Small RNA and mRNA Profiling of Arabidopsis in Response to Phytophthora Infection and PAMP Treatment.
Identifieur interne : 000A33 ( Main/Corpus ); précédent : 000A32; suivant : 000A34Small RNA and mRNA Profiling of Arabidopsis in Response to Phytophthora Infection and PAMP Treatment.
Auteurs : Yingnan Hou ; Wenbo MaSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2017.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Arabidopsis (immunology), Arabidopsis (parasitology), Gene Expression Profiling (methods), Gene Expression Regulation, Plant (MeSH), Gene Regulatory Networks (MeSH), Pathogen-Associated Molecular Pattern Molecules (immunology), Phytophthora (pathogenicity), Plant Diseases (genetics), Plant Diseases (parasitology), Plant Immunity (MeSH), RNA, Messenger (genetics), RNA, Plant (genetics), RNA, Small Untranslated (genetics), Sequence Analysis, RNA (methods).
- MESH :
- chemical , genetics : RNA, Messenger, RNA, Plant, RNA, Small Untranslated.
- chemical , immunology : Pathogen-Associated Molecular Pattern Molecules.
- genetics : Arabidopsis, Plant Diseases.
- immunology : Arabidopsis.
- methods : Gene Expression Profiling, Sequence Analysis, RNA.
- parasitology : Arabidopsis, Plant Diseases.
- pathogenicity : Phytophthora.
- Gene Expression Regulation, Plant, Gene Regulatory Networks, Plant Immunity.
Abstract
Small non-coding RNAs (smRNAs) regulate gene expression at both transcriptional and post-transcriptional levels. Well known for their roles in development, smRNAs have emerged as important regulators of plant immunity. Upon pathogen perception, accumulation of specific smRNAs are found to be altered, presumably as a host defense response. Therefore, identification of differentially accumulated smRNAs and their target genes would provide important insight into the regulation mechanism of immune responses. Here, we describe the detailed experimental procedure using Illumina sequencing to analyze the expression profiles of smRNAs and mRNAs in Arabidopsis. We focus on a newly developed pathosystem using Phytophthora capsici as the pathogen and include the treatment of Arabidopsis leaves with pathogen-associated molecular patterns (PAMPs) of Phytophthora.
DOI: 10.1007/978-1-4939-6859-6_23
PubMed: 28220433
Links to Exploration step
pubmed:28220433Le document en format XML
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<author><name sortKey="Hou, Yingnan" sort="Hou, Yingnan" uniqKey="Hou Y" first="Yingnan" last="Hou">Yingnan Hou</name>
<affiliation><nlm:affiliation>Department of Plant Pathology and Microbiology, University of California, Riverside, CA, 92521, USA.</nlm:affiliation>
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<affiliation><nlm:affiliation>Center for Plant Cell Biology, University of California, Riverside, CA, 92521, USA.</nlm:affiliation>
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<author><name sortKey="Ma, Wenbo" sort="Ma, Wenbo" uniqKey="Ma W" first="Wenbo" last="Ma">Wenbo Ma</name>
<affiliation><nlm:affiliation>Department of Plant Pathology and Microbiology, University of California, Riverside, CA, 92521, USA. wenbo.ma@ucr.edu.</nlm:affiliation>
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<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Gene Regulatory Networks (MeSH)</term>
<term>Pathogen-Associated Molecular Pattern Molecules (immunology)</term>
<term>Phytophthora (pathogenicity)</term>
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<front><div type="abstract" xml:lang="en">Small non-coding RNAs (smRNAs) regulate gene expression at both transcriptional and post-transcriptional levels. Well known for their roles in development, smRNAs have emerged as important regulators of plant immunity. Upon pathogen perception, accumulation of specific smRNAs are found to be altered, presumably as a host defense response. Therefore, identification of differentially accumulated smRNAs and their target genes would provide important insight into the regulation mechanism of immune responses. Here, we describe the detailed experimental procedure using Illumina sequencing to analyze the expression profiles of smRNAs and mRNAs in Arabidopsis. We focus on a newly developed pathosystem using Phytophthora capsici as the pathogen and include the treatment of Arabidopsis leaves with pathogen-associated molecular patterns (PAMPs) of Phytophthora.</div>
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<Abstract><AbstractText>Small non-coding RNAs (smRNAs) regulate gene expression at both transcriptional and post-transcriptional levels. Well known for their roles in development, smRNAs have emerged as important regulators of plant immunity. Upon pathogen perception, accumulation of specific smRNAs are found to be altered, presumably as a host defense response. Therefore, identification of differentially accumulated smRNAs and their target genes would provide important insight into the regulation mechanism of immune responses. Here, we describe the detailed experimental procedure using Illumina sequencing to analyze the expression profiles of smRNAs and mRNAs in Arabidopsis. We focus on a newly developed pathosystem using Phytophthora capsici as the pathogen and include the treatment of Arabidopsis leaves with pathogen-associated molecular patterns (PAMPs) of Phytophthora.</AbstractText>
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