Sulphated Polysaccharide from Acanthophora spicifera Induced Hevea brasiliensis Defense Responses Against Phytophthora palmivora Infection.
Identifieur interne : 000516 ( Main/Corpus ); précédent : 000515; suivant : 000517Sulphated Polysaccharide from Acanthophora spicifera Induced Hevea brasiliensis Defense Responses Against Phytophthora palmivora Infection.
Auteurs : Sittiporn Pettongkhao ; Abdulmuhaimin Bilanglod ; Khemmikar Khompatara ; Nunta ChurngchowSource :
- Plants (Basel, Switzerland) [ 2223-7747 ] ; 2019.
Abstract
Elicitors from seaweeds are considered an alternative stimulant of plant defenses against pathogenic infection. Finding new sources of elicitors and exploring their effects on plant defenses is a significant undertaking. In this study, we extracted crude polysaccharide (CPS) from Acanthophora spicifera (a red alga) and tested the effects of the compound on rubber tree (Hevea brasiliensis) defense responses. Accumulations of salicylic acid (SA) and scopoletin (Scp) were measured by HPLC. The expression of SA- and Jasmonic acid (JA)-responsive genes was analyzed by semi-qRT-PCR. Strong anion exchange chromatography and Fourier-transform infrared (FTIR) spectroscopy were used for purification and functional characterization of CPS, respectively. The extracted CPS enhanced rubber tree defenses against Phytophthorapalmivora infection. It induced SA and Scp accumulations and SA-responsive gene expression, but suppressed JA-responsive gene expression. We successfully separated the non-sulphated polysaccharide (F1) from the sulphated polysaccharides (SPS). Both peaks of SPS (F2 and F3) were identified as lambda (λ)-carrageenan. The F3 fraction showed greater elicitor activity on tobacco leaves. It induced SA and Scp accumulations and peroxidase activity but suppressed catalase activity. Furthermore, the purified λ-carrageenan did not cause cell death in tobacco or rubber tree leaves. Therefore, the elicitor from A. spicifera could be an alternative plant stimulant.
DOI: 10.3390/plants8030073
PubMed: 30909469
PubMed Central: PMC6473620
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pubmed:30909469Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sulphated Polysaccharide from <i>Acanthophora spicifera</i>
Induced <i>Hevea brasiliensis</i>
Defense Responses Against <i>Phytophthora palmivora</i>
Infection.</title>
<author><name sortKey="Pettongkhao, Sittiporn" sort="Pettongkhao, Sittiporn" uniqKey="Pettongkhao S" first="Sittiporn" last="Pettongkhao">Sittiporn Pettongkhao</name>
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<author><name sortKey="Bilanglod, Abdulmuhaimin" sort="Bilanglod, Abdulmuhaimin" uniqKey="Bilanglod A" first="Abdulmuhaimin" last="Bilanglod">Abdulmuhaimin Bilanglod</name>
<affiliation><nlm:affiliation>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. Ameen094584@hotmail.com.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Khompatara, Khemmikar" sort="Khompatara, Khemmikar" uniqKey="Khompatara K" first="Khemmikar" last="Khompatara">Khemmikar Khompatara</name>
<affiliation><nlm:affiliation>Office of Agricultural Research and Development Region 8, Department of Agriculture, Ministry of Agriculture and Cooperatives, Hat-Yai, Songkhla 90110, Thailand. kjoy2000@hotmail.com.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Churngchow, Nunta" sort="Churngchow, Nunta" uniqKey="Churngchow N" first="Nunta" last="Churngchow">Nunta Churngchow</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Sulphated Polysaccharide from <i>Acanthophora spicifera</i>
Induced <i>Hevea brasiliensis</i>
Defense Responses Against <i>Phytophthora palmivora</i>
Infection.</title>
<author><name sortKey="Pettongkhao, Sittiporn" sort="Pettongkhao, Sittiporn" uniqKey="Pettongkhao S" first="Sittiporn" last="Pettongkhao">Sittiporn Pettongkhao</name>
<affiliation><nlm:affiliation>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. sit_ti_pon@windowslive.com.</nlm:affiliation>
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<author><name sortKey="Bilanglod, Abdulmuhaimin" sort="Bilanglod, Abdulmuhaimin" uniqKey="Bilanglod A" first="Abdulmuhaimin" last="Bilanglod">Abdulmuhaimin Bilanglod</name>
<affiliation><nlm:affiliation>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. Ameen094584@hotmail.com.</nlm:affiliation>
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<author><name sortKey="Khompatara, Khemmikar" sort="Khompatara, Khemmikar" uniqKey="Khompatara K" first="Khemmikar" last="Khompatara">Khemmikar Khompatara</name>
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<author><name sortKey="Churngchow, Nunta" sort="Churngchow, Nunta" uniqKey="Churngchow N" first="Nunta" last="Churngchow">Nunta Churngchow</name>
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<front><div type="abstract" xml:lang="en">Elicitors from seaweeds are considered an alternative stimulant of plant defenses against pathogenic infection. Finding new sources of elicitors and exploring their effects on plant defenses is a significant undertaking. In this study, we extracted crude polysaccharide (CPS) from <i>Acanthophora spicifera</i>
(a red alga) and tested the effects of the compound on rubber tree (<i>Hevea brasiliensis</i>
) defense responses. Accumulations of salicylic acid (SA) and scopoletin (Scp) were measured by HPLC. The expression of SA- and Jasmonic acid (JA)-responsive genes was analyzed by semi-qRT-PCR. Strong anion exchange chromatography and Fourier-transform infrared (FTIR) spectroscopy were used for purification and functional characterization of CPS, respectively. The extracted CPS enhanced rubber tree defenses against <i>Phytophthora</i>
<i>palmivora</i>
infection. It induced SA and Scp accumulations and SA-responsive gene expression, but suppressed JA-responsive gene expression. We successfully separated the non-sulphated polysaccharide (F1) from the sulphated polysaccharides (SPS). Both peaks of SPS (F2 and F3) were identified as lambda (λ)-carrageenan. The F3 fraction showed greater elicitor activity on tobacco leaves. It induced SA and Scp accumulations and peroxidase activity but suppressed catalase activity. Furthermore, the purified λ-carrageenan did not cause cell death in tobacco or rubber tree leaves. Therefore, the elicitor from <i>A. spicifera</i>
could be an alternative plant stimulant.</div>
</front>
</TEI>
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<Month>09</Month>
<Day>29</Day>
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<Title>Plants (Basel, Switzerland)</Title>
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<ArticleTitle>Sulphated Polysaccharide from <i>Acanthophora spicifera</i>
Induced <i>Hevea brasiliensis</i>
Defense Responses Against <i>Phytophthora palmivora</i>
Infection.</ArticleTitle>
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<Abstract><AbstractText>Elicitors from seaweeds are considered an alternative stimulant of plant defenses against pathogenic infection. Finding new sources of elicitors and exploring their effects on plant defenses is a significant undertaking. In this study, we extracted crude polysaccharide (CPS) from <i>Acanthophora spicifera</i>
(a red alga) and tested the effects of the compound on rubber tree (<i>Hevea brasiliensis</i>
) defense responses. Accumulations of salicylic acid (SA) and scopoletin (Scp) were measured by HPLC. The expression of SA- and Jasmonic acid (JA)-responsive genes was analyzed by semi-qRT-PCR. Strong anion exchange chromatography and Fourier-transform infrared (FTIR) spectroscopy were used for purification and functional characterization of CPS, respectively. The extracted CPS enhanced rubber tree defenses against <i>Phytophthora</i>
<i>palmivora</i>
infection. It induced SA and Scp accumulations and SA-responsive gene expression, but suppressed JA-responsive gene expression. We successfully separated the non-sulphated polysaccharide (F1) from the sulphated polysaccharides (SPS). Both peaks of SPS (F2 and F3) were identified as lambda (λ)-carrageenan. The F3 fraction showed greater elicitor activity on tobacco leaves. It induced SA and Scp accumulations and peroxidase activity but suppressed catalase activity. Furthermore, the purified λ-carrageenan did not cause cell death in tobacco or rubber tree leaves. Therefore, the elicitor from <i>A. spicifera</i>
could be an alternative plant stimulant.</AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pettongkhao</LastName>
<ForeName>Sittiporn</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. sit_ti_pon@windowslive.com.</Affiliation>
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<AffiliationInfo><Affiliation>Office of Agricultural Research and Development Region 8, Department of Agriculture, Ministry of Agriculture and Cooperatives, Hat-Yai, Songkhla 90110, Thailand. kjoy2000@hotmail.com.</Affiliation>
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<AffiliationInfo><Affiliation>Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. nunta.c@psu.ac.th.</Affiliation>
</AffiliationInfo>
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<Agency>The Excellent Scholarships in Biochemistry, Department of Biochemistry, Faculty of Science, Prince of Songkla University, Thailand</Agency>
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<ReferenceList><Reference><Citation>Plant Physiol. 2000 Nov;124(3):1027-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11080280</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 2001 May;42(5):462-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11382811</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2003 Feb;16(2):115-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12575745</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Microbiol. 2003 Oct;11(10):462-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14557029</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Carbohydr Res. 2004 Jan 22;339(2):335-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14698892</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2004 Nov;16(11):3020-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15494557</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biotechnol Adv. 2005 Jun;23(4):283-333</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15848039</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 1972 Jun;49(6):950-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16658089</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Indian J Exp Biol. 2006 Jun;44(6):492-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16784121</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochim Biophys Acta. 2007 Sep;1767(9):1073-101</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17692815</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Sci. 2011 Aug;181(2):132-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21683878</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Plant Physiol. 2011 Oct 15;168(15):1784-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21742407</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2011;6(10):e26834</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22046375</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mar Drugs. 2011 Dec;9(12):2514-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22363237</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Cell Dev Biol. 2012;28:489-521</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22559264</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Sci. 2013 Jun;207:79-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23602102</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Pathol. 2015 Feb;16(2):123-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24965864</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol Biochem. 2016 Apr;101:76-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26854410</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2016 Jun 23;11(6):e0157591</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27337148</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Mol Sci. 2018 Jun 26;19(7):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29949940</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Biochem. 1971 Nov;44(1):301-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">4109029</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Biol Macromol. 1995 Apr;17(2):74-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7547718</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1993 Dec 17;262(5141):1883-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8266079</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9533-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8415736</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1996 Aug;8(8):1225-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8776893</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 1997 Dec;10(9):1045-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9390419</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1998 Sep;10(9):1571-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9724702</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
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