The RxLR effector Avh241 from Phytophthora sojae requires plasma membrane localization to induce plant cell death.
Identifieur interne : 001472 ( Main/Corpus ); précédent : 001471; suivant : 001473The RxLR effector Avh241 from Phytophthora sojae requires plasma membrane localization to induce plant cell death.
Auteurs : Xiaoli Yu ; Junli Tang ; Qunqing Wang ; Wenwu Ye ; Kai Tao ; Shuyi Duan ; Chenchen Lu ; Xinyu Yang ; Suomeng Dong ; Xiaobo Zheng ; Yuanchao WangSource :
- The New phytologist [ 1469-8137 ] ; 2012.
English descriptors
- KwdEn :
- Amino Acid Motifs (MeSH), Amino Acid Sequence (MeSH), Cell Death (MeSH), Cell Membrane (metabolism), Gene Deletion (MeSH), Gene Silencing (MeSH), Mitogen-Activated Protein Kinases (metabolism), Molecular Sequence Data (MeSH), Phytophthora (metabolism), Phytophthora (pathogenicity), Plant Cells (microbiology), Plant Diseases (microbiology), Plant Immunity (MeSH), Protein Structure, Tertiary (MeSH), Protein Transport (MeSH), Proteins (chemistry), Proteins (metabolism), Species Specificity (MeSH), Subcellular Fractions (metabolism), Tobacco (cytology), Tobacco (enzymology), Tobacco (microbiology), Virulence (MeSH).
- MESH :
- chemical , chemistry : Proteins.
- chemical , metabolism : Mitogen-Activated Protein Kinases, Proteins.
- cytology : Tobacco.
- enzymology : Tobacco.
- metabolism : Cell Membrane, Phytophthora, Subcellular Fractions.
- microbiology : Plant Cells, Plant Diseases, Tobacco.
- pathogenicity : Phytophthora.
- Amino Acid Motifs, Amino Acid Sequence, Cell Death, Gene Deletion, Gene Silencing, Molecular Sequence Data, Plant Immunity, Protein Structure, Tertiary, Protein Transport, Species Specificity, Virulence.
Abstract
• The Phytophthora sojae genome encodes hundreds of RxLR effectors predicted to manipulate various plant defense responses, but the molecular mechanisms involved are largely unknown. Here we have characterized in detail the P. sojae RxLR effector Avh241. • To determine the function and localization of Avh241, we transiently expressed it on different plants. Silencing of Avh241 in P. sojae, we determined its virulence during infection. Through the assay of promoting infection by Phytophthora capsici to Nicotiana benthamiana, we further confirmed this virulence role. • Avh241 induced cell death in several different plants and localized to the plant plasma membrane. An N-terminal motif within Avh241 was important for membrane localization and cell death-inducing activity. Two mitogen-activated protein kinases, NbMEK2 and NbWIPK, were required for the cell death triggered by Avh241 in N. benthamiana. Avh241 was important for the pathogen's full virulence on soybean. Avh241 could also promote infection by P. capsici and the membrane localization motif was not required to promote infection. • This work suggests that Avh241 interacts with the plant immune system via at least two different mechanisms, one recognized by plants dependent on subcellular localization and one promoting infection independent on membrane localization.
DOI: 10.1111/j.1469-8137.2012.04241.x
PubMed: 22816601
Links to Exploration step
pubmed:22816601Le document en format XML
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last="Tao">Kai Tao</name></author><author><name sortKey="Duan, Shuyi" sort="Duan, Shuyi" uniqKey="Duan S" first="Shuyi" last="Duan">Shuyi Duan</name></author><author><name sortKey="Lu, Chenchen" sort="Lu, Chenchen" uniqKey="Lu C" first="Chenchen" last="Lu">Chenchen Lu</name></author><author><name sortKey="Yang, Xinyu" sort="Yang, Xinyu" uniqKey="Yang X" first="Xinyu" last="Yang">Xinyu Yang</name></author><author><name sortKey="Dong, Suomeng" sort="Dong, Suomeng" uniqKey="Dong S" first="Suomeng" last="Dong">Suomeng Dong</name></author><author><name sortKey="Zheng, Xiaobo" sort="Zheng, Xiaobo" uniqKey="Zheng X" first="Xiaobo" last="Zheng">Xiaobo Zheng</name></author><author><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22816601</idno><idno type="pmid">22816601</idno><idno 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Wang</name></author><author><name sortKey="Ye, Wenwu" sort="Ye, Wenwu" uniqKey="Ye W" first="Wenwu" last="Ye">Wenwu Ye</name></author><author><name sortKey="Tao, Kai" sort="Tao, Kai" uniqKey="Tao K" first="Kai" last="Tao">Kai Tao</name></author><author><name sortKey="Duan, Shuyi" sort="Duan, Shuyi" uniqKey="Duan S" first="Shuyi" last="Duan">Shuyi Duan</name></author><author><name sortKey="Lu, Chenchen" sort="Lu, Chenchen" uniqKey="Lu C" first="Chenchen" last="Lu">Chenchen Lu</name></author><author><name sortKey="Yang, Xinyu" sort="Yang, Xinyu" uniqKey="Yang X" first="Xinyu" last="Yang">Xinyu Yang</name></author><author><name sortKey="Dong, Suomeng" sort="Dong, Suomeng" uniqKey="Dong S" first="Suomeng" last="Dong">Suomeng Dong</name></author><author><name sortKey="Zheng, Xiaobo" sort="Zheng, Xiaobo" uniqKey="Zheng X" first="Xiaobo" last="Zheng">Xiaobo Zheng</name></author><author><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs (MeSH)</term><term>Amino Acid Sequence (MeSH)</term><term>Cell Death (MeSH)</term><term>Cell Membrane (metabolism)</term><term>Gene Deletion (MeSH)</term><term>Gene Silencing (MeSH)</term><term>Mitogen-Activated Protein Kinases (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Phytophthora (metabolism)</term><term>Phytophthora (pathogenicity)</term><term>Plant Cells (microbiology)</term><term>Plant Diseases (microbiology)</term><term>Plant Immunity (MeSH)</term><term>Protein Structure, Tertiary (MeSH)</term><term>Protein Transport (MeSH)</term><term>Proteins (chemistry)</term><term>Proteins (metabolism)</term><term>Species Specificity (MeSH)</term><term>Subcellular Fractions (metabolism)</term><term>Tobacco (cytology)</term><term>Tobacco (enzymology)</term><term>Tobacco (microbiology)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mitogen-Activated Protein Kinases</term><term>Proteins</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Membrane</term><term>Phytophthora</term><term>Subcellular Fractions</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Cells</term><term>Plant Diseases</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Motifs</term><term>Amino Acid Sequence</term><term>Cell Death</term><term>Gene Deletion</term><term>Gene Silencing</term><term>Molecular Sequence Data</term><term>Plant Immunity</term><term>Protein Structure, Tertiary</term><term>Protein Transport</term><term>Species Specificity</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">• The Phytophthora sojae genome encodes hundreds of RxLR effectors predicted to manipulate various plant defense responses, but the molecular mechanisms involved are largely unknown. Here we have characterized in detail the P. sojae RxLR effector Avh241. • To determine the function and localization of Avh241, we transiently expressed it on different plants. Silencing of Avh241 in P. sojae, we determined its virulence during infection. Through the assay of promoting infection by Phytophthora capsici to Nicotiana benthamiana, we further confirmed this virulence role. • Avh241 induced cell death in several different plants and localized to the plant plasma membrane. An N-terminal motif within Avh241 was important for membrane localization and cell death-inducing activity. Two mitogen-activated protein kinases, NbMEK2 and NbWIPK, were required for the cell death triggered by Avh241 in N. benthamiana. Avh241 was important for the pathogen's full virulence on soybean. Avh241 could also promote infection by P. capsici and the membrane localization motif was not required to promote infection. • This work suggests that Avh241 interacts with the plant immune system via at least two different mechanisms, one recognized by plants dependent on subcellular localization and one promoting infection independent on membrane localization.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22816601</PMID><DateCompleted><Year>2013</Year><Month>01</Month><Day>03</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>196</Volume><Issue>1</Issue><PubDate><Year>2012</Year><Month>Oct</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>The RxLR effector Avh241 from Phytophthora sojae requires plasma membrane localization to induce plant cell death.</ArticleTitle><Pagination><MedlinePgn>247-60</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1469-8137.2012.04241.x</ELocationID><Abstract><AbstractText>• The Phytophthora sojae genome encodes hundreds of RxLR effectors predicted to manipulate various plant defense responses, but the molecular mechanisms involved are largely unknown. Here we have characterized in detail the P. sojae RxLR effector Avh241. • To determine the function and localization of Avh241, we transiently expressed it on different plants. Silencing of Avh241 in P. sojae, we determined its virulence during infection. Through the assay of promoting infection by Phytophthora capsici to Nicotiana benthamiana, we further confirmed this virulence role. • Avh241 induced cell death in several different plants and localized to the plant plasma membrane. An N-terminal motif within Avh241 was important for membrane localization and cell death-inducing activity. Two mitogen-activated protein kinases, NbMEK2 and NbWIPK, were required for the cell death triggered by Avh241 in N. benthamiana. Avh241 was important for the pathogen's full virulence on soybean. Avh241 could also promote infection by P. capsici and the membrane localization motif was not required to promote infection. • This work suggests that Avh241 interacts with the plant immune system via at least two different mechanisms, one recognized by plants dependent on subcellular localization and one promoting infection independent on membrane localization.</AbstractText><CopyrightInformation>© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Xiaoli</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Plant Protection, Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Junli</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Qunqing</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Wenwu</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Tao</LastName><ForeName>Kai</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Duan</LastName><ForeName>Shuyi</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Chenchen</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xinyu</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Suomeng</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Xiaobo</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yuanchao</ForeName><Initials>Y</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>07</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011506">Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.11.24</RegistryNumber><NameOfSubstance UI="D020928">Mitogen-Activated Protein Kinases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020816" MajorTopicYN="N">Amino Acid Motifs</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016923" MajorTopicYN="N">Cell Death</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017353" MajorTopicYN="N">Gene Deletion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020868" MajorTopicYN="N">Gene Silencing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020928" MajorTopicYN="N">Mitogen-Activated Protein Kinases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057865" MajorTopicYN="N">Plant Immunity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017434" MajorTopicYN="N">Protein Structure, Tertiary</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021381" MajorTopicYN="N">Protein Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011506" MajorTopicYN="N">Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013045" MajorTopicYN="N">Species Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013347" MajorTopicYN="N">Subcellular Fractions</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>7</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>7</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>1</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22816601</ArticleId><ArticleId 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