Small RNA and mRNA Profiling of Arabidopsis in Response to Phytophthora Infection and PAMP Treatment.
Identifieur interne : 000A33 ( Main/Corpus ); précédent : 000A32; suivant : 000A34Small RNA and mRNA Profiling of Arabidopsis in Response to Phytophthora Infection and PAMP Treatment.
Auteurs : Yingnan Hou ; Wenbo MaSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2017.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Arabidopsis (immunology), Arabidopsis (parasitology), Gene Expression Profiling (methods), Gene Expression Regulation, Plant (MeSH), Gene Regulatory Networks (MeSH), Pathogen-Associated Molecular Pattern Molecules (immunology), Phytophthora (pathogenicity), Plant Diseases (genetics), Plant Diseases (parasitology), Plant Immunity (MeSH), RNA, Messenger (genetics), RNA, Plant (genetics), RNA, Small Untranslated (genetics), Sequence Analysis, RNA (methods).
- MESH :
- chemical , genetics : RNA, Messenger, RNA, Plant, RNA, Small Untranslated.
- chemical , immunology : Pathogen-Associated Molecular Pattern Molecules.
- genetics : Arabidopsis, Plant Diseases.
- immunology : Arabidopsis.
- methods : Gene Expression Profiling, Sequence Analysis, RNA.
- parasitology : Arabidopsis, Plant Diseases.
- pathogenicity : Phytophthora.
- Gene Expression Regulation, Plant, Gene Regulatory Networks, Plant Immunity.
Abstract
Small non-coding RNAs (smRNAs) regulate gene expression at both transcriptional and post-transcriptional levels. Well known for their roles in development, smRNAs have emerged as important regulators of plant immunity. Upon pathogen perception, accumulation of specific smRNAs are found to be altered, presumably as a host defense response. Therefore, identification of differentially accumulated smRNAs and their target genes would provide important insight into the regulation mechanism of immune responses. Here, we describe the detailed experimental procedure using Illumina sequencing to analyze the expression profiles of smRNAs and mRNAs in Arabidopsis. We focus on a newly developed pathosystem using Phytophthora capsici as the pathogen and include the treatment of Arabidopsis leaves with pathogen-associated molecular patterns (PAMPs) of Phytophthora.
DOI: 10.1007/978-1-4939-6859-6_23
PubMed: 28220433
Links to Exploration step
pubmed:28220433Le document en format XML
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Well known for their roles in development, smRNAs have emerged as important regulators of plant immunity. Upon pathogen perception, accumulation of specific smRNAs are found to be altered, presumably as a host defense response. Therefore, identification of differentially accumulated smRNAs and their target genes would provide important insight into the regulation mechanism of immune responses. Here, we describe the detailed experimental procedure using Illumina sequencing to analyze the expression profiles of smRNAs and mRNAs in Arabidopsis. We focus on a newly developed pathosystem using Phytophthora capsici as the pathogen and include the treatment of Arabidopsis leaves with pathogen-associated molecular patterns (PAMPs) of Phytophthora.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28220433</PMID><DateCompleted><Year>2018</Year><Month>02</Month><Day>05</Day></DateCompleted><DateRevised><Year>2018</Year><Month>05</Month><Day>03</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1578</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Small RNA and mRNA Profiling of Arabidopsis in Response to Phytophthora Infection and PAMP Treatment.</ArticleTitle><Pagination><MedlinePgn>273-283</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-6859-6_23</ELocationID><Abstract><AbstractText>Small non-coding RNAs (smRNAs) regulate gene expression at both transcriptional and post-transcriptional levels. Well known for their roles in development, smRNAs have emerged as important regulators of plant immunity. Upon pathogen perception, accumulation of specific smRNAs are found to be altered, presumably as a host defense response. Therefore, identification of differentially accumulated smRNAs and their target genes would provide important insight into the regulation mechanism of immune responses. Here, we describe the detailed experimental procedure using Illumina sequencing to analyze the expression profiles of smRNAs and mRNAs in Arabidopsis. 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Molecules</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057865" MajorTopicYN="N">Plant Immunity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058727" MajorTopicYN="N">RNA, Small Untranslated</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017423" MajorTopicYN="N">Sequence Analysis, RNA</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Next-generation sequencing</Keyword><Keyword MajorTopicYN="Y">Pattern-triggered immune response</Keyword><Keyword MajorTopicYN="Y">Phytophthora infection</Keyword><Keyword MajorTopicYN="Y">RNA-Seq</Keyword><Keyword MajorTopicYN="Y">Small RNA-Seq</Keyword><Keyword MajorTopicYN="Y">Small RNA-regulated defense response</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate 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