PsMPK1, an SLT2-type mitogen-activated protein kinase, is required for hyphal growth, zoosporogenesis, cell wall integrity, and pathogenicity in Phytophthora sojae.
Identifieur interne : 001138 ( Main/Corpus ); précédent : 001137; suivant : 001139PsMPK1, an SLT2-type mitogen-activated protein kinase, is required for hyphal growth, zoosporogenesis, cell wall integrity, and pathogenicity in Phytophthora sojae.
Auteurs : Aining Li ; Meng Zhang ; Yonglin Wang ; Delong Li ; Xiaoyun Liu ; Kai Tao ; Wenwu Ye ; Yuanchao WangSource :
- Fungal genetics and biology : FG & B [ 1096-0937 ] ; 2014.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Cell Wall (physiology), Hyphae (growth & development), Hyphae (physiology), Mitogen-Activated Protein Kinases (metabolism), Molecular Sequence Data (MeSH), Mutation (MeSH), Phenotype (MeSH), Phytophthora (pathogenicity), Phytophthora (physiology), Soybeans (microbiology), Spores, Fungal (growth & development), Spores, Fungal (physiology).
- MESH :
- chemical , metabolism : Mitogen-Activated Protein Kinases.
- growth & development : Hyphae, Spores, Fungal.
- microbiology : Soybeans.
- pathogenicity : Phytophthora.
- physiology : Cell Wall, Hyphae, Phytophthora, Spores, Fungal.
- Amino Acid Sequence, Molecular Sequence Data, Mutation, Phenotype.
Abstract
Mitogen-activated protein kinases (MAPKs) play important roles in the regulation of vegetative and pathogenic growth in plant pathogens. Here, we identified an SLT2-type MAP kinase in Phytophthora sojae, PsMPK1, which was transcriptionally induced in sporulating hyphae and the early stages of infection. Silencing of PsMPK1 caused defects in growth and zoosporogenesis, and increased hyphal swellings after the induction of sporangia formation, along with increasing hypersensitivity to cell wall-degrading enzymes. Transmission electron microscopy showed that the cell wall of PsMPK1-silenced mutants was also deleteriously affected. A dark outermost layer in the cell walls disappeared in the mutants, and an additional layer of the mutant cell wall that was deposited abnormally inside an inner bright layer appeared nonhomogeneous and rough compared to the wild type. Pathogenicity assays showed that PsMPK1-silenced transformants lost their pathogenicity on susceptible soybean host plants and triggered stronger cell death. Overall, PsMPK1 is involved in growth, differentiation, cell wall integrity, and pathogenicity in P. sojae.
DOI: 10.1016/j.fgb.2014.01.003
PubMed: 24480463
Links to Exploration step
pubmed:24480463Le document en format XML
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<author><name sortKey="Li, Aining" sort="Li, Aining" uniqKey="Li A" first="Aining" last="Li">Aining Li</name>
<affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation>
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<author><name sortKey="Zhang, Meng" sort="Zhang, Meng" uniqKey="Zhang M" first="Meng" last="Zhang">Meng Zhang</name>
<affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation>
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<author><name sortKey="Wang, Yonglin" sort="Wang, Yonglin" uniqKey="Wang Y" first="Yonglin" last="Wang">Yonglin Wang</name>
<affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation>
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<author><name sortKey="Li, Delong" sort="Li, Delong" uniqKey="Li D" first="Delong" last="Li">Delong Li</name>
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<author><name sortKey="Liu, Xiaoyun" sort="Liu, Xiaoyun" uniqKey="Liu X" first="Xiaoyun" last="Liu">Xiaoyun Liu</name>
<affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation>
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<author><name sortKey="Tao, Kai" sort="Tao, Kai" uniqKey="Tao K" first="Kai" last="Tao">Kai Tao</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">PsMPK1, an SLT2-type mitogen-activated protein kinase, is required for hyphal growth, zoosporogenesis, cell wall integrity, and pathogenicity in Phytophthora sojae.</title>
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<author><name sortKey="Zhang, Meng" sort="Zhang, Meng" uniqKey="Zhang M" first="Meng" last="Zhang">Meng Zhang</name>
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<author><name sortKey="Li, Delong" sort="Li, Delong" uniqKey="Li D" first="Delong" last="Li">Delong Li</name>
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<author><name sortKey="Liu, Xiaoyun" sort="Liu, Xiaoyun" uniqKey="Liu X" first="Xiaoyun" last="Liu">Xiaoyun Liu</name>
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<author><name sortKey="Tao, Kai" sort="Tao, Kai" uniqKey="Tao K" first="Kai" last="Tao">Kai Tao</name>
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<author><name sortKey="Ye, Wenwu" sort="Ye, Wenwu" uniqKey="Ye W" first="Wenwu" last="Ye">Wenwu Ye</name>
<affiliation><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation>
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<author><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name>
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<term>Hyphae (growth & development)</term>
<term>Hyphae (physiology)</term>
<term>Mitogen-Activated Protein Kinases (metabolism)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Mutation (MeSH)</term>
<term>Phenotype (MeSH)</term>
<term>Phytophthora (pathogenicity)</term>
<term>Phytophthora (physiology)</term>
<term>Soybeans (microbiology)</term>
<term>Spores, Fungal (growth & development)</term>
<term>Spores, Fungal (physiology)</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mitogen-Activated Protein Kinases</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Hyphae</term>
<term>Spores, Fungal</term>
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<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Soybeans</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Wall</term>
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<term>Molecular Sequence Data</term>
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<front><div type="abstract" xml:lang="en">Mitogen-activated protein kinases (MAPKs) play important roles in the regulation of vegetative and pathogenic growth in plant pathogens. Here, we identified an SLT2-type MAP kinase in Phytophthora sojae, PsMPK1, which was transcriptionally induced in sporulating hyphae and the early stages of infection. Silencing of PsMPK1 caused defects in growth and zoosporogenesis, and increased hyphal swellings after the induction of sporangia formation, along with increasing hypersensitivity to cell wall-degrading enzymes. Transmission electron microscopy showed that the cell wall of PsMPK1-silenced mutants was also deleteriously affected. A dark outermost layer in the cell walls disappeared in the mutants, and an additional layer of the mutant cell wall that was deposited abnormally inside an inner bright layer appeared nonhomogeneous and rough compared to the wild type. Pathogenicity assays showed that PsMPK1-silenced transformants lost their pathogenicity on susceptible soybean host plants and triggered stronger cell death. Overall, PsMPK1 is involved in growth, differentiation, cell wall integrity, and pathogenicity in P. sojae. </div>
</front>
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<ArticleTitle>PsMPK1, an SLT2-type mitogen-activated protein kinase, is required for hyphal growth, zoosporogenesis, cell wall integrity, and pathogenicity in Phytophthora sojae.</ArticleTitle>
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<Abstract><AbstractText>Mitogen-activated protein kinases (MAPKs) play important roles in the regulation of vegetative and pathogenic growth in plant pathogens. Here, we identified an SLT2-type MAP kinase in Phytophthora sojae, PsMPK1, which was transcriptionally induced in sporulating hyphae and the early stages of infection. Silencing of PsMPK1 caused defects in growth and zoosporogenesis, and increased hyphal swellings after the induction of sporangia formation, along with increasing hypersensitivity to cell wall-degrading enzymes. Transmission electron microscopy showed that the cell wall of PsMPK1-silenced mutants was also deleteriously affected. A dark outermost layer in the cell walls disappeared in the mutants, and an additional layer of the mutant cell wall that was deposited abnormally inside an inner bright layer appeared nonhomogeneous and rough compared to the wild type. Pathogenicity assays showed that PsMPK1-silenced transformants lost their pathogenicity on susceptible soybean host plants and triggered stronger cell death. Overall, PsMPK1 is involved in growth, differentiation, cell wall integrity, and pathogenicity in P. sojae. </AbstractText>
<CopyrightInformation>Copyright © 2014 Elsevier Inc. All rights reserved.</CopyrightInformation>
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