Identification of the heat shock protein of Neurospora crassa corresponding to the stress-inducible peroxidase.
Identifieur interne : 000E18 ( Main/Exploration ); précédent : 000E17; suivant : 000E19Identification of the heat shock protein of Neurospora crassa corresponding to the stress-inducible peroxidase.
Auteurs : A. Machwe [Canada] ; M. KapoorSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 1993.
Descripteurs français
- KwdFr :
- ADN fongique (analyse), ADN fongique (métabolisme), ARN fongique (analyse), ARN fongique (biosynthèse), Biosynthèse des protéines (MeSH), Clonage moléculaire (MeSH), Expression des gènes (MeSH), Gènes fongiques (MeSH), Méthionine (métabolisme), Neurospora crassa (métabolisme), Peroxidases (biosynthèse), Peroxidases (génétique), Protéines du choc thermique (biosynthèse), Protéines du choc thermique (génétique), Technique de Northern (MeSH), Technique de Southern (MeSH), Température élevée (MeSH).
- MESH :
- analyse : ADN fongique, ARN fongique.
- biosynthèse : ARN fongique, Peroxidases, Protéines du choc thermique.
- génétique : Peroxidases, Protéines du choc thermique.
- métabolisme : ADN fongique, Méthionine, Neurospora crassa.
- Biosynthèse des protéines, Clonage moléculaire, Expression des gènes, Gènes fongiques, Technique de Northern, Technique de Southern, Température élevée.
English descriptors
- KwdEn :
- Blotting, Northern (MeSH), Blotting, Southern (MeSH), Cloning, Molecular (MeSH), DNA, Fungal (analysis), DNA, Fungal (metabolism), Gene Expression (MeSH), Genes, Fungal (MeSH), Heat-Shock Proteins (biosynthesis), Heat-Shock Proteins (genetics), Hot Temperature (MeSH), Methionine (metabolism), Neurospora crassa (metabolism), Peroxidases (biosynthesis), Peroxidases (genetics), Protein Biosynthesis (MeSH), RNA, Fungal (analysis), RNA, Fungal (biosynthesis).
- MESH :
- chemical , analysis : DNA, Fungal, RNA, Fungal.
- chemical , biosynthesis : Heat-Shock Proteins, Peroxidases, RNA, Fungal.
- chemical , genetics : Heat-Shock Proteins, Peroxidases.
- chemical , metabolism : DNA, Fungal, Methionine.
- metabolism : Neurospora crassa.
- Blotting, Northern, Blotting, Southern, Cloning, Molecular, Gene Expression, Genes, Fungal, Hot Temperature, Protein Biosynthesis.
Abstract
Heat shock and other stress treatments, resulting in thermotolerance in Neurospora crassa cells, stimulate the induction of a peroxidase at a high level. The putative gene encoding this heat shock-induced peroxidase (hspp) has been cloned, using a cDNA clone of the manganese peroxidase of Phanerochaete chrysosporium, as a probe. Northern blot analysis of total RNA from heat-shocked cell showed the stress-dependent accumulation of a approximately 10 kb transcript. The identity of the HSP, corresponding to the heat-induced peroxidase, was established using hybrid-arrested in vitro translation.
DOI: 10.1006/bbrc.1993.2305
PubMed: 8240345
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<author><name sortKey="Machwe, A" sort="Machwe, A" uniqKey="Machwe A" first="A" last="Machwe">A. Machwe</name>
<affiliation wicri:level="4"><nlm:affiliation>Department of Biological Sciences, University of Calgary, Alberta, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Biological Sciences, University of Calgary, Alberta</wicri:regionArea>
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<author><name sortKey="Kapoor, M" sort="Kapoor, M" uniqKey="Kapoor M" first="M" last="Kapoor">M. Kapoor</name>
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<term>Blotting, Southern (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>DNA, Fungal (analysis)</term>
<term>DNA, Fungal (metabolism)</term>
<term>Gene Expression (MeSH)</term>
<term>Genes, Fungal (MeSH)</term>
<term>Heat-Shock Proteins (biosynthesis)</term>
<term>Heat-Shock Proteins (genetics)</term>
<term>Hot Temperature (MeSH)</term>
<term>Methionine (metabolism)</term>
<term>Neurospora crassa (metabolism)</term>
<term>Peroxidases (biosynthesis)</term>
<term>Peroxidases (genetics)</term>
<term>Protein Biosynthesis (MeSH)</term>
<term>RNA, Fungal (analysis)</term>
<term>RNA, Fungal (biosynthesis)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (analyse)</term>
<term>ADN fongique (métabolisme)</term>
<term>ARN fongique (analyse)</term>
<term>ARN fongique (biosynthèse)</term>
<term>Biosynthèse des protéines (MeSH)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Expression des gènes (MeSH)</term>
<term>Gènes fongiques (MeSH)</term>
<term>Méthionine (métabolisme)</term>
<term>Neurospora crassa (métabolisme)</term>
<term>Peroxidases (biosynthèse)</term>
<term>Peroxidases (génétique)</term>
<term>Protéines du choc thermique (biosynthèse)</term>
<term>Protéines du choc thermique (génétique)</term>
<term>Technique de Northern (MeSH)</term>
<term>Technique de Southern (MeSH)</term>
<term>Température élevée (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Fungal</term>
<term>RNA, Fungal</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Heat-Shock Proteins</term>
<term>Peroxidases</term>
<term>RNA, Fungal</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Heat-Shock Proteins</term>
<term>Peroxidases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA, Fungal</term>
<term>Methionine</term>
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<term>ARN fongique</term>
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<term>Peroxidases</term>
<term>Protéines du choc thermique</term>
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<term>Protéines du choc thermique</term>
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<term>Méthionine</term>
<term>Neurospora crassa</term>
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<term>Blotting, Southern</term>
<term>Cloning, Molecular</term>
<term>Gene Expression</term>
<term>Genes, Fungal</term>
<term>Hot Temperature</term>
<term>Protein Biosynthesis</term>
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<term>Clonage moléculaire</term>
<term>Expression des gènes</term>
<term>Gènes fongiques</term>
<term>Technique de Northern</term>
<term>Technique de Southern</term>
<term>Température élevée</term>
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<front><div type="abstract" xml:lang="en">Heat shock and other stress treatments, resulting in thermotolerance in Neurospora crassa cells, stimulate the induction of a peroxidase at a high level. The putative gene encoding this heat shock-induced peroxidase (hspp) has been cloned, using a cDNA clone of the manganese peroxidase of Phanerochaete chrysosporium, as a probe. Northern blot analysis of total RNA from heat-shocked cell showed the stress-dependent accumulation of a approximately 10 kb transcript. The identity of the HSP, corresponding to the heat-induced peroxidase, was established using hybrid-arrested in vitro translation.</div>
</front>
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<Day>09</Day>
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<DateRevised><Year>2013</Year>
<Month>11</Month>
<Day>21</Day>
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<Issue>2</Issue>
<PubDate><Year>1993</Year>
<Month>Oct</Month>
<Day>29</Day>
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<Title>Biochemical and biophysical research communications</Title>
<ISOAbbreviation>Biochem Biophys Res Commun</ISOAbbreviation>
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<ArticleTitle>Identification of the heat shock protein of Neurospora crassa corresponding to the stress-inducible peroxidase.</ArticleTitle>
<Pagination><MedlinePgn>692-8</MedlinePgn>
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<Abstract><AbstractText>Heat shock and other stress treatments, resulting in thermotolerance in Neurospora crassa cells, stimulate the induction of a peroxidase at a high level. The putative gene encoding this heat shock-induced peroxidase (hspp) has been cloned, using a cDNA clone of the manganese peroxidase of Phanerochaete chrysosporium, as a probe. Northern blot analysis of total RNA from heat-shocked cell showed the stress-dependent accumulation of a approximately 10 kb transcript. The identity of the HSP, corresponding to the heat-induced peroxidase, was established using hybrid-arrested in vitro translation.</AbstractText>
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