Cloning and characterization of r3b; members of the r3 superfamily of late blight resistance genes show sequence and functional divergence.
Identifieur interne : 001692 ( Main/Corpus ); précédent : 001691; suivant : 001693Cloning and characterization of r3b; members of the r3 superfamily of late blight resistance genes show sequence and functional divergence.
Auteurs : Guangcun Li ; Sanwen Huang ; Xiao Guo ; Ying Li ; Yu Yang ; Zhen Guo ; Hanhui Kuang ; Hendrik Rietman ; Marjan Bergervoet ; Vivianne G G A. Vleeshouwers ; Edwin A G. Van Der Vossen ; Dongyu Qu ; Richard G F. Visser ; Evert Jacobsen ; Jack H. VossenSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2011.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Base Sequence (MeSH), Chromosome Mapping (MeSH), Chromosomes, Plant (genetics), Cloning, Molecular (MeSH), DNA, Plant (genetics), Genes, Plant (MeSH), Genetic Complementation Test (MeSH), Host-Pathogen Interactions (genetics), Multigene Family (MeSH), Phylogeny (MeSH), Phytophthora infestans (pathogenicity), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Diseases (prevention & control), Plant Leaves (microbiology), Plants, Genetically Modified (MeSH), Solanum tuberosum (genetics), Solanum tuberosum (microbiology), Tobacco (genetics), Tobacco (microbiology), Transformation, Genetic (MeSH), Virulence (MeSH).
- MESH :
- chemical , genetics : DNA, Plant.
- genetics : Agrobacterium tumefaciens, Chromosomes, Plant, Host-Pathogen Interactions, Plant Diseases, Solanum tuberosum, Tobacco.
- microbiology : Plant Diseases, Plant Leaves, Solanum tuberosum, Tobacco.
- pathogenicity : Phytophthora infestans.
- prevention & control : Plant Diseases.
- Base Sequence, Chromosome Mapping, Cloning, Molecular, Genes, Plant, Genetic Complementation Test, Multigene Family, Phylogeny, Plants, Genetically Modified, Transformation, Genetic, Virulence.
Abstract
Massive resistance (R) gene stacking is considered to be one of the most promising approaches to provide durable resistance to potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in potato is an example of natural R gene stacking, because it contains two closely linked R genes (R3a and R3b) with distinct resistance specificities to Phytophthora infestans. Here, we report about the positional cloning of R3b. Both transient and stable transformations of susceptible tobacco and potato plants showed that R3b conferred full resistance to incompatible P. infestans isolates. R3b encodes a coiled-coil nucleotide-binding site leucine-rich repeat protein and exhibits 82% nucleotide identity with R3a located in the same R3 cluster. The R3b gene specifically recognizes Avr3b, a newly identified avirulence factor from P. infestans. R3b does not recognize Avr3a, the corresponding avirulence gene for R3a, showing that, despite their high sequence similarity, R3b and R3a have clearly distinct recognition specificities. In addition to the Rpi-mcd1/Rpi-blb3 locus on chromosome IV, the R3 locus on chromosome XI is the second example of an R-gene cluster with multiple genes recognizing different races of P. infestans.
DOI: 10.1094/MPMI-11-10-0276
PubMed: 21649512
Links to Exploration step
pubmed:21649512Le document en format XML
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China.</nlm:affiliation></affiliation></author><author><name sortKey="Huang, Sanwen" sort="Huang, Sanwen" uniqKey="Huang S" first="Sanwen" last="Huang">Sanwen Huang</name></author><author><name sortKey="Guo, Xiao" sort="Guo, Xiao" uniqKey="Guo X" first="Xiao" last="Guo">Xiao Guo</name></author><author><name sortKey="Li, Ying" sort="Li, Ying" uniqKey="Li Y" first="Ying" last="Li">Ying Li</name></author><author><name sortKey="Yang, Yu" sort="Yang, Yu" uniqKey="Yang Y" first="Yu" last="Yang">Yu Yang</name></author><author><name sortKey="Guo, Zhen" sort="Guo, Zhen" uniqKey="Guo Z" first="Zhen" last="Guo">Zhen Guo</name></author><author><name sortKey="Kuang, Hanhui" sort="Kuang, Hanhui" uniqKey="Kuang H" first="Hanhui" last="Kuang">Hanhui Kuang</name></author><author><name sortKey="Rietman, Hendrik" sort="Rietman, Hendrik" uniqKey="Rietman H" first="Hendrik" last="Rietman">Hendrik Rietman</name></author><author><name sortKey="Bergervoet, Marjan" sort="Bergervoet, Marjan" uniqKey="Bergervoet M" first="Marjan" last="Bergervoet">Marjan Bergervoet</name></author><author><name sortKey="Vleeshouwers, Vivianne G G A" sort="Vleeshouwers, Vivianne G G A" uniqKey="Vleeshouwers V" first="Vivianne G G A" last="Vleeshouwers">Vivianne G G A. Vleeshouwers</name></author><author><name sortKey="Van Der Vossen, Edwin A G" sort="Van Der Vossen, Edwin A G" uniqKey="Van Der Vossen E" first="Edwin A G" last="Van Der Vossen">Edwin A G. Van Der Vossen</name></author><author><name sortKey="Qu, Dongyu" sort="Qu, Dongyu" uniqKey="Qu D" first="Dongyu" last="Qu">Dongyu Qu</name></author><author><name sortKey="Visser, Richard G F" sort="Visser, Richard G F" uniqKey="Visser R" first="Richard G F" last="Visser">Richard G F. Visser</name></author><author><name sortKey="Jacobsen, Evert" sort="Jacobsen, Evert" uniqKey="Jacobsen E" first="Evert" last="Jacobsen">Evert Jacobsen</name></author><author><name sortKey="Vossen, Jack H" sort="Vossen, Jack H" uniqKey="Vossen J" first="Jack H" last="Vossen">Jack H. Vossen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21649512</idno><idno type="pmid">21649512</idno><idno type="doi">10.1094/MPMI-11-10-0276</idno><idno type="wicri:Area/Main/Corpus">001692</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001692</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning and characterization of r3b; members of the r3 superfamily of late blight resistance genes show sequence and functional divergence.</title><author><name sortKey="Li, Guangcun" sort="Li, Guangcun" uniqKey="Li G" first="Guangcun" last="Li">Guangcun Li</name><affiliation><nlm:affiliation>key Laboratory of Corp Genetic Improvement and Biotechnology, Shandong Province, Shandong Academy of Agricultural Sciences, Jinan 250100, P.R. China.</nlm:affiliation></affiliation></author><author><name sortKey="Huang, Sanwen" sort="Huang, Sanwen" uniqKey="Huang S" first="Sanwen" last="Huang">Sanwen Huang</name></author><author><name sortKey="Guo, Xiao" sort="Guo, Xiao" uniqKey="Guo X" first="Xiao" last="Guo">Xiao Guo</name></author><author><name sortKey="Li, Ying" sort="Li, Ying" uniqKey="Li Y" first="Ying" last="Li">Ying Li</name></author><author><name sortKey="Yang, Yu" sort="Yang, Yu" uniqKey="Yang Y" first="Yu" last="Yang">Yu Yang</name></author><author><name sortKey="Guo, Zhen" sort="Guo, Zhen" uniqKey="Guo Z" first="Zhen" last="Guo">Zhen Guo</name></author><author><name sortKey="Kuang, Hanhui" sort="Kuang, Hanhui" uniqKey="Kuang H" first="Hanhui" last="Kuang">Hanhui Kuang</name></author><author><name sortKey="Rietman, Hendrik" sort="Rietman, Hendrik" uniqKey="Rietman H" first="Hendrik" last="Rietman">Hendrik Rietman</name></author><author><name sortKey="Bergervoet, Marjan" sort="Bergervoet, Marjan" uniqKey="Bergervoet M" first="Marjan" last="Bergervoet">Marjan Bergervoet</name></author><author><name sortKey="Vleeshouwers, Vivianne G G A" sort="Vleeshouwers, Vivianne G G A" uniqKey="Vleeshouwers V" first="Vivianne G G A" last="Vleeshouwers">Vivianne G G A. Vleeshouwers</name></author><author><name sortKey="Van Der Vossen, Edwin A G" sort="Van Der Vossen, Edwin A G" uniqKey="Van Der Vossen E" first="Edwin A G" last="Van Der Vossen">Edwin A G. Van Der Vossen</name></author><author><name sortKey="Qu, Dongyu" sort="Qu, Dongyu" uniqKey="Qu D" first="Dongyu" last="Qu">Dongyu Qu</name></author><author><name sortKey="Visser, Richard G F" sort="Visser, Richard G F" uniqKey="Visser R" first="Richard G F" last="Visser">Richard G F. Visser</name></author><author><name sortKey="Jacobsen, Evert" sort="Jacobsen, Evert" uniqKey="Jacobsen E" first="Evert" last="Jacobsen">Evert Jacobsen</name></author><author><name sortKey="Vossen, Jack H" sort="Vossen, Jack H" uniqKey="Vossen J" first="Jack H" last="Vossen">Jack H. Vossen</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Base Sequence (MeSH)</term><term>Chromosome Mapping (MeSH)</term><term>Chromosomes, Plant (genetics)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Plant (genetics)</term><term>Genes, Plant (MeSH)</term><term>Genetic Complementation Test (MeSH)</term><term>Host-Pathogen Interactions (genetics)</term><term>Multigene Family (MeSH)</term><term>Phylogeny (MeSH)</term><term>Phytophthora infestans (pathogenicity)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Diseases (prevention & control)</term><term>Plant Leaves (microbiology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Solanum tuberosum (genetics)</term><term>Solanum tuberosum (microbiology)</term><term>Tobacco (genetics)</term><term>Tobacco (microbiology)</term><term>Transformation, Genetic (MeSH)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Plant</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Chromosomes, Plant</term><term>Host-Pathogen Interactions</term><term>Plant Diseases</term><term>Solanum tuberosum</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Solanum tuberosum</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Chromosome Mapping</term><term>Cloning, Molecular</term><term>Genes, Plant</term><term>Genetic Complementation Test</term><term>Multigene Family</term><term>Phylogeny</term><term>Plants, Genetically Modified</term><term>Transformation, Genetic</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Massive resistance (R) gene stacking is considered to be one of the most promising approaches to provide durable resistance to potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in potato is an example of natural R gene stacking, because it contains two closely linked R genes (R3a and R3b) with distinct resistance specificities to Phytophthora infestans. Here, we report about the positional cloning of R3b. Both transient and stable transformations of susceptible tobacco and potato plants showed that R3b conferred full resistance to incompatible P. infestans isolates. R3b encodes a coiled-coil nucleotide-binding site leucine-rich repeat protein and exhibits 82% nucleotide identity with R3a located in the same R3 cluster. The R3b gene specifically recognizes Avr3b, a newly identified avirulence factor from P. infestans. R3b does not recognize Avr3a, the corresponding avirulence gene for R3a, showing that, despite their high sequence similarity, R3b and R3a have clearly distinct recognition specificities. In addition to the Rpi-mcd1/Rpi-blb3 locus on chromosome IV, the R3 locus on chromosome XI is the second example of an R-gene cluster with multiple genes recognizing different races of P. infestans.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21649512</PMID><DateCompleted><Year>2011</Year><Month>10</Month><Day>25</Day></DateCompleted><DateRevised><Year>2011</Year><Month>11</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>10</Issue><PubDate><Year>2011</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Cloning and characterization of r3b; members of the r3 superfamily of late blight resistance genes show sequence and functional divergence.</ArticleTitle><Pagination><MedlinePgn>1132-42</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-11-10-0276</ELocationID><Abstract><AbstractText>Massive resistance (R) gene stacking is considered to be one of the most promising approaches to provide durable resistance to potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in potato is an example of natural R gene stacking, because it contains two closely linked R genes (R3a and R3b) with distinct resistance specificities to Phytophthora infestans. Here, we report about the positional cloning of R3b. Both transient and stable transformations of susceptible tobacco and potato plants showed that R3b conferred full resistance to incompatible P. infestans isolates. R3b encodes a coiled-coil nucleotide-binding site leucine-rich repeat protein and exhibits 82% nucleotide identity with R3a located in the same R3 cluster. The R3b gene specifically recognizes Avr3b, a newly identified avirulence factor from P. infestans. R3b does not recognize Avr3a, the corresponding avirulence gene for R3a, showing that, despite their high sequence similarity, R3b and R3a have clearly distinct recognition specificities. In addition to the Rpi-mcd1/Rpi-blb3 locus on chromosome IV, the R3 locus on chromosome XI is the second example of an R-gene cluster with multiple genes recognizing different races of P. infestans.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Guangcun</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>key Laboratory of Corp Genetic Improvement and Biotechnology, Shandong Province, Shandong Academy of Agricultural Sciences, Jinan 250100, P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Sanwen</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Xiao</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Ying</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yu</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Zhen</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Kuang</LastName><ForeName>Hanhui</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Rietman</LastName><ForeName>Hendrik</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Bergervoet</LastName><ForeName>Marjan</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Vleeshouwers</LastName><ForeName>Vivianne G G A</ForeName><Initials>VG</Initials></Author><Author ValidYN="Y"><LastName>van der Vossen</LastName><ForeName>Edwin A G</ForeName><Initials>EA</Initials></Author><Author ValidYN="Y"><LastName>Qu</LastName><ForeName>Dongyu</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Visser</LastName><ForeName>Richard G F</ForeName><Initials>RG</Initials></Author><Author ValidYN="Y"><LastName>Jacobsen</LastName><ForeName>Evert</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Vossen</LastName><ForeName>Jack H</ForeName><Initials>JH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002874" MajorTopicYN="N">Chromosome Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032461" MajorTopicYN="N">Chromosomes, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005816" MajorTopicYN="N">Genetic Complementation Test</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005810" MajorTopicYN="N">Multigene Family</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName><QualifierName UI="Q000517" MajorTopicYN="N">prevention & control</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>6</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>6</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>10</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21649512</ArticleId><ArticleId 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