Demonstration of Laccase in the White Rot Basidiomycete Phanerochaete chrysosporium BKM-F1767.
Identifieur interne : 000D11 ( Main/Exploration ); précédent : 000D10; suivant : 000D12Demonstration of Laccase in the White Rot Basidiomycete Phanerochaete chrysosporium BKM-F1767.
Auteurs : C. Srinivasan ; T M Dsouza ; K. Boominathan ; C A ReddySource :
- Applied and environmental microbiology [ 0099-2240 ] ; 1995.
Abstract
It has been widely reported that the white rot basidiomycete Phanerochaete chrysosporium, unlike most other white rot fungi, does not produce laccase, an enzyme implicated in lignin biodegradation. Our results showed that P. chrysosporium BKM-F1767 produces extracellular laccase in a defined culture medium containing cellulose (10 g/liter) and either 2.4 or 24 mM ammonium tartrate. Laccase activity was demonstrated in the concentrated extracellular culture fluids of this organism as determined by a laccase plate assay as well as a spectrophotometric assay with ABTS [2,2(prm1)-azinobis(3-ethylbenzathiazoline-6-sulfonic acid)] as the substrate. Laccase activity was observed even after addition of excess catalase to the extracellular culture fluid to destroy the endogenously produced hydrogen peroxide, indicating that the observed activity is not due to a peroxidase. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by activity staining with ABTS revealed the presence of a laccase band with an estimated M(infr) of 46,500.
DOI: 10.1128/AEM.61.12.4274-4277.1995
PubMed: 16535182
PubMed Central: PMC1388647
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Srinivasan</name></author><author><name sortKey="Dsouza, T M" sort="Dsouza, T M" uniqKey="Dsouza T" first="T M" last="Dsouza">T M Dsouza</name></author><author><name sortKey="Boominathan, K" sort="Boominathan, K" uniqKey="Boominathan K" first="K" last="Boominathan">K. Boominathan</name></author><author><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="ISSN">0099-2240</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">It has been widely reported that the white rot basidiomycete Phanerochaete chrysosporium, unlike most other white rot fungi, does not produce laccase, an enzyme implicated in lignin biodegradation. Our results showed that P. chrysosporium BKM-F1767 produces extracellular laccase in a defined culture medium containing cellulose (10 g/liter) and either 2.4 or 24 mM ammonium tartrate. Laccase activity was demonstrated in the concentrated extracellular culture fluids of this organism as determined by a laccase plate assay as well as a spectrophotometric assay with ABTS [2,2(prm1)-azinobis(3-ethylbenzathiazoline-6-sulfonic acid)] as the substrate. Laccase activity was observed even after addition of excess catalase to the extracellular culture fluid to destroy the endogenously produced hydrogen peroxide, indicating that the observed activity is not due to a peroxidase. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by activity staining with ABTS revealed the presence of a laccase band with an estimated M(infr) of 46,500.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">16535182</PMID><DateCompleted><Year>2010</Year><Month>06</Month><Day>25</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>27</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0099-2240</ISSN><JournalIssue CitedMedium="Print"><Volume>61</Volume><Issue>12</Issue><PubDate><Year>1995</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Demonstration of Laccase in the White Rot Basidiomycete Phanerochaete chrysosporium BKM-F1767.</ArticleTitle><Pagination><MedlinePgn>4274-7</MedlinePgn></Pagination><Abstract><AbstractText>It has been widely reported that the white rot basidiomycete Phanerochaete chrysosporium, unlike most other white rot fungi, does not produce laccase, an enzyme implicated in lignin biodegradation. Our results showed that P. chrysosporium BKM-F1767 produces extracellular laccase in a defined culture medium containing cellulose (10 g/liter) and either 2.4 or 24 mM ammonium tartrate. Laccase activity was demonstrated in the concentrated extracellular culture fluids of this organism as determined by a laccase plate assay as well as a spectrophotometric assay with ABTS [2,2(prm1)-azinobis(3-ethylbenzathiazoline-6-sulfonic acid)] as the substrate. Laccase activity was observed even after addition of excess catalase to the extracellular culture fluid to destroy the endogenously produced hydrogen peroxide, indicating that the observed activity is not due to a peroxidase. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by activity staining with ABTS revealed the presence of a laccase band with an estimated M(infr) of 46,500.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Srinivasan</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Dsouza</LastName><ForeName>T M</ForeName><Initials>TM</Initials></Author><Author ValidYN="Y"><LastName>Boominathan</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Reddy</LastName><ForeName>C A</ForeName><Initials>CA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Environ Microbiol</MedlineTA><NlmUniqueID>7605801</NlmUniqueID><ISSNLinking>0099-2240</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1995</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1995</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1995</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16535182</ArticleId><ArticleId IdType="pmc">PMC1388647</ArticleId><ArticleId IdType="doi">10.1128/AEM.61.12.4274-4277.1995</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Bacteriol. 1990 Jan;172(1):260-5</Citation><ArticleIdList><ArticleId IdType="pubmed">2294087</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 1988 Sep 15;254(3):877-83</Citation><ArticleIdList><ArticleId IdType="pubmed">3196301</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Microbiol. 1987;41:465-505</Citation><ArticleIdList><ArticleId IdType="pubmed">3318677</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycologia. 1974 May-Jun;66(3):469-76</Citation><ArticleIdList><ArticleId IdType="pubmed">4210374</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 1970 Aug 15;227(5259):680-5</Citation><ArticleIdList><ArticleId IdType="pubmed">5432063</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Microbiol. 1995 Apr;163(4):254-8</Citation><ArticleIdList><ArticleId IdType="pubmed">7763133</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biotechnol. 1993 Jul;30(1):91-107</Citation><ArticleIdList><ArticleId IdType="pubmed">7763835</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1995 Mar;61(3):872-6</Citation><ArticleIdList><ArticleId IdType="pubmed">7793921</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1995 Jan;61(1):341-5</Citation><ArticleIdList><ArticleId IdType="pubmed">7887613</ArticleId></ArticleIdList></Reference><Reference><Citation>Ital J Biochem. 1994 Jan-Feb;43(1):24-8</Citation><ArticleIdList><ArticleId IdType="pubmed">8206741</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiol Rev. 1993 Sep;57(3):605-22</Citation><ArticleIdList><ArticleId IdType="pubmed">8246842</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Microbiol. 1993;160(1):1-4</Citation><ArticleIdList><ArticleId IdType="pubmed">8352647</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1993 Aug;59(8):2607-13</Citation><ArticleIdList><ArticleId IdType="pubmed">8368848</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1995 Mar;61(3):907-12</Citation><ArticleIdList><ArticleId IdType="pubmed">16534974</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1993 Apr 30;260(5108):672-4</Citation><ArticleIdList><ArticleId IdType="pubmed">17812228</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Boominathan, K" sort="Boominathan, K" uniqKey="Boominathan K" first="K" last="Boominathan">K. Boominathan</name><name sortKey="Dsouza, T M" sort="Dsouza, T M" uniqKey="Dsouza T" first="T M" last="Dsouza">T M Dsouza</name><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name><name sortKey="Srinivasan, C" sort="Srinivasan, C" uniqKey="Srinivasan C" first="C" last="Srinivasan">C. Srinivasan</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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