The chloroplast protein RPH1 plays a role in the immune response of Arabidopsis to Phytophthora brassicae.
Identifieur interne : 001B65 ( Main/Corpus ); précédent : 001B64; suivant : 001B66The chloroplast protein RPH1 plays a role in the immune response of Arabidopsis to Phytophthora brassicae.
Auteurs : Khaoula Belhaj ; Baiqing Lin ; Felix MauchSource :
- The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2009.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Arabidopsis (genetics), Arabidopsis (immunology), Arabidopsis (metabolism), Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Cell Death (MeSH), Chloroplasts (genetics), Chloroplasts (metabolism), DNA, Plant (genetics), Gene Expression Regulation, Plant (MeSH), Gene Silencing (MeSH), Genetic Complementation Test (MeSH), Hydrogen Peroxide (MeSH), Molecular Sequence Data (MeSH), Mutagenesis, Insertional (MeSH), Oxidative Stress (MeSH), Phytophthora (growth & development), Plant Diseases (genetics), Plant Diseases (immunology), Sequence Alignment (MeSH), Sequence Analysis, DNA (MeSH), Solanum tuberosum (genetics), Solanum tuberosum (metabolism).
- MESH :
- chemical , genetics : Arabidopsis Proteins, DNA, Plant.
- genetics : Arabidopsis, Chloroplasts, Plant Diseases, Solanum tuberosum.
- growth & development : Phytophthora.
- immunology : Arabidopsis, Plant Diseases.
- metabolism : Arabidopsis, Arabidopsis Proteins, Chloroplasts, Solanum tuberosum.
- Amino Acid Sequence, Cell Death, Gene Expression Regulation, Plant, Gene Silencing, Genetic Complementation Test, Hydrogen Peroxide, Molecular Sequence Data, Mutagenesis, Insertional, Oxidative Stress, Sequence Alignment, Sequence Analysis, DNA.
Abstract
Plant immune responses to pathogens are often associated with enhanced production of reactive oxygen species (ROS), known as the oxidative burst, and with rapid hypersensitive host cell death (the hypersensitive response, HR) at sites of attempted infection. It is generally accepted that the oxidative burst acts as a promotive signal for HR, and that HR is highly correlated with efficient disease resistance. We have identified the Arabidopsis mutant rph1 (resistance to Phytophthora 1), which is susceptible to the oomycete pathogen Phytophthora brassicae despite rapid induction of HR. The susceptibility of rph1 was specific for P. brassicae and coincided with a reduced oxidative burst, a runaway cell-death response, and failure to properly activate the expression of defence-related genes. From these results, we conclude that, in the immune response to P. brassicae, (i) HR is not sufficient to stop the pathogen, (ii) HR initiation can occur in the absence of a major oxidative burst, (iii) the oxidative burst plays a role in limiting the spread of cell death, and (iv) RPH1 is a positive regulator of the P. brassicae-induced oxidative burst and enhanced expression of defence-related genes. Surprisingly, RPH1 encodes an evolutionary highly conserved chloroplast protein, indicating a function of this organelle in activation of a subset of immune reactions in response to P. brassicae. The disease resistance-related role of RPH1 was not limited to the Arabidopsis model system. Silencing of the potato homolog StRPH1 in a resistant potato cultivar caused susceptibility to the late blight pathogen Phytophthora infestans.
DOI: 10.1111/j.1365-313X.2008.03779.x
PubMed: 19170932
Links to Exploration step
pubmed:19170932Le document en format XML
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<author><name sortKey="Belhaj, Khaoula" sort="Belhaj, Khaoula" uniqKey="Belhaj K" first="Khaoula" last="Belhaj">Khaoula Belhaj</name>
<affiliation><nlm:affiliation>Department of Biology, University of Fribourg, Fribourg, Switzerland.</nlm:affiliation>
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<author><name sortKey="Lin, Baiqing" sort="Lin, Baiqing" uniqKey="Lin B" first="Baiqing" last="Lin">Baiqing Lin</name>
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<author><name sortKey="Mauch, Felix" sort="Mauch, Felix" uniqKey="Mauch F" first="Felix" last="Mauch">Felix Mauch</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Arabidopsis (genetics)</term>
<term>Arabidopsis (immunology)</term>
<term>Arabidopsis (metabolism)</term>
<term>Arabidopsis Proteins (genetics)</term>
<term>Arabidopsis Proteins (metabolism)</term>
<term>Cell Death (MeSH)</term>
<term>Chloroplasts (genetics)</term>
<term>Chloroplasts (metabolism)</term>
<term>DNA, Plant (genetics)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Gene Silencing (MeSH)</term>
<term>Genetic Complementation Test (MeSH)</term>
<term>Hydrogen Peroxide (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Mutagenesis, Insertional (MeSH)</term>
<term>Oxidative Stress (MeSH)</term>
<term>Phytophthora (growth & development)</term>
<term>Plant Diseases (genetics)</term>
<term>Plant Diseases (immunology)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Solanum tuberosum (genetics)</term>
<term>Solanum tuberosum (metabolism)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Arabidopsis Proteins</term>
<term>DNA, Plant</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term>
<term>Chloroplasts</term>
<term>Plant Diseases</term>
<term>Solanum tuberosum</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Arabidopsis</term>
<term>Plant Diseases</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term>
<term>Arabidopsis Proteins</term>
<term>Chloroplasts</term>
<term>Solanum tuberosum</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Cell Death</term>
<term>Gene Expression Regulation, Plant</term>
<term>Gene Silencing</term>
<term>Genetic Complementation Test</term>
<term>Hydrogen Peroxide</term>
<term>Molecular Sequence Data</term>
<term>Mutagenesis, Insertional</term>
<term>Oxidative Stress</term>
<term>Sequence Alignment</term>
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<front><div type="abstract" xml:lang="en">Plant immune responses to pathogens are often associated with enhanced production of reactive oxygen species (ROS), known as the oxidative burst, and with rapid hypersensitive host cell death (the hypersensitive response, HR) at sites of attempted infection. It is generally accepted that the oxidative burst acts as a promotive signal for HR, and that HR is highly correlated with efficient disease resistance. We have identified the Arabidopsis mutant rph1 (resistance to Phytophthora 1), which is susceptible to the oomycete pathogen Phytophthora brassicae despite rapid induction of HR. The susceptibility of rph1 was specific for P. brassicae and coincided with a reduced oxidative burst, a runaway cell-death response, and failure to properly activate the expression of defence-related genes. From these results, we conclude that, in the immune response to P. brassicae, (i) HR is not sufficient to stop the pathogen, (ii) HR initiation can occur in the absence of a major oxidative burst, (iii) the oxidative burst plays a role in limiting the spread of cell death, and (iv) RPH1 is a positive regulator of the P. brassicae-induced oxidative burst and enhanced expression of defence-related genes. Surprisingly, RPH1 encodes an evolutionary highly conserved chloroplast protein, indicating a function of this organelle in activation of a subset of immune reactions in response to P. brassicae. The disease resistance-related role of RPH1 was not limited to the Arabidopsis model system. Silencing of the potato homolog StRPH1 in a resistant potato cultivar caused susceptibility to the late blight pathogen Phytophthora infestans.</div>
</front>
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<Title>The Plant journal : for cell and molecular biology</Title>
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<ArticleTitle>The chloroplast protein RPH1 plays a role in the immune response of Arabidopsis to Phytophthora brassicae.</ArticleTitle>
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<Abstract><AbstractText>Plant immune responses to pathogens are often associated with enhanced production of reactive oxygen species (ROS), known as the oxidative burst, and with rapid hypersensitive host cell death (the hypersensitive response, HR) at sites of attempted infection. It is generally accepted that the oxidative burst acts as a promotive signal for HR, and that HR is highly correlated with efficient disease resistance. We have identified the Arabidopsis mutant rph1 (resistance to Phytophthora 1), which is susceptible to the oomycete pathogen Phytophthora brassicae despite rapid induction of HR. The susceptibility of rph1 was specific for P. brassicae and coincided with a reduced oxidative burst, a runaway cell-death response, and failure to properly activate the expression of defence-related genes. From these results, we conclude that, in the immune response to P. brassicae, (i) HR is not sufficient to stop the pathogen, (ii) HR initiation can occur in the absence of a major oxidative burst, (iii) the oxidative burst plays a role in limiting the spread of cell death, and (iv) RPH1 is a positive regulator of the P. brassicae-induced oxidative burst and enhanced expression of defence-related genes. Surprisingly, RPH1 encodes an evolutionary highly conserved chloroplast protein, indicating a function of this organelle in activation of a subset of immune reactions in response to P. brassicae. The disease resistance-related role of RPH1 was not limited to the Arabidopsis model system. Silencing of the potato homolog StRPH1 in a resistant potato cultivar caused susceptibility to the late blight pathogen Phytophthora infestans.</AbstractText>
</Abstract>
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<ForeName>Khaoula</ForeName>
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<AffiliationInfo><Affiliation>Department of Biology, University of Fribourg, Fribourg, Switzerland.</Affiliation>
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