physiology : Disease Resistance.
- Gene Expression Regulation, Plant, Phytophthora, Transcriptome.
Abstract
The oomycetes, Phytophthora capsici, cause foot rot disease in black pepper. Piper colubrinum Link, a distant relative of cultivated black pepper, is highly resistant to this destructive pathogen. Identification of resistance (R) genes in P. colubrinum and the study of its expression profile during interaction with the pathogen can help in understanding the resistance mechanism involved. In the present study, 1289 R gene-related transcripts were mined from P. colubrinum transcriptome, clustered, and classified according to the conserved motifs and domains. Transcripts belonging to four major R gene classes were identified in P. colubrinum, but TIR-NBS-LRR-type R genes were absent. The relative expression of 12 selected R genes was studied using two virulent isolates of P. capsici, and these were found to be upregulated in the initial hours of plant pathogen interaction. The R genes studied were expressed even in aseptically maintained tissue-cultured plants and uninoculated greenhouse-grown plants at basal level suggesting that the plants are geared up with the R gene all the time and are under continuous surveillance for the pathogen and basal level of R gene expression do not require a pathogen trigger. ACT, ATUB, and EIF3E were identified as the most stable reference genes that can be used for real-time PCR study. The present study identified promising R genes in P. colubrinum which can be used in developing Phytophthora-resistant black pepper.
DOI: 10.1007/s12010-017-2600-7
PubMed: 28933036
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Resistance Genes in Piper colubrinum: In Silico Survey From Leaf Transcriptome and Expression Studies Upon Challenge Inoculation with Phytophthora capsici.</title><author><name sortKey="Malik, Neema" sort="Malik, Neema" uniqKey="Malik N" first="Neema" last="Malik">Neema Malik</name><affiliation wicri:level="1"><nlm:affiliation>ICAR-Indian Institute of Spices Research, Marikunnu P O, Kozhikode, 673012, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>ICAR-Indian Institute of Spices Research, Marikunnu P O, Kozhikode, 673012</wicri:regionArea><wicri:noRegion>673012</wicri:noRegion></affiliation></author><author><name sortKey="George, Johnson K" sort="George, Johnson K" uniqKey="George J" first="Johnson K" last="George">Johnson K. George</name><affiliation wicri:level="1"><nlm:affiliation>ICAR-Indian Institute of Spices Research, Marikunnu P O, Kozhikode, 673012, India. kokkatjohn@spices.res.in.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>ICAR-Indian Institute of Spices Research, Marikunnu P O, Kozhikode, 673012</wicri:regionArea><wicri:noRegion>673012</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:28933036</idno><idno type="pmid">28933036</idno><idno type="doi">10.1007/s12010-017-2600-7</idno><idno type="wicri:Area/Main/Corpus">000911</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000911</idno><idno type="wicri:Area/Main/Curation">000911</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000911</idno><idno type="wicri:Area/Main/Exploration">000911</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Resistance Genes in Piper colubrinum: In Silico Survey From Leaf Transcriptome and Expression Studies Upon Challenge Inoculation with Phytophthora capsici.</title><author><name sortKey="Malik, Neema" sort="Malik, Neema" uniqKey="Malik N" first="Neema" last="Malik">Neema Malik</name><affiliation wicri:level="1"><nlm:affiliation>ICAR-Indian Institute of Spices Research, Marikunnu P O, Kozhikode, 673012, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>ICAR-Indian Institute of Spices Research, Marikunnu P O, Kozhikode, 673012</wicri:regionArea><wicri:noRegion>673012</wicri:noRegion></affiliation></author><author><name sortKey="George, Johnson K" sort="George, Johnson K" uniqKey="George J" first="Johnson K" last="George">Johnson K. George</name><affiliation wicri:level="1"><nlm:affiliation>ICAR-Indian Institute of Spices Research, Marikunnu P O, Kozhikode, 673012, India. kokkatjohn@spices.res.in.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>ICAR-Indian Institute of Spices Research, Marikunnu P O, Kozhikode, 673012</wicri:regionArea><wicri:noRegion>673012</wicri:noRegion></affiliation></author></analytic><series><title level="j">Applied biochemistry and biotechnology</title><idno type="eISSN">1559-0291</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Disease Resistance (physiology)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Phytophthora (MeSH)</term><term>Piper (genetics)</term><term>Piper (metabolism)</term><term>Piper (microbiology)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (metabolism)</term><term>Plant Leaves (microbiology)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (microbiologie)</term><term>Feuilles de plante (métabolisme)</term><term>Maladies des plantes (microbiologie)</term><term>Phytophthora (MeSH)</term><term>Piper (génétique)</term><term>Piper (microbiologie)</term><term>Piper (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Résistance à la maladie (physiologie)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Piper</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Feuilles de plante</term><term>Piper</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Piper</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term><term>Piper</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Piper</term><term>Plant Diseases</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term><term>Piper</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Résistance à la maladie</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Disease Resistance</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Phytophthora</term><term>Transcriptome</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Phytophthora</term><term>Régulation de l'expression des gènes végétaux</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The oomycetes, Phytophthora capsici, cause foot rot disease in black pepper. Piper colubrinum Link, a distant relative of cultivated black pepper, is highly resistant to this destructive pathogen. Identification of resistance (R) genes in P. colubrinum and the study of its expression profile during interaction with the pathogen can help in understanding the resistance mechanism involved. In the present study, 1289 R gene-related transcripts were mined from P. colubrinum transcriptome, clustered, and classified according to the conserved motifs and domains. Transcripts belonging to four major R gene classes were identified in P. colubrinum, but TIR-NBS-LRR-type R genes were absent. The relative expression of 12 selected R genes was studied using two virulent isolates of P. capsici, and these were found to be upregulated in the initial hours of plant pathogen interaction. The R genes studied were expressed even in aseptically maintained tissue-cultured plants and uninoculated greenhouse-grown plants at basal level suggesting that the plants are geared up with the R gene all the time and are under continuous surveillance for the pathogen and basal level of R gene expression do not require a pathogen trigger. ACT, ATUB, and EIF3E were identified as the most stable reference genes that can be used for real-time PCR study. The present study identified promising R genes in P. colubrinum which can be used in developing Phytophthora-resistant black pepper.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28933036</PMID><DateCompleted><Year>2018</Year><Month>07</Month><Day>09</Day></DateCompleted><DateRevised><Year>2018</Year><Month>07</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1559-0291</ISSN><JournalIssue CitedMedium="Internet"><Volume>184</Volume><Issue>3</Issue><PubDate><Year>2018</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Applied biochemistry and biotechnology</Title><ISOAbbreviation>Appl Biochem Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Resistance Genes in Piper colubrinum: In Silico Survey From Leaf Transcriptome and Expression Studies Upon Challenge Inoculation with Phytophthora capsici.</ArticleTitle><Pagination><MedlinePgn>987-1008</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12010-017-2600-7</ELocationID><Abstract><AbstractText>The oomycetes, Phytophthora capsici, cause foot rot disease in black pepper. Piper colubrinum Link, a distant relative of cultivated black pepper, is highly resistant to this destructive pathogen. Identification of resistance (R) genes in P. colubrinum and the study of its expression profile during interaction with the pathogen can help in understanding the resistance mechanism involved. In the present study, 1289 R gene-related transcripts were mined from P. colubrinum transcriptome, clustered, and classified according to the conserved motifs and domains. Transcripts belonging to four major R gene classes were identified in P. colubrinum, but TIR-NBS-LRR-type R genes were absent. The relative expression of 12 selected R genes was studied using two virulent isolates of P. capsici, and these were found to be upregulated in the initial hours of plant pathogen interaction. The R genes studied were expressed even in aseptically maintained tissue-cultured plants and uninoculated greenhouse-grown plants at basal level suggesting that the plants are geared up with the R gene all the time and are under continuous surveillance for the pathogen and basal level of R gene expression do not require a pathogen trigger. ACT, ATUB, and EIF3E were identified as the most stable reference genes that can be used for real-time PCR study. The present study identified promising R genes in P. colubrinum which can be used in developing Phytophthora-resistant black pepper.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Malik</LastName><ForeName>Neema</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>ICAR-Indian Institute of Spices Research, Marikunnu P O, Kozhikode, 673012, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>George</LastName><ForeName>Johnson K</ForeName><Initials>JK</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-6289-073X</Identifier><AffiliationInfo><Affiliation>ICAR-Indian Institute of Spices Research, Marikunnu P O, Kozhikode, 673012, India. kokkatjohn@spices.res.in.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>09/757(0008)/2012-EMR-I</GrantID><Agency>Council of Scientific and Industrial Research</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>09</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Biochem Biotechnol</MedlineTA><NlmUniqueID>8208561</NlmUniqueID><ISSNLinking>0273-2289</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="Y">Phytophthora</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D031701" MajorTopicYN="Y">Piper</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="Y">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="Y">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Gene expression</Keyword><Keyword MajorTopicYN="N">Phytophthora capsici</Keyword><Keyword MajorTopicYN="N">Piper colubrinum</Keyword><Keyword MajorTopicYN="N">R gene</Keyword><Keyword MajorTopicYN="N">Real-time PCR</Keyword><Keyword MajorTopicYN="N">Reference genes</Keyword><Keyword MajorTopicYN="N">Resistance</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>11</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>09</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>9</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>9</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28933036</ArticleId><ArticleId IdType="doi">10.1007/s12010-017-2600-7</ArticleId><ArticleId IdType="pii">10.1007/s12010-017-2600-7</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Malik, Neema" sort="Malik, Neema" uniqKey="Malik N" first="Neema" last="Malik">Neema Malik</name></noRegion><name sortKey="George, Johnson K" sort="George, Johnson K" uniqKey="George J" first="Johnson K" last="George">Johnson K. George</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000676 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000676 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:28933036
|texte= Resistance Genes in Piper colubrinum: In Silico Survey From Leaf Transcriptome and Expression Studies Upon Challenge Inoculation with Phytophthora capsici.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:28933036" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |