Genetic and physical mapping of Avr1a in Phytophthora sojae.
Identifieur interne : 002697 ( Main/Corpus ); précédent : 002696; suivant : 002698Genetic and physical mapping of Avr1a in Phytophthora sojae.
Auteurs : Terry Macgregor ; Madan Bhattacharyya ; Brett Tyler ; Ravindra Bhat ; August F. Schmitthenner ; Mark GijzenSource :
- Genetics [ 0016-6731 ] ; 2002.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Algal Proteins, Fungal Proteins.
- genetics : Phytophthora.
- pathogenicity : Phytophthora.
- Chromosome Mapping, Chromosomes, Artificial, Bacterial, Crosses, Genetic, Genes, Dominant, Genes, Fungal, Genetic Markers, Virulence.
Abstract
The interaction between soybean and the phytopathogenic oomycete Phytophthora sojae is controlled by host resistance (Rps) genes and pathogen avirulence (Avr) genes. We have mapped the Avr1a locus in F(2) populations derived from four different P. sojae races. Four RAPD and nine AFLP markers linked to Avr1a were initially identified. Nine markers were used to compare genetic linkage maps of the Avr1a locus in two distinct F(2) populations. Distorted segregation ratios favoring homozygous genotypes were noted in both crosses. Segregation analysis of all the markers in one F(2) population of 90 progeny generated a map of 113.2 cM encompassing Avr1a, with one marker cosegregating with the gene. The cosegregating DNA marker was used to isolate P. sojae BAC clones and construct a physical map covering 170 kb, from which additional DNA markers were developed. Three markers occurring within the BAC contig were mapped in an enlarged population of 486 F(2) progeny. Avr1a was localized to a 114-kb interval, and an average physical to genetic distance ratio of 391 kb/cM was calculated for this region. This work provides a basis for the positional cloning of Avr1a.
PubMed: 11901113
PubMed Central: PMC1462023
Links to Exploration step
pubmed:11901113Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genetic and physical mapping of Avr1a in Phytophthora sojae.</title><author><name sortKey="Macgregor, Terry" sort="Macgregor, Terry" uniqKey="Macgregor T" first="Terry" last="Macgregor">Terry Macgregor</name><affiliation><nlm:affiliation>Agriculture and Agri-Food Canada, London, Ontario N5V 4T3, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Bhattacharyya, Madan" sort="Bhattacharyya, Madan" uniqKey="Bhattacharyya M" first="Madan" last="Bhattacharyya">Madan Bhattacharyya</name></author><author><name sortKey="Tyler, Brett" sort="Tyler, Brett" uniqKey="Tyler B" first="Brett" last="Tyler">Brett Tyler</name></author><author><name sortKey="Bhat, Ravindra" sort="Bhat, Ravindra" uniqKey="Bhat R" first="Ravindra" last="Bhat">Ravindra Bhat</name></author><author><name sortKey="Schmitthenner, August F" sort="Schmitthenner, August F" uniqKey="Schmitthenner A" first="August F" last="Schmitthenner">August F. Schmitthenner</name></author><author><name sortKey="Gijzen, Mark" sort="Gijzen, Mark" uniqKey="Gijzen M" first="Mark" last="Gijzen">Mark Gijzen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:11901113</idno><idno type="pmid">11901113</idno><idno type="pmc">PMC1462023</idno><idno type="wicri:Area/Main/Corpus">002697</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002697</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genetic and physical mapping of Avr1a in Phytophthora sojae.</title><author><name sortKey="Macgregor, Terry" sort="Macgregor, Terry" uniqKey="Macgregor T" first="Terry" last="Macgregor">Terry Macgregor</name><affiliation><nlm:affiliation>Agriculture and Agri-Food Canada, London, Ontario N5V 4T3, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Bhattacharyya, Madan" sort="Bhattacharyya, Madan" uniqKey="Bhattacharyya M" first="Madan" last="Bhattacharyya">Madan Bhattacharyya</name></author><author><name sortKey="Tyler, Brett" sort="Tyler, Brett" uniqKey="Tyler B" first="Brett" last="Tyler">Brett Tyler</name></author><author><name sortKey="Bhat, Ravindra" sort="Bhat, Ravindra" uniqKey="Bhat R" first="Ravindra" last="Bhat">Ravindra Bhat</name></author><author><name sortKey="Schmitthenner, August F" sort="Schmitthenner, August F" uniqKey="Schmitthenner A" first="August F" last="Schmitthenner">August F. Schmitthenner</name></author><author><name sortKey="Gijzen, Mark" sort="Gijzen, Mark" uniqKey="Gijzen M" first="Mark" last="Gijzen">Mark Gijzen</name></author></analytic><series><title level="j">Genetics</title><idno type="ISSN">0016-6731</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algal Proteins (genetics)</term><term>Chromosome Mapping (MeSH)</term><term>Chromosomes, Artificial, Bacterial (MeSH)</term><term>Crosses, Genetic (MeSH)</term><term>Fungal Proteins (genetics)</term><term>Genes, Dominant (MeSH)</term><term>Genes, Fungal (MeSH)</term><term>Genetic Markers (MeSH)</term><term>Phytophthora (genetics)</term><term>Phytophthora (pathogenicity)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Algal Proteins</term><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromosome Mapping</term><term>Chromosomes, Artificial, Bacterial</term><term>Crosses, Genetic</term><term>Genes, Dominant</term><term>Genes, Fungal</term><term>Genetic Markers</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The interaction between soybean and the phytopathogenic oomycete Phytophthora sojae is controlled by host resistance (Rps) genes and pathogen avirulence (Avr) genes. We have mapped the Avr1a locus in F(2) populations derived from four different P. sojae races. Four RAPD and nine AFLP markers linked to Avr1a were initially identified. Nine markers were used to compare genetic linkage maps of the Avr1a locus in two distinct F(2) populations. Distorted segregation ratios favoring homozygous genotypes were noted in both crosses. Segregation analysis of all the markers in one F(2) population of 90 progeny generated a map of 113.2 cM encompassing Avr1a, with one marker cosegregating with the gene. The cosegregating DNA marker was used to isolate P. sojae BAC clones and construct a physical map covering 170 kb, from which additional DNA markers were developed. Three markers occurring within the BAC contig were mapped in an enlarged population of 486 F(2) progeny. Avr1a was localized to a 114-kb interval, and an average physical to genetic distance ratio of 391 kb/cM was calculated for this region. This work provides a basis for the positional cloning of Avr1a.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11901113</PMID><DateCompleted><Year>2002</Year><Month>09</Month><Day>06</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0016-6731</ISSN><JournalIssue CitedMedium="Print"><Volume>160</Volume><Issue>3</Issue><PubDate><Year>2002</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Genetics</Title><ISOAbbreviation>Genetics</ISOAbbreviation></Journal><ArticleTitle>Genetic and physical mapping of Avr1a in Phytophthora sojae.</ArticleTitle><Pagination><MedlinePgn>949-59</MedlinePgn></Pagination><Abstract><AbstractText>The interaction between soybean and the phytopathogenic oomycete Phytophthora sojae is controlled by host resistance (Rps) genes and pathogen avirulence (Avr) genes. We have mapped the Avr1a locus in F(2) populations derived from four different P. sojae races. Four RAPD and nine AFLP markers linked to Avr1a were initially identified. Nine markers were used to compare genetic linkage maps of the Avr1a locus in two distinct F(2) populations. Distorted segregation ratios favoring homozygous genotypes were noted in both crosses. Segregation analysis of all the markers in one F(2) population of 90 progeny generated a map of 113.2 cM encompassing Avr1a, with one marker cosegregating with the gene. The cosegregating DNA marker was used to isolate P. sojae BAC clones and construct a physical map covering 170 kb, from which additional DNA markers were developed. Three markers occurring within the BAC contig were mapped in an enlarged population of 486 F(2) progeny. Avr1a was localized to a 114-kb interval, and an average physical to genetic distance ratio of 391 kb/cM was calculated for this region. This work provides a basis for the positional cloning of Avr1a.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>MacGregor</LastName><ForeName>Terry</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Agriculture and Agri-Food Canada, London, Ontario N5V 4T3, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bhattacharyya</LastName><ForeName>Madan</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Tyler</LastName><ForeName>Brett</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Bhat</LastName><ForeName>Ravindra</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Schmitthenner</LastName><ForeName>August F</ForeName><Initials>AF</Initials></Author><Author ValidYN="Y"><LastName>Gijzen</LastName><ForeName>Mark</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Genetics</MedlineTA><NlmUniqueID>0374636</NlmUniqueID><ISSNLinking>0016-6731</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020418">Algal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005819">Genetic Markers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020418" MajorTopicYN="N">Algal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002874" MajorTopicYN="Y">Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D022202" MajorTopicYN="N">Chromosomes, Artificial, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003433" MajorTopicYN="N">Crosses, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005799" MajorTopicYN="N">Genes, Dominant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="Y">Genes, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005819" MajorTopicYN="N">Genetic Markers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000472" 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Jan 1;28(1):87-90</Citation><ArticleIdList><ArticleId IdType="pubmed">10592189</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 1996;34:153-79</Citation><ArticleIdList><ArticleId IdType="pubmed">15012539</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9828-32</Citation><ArticleIdList><ArticleId IdType="pubmed">1682921</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1995 Nov 11;23(21):4407-14</Citation><ArticleIdList><ArticleId IdType="pubmed">7501463</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 1999 Nov;28(2):94-106</Citation><ArticleIdList><ArticleId IdType="pubmed">10587472</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1990 Nov 25;18(22):6531-5</Citation><ArticleIdList><ArticleId IdType="pubmed">1979162</ArticleId></ArticleIdList></Reference><Reference><Citation>Genomics. 1987 Oct;1(2):174-81</Citation><ArticleIdList><ArticleId IdType="pubmed">3692487</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Genet. 1994 Dec;27(1):77-82</Citation><ArticleIdList><ArticleId IdType="pubmed">7750150</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Microbiol. 1999 Aug;2(4):368-73</Citation><ArticleIdList><ArticleId IdType="pubmed">10458978</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1990 Dec 25;18(24):7213-8</Citation><ArticleIdList><ArticleId IdType="pubmed">2259619</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 1997 Jun;21(3):278-91</Citation><ArticleIdList><ArticleId IdType="pubmed">9299198</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1998 Sep;10(9):1413-26</Citation><ArticleIdList><ArticleId IdType="pubmed">9724689</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1993 Aug;4(2):403-10</Citation><ArticleIdList><ArticleId IdType="pubmed">8106085</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 1995 Nov-Dec;8(6):988-95</Citation><ArticleIdList><ArticleId IdType="pubmed">8664507</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):16-20</Citation><ArticleIdList><ArticleId IdType="pubmed">8419918</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1994 Feb;24(4):685-7</Citation><ArticleIdList><ArticleId IdType="pubmed">7908836</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2000 May;123(1):243-54</Citation><ArticleIdList><ArticleId IdType="pubmed">10806241</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Genet Biol. 1998 Dec;25(3):181-95</Citation><ArticleIdList><ArticleId IdType="pubmed">9917372</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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