Interaction between abscisic acid and nitric oxide in PB90-induced catharanthine biosynthesis of catharanthus roseus cell suspension cultures.
Identifieur interne : 001301 ( Main/Corpus ); précédent : 001300; suivant : 001302Interaction between abscisic acid and nitric oxide in PB90-induced catharanthine biosynthesis of catharanthus roseus cell suspension cultures.
Auteurs : Qian Chen ; Zunwei Chen ; Li Lu ; Haihong Jin ; Lina Sun ; Qin Yu ; Hongke Xu ; Fengxia Yang ; Mengna Fu ; Shengchao Li ; Huizhong Wang ; Maojun XuSource :
- Biotechnology progress [ 1520-6033 ]
English descriptors
- KwdEn :
- Abscisic Acid (chemistry), Abscisic Acid (metabolism), Catharanthus (cytology), Catharanthus (metabolism), Cell Culture Techniques (MeSH), Cells, Cultured (MeSH), Fungal Proteins (metabolism), Nitric Oxide (chemistry), Nitric Oxide (metabolism), Phytophthora (chemistry), Suspensions (chemistry), Suspensions (metabolism), Vinca Alkaloids (analysis), Vinca Alkaloids (biosynthesis).
- MESH :
- chemical , analysis : Vinca Alkaloids.
- chemical , biosynthesis : Vinca Alkaloids.
- chemical , chemistry : Abscisic Acid, Nitric Oxide, Suspensions.
- chemical , metabolism : Abscisic Acid, Fungal Proteins, Nitric Oxide, Suspensions.
- chemistry : Phytophthora.
- cytology : Catharanthus.
- metabolism : Catharanthus.
- Cell Culture Techniques, Cells, Cultured.
Abstract
Elicitations are considered to be an important strategy to improve production of secondary metabolites of plant cell cultures. However, mechanisms responsible for the elicitor-induced production of secondary metabolites of plant cells have not yet been fully elucidated. Here, we report that treatment of Catharanthus roseus cell suspension cultures with PB90, a protein elicitor from Phytophthora boehmeriae, induced rapid increases of abscisic acid (ABA) and nitric oxide (NO), subsequently followed by the enhancement of catharanthine production and up-regulation of Str and Tdc, two important genes in catharanthine biosynthesis. PB90-induced catharanthine production and the gene expression were suppressed by the ABA inhibitor and NO scavenger respectively, showing that ABA and NO are essential for the elicitor-induced catharanthine biosynthesis. The relationship between ABA and NO in mediating catharanthine biosynthesis was further investigated. Treatment of the cells with ABA triggered NO accumulation and induced catharanthine production and up-regulation of Str and Tdc. ABA-induced catharanthine production and gene expressions were suppressed by the NO scavenger. Conversely, exogenous application of NO did not stimulate ABA generation and treatment with ABA inhibitor did not suppress NO-induced catharanthine production and gene expressions. Together, the results showed that both NO and ABA were involved in PB90-induced catharanthine biosynthesis of C. roseus cells. Furthermore, our data demonstrated that ABA acted upstream of NO in the signaling cascade leading to PB90-induced catharanthine biosynthesis of C. roseus cells.
DOI: 10.1002/btpr.1738
PubMed: 23554409
Links to Exploration step
pubmed:23554409Le document en format XML
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Sun</name></author><author><name sortKey="Yu, Qin" sort="Yu, Qin" uniqKey="Yu Q" first="Qin" last="Yu">Qin Yu</name></author><author><name sortKey="Xu, Hongke" sort="Xu, Hongke" uniqKey="Xu H" first="Hongke" last="Xu">Hongke Xu</name></author><author><name sortKey="Yang, Fengxia" sort="Yang, Fengxia" uniqKey="Yang F" first="Fengxia" last="Yang">Fengxia Yang</name></author><author><name sortKey="Fu, Mengna" sort="Fu, Mengna" uniqKey="Fu M" first="Mengna" last="Fu">Mengna Fu</name></author><author><name sortKey="Li, Shengchao" sort="Li, Shengchao" uniqKey="Li S" first="Shengchao" last="Li">Shengchao Li</name></author><author><name sortKey="Wang, Huizhong" sort="Wang, Huizhong" uniqKey="Wang H" first="Huizhong" last="Wang">Huizhong Wang</name></author><author><name sortKey="Xu, Maojun" sort="Xu, Maojun" uniqKey="Xu M" first="Maojun" last="Xu">Maojun Xu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013 Jul-Aug</date><idno 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L" first="Li" last="Lu">Li Lu</name></author><author><name sortKey="Jin, Haihong" sort="Jin, Haihong" uniqKey="Jin H" first="Haihong" last="Jin">Haihong Jin</name></author><author><name sortKey="Sun, Lina" sort="Sun, Lina" uniqKey="Sun L" first="Lina" last="Sun">Lina Sun</name></author><author><name sortKey="Yu, Qin" sort="Yu, Qin" uniqKey="Yu Q" first="Qin" last="Yu">Qin Yu</name></author><author><name sortKey="Xu, Hongke" sort="Xu, Hongke" uniqKey="Xu H" first="Hongke" last="Xu">Hongke Xu</name></author><author><name sortKey="Yang, Fengxia" sort="Yang, Fengxia" uniqKey="Yang F" first="Fengxia" last="Yang">Fengxia Yang</name></author><author><name sortKey="Fu, Mengna" sort="Fu, Mengna" uniqKey="Fu M" first="Mengna" last="Fu">Mengna Fu</name></author><author><name sortKey="Li, Shengchao" sort="Li, Shengchao" uniqKey="Li S" first="Shengchao" last="Li">Shengchao Li</name></author><author><name sortKey="Wang, Huizhong" sort="Wang, Huizhong" uniqKey="Wang H" first="Huizhong" last="Wang">Huizhong Wang</name></author><author><name sortKey="Xu, Maojun" sort="Xu, Maojun" uniqKey="Xu M" first="Maojun" last="Xu">Maojun Xu</name></author></analytic><series><title level="j">Biotechnology progress</title><idno type="eISSN">1520-6033</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abscisic Acid (chemistry)</term><term>Abscisic Acid (metabolism)</term><term>Catharanthus (cytology)</term><term>Catharanthus (metabolism)</term><term>Cell Culture Techniques (MeSH)</term><term>Cells, Cultured (MeSH)</term><term>Fungal Proteins (metabolism)</term><term>Nitric Oxide (chemistry)</term><term>Nitric Oxide (metabolism)</term><term>Phytophthora (chemistry)</term><term>Suspensions (chemistry)</term><term>Suspensions (metabolism)</term><term>Vinca Alkaloids (analysis)</term><term>Vinca Alkaloids (biosynthesis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" 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xml:lang="en">Elicitations are considered to be an important strategy to improve production of secondary metabolites of plant cell cultures. However, mechanisms responsible for the elicitor-induced production of secondary metabolites of plant cells have not yet been fully elucidated. Here, we report that treatment of Catharanthus roseus cell suspension cultures with PB90, a protein elicitor from Phytophthora boehmeriae, induced rapid increases of abscisic acid (ABA) and nitric oxide (NO), subsequently followed by the enhancement of catharanthine production and up-regulation of Str and Tdc, two important genes in catharanthine biosynthesis. PB90-induced catharanthine production and the gene expression were suppressed by the ABA inhibitor and NO scavenger respectively, showing that ABA and NO are essential for the elicitor-induced catharanthine biosynthesis. The relationship between ABA and NO in mediating catharanthine biosynthesis was further investigated. Treatment of the cells with ABA triggered NO accumulation and induced catharanthine production and up-regulation of Str and Tdc. ABA-induced catharanthine production and gene expressions were suppressed by the NO scavenger. Conversely, exogenous application of NO did not stimulate ABA generation and treatment with ABA inhibitor did not suppress NO-induced catharanthine production and gene expressions. Together, the results showed that both NO and ABA were involved in PB90-induced catharanthine biosynthesis of C. roseus cells. Furthermore, our data demonstrated that ABA acted upstream of NO in the signaling cascade leading to PB90-induced catharanthine biosynthesis of C. roseus cells.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23554409</PMID><DateCompleted><Year>2014</Year><Month>05</Month><Day>14</Day></DateCompleted><DateRevised><Year>2013</Year><Month>08</Month><Day>12</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-6033</ISSN><JournalIssue CitedMedium="Internet"><Volume>29</Volume><Issue>4</Issue><PubDate><MedlineDate>2013 Jul-Aug</MedlineDate></PubDate></JournalIssue><Title>Biotechnology progress</Title><ISOAbbreviation>Biotechnol Prog</ISOAbbreviation></Journal><ArticleTitle>Interaction between abscisic acid and nitric oxide in PB90-induced catharanthine biosynthesis of catharanthus roseus cell suspension cultures.</ArticleTitle><Pagination><MedlinePgn>994-1001</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/btpr.1738</ELocationID><Abstract><AbstractText>Elicitations are considered to be an important strategy to improve production of secondary metabolites of plant cell cultures. However, mechanisms responsible for the elicitor-induced production of secondary metabolites of plant cells have not yet been fully elucidated. Here, we report that treatment of Catharanthus roseus cell suspension cultures with PB90, a protein elicitor from Phytophthora boehmeriae, induced rapid increases of abscisic acid (ABA) and nitric oxide (NO), subsequently followed by the enhancement of catharanthine production and up-regulation of Str and Tdc, two important genes in catharanthine biosynthesis. PB90-induced catharanthine production and the gene expression were suppressed by the ABA inhibitor and NO scavenger respectively, showing that ABA and NO are essential for the elicitor-induced catharanthine biosynthesis. The relationship between ABA and NO in mediating catharanthine biosynthesis was further investigated. Treatment of the cells with ABA triggered NO accumulation and induced catharanthine production and up-regulation of Str and Tdc. ABA-induced catharanthine production and gene expressions were suppressed by the NO scavenger. Conversely, exogenous application of NO did not stimulate ABA generation and treatment with ABA inhibitor did not suppress NO-induced catharanthine production and gene expressions. Together, the results showed that both NO and ABA were involved in PB90-induced catharanthine biosynthesis of C. roseus cells. Furthermore, our data demonstrated that ABA acted upstream of NO in the signaling cascade leading to PB90-induced catharanthine biosynthesis of C. roseus cells.</AbstractText><CopyrightInformation>© 2013 American Institute of Chemical Engineers.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Qian</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medical Plants, Hangzhou Normal University, Hangzhou, 310035, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Zunwei</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Li</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Haihong</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Lina</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Qin</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Hongke</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Fengxia</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Fu</LastName><ForeName>Mengna</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Shengchao</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Huizhong</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Maojun</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>05</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Prog</MedlineTA><NlmUniqueID>8506292</NlmUniqueID><ISSNLinking>1520-6033</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013535">Suspensions</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014748">Vinca Alkaloids</NameOfSubstance></Chemical><Chemical><RegistryNumber>31C4KY9ESH</RegistryNumber><NameOfSubstance UI="D009569">Nitric Oxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>72S9A8J5GW</RegistryNumber><NameOfSubstance UI="D000040">Abscisic 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Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009569" MajorTopicYN="N">Nitric Oxide</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013535" MajorTopicYN="N">Suspensions</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014748" MajorTopicYN="N">Vinca Alkaloids</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Catharanthus roseus cell suspension culture</Keyword><Keyword MajorTopicYN="N">PB90</Keyword><Keyword MajorTopicYN="N">abscisic acid (ABA)</Keyword><Keyword MajorTopicYN="N">catharanthine</Keyword><Keyword MajorTopicYN="N">nitric oxide (NO)</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>07</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>03</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>4</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>4</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>5</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23554409</ArticleId><ArticleId IdType="doi">10.1002/btpr.1738</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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