Serveur d'exploration sur le phanerochaete

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Identification of the heat shock protein of Neurospora crassa corresponding to the stress-inducible peroxidase.

Identifieur interne : 000E18 ( Main/Exploration ); précédent : 000E17; suivant : 000E19

Identification of the heat shock protein of Neurospora crassa corresponding to the stress-inducible peroxidase.

Auteurs : A. Machwe [Canada] ; M. Kapoor

Source :

RBID : pubmed:8240345

Descripteurs français

English descriptors

Abstract

Heat shock and other stress treatments, resulting in thermotolerance in Neurospora crassa cells, stimulate the induction of a peroxidase at a high level. The putative gene encoding this heat shock-induced peroxidase (hspp) has been cloned, using a cDNA clone of the manganese peroxidase of Phanerochaete chrysosporium, as a probe. Northern blot analysis of total RNA from heat-shocked cell showed the stress-dependent accumulation of a approximately 10 kb transcript. The identity of the HSP, corresponding to the heat-induced peroxidase, was established using hybrid-arrested in vitro translation.

DOI: 10.1006/bbrc.1993.2305
PubMed: 8240345


Affiliations:


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Le document en format XML

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<nlm:affiliation>Department of Biological Sciences, University of Calgary, Alberta, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Biological Sciences, University of Calgary, Alberta</wicri:regionArea>
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<name sortKey="Kapoor, M" sort="Kapoor, M" uniqKey="Kapoor M" first="M" last="Kapoor">M. Kapoor</name>
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<term>Blotting, Northern (MeSH)</term>
<term>Blotting, Southern (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>DNA, Fungal (analysis)</term>
<term>DNA, Fungal (metabolism)</term>
<term>Gene Expression (MeSH)</term>
<term>Genes, Fungal (MeSH)</term>
<term>Heat-Shock Proteins (biosynthesis)</term>
<term>Heat-Shock Proteins (genetics)</term>
<term>Hot Temperature (MeSH)</term>
<term>Methionine (metabolism)</term>
<term>Neurospora crassa (metabolism)</term>
<term>Peroxidases (biosynthesis)</term>
<term>Peroxidases (genetics)</term>
<term>Protein Biosynthesis (MeSH)</term>
<term>RNA, Fungal (analysis)</term>
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<term>ADN fongique (analyse)</term>
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<term>ARN fongique (analyse)</term>
<term>ARN fongique (biosynthèse)</term>
<term>Biosynthèse des protéines (MeSH)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Expression des gènes (MeSH)</term>
<term>Gènes fongiques (MeSH)</term>
<term>Méthionine (métabolisme)</term>
<term>Neurospora crassa (métabolisme)</term>
<term>Peroxidases (biosynthèse)</term>
<term>Peroxidases (génétique)</term>
<term>Protéines du choc thermique (biosynthèse)</term>
<term>Protéines du choc thermique (génétique)</term>
<term>Technique de Northern (MeSH)</term>
<term>Technique de Southern (MeSH)</term>
<term>Température élevée (MeSH)</term>
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<term>DNA, Fungal</term>
<term>RNA, Fungal</term>
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<term>Heat-Shock Proteins</term>
<term>Peroxidases</term>
<term>RNA, Fungal</term>
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<term>Heat-Shock Proteins</term>
<term>Peroxidases</term>
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<term>DNA, Fungal</term>
<term>Methionine</term>
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<term>ADN fongique</term>
<term>ARN fongique</term>
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<term>Protéines du choc thermique</term>
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<term>Peroxidases</term>
<term>Protéines du choc thermique</term>
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<term>Neurospora crassa</term>
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<term>Méthionine</term>
<term>Neurospora crassa</term>
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<term>Blotting, Southern</term>
<term>Cloning, Molecular</term>
<term>Gene Expression</term>
<term>Genes, Fungal</term>
<term>Hot Temperature</term>
<term>Protein Biosynthesis</term>
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<term>Biosynthèse des protéines</term>
<term>Clonage moléculaire</term>
<term>Expression des gènes</term>
<term>Gènes fongiques</term>
<term>Technique de Northern</term>
<term>Technique de Southern</term>
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<div type="abstract" xml:lang="en">Heat shock and other stress treatments, resulting in thermotolerance in Neurospora crassa cells, stimulate the induction of a peroxidase at a high level. The putative gene encoding this heat shock-induced peroxidase (hspp) has been cloned, using a cDNA clone of the manganese peroxidase of Phanerochaete chrysosporium, as a probe. Northern blot analysis of total RNA from heat-shocked cell showed the stress-dependent accumulation of a approximately 10 kb transcript. The identity of the HSP, corresponding to the heat-induced peroxidase, was established using hybrid-arrested in vitro translation.</div>
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<Title>Biochemical and biophysical research communications</Title>
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<AbstractText>Heat shock and other stress treatments, resulting in thermotolerance in Neurospora crassa cells, stimulate the induction of a peroxidase at a high level. The putative gene encoding this heat shock-induced peroxidase (hspp) has been cloned, using a cDNA clone of the manganese peroxidase of Phanerochaete chrysosporium, as a probe. Northern blot analysis of total RNA from heat-shocked cell showed the stress-dependent accumulation of a approximately 10 kb transcript. The identity of the HSP, corresponding to the heat-induced peroxidase, was established using hybrid-arrested in vitro translation.</AbstractText>
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