Molecular cloning, functional characterization and expression of potato (Solanum tuberosum) 1-deoxy-d-xylulose 5-phosphate synthase 1 (StDXS1) in response to Phytophthora infestans.
Identifieur interne : 000C88 ( Main/Corpus ); précédent : 000C87; suivant : 000C89Molecular cloning, functional characterization and expression of potato (Solanum tuberosum) 1-deoxy-d-xylulose 5-phosphate synthase 1 (StDXS1) in response to Phytophthora infestans.
Auteurs : Maria Antonia Henriquez ; Atta Soliman ; Genyi Li ; Abdelali Hannoufa ; Belay T. Ayele ; Fouad DaayfSource :
- Plant science : an international journal of experimental plant biology [ 1873-2259 ] ; 2016.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Arabidopsis (metabolism), Arabidopsis (microbiology), Cloning, Molecular (MeSH), DNA, Complementary (genetics), DNA, Complementary (metabolism), DNA, Plant (genetics), DNA, Plant (metabolism), Eicosapentaenoic Acid (metabolism), Gene Expression Regulation, Plant (MeSH), Glucans (metabolism), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Phytophthora infestans (physiology), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Plants, Genetically Modified (microbiology), Sequence Analysis, DNA (MeSH), Transferases (genetics), Transferases (metabolism).
- MESH :
- chemical , genetics : DNA, Complementary, DNA, Plant, Plant Proteins, Transferases.
- genetics : Arabidopsis, Plant Diseases, Plants, Genetically Modified.
- metabolism : Arabidopsis, DNA, Complementary, DNA, Plant, Eicosapentaenoic Acid, Glucans, Plant Proteins, Plants, Genetically Modified, Transferases.
- microbiology : Arabidopsis, Plant Diseases, Plants, Genetically Modified.
- physiology : Phytophthora infestans.
- Cloning, Molecular, Gene Expression Regulation, Plant, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA.
Abstract
1-Deoxy-D-xylulose 5-phosphate synthase (DXS) catalyzes the initial step of the plastidial 2C-methyl-D-erythritol-4-phosphate (DOXP-MEP) pathway involved in isoprenoid biosynthesis. In this study, we cloned the complete cDNA of potato DXS gene that was designated StDXS1. StDXS1 cDNA encodes for 719 amino acid residues, with MW of 77.8 kDa, and is present in one copy in the potato genome. Phylogenetic analysis and protein sequence alignments assigned StDXS1 to a group with DXS homologues from closely related species and exhibited homodomain identity with known DXS proteins from other plant species. Late blight symptoms occurred in parallel with a reduction in StDXS1 transcript levels, which may be associated with the levels of isoprenoids that contribute to plant protection against pathogens. Subcellular localization indicated that StDXS1 targets the chloroplasts where isoprenoids are synthesized. Arabidopsis expressing StDXS1 showed a higher accumulation of carotenoids and chlorophyll as compared to wild type controls. Lower levels of ABA and GA were detected in the transgenic DXS lines as compared to control plants, which reflected on higher germination rates of the transgenic DXS lines. No changes were detected in JA or SA contents. Selected downstream genes in the DOXP-MEP pathway, especially GGPPS genes, were up-regulated in the transgenic lines.
DOI: 10.1016/j.plantsci.2015.12.001
PubMed: 26795152
Links to Exploration step
pubmed:26795152Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular cloning, functional characterization and expression of potato (Solanum tuberosum) 1-deoxy-d-xylulose 5-phosphate synthase 1 (StDXS1) in response to Phytophthora infestans.</title><author><name sortKey="Henriquez, Maria Antonia" sort="Henriquez, Maria Antonia" uniqKey="Henriquez M" first="Maria Antonia" last="Henriquez">Maria Antonia Henriquez</name><affiliation><nlm:affiliation>Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada; Agriculture and Agri-Food Canada, Morden, MB, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Soliman, Atta" sort="Soliman, Atta" uniqKey="Soliman A" first="Atta" last="Soliman">Atta Soliman</name><affiliation><nlm:affiliation>Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada; Department of Genetics, Faculty of Agriculture, University of Tanta, Tanta, El-Gharbia, Egypt.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Genyi" sort="Li, Genyi" uniqKey="Li G" first="Genyi" last="Li">Genyi Li</name><affiliation><nlm:affiliation>Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Hannoufa, Abdelali" sort="Hannoufa, Abdelali" uniqKey="Hannoufa A" first="Abdelali" last="Hannoufa">Abdelali Hannoufa</name><affiliation><nlm:affiliation>Agriculture and Agri-Food Canada, London, Ontario, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Ayele, Belay T" sort="Ayele, Belay T" uniqKey="Ayele B" first="Belay T" last="Ayele">Belay T. Ayele</name><affiliation><nlm:affiliation>Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Daayf, Fouad" sort="Daayf, Fouad" uniqKey="Daayf F" first="Fouad" last="Daayf">Fouad Daayf</name><affiliation><nlm:affiliation>Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada. 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Ayele</name><affiliation><nlm:affiliation>Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Daayf, Fouad" sort="Daayf, Fouad" uniqKey="Daayf F" first="Fouad" last="Daayf">Fouad Daayf</name><affiliation><nlm:affiliation>Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada. Electronic address: Fouad.Daayf@umanitoba.ca.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Plant science : an international journal of experimental plant biology</title><idno type="eISSN">1873-2259</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (genetics)</term><term>Arabidopsis (metabolism)</term><term>Arabidopsis (microbiology)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Complementary (genetics)</term><term>DNA, Complementary (metabolism)</term><term>DNA, Plant (genetics)</term><term>DNA, Plant (metabolism)</term><term>Eicosapentaenoic Acid (metabolism)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Glucans (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Phytophthora infestans (physiology)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Plants, Genetically Modified (microbiology)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Transferases (genetics)</term><term>Transferases (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>DNA, Plant</term><term>Plant Proteins</term><term>Transferases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Plant Diseases</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>DNA, Complementary</term><term>DNA, Plant</term><term>Eicosapentaenoic Acid</term><term>Glucans</term><term>Plant Proteins</term><term>Plants, Genetically Modified</term><term>Transferases</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Arabidopsis</term><term>Plant Diseases</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">1-Deoxy-D-xylulose 5-phosphate synthase (DXS) catalyzes the initial step of the plastidial 2C-methyl-D-erythritol-4-phosphate (DOXP-MEP) pathway involved in isoprenoid biosynthesis. In this study, we cloned the complete cDNA of potato DXS gene that was designated StDXS1. StDXS1 cDNA encodes for 719 amino acid residues, with MW of 77.8 kDa, and is present in one copy in the potato genome. Phylogenetic analysis and protein sequence alignments assigned StDXS1 to a group with DXS homologues from closely related species and exhibited homodomain identity with known DXS proteins from other plant species. Late blight symptoms occurred in parallel with a reduction in StDXS1 transcript levels, which may be associated with the levels of isoprenoids that contribute to plant protection against pathogens. Subcellular localization indicated that StDXS1 targets the chloroplasts where isoprenoids are synthesized. Arabidopsis expressing StDXS1 showed a higher accumulation of carotenoids and chlorophyll as compared to wild type controls. Lower levels of ABA and GA were detected in the transgenic DXS lines as compared to control plants, which reflected on higher germination rates of the transgenic DXS lines. No changes were detected in JA or SA contents. Selected downstream genes in the DOXP-MEP pathway, especially GGPPS genes, were up-regulated in the transgenic lines.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26795152</PMID><DateCompleted><Year>2016</Year><Month>10</Month><Day>19</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2259</ISSN><JournalIssue CitedMedium="Internet"><Volume>243</Volume><PubDate><Year>2016</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Plant science : an international journal of experimental plant biology</Title><ISOAbbreviation>Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Molecular cloning, functional characterization and expression of potato (Solanum tuberosum) 1-deoxy-d-xylulose 5-phosphate synthase 1 (StDXS1) in response to Phytophthora infestans.</ArticleTitle><Pagination><MedlinePgn>71-83</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plantsci.2015.12.001</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0168-9452(15)30111-4</ELocationID><Abstract><AbstractText>1-Deoxy-D-xylulose 5-phosphate synthase (DXS) catalyzes the initial step of the plastidial 2C-methyl-D-erythritol-4-phosphate (DOXP-MEP) pathway involved in isoprenoid biosynthesis. In this study, we cloned the complete cDNA of potato DXS gene that was designated StDXS1. StDXS1 cDNA encodes for 719 amino acid residues, with MW of 77.8 kDa, and is present in one copy in the potato genome. Phylogenetic analysis and protein sequence alignments assigned StDXS1 to a group with DXS homologues from closely related species and exhibited homodomain identity with known DXS proteins from other plant species. Late blight symptoms occurred in parallel with a reduction in StDXS1 transcript levels, which may be associated with the levels of isoprenoids that contribute to plant protection against pathogens. Subcellular localization indicated that StDXS1 targets the chloroplasts where isoprenoids are synthesized. Arabidopsis expressing StDXS1 showed a higher accumulation of carotenoids and chlorophyll as compared to wild type controls. Lower levels of ABA and GA were detected in the transgenic DXS lines as compared to control plants, which reflected on higher germination rates of the transgenic DXS lines. No changes were detected in JA or SA contents. Selected downstream genes in the DOXP-MEP pathway, especially GGPPS genes, were up-regulated in the transgenic lines.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Henriquez</LastName><ForeName>Maria Antonia</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada; Agriculture and Agri-Food Canada, Morden, MB, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Soliman</LastName><ForeName>Atta</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada; Department of Genetics, Faculty of Agriculture, University of Tanta, Tanta, El-Gharbia, Egypt.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Genyi</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hannoufa</LastName><ForeName>Abdelali</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Agriculture and Agri-Food Canada, London, Ontario, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ayele</LastName><ForeName>Belay T</ForeName><Initials>BT</Initials><AffiliationInfo><Affiliation>Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Daayf</LastName><ForeName>Fouad</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Plant Science, University of Manitoba, 222 Agriculture Building, Winnipeg, MB R3T2N2, Canada. Electronic address: Fouad.Daayf@umanitoba.ca.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>12</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Ireland</Country><MedlineTA>Plant Sci</MedlineTA><NlmUniqueID>9882015</NlmUniqueID><ISSNLinking>0168-9452</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015118" MajorTopicYN="N">Eicosapentaenoic Acid</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005936" MajorTopicYN="N">Glucans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014166" MajorTopicYN="N">Transferases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Abscisic acid</Keyword><Keyword MajorTopicYN="N">Carotenoids</Keyword><Keyword MajorTopicYN="N">DOXP-MEP</Keyword><Keyword MajorTopicYN="N">DXS</Keyword><Keyword MajorTopicYN="N">Functional analysis</Keyword><Keyword MajorTopicYN="N">Jasmonic acid</Keyword><Keyword MajorTopicYN="N">Phytophthora infestans</Keyword><Keyword MajorTopicYN="N">Salicylic acid</Keyword><Keyword MajorTopicYN="N">Solanum</Keyword><Keyword MajorTopicYN="N">Terpenoids</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>07</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>10</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>12</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>1</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>1</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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