Co-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene in Saccharomyces cerevisiae.
Identifieur interne : 000C63 ( Main/Exploration ); précédent : 000C62; suivant : 000C64Co-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene in Saccharomyces cerevisiae.
Auteurs : P. Van Rensburg [Afrique du Sud] ; W H Van Zyl ; I S PretoriusSource :
- Current genetics [ 0172-8083 ] ; 1996.
Descripteurs français
- KwdFr :
- Agaricales (enzymologie), Agaricales (génétique), Amorces ADN (MeSH), Bactéries anaérobies à Gram négatif (enzymologie), Bactéries anaérobies à Gram négatif (génétique), Cartographie de restriction (MeSH), Cellulase (biosynthèse), Cellulase (génétique), Cellulose 1,4-beta-cellobiosidase (MeSH), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Expression des gènes (MeSH), Glycosidases (biosynthèse), Glycosidases (génétique), Gènes bactériens (MeSH), Gènes fongiques (MeSH), Génotype (MeSH), Plasmides (MeSH), Protéines recombinantes (biosynthèse), Réaction de polymérisation en chaîne (MeSH), Saccharomyces cerevisiae (génétique), Séquence nucléotidique (MeSH).
- MESH :
- biosynthèse : Cellulase, Glycosidases, Protéines recombinantes.
- enzymologie : Agaricales, Bactéries anaérobies à Gram négatif.
- génétique : Agaricales, Bactéries anaérobies à Gram négatif, Cellulase, Glycosidases, Saccharomyces cerevisiae.
- Amorces ADN, Cartographie de restriction, Cellulose 1,4-beta-cellobiosidase, Clonage moléculaire, Données de séquences moléculaires, Expression des gènes, Gènes bactériens, Gènes fongiques, Génotype, Plasmides, Réaction de polymérisation en chaîne, Séquence nucléotidique.
English descriptors
- KwdEn :
- Agaricales (enzymology), Agaricales (genetics), Base Sequence (MeSH), Cellulase (biosynthesis), Cellulase (genetics), Cellulose 1,4-beta-Cellobiosidase (MeSH), Cloning, Molecular (MeSH), DNA Primers (MeSH), Gene Expression (MeSH), Genes, Bacterial (MeSH), Genes, Fungal (MeSH), Genotype (MeSH), Glycoside Hydrolases (biosynthesis), Glycoside Hydrolases (genetics), Gram-Negative Anaerobic Bacteria (enzymology), Gram-Negative Anaerobic Bacteria (genetics), Molecular Sequence Data (MeSH), Plasmids (MeSH), Polymerase Chain Reaction (MeSH), Recombinant Proteins (biosynthesis), Restriction Mapping (MeSH), Saccharomyces cerevisiae (genetics).
- MESH :
- chemical , biosynthesis : Cellulase, Glycoside Hydrolases, Recombinant Proteins.
- enzymology : Agaricales, Gram-Negative Anaerobic Bacteria.
- genetics : Agaricales, Cellulase, Glycoside Hydrolases, Gram-Negative Anaerobic Bacteria, Saccharomyces cerevisiae.
- Base Sequence, Cellulose 1,4-beta-Cellobiosidase, Cloning, Molecular, DNA Primers, Gene Expression, Genes, Bacterial, Genes, Fungal, Genotype, Molecular Sequence Data, Plasmids, Polymerase Chain Reaction, Restriction Mapping.
Abstract
A cDNA fragment encoding the Phanerochaete chrysosporium cellobiohydrolase (cbh1-4) was amplified and cloned with the aid of the polymerase chain reaction (PCR) technique. The cbh1-4 gene and the Butyrivibrio fibrisolvens endo-beta-1,4-glucanase (end1) gene were successfully expressed in Saccharomyces cerevisiae under the control of the phosphoglycerate kinase-I (PGK1) and alcohol dehydrogenase-II (ADH2) gene promoters and terminators, respectively. The native P. chrysosporium signal sequence mediated secretion of cellobiohydrolase in S. cerevisiae, whereas secretion of the endo-beta-1,4-glucanase was directed by the signal sequence of the yeast mating pheromone alpha-factor (MFalpha1S). These constructs, designated CBH1 and END1, respectively, were expressed separately and jointly in S. cerevisiae. The construction of fur1 ura3 S. cerevisiae strains allowed for the autoselection of these multicopy URA3-based plasmids in rich medium. Enzyme assays confirmed that co-expression of CBH1 and END1 synergistically enhanced cellulose degradation by S. cerevisiae.
DOI: 10.1007/s002940050128
PubMed: 8753654
Affiliations:
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Le document en format XML
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Van Rensburg</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Institute for Wine Biotechnology, University of Stellenbosch, Stellenbosch 7600, South Africa.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Department of Microbiology and Institute for Wine Biotechnology, University of Stellenbosch, Stellenbosch 7600</wicri:regionArea><wicri:noRegion>Stellenbosch 7600</wicri:noRegion></affiliation></author><author><name sortKey="Van Zyl, W H" sort="Van Zyl, W H" uniqKey="Van Zyl W" first="W H" last="Van Zyl">W H Van Zyl</name></author><author><name sortKey="Pretorius, I S" sort="Pretorius, I S" uniqKey="Pretorius I" first="I S" last="Pretorius">I S Pretorius</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:8753654</idno><idno type="pmid">8753654</idno><idno type="doi">10.1007/s002940050128</idno><idno type="wicri:Area/Main/Corpus">000C38</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000C38</idno><idno type="wicri:Area/Main/Curation">000C38</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000C38</idno><idno type="wicri:Area/Main/Exploration">000C38</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Co-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene in Saccharomyces cerevisiae.</title><author><name sortKey="Van Rensburg, P" sort="Van Rensburg, P" uniqKey="Van Rensburg P" first="P" last="Van Rensburg">P. Van Rensburg</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Institute for Wine Biotechnology, University of Stellenbosch, Stellenbosch 7600, South Africa.</nlm:affiliation><country xml:lang="fr">Afrique du Sud</country><wicri:regionArea>Department of Microbiology and Institute for Wine Biotechnology, University of Stellenbosch, Stellenbosch 7600</wicri:regionArea><wicri:noRegion>Stellenbosch 7600</wicri:noRegion></affiliation></author><author><name sortKey="Van Zyl, W H" sort="Van Zyl, W H" uniqKey="Van Zyl W" first="W H" last="Van Zyl">W H Van Zyl</name></author><author><name sortKey="Pretorius, I S" sort="Pretorius, I S" uniqKey="Pretorius I" first="I S" last="Pretorius">I S Pretorius</name></author></analytic><series><title level="j">Current genetics</title><idno type="ISSN">0172-8083</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agaricales (enzymology)</term><term>Agaricales (genetics)</term><term>Base Sequence (MeSH)</term><term>Cellulase (biosynthesis)</term><term>Cellulase (genetics)</term><term>Cellulose 1,4-beta-Cellobiosidase (MeSH)</term><term>Cloning, Molecular (MeSH)</term><term>DNA Primers (MeSH)</term><term>Gene Expression (MeSH)</term><term>Genes, Bacterial (MeSH)</term><term>Genes, Fungal (MeSH)</term><term>Genotype (MeSH)</term><term>Glycoside Hydrolases (biosynthesis)</term><term>Glycoside Hydrolases (genetics)</term><term>Gram-Negative Anaerobic Bacteria (enzymology)</term><term>Gram-Negative Anaerobic Bacteria (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Plasmids (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Recombinant Proteins (biosynthesis)</term><term>Restriction Mapping (MeSH)</term><term>Saccharomyces cerevisiae (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agaricales (enzymologie)</term><term>Agaricales (génétique)</term><term>Amorces ADN (MeSH)</term><term>Bactéries anaérobies à Gram négatif (enzymologie)</term><term>Bactéries anaérobies à Gram négatif (génétique)</term><term>Cartographie de restriction (MeSH)</term><term>Cellulase (biosynthèse)</term><term>Cellulase (génétique)</term><term>Cellulose 1,4-beta-cellobiosidase (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Expression des gènes (MeSH)</term><term>Glycosidases (biosynthèse)</term><term>Glycosidases (génétique)</term><term>Gènes bactériens (MeSH)</term><term>Gènes fongiques (MeSH)</term><term>Génotype (MeSH)</term><term>Plasmides (MeSH)</term><term>Protéines recombinantes (biosynthèse)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Séquence nucléotidique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Cellulase</term><term>Glycoside Hydrolases</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Cellulase</term><term>Glycosidases</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Agaricales</term><term>Bactéries anaérobies à Gram négatif</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Agaricales</term><term>Gram-Negative Anaerobic Bacteria</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agaricales</term><term>Cellulase</term><term>Glycoside Hydrolases</term><term>Gram-Negative Anaerobic Bacteria</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agaricales</term><term>Bactéries anaérobies à Gram négatif</term><term>Cellulase</term><term>Glycosidases</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Cellulose 1,4-beta-Cellobiosidase</term><term>Cloning, Molecular</term><term>DNA Primers</term><term>Gene Expression</term><term>Genes, Bacterial</term><term>Genes, Fungal</term><term>Genotype</term><term>Molecular Sequence Data</term><term>Plasmids</term><term>Polymerase Chain Reaction</term><term>Restriction Mapping</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Cartographie de restriction</term><term>Cellulose 1,4-beta-cellobiosidase</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Expression des gènes</term><term>Gènes bactériens</term><term>Gènes fongiques</term><term>Génotype</term><term>Plasmides</term><term>Réaction de polymérisation en chaîne</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A cDNA fragment encoding the Phanerochaete chrysosporium cellobiohydrolase (cbh1-4) was amplified and cloned with the aid of the polymerase chain reaction (PCR) technique. The cbh1-4 gene and the Butyrivibrio fibrisolvens endo-beta-1,4-glucanase (end1) gene were successfully expressed in Saccharomyces cerevisiae under the control of the phosphoglycerate kinase-I (PGK1) and alcohol dehydrogenase-II (ADH2) gene promoters and terminators, respectively. The native P. chrysosporium signal sequence mediated secretion of cellobiohydrolase in S. cerevisiae, whereas secretion of the endo-beta-1,4-glucanase was directed by the signal sequence of the yeast mating pheromone alpha-factor (MFalpha1S). These constructs, designated CBH1 and END1, respectively, were expressed separately and jointly in S. cerevisiae. The construction of fur1 ura3 S. cerevisiae strains allowed for the autoselection of these multicopy URA3-based plasmids in rich medium. Enzyme assays confirmed that co-expression of CBH1 and END1 synergistically enhanced cellulose degradation by S. cerevisiae.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8753654</PMID><DateCompleted><Year>1996</Year><Month>11</Month><Day>05</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0172-8083</ISSN><JournalIssue CitedMedium="Print"><Volume>30</Volume><Issue>3</Issue><PubDate><Year>1996</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Current genetics</Title><ISOAbbreviation>Curr Genet</ISOAbbreviation></Journal><ArticleTitle>Co-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene in Saccharomyces cerevisiae.</ArticleTitle><Pagination><MedlinePgn>246-50</MedlinePgn></Pagination><Abstract><AbstractText>A cDNA fragment encoding the Phanerochaete chrysosporium cellobiohydrolase (cbh1-4) was amplified and cloned with the aid of the polymerase chain reaction (PCR) technique. The cbh1-4 gene and the Butyrivibrio fibrisolvens endo-beta-1,4-glucanase (end1) gene were successfully expressed in Saccharomyces cerevisiae under the control of the phosphoglycerate kinase-I (PGK1) and alcohol dehydrogenase-II (ADH2) gene promoters and terminators, respectively. The native P. chrysosporium signal sequence mediated secretion of cellobiohydrolase in S. cerevisiae, whereas secretion of the endo-beta-1,4-glucanase was directed by the signal sequence of the yeast mating pheromone alpha-factor (MFalpha1S). These constructs, designated CBH1 and END1, respectively, were expressed separately and jointly in S. cerevisiae. The construction of fur1 ura3 S. cerevisiae strains allowed for the autoselection of these multicopy URA3-based plasmids in rich medium. Enzyme assays confirmed that co-expression of CBH1 and END1 synergistically enhanced cellulose degradation by S. cerevisiae.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>van Rensburg</LastName><ForeName>P</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Institute for Wine Biotechnology, University of Stellenbosch, Stellenbosch 7600, South Africa.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van Zyl</LastName><ForeName>W H</ForeName><Initials>WH</Initials></Author><Author ValidYN="Y"><LastName>Pretorius</LastName><ForeName>I S</ForeName><Initials>IS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Curr Genet</MedlineTA><NlmUniqueID>8004904</NlmUniqueID><ISSNLinking>0172-8083</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D006026">Glycoside Hydrolases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.73</RegistryNumber><NameOfSubstance UI="C028020">licheninase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.91</RegistryNumber><NameOfSubstance UI="D043366">Cellulose 1,4-beta-Cellobiosidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000363" MajorTopicYN="N">Agaricales</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D043366" MajorTopicYN="N">Cellulose 1,4-beta-Cellobiosidase</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005798" MajorTopicYN="Y">Genes, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="Y">Genes, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006026" MajorTopicYN="N">Glycoside Hydrolases</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006089" MajorTopicYN="N">Gram-Negative Anaerobic Bacteria</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015183" MajorTopicYN="N">Restriction Mapping</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1996</Year><Month>8</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1996</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1996</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8753654</ArticleId><ArticleId IdType="doi">10.1007/s002940050128</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Afrique du Sud</li></country></list><tree><noCountry><name sortKey="Pretorius, I S" sort="Pretorius, I S" uniqKey="Pretorius I" first="I S" last="Pretorius">I S Pretorius</name><name sortKey="Van Zyl, W H" sort="Van Zyl, W H" uniqKey="Van Zyl W" first="W H" last="Van Zyl">W H Van Zyl</name></noCountry><country name="Afrique du Sud"><noRegion><name sortKey="Van Rensburg, P" sort="Van Rensburg, P" uniqKey="Van Rensburg P" first="P" last="Van Rensburg">P. 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