PsMPK1, an SLT2-type mitogen-activated protein kinase, is required for hyphal growth, zoosporogenesis, cell wall integrity, and pathogenicity in Phytophthora sojae.
Identifieur interne : 001138 ( Main/Corpus ); précédent : 001137; suivant : 001139PsMPK1, an SLT2-type mitogen-activated protein kinase, is required for hyphal growth, zoosporogenesis, cell wall integrity, and pathogenicity in Phytophthora sojae.
Auteurs : Aining Li ; Meng Zhang ; Yonglin Wang ; Delong Li ; Xiaoyun Liu ; Kai Tao ; Wenwu Ye ; Yuanchao WangSource :
- Fungal genetics and biology : FG & B [ 1096-0937 ] ; 2014.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Cell Wall (physiology), Hyphae (growth & development), Hyphae (physiology), Mitogen-Activated Protein Kinases (metabolism), Molecular Sequence Data (MeSH), Mutation (MeSH), Phenotype (MeSH), Phytophthora (pathogenicity), Phytophthora (physiology), Soybeans (microbiology), Spores, Fungal (growth & development), Spores, Fungal (physiology).
- MESH :
- chemical , metabolism : Mitogen-Activated Protein Kinases.
- growth & development : Hyphae, Spores, Fungal.
- microbiology : Soybeans.
- pathogenicity : Phytophthora.
- physiology : Cell Wall, Hyphae, Phytophthora, Spores, Fungal.
- Amino Acid Sequence, Molecular Sequence Data, Mutation, Phenotype.
Abstract
Mitogen-activated protein kinases (MAPKs) play important roles in the regulation of vegetative and pathogenic growth in plant pathogens. Here, we identified an SLT2-type MAP kinase in Phytophthora sojae, PsMPK1, which was transcriptionally induced in sporulating hyphae and the early stages of infection. Silencing of PsMPK1 caused defects in growth and zoosporogenesis, and increased hyphal swellings after the induction of sporangia formation, along with increasing hypersensitivity to cell wall-degrading enzymes. Transmission electron microscopy showed that the cell wall of PsMPK1-silenced mutants was also deleteriously affected. A dark outermost layer in the cell walls disappeared in the mutants, and an additional layer of the mutant cell wall that was deposited abnormally inside an inner bright layer appeared nonhomogeneous and rough compared to the wild type. Pathogenicity assays showed that PsMPK1-silenced transformants lost their pathogenicity on susceptible soybean host plants and triggered stronger cell death. Overall, PsMPK1 is involved in growth, differentiation, cell wall integrity, and pathogenicity in P. sojae.
DOI: 10.1016/j.fgb.2014.01.003
PubMed: 24480463
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pubmed:24480463Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">PsMPK1, an SLT2-type mitogen-activated protein kinase, is required for hyphal growth, zoosporogenesis, cell wall integrity, and pathogenicity in Phytophthora sojae.</title><author><name sortKey="Li, Aining" sort="Li, Aining" uniqKey="Li A" first="Aining" last="Li">Aining Li</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Meng" sort="Zhang, Meng" uniqKey="Zhang M" first="Meng" last="Zhang">Meng Zhang</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Yonglin" sort="Wang, Yonglin" uniqKey="Wang Y" first="Yonglin" last="Wang">Yonglin Wang</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Delong" sort="Li, Delong" uniqKey="Li D" first="Delong" last="Li">Delong Li</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Xiaoyun" sort="Liu, Xiaoyun" uniqKey="Liu X" first="Xiaoyun" last="Liu">Xiaoyun Liu</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tao, Kai" sort="Tao, Kai" uniqKey="Tao K" first="Kai" last="Tao">Kai Tao</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ye, Wenwu" sort="Ye, Wenwu" uniqKey="Ye W" first="Wenwu" last="Ye">Wenwu Ye</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wangyc@njau.edu.cn.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24480463</idno><idno type="pmid">24480463</idno><idno type="doi">10.1016/j.fgb.2014.01.003</idno><idno type="wicri:Area/Main/Corpus">001138</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001138</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">PsMPK1, an SLT2-type mitogen-activated protein kinase, is required for hyphal growth, zoosporogenesis, cell wall integrity, and pathogenicity in Phytophthora sojae.</title><author><name sortKey="Li, Aining" sort="Li, Aining" uniqKey="Li A" first="Aining" last="Li">Aining Li</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Meng" sort="Zhang, Meng" uniqKey="Zhang M" first="Meng" last="Zhang">Meng Zhang</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Yonglin" sort="Wang, Yonglin" uniqKey="Wang Y" first="Yonglin" last="Wang">Yonglin Wang</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Delong" sort="Li, Delong" uniqKey="Li D" first="Delong" last="Li">Delong Li</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, Xiaoyun" sort="Liu, Xiaoyun" uniqKey="Liu X" first="Xiaoyun" last="Liu">Xiaoyun Liu</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tao, Kai" sort="Tao, Kai" uniqKey="Tao K" first="Kai" last="Tao">Kai Tao</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ye, Wenwu" sort="Ye, Wenwu" uniqKey="Ye W" first="Wenwu" last="Ye">Wenwu Ye</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name><affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wangyc@njau.edu.cn.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Fungal genetics and biology : FG & B</title><idno type="eISSN">1096-0937</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Cell Wall (physiology)</term><term>Hyphae (growth & development)</term><term>Hyphae (physiology)</term><term>Mitogen-Activated Protein Kinases (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Mutation (MeSH)</term><term>Phenotype (MeSH)</term><term>Phytophthora (pathogenicity)</term><term>Phytophthora (physiology)</term><term>Soybeans (microbiology)</term><term>Spores, Fungal (growth & development)</term><term>Spores, Fungal (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mitogen-Activated Protein Kinases</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Hyphae</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Wall</term><term>Hyphae</term><term>Phytophthora</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Phenotype</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mitogen-activated protein kinases (MAPKs) play important roles in the regulation of vegetative and pathogenic growth in plant pathogens. Here, we identified an SLT2-type MAP kinase in Phytophthora sojae, PsMPK1, which was transcriptionally induced in sporulating hyphae and the early stages of infection. Silencing of PsMPK1 caused defects in growth and zoosporogenesis, and increased hyphal swellings after the induction of sporangia formation, along with increasing hypersensitivity to cell wall-degrading enzymes. Transmission electron microscopy showed that the cell wall of PsMPK1-silenced mutants was also deleteriously affected. A dark outermost layer in the cell walls disappeared in the mutants, and an additional layer of the mutant cell wall that was deposited abnormally inside an inner bright layer appeared nonhomogeneous and rough compared to the wild type. Pathogenicity assays showed that PsMPK1-silenced transformants lost their pathogenicity on susceptible soybean host plants and triggered stronger cell death. Overall, PsMPK1 is involved in growth, differentiation, cell wall integrity, and pathogenicity in P. sojae. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24480463</PMID><DateCompleted><Year>2015</Year><Month>01</Month><Day>17</Day></DateCompleted><DateRevised><Year>2014</Year><Month>03</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0937</ISSN><JournalIssue CitedMedium="Internet"><Volume>65</Volume><PubDate><Year>2014</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Fungal genetics and biology : FG & B</Title><ISOAbbreviation>Fungal Genet Biol</ISOAbbreviation></Journal><ArticleTitle>PsMPK1, an SLT2-type mitogen-activated protein kinase, is required for hyphal growth, zoosporogenesis, cell wall integrity, and pathogenicity in Phytophthora sojae.</ArticleTitle><Pagination><MedlinePgn>14-24</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.fgb.2014.01.003</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1087-1845(14)00005-X</ELocationID><Abstract><AbstractText>Mitogen-activated protein kinases (MAPKs) play important roles in the regulation of vegetative and pathogenic growth in plant pathogens. Here, we identified an SLT2-type MAP kinase in Phytophthora sojae, PsMPK1, which was transcriptionally induced in sporulating hyphae and the early stages of infection. Silencing of PsMPK1 caused defects in growth and zoosporogenesis, and increased hyphal swellings after the induction of sporangia formation, along with increasing hypersensitivity to cell wall-degrading enzymes. Transmission electron microscopy showed that the cell wall of PsMPK1-silenced mutants was also deleteriously affected. A dark outermost layer in the cell walls disappeared in the mutants, and an additional layer of the mutant cell wall that was deposited abnormally inside an inner bright layer appeared nonhomogeneous and rough compared to the wild type. Pathogenicity assays showed that PsMPK1-silenced transformants lost their pathogenicity on susceptible soybean host plants and triggered stronger cell death. Overall, PsMPK1 is involved in growth, differentiation, cell wall integrity, and pathogenicity in P. sojae. </AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Aining</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Meng</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yonglin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Delong</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Xiaoyun</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tao</LastName><ForeName>Kai</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Wenwu</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yuanchao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wangyc@njau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>01</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Fungal Genet Biol</MedlineTA><NlmUniqueID>9607601</NlmUniqueID><ISSNLinking>1087-1845</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 2.7.11.24</RegistryNumber><NameOfSubstance UI="D020928">Mitogen-Activated Protein Kinases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020928" MajorTopicYN="N">Mitogen-Activated Protein Kinases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013172" MajorTopicYN="N">Spores, Fungal</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cell wall integrity</Keyword><Keyword MajorTopicYN="N">MAP kinase</Keyword><Keyword MajorTopicYN="N">Pathogenicity</Keyword><Keyword MajorTopicYN="N">Phytophthora sojae</Keyword><Keyword MajorTopicYN="N">Zoosporogenesis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>07</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>01</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>01</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>2</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>2</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>1</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24480463</ArticleId><ArticleId IdType="pii">S1087-1845(14)00005-X</ArticleId><ArticleId IdType="doi">10.1016/j.fgb.2014.01.003</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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