Lignin peroxidase-negative mutant of the white-rot basidiomycete Phanerochaete chrysosporium.
Identifieur interne : 000F49 ( Main/Exploration ); précédent : 000F48; suivant : 000F50Lignin peroxidase-negative mutant of the white-rot basidiomycete Phanerochaete chrysosporium.
Auteurs : K. Boominathan [États-Unis] ; S B Dass ; T A Randall ; R L Kelley ; C A ReddySource :
- Journal of bacteriology [ 0021-9193 ] ; 1990.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Glucose oxidase, Peroxidases.
- enzymologie : Basidiomycota.
- métabolisme : Lignine.
- Mutation.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Glucose Oxidase, Peroxidases.
- enzymology : Basidiomycota.
- chemical , metabolism : Lignin.
- Mutation.
Abstract
Phanerochaete chrysosporium produces two classes of extracellular heme proteins, designated lignin peroxidases and manganese peroxidases, that play a key role in lignin degradation. In this study we isolated and characterized a lignin peroxidase-negative mutant (lip mutant) that showed 16% of the ligninolytic activity (14C-labeled synthetic lignin----14CO2) exhibited by the wild type. The lip mutant did not produce detectable levels of lignin peroxidase, whereas the wild type, under identical conditions, produced 96 U of lignin peroxidase per liter. Both the wild type and the mutant produced comparable levels of manganese peroxidase and glucose oxidase, a key H2O2-generating secondary metabolic enzyme in P. chrysosporium. Fast protein liquid chromatographic analysis of the concentrated extracellular fluid of the lip mutant confirmed that it produced only heme proteins with manganese peroxidase activity but no detectable lignin peroxidase activity, whereas both lignin peroxidase and manganese peroxidase activities were produced by the wild type. The lip mutant appears to be a regulatory mutant that is defective in the production of all the lignin peroxidases.
DOI: 10.1128/jb.172.1.260-265.1990
PubMed: 2294087
PubMed Central: PMC208426
Affiliations:
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Boominathan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.</nlm:affiliation><orgName type="university">Université d'État du Michigan</orgName><country>États-Unis</country><placeName><settlement type="city">East Lansing</settlement><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Dass, S B" sort="Dass, S B" uniqKey="Dass S" first="S B" last="Dass">S B Dass</name></author><author><name sortKey="Randall, T A" sort="Randall, T A" uniqKey="Randall T" first="T A" last="Randall">T A Randall</name></author><author><name sortKey="Kelley, R L" sort="Kelley, R L" uniqKey="Kelley R" first="R L" last="Kelley">R L Kelley</name></author><author><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1990">1990</date><idno type="RBID">pubmed:2294087</idno><idno type="pmid">2294087</idno><idno type="pmc">PMC208426</idno><idno type="doi">10.1128/jb.172.1.260-265.1990</idno><idno type="wicri:Area/Main/Corpus">000F75</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000F75</idno><idno type="wicri:Area/Main/Curation">000F75</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000F75</idno><idno type="wicri:Area/Main/Exploration">000F75</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Lignin peroxidase-negative mutant of the white-rot basidiomycete Phanerochaete chrysosporium.</title><author><name sortKey="Boominathan, K" sort="Boominathan, K" uniqKey="Boominathan K" first="K" last="Boominathan">K. Boominathan</name><affiliation wicri:level="4"><nlm:affiliation>Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.</nlm:affiliation><orgName type="university">Université d'État du Michigan</orgName><country>États-Unis</country><placeName><settlement type="city">East Lansing</settlement><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Dass, S B" sort="Dass, S B" uniqKey="Dass S" first="S B" last="Dass">S B Dass</name></author><author><name sortKey="Randall, T A" sort="Randall, T A" uniqKey="Randall T" first="T A" last="Randall">T A Randall</name></author><author><name sortKey="Kelley, R L" sort="Kelley, R L" uniqKey="Kelley R" first="R L" last="Kelley">R L Kelley</name></author><author><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></author></analytic><series><title level="j">Journal of bacteriology</title><idno type="ISSN">0021-9193</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (enzymology)</term><term>Glucose Oxidase (analysis)</term><term>Lignin (metabolism)</term><term>Mutation (MeSH)</term><term>Peroxidases (analysis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (enzymologie)</term><term>Glucose oxidase (analyse)</term><term>Lignine (métabolisme)</term><term>Mutation (MeSH)</term><term>Peroxidases (analyse)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Glucose Oxidase</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glucose oxidase</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Mutation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Mutation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phanerochaete chrysosporium produces two classes of extracellular heme proteins, designated lignin peroxidases and manganese peroxidases, that play a key role in lignin degradation. In this study we isolated and characterized a lignin peroxidase-negative mutant (lip mutant) that showed 16% of the ligninolytic activity (14C-labeled synthetic lignin----14CO2) exhibited by the wild type. The lip mutant did not produce detectable levels of lignin peroxidase, whereas the wild type, under identical conditions, produced 96 U of lignin peroxidase per liter. Both the wild type and the mutant produced comparable levels of manganese peroxidase and glucose oxidase, a key H2O2-generating secondary metabolic enzyme in P. chrysosporium. Fast protein liquid chromatographic analysis of the concentrated extracellular fluid of the lip mutant confirmed that it produced only heme proteins with manganese peroxidase activity but no detectable lignin peroxidase activity, whereas both lignin peroxidase and manganese peroxidase activities were produced by the wild type. The lip mutant appears to be a regulatory mutant that is defective in the production of all the lignin peroxidases.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2294087</PMID><DateCompleted><Year>1990</Year><Month>02</Month><Day>08</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>08</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0021-9193</ISSN><JournalIssue CitedMedium="Print"><Volume>172</Volume><Issue>1</Issue><PubDate><Year>1990</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of bacteriology</Title><ISOAbbreviation>J Bacteriol</ISOAbbreviation></Journal><ArticleTitle>Lignin peroxidase-negative mutant of the white-rot basidiomycete Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>260-5</MedlinePgn></Pagination><Abstract><AbstractText>Phanerochaete chrysosporium produces two classes of extracellular heme proteins, designated lignin peroxidases and manganese peroxidases, that play a key role in lignin degradation. In this study we isolated and characterized a lignin peroxidase-negative mutant (lip mutant) that showed 16% of the ligninolytic activity (14C-labeled synthetic lignin----14CO2) exhibited by the wild type. The lip mutant did not produce detectable levels of lignin peroxidase, whereas the wild type, under identical conditions, produced 96 U of lignin peroxidase per liter. Both the wild type and the mutant produced comparable levels of manganese peroxidase and glucose oxidase, a key H2O2-generating secondary metabolic enzyme in P. chrysosporium. Fast protein liquid chromatographic analysis of the concentrated extracellular fluid of the lip mutant confirmed that it produced only heme proteins with manganese peroxidase activity but no detectable lignin peroxidase activity, whereas both lignin peroxidase and manganese peroxidase activities were produced by the wild type. The lip mutant appears to be a regulatory mutant that is defective in the production of all the lignin peroxidases.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Boominathan</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dass</LastName><ForeName>S B</ForeName><Initials>SB</Initials></Author><Author ValidYN="Y"><LastName>Randall</LastName><ForeName>T A</ForeName><Initials>TA</Initials></Author><Author ValidYN="Y"><LastName>Kelley</LastName><ForeName>R L</ForeName><Initials>RL</Initials></Author><Author ValidYN="Y"><LastName>Reddy</LastName><ForeName>C A</ForeName><Initials>CA</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1 RO1 GM39032-01</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH 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sort="Dass, S B" uniqKey="Dass S" first="S B" last="Dass">S B Dass</name><name sortKey="Kelley, R L" sort="Kelley, R L" uniqKey="Kelley R" first="R L" last="Kelley">R L Kelley</name><name sortKey="Randall, T A" sort="Randall, T A" uniqKey="Randall T" first="T A" last="Randall">T A Randall</name><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Boominathan, K" sort="Boominathan, K" uniqKey="Boominathan K" first="K" last="Boominathan">K. 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