Enhanced production of cellobiose dehydrogenase in cultures of Phanerochaete chrysosporium supplemented with bovine calf serum.
Identifieur interne : 000C13 ( Main/Exploration ); précédent : 000C12; suivant : 000C14Enhanced production of cellobiose dehydrogenase in cultures of Phanerochaete chrysosporium supplemented with bovine calf serum.
Auteurs : N. Habu [États-Unis] ; K. Igarashi ; M. Samejima ; B. Pettersson ; K E ErikssonSource :
- Biotechnology and applied biochemistry [ 0885-4513 ] ; 1997.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Basidiomycota (croissance et développement), Basidiomycota (enzymologie), Basidiomycota (métabolisme), Biotechnologie (MeSH), Bovins (MeSH), Carbohydrate dehydrogenases (biosynthèse), Carbohydrate dehydrogenases (métabolisme), Cellulase (biosynthèse), Cellulose (métabolisme), Dépollution biologique de l'environnement (MeSH), Glycosidases (biosynthèse), Milieux de culture (MeSH), Stabilité enzymatique (MeSH), Sérumalbumine bovine (MeSH).
- MESH :
- biosynthèse : Carbohydrate dehydrogenases, Cellulase, Glycosidases.
- croissance et développement : Basidiomycota.
- enzymologie : Basidiomycota.
- métabolisme : Basidiomycota, Carbohydrate dehydrogenases, Cellulose.
- Animaux, Biotechnologie, Bovins, Dépollution biologique de l'environnement, Milieux de culture, Stabilité enzymatique, Sérumalbumine bovine.
English descriptors
- KwdEn :
- Animals (MeSH), Basidiomycota (enzymology), Basidiomycota (growth & development), Basidiomycota (metabolism), Biodegradation, Environmental (MeSH), Biotechnology (MeSH), Carbohydrate Dehydrogenases (biosynthesis), Carbohydrate Dehydrogenases (metabolism), Cattle (MeSH), Cellulase (biosynthesis), Cellulose (metabolism), Culture Media (MeSH), Enzyme Stability (MeSH), Glycoside Hydrolases (biosynthesis), Serum Albumin, Bovine (MeSH).
- MESH :
- chemical , biosynthesis : Carbohydrate Dehydrogenases, Cellulase, Glycoside Hydrolases.
- enzymology : Basidiomycota.
- growth & development : Basidiomycota.
- metabolism : Basidiomycota, Carbohydrate Dehydrogenases, Cellulose.
- Animals, Biodegradation, Environmental, Biotechnology, Cattle, Culture Media, Enzyme Stability, Serum Albumin, Bovine.
Abstract
The enzyme cellobiose dehydrogenase (CDH), produced by many wood-degrading fungi has, in recent years, attracted considerable interest for its possible role in both cellulose and lignin degradation. To characterize the enzyme better and to identify its role in the degradation of wood and wood components, it is desirable to produce it in higher amounts. We report here that the addition of bovine calf serum to cellulose-grown cultures of Phanerochaete chrysosporium enhances the production of certain enzymes, CDH in particular. The highest CDH production was obtained with 45 ml of serum/litre of medium added on day 3 or 4. The resultant CDH yield was approx. 700-800 units/litre, which was 3.5-4 times higher than that in cultures without serum. Serum addition also enhanced the production of beta-glucosidase. However, the impact on CDH production was the most dramatic. The enhanced enzyme production cannot be explained by increased rates of spore germination, simple nutrient effects or cofactor effects. Fractionation of serum by Cohn's fractionation technique showed that the albumin (BSA) fraction had almost the same effect as whole serum. However, purified BSA had less effect than crude BSA (fraction V of Cohn's fractions), suggesting that an additional factor, probably a protease inhibitor in serum, also contributed to the effect of serum.
PubMed: 9357105
Affiliations:
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Samejima</name></author><author><name sortKey="Pettersson, B" sort="Pettersson, B" uniqKey="Pettersson B" first="B" last="Pettersson">B. Pettersson</name></author><author><name sortKey="Eriksson, K E" sort="Eriksson, K E" uniqKey="Eriksson K" first="K E" last="Eriksson">K E Eriksson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1997">1997</date><idno type="RBID">pubmed:9357105</idno><idno type="pmid">9357105</idno><idno type="wicri:Area/Main/Corpus">000B98</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000B98</idno><idno type="wicri:Area/Main/Curation">000B98</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000B98</idno><idno type="wicri:Area/Main/Exploration">000B98</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Enhanced production of cellobiose dehydrogenase in cultures of Phanerochaete chrysosporium supplemented with bovine calf serum.</title><author><name sortKey="Habu, N" sort="Habu, N" uniqKey="Habu N" first="N" last="Habu">N. 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Pettersson</name></author><author><name sortKey="Eriksson, K E" sort="Eriksson, K E" uniqKey="Eriksson K" first="K E" last="Eriksson">K E Eriksson</name></author></analytic><series><title level="j">Biotechnology and applied biochemistry</title><idno type="ISSN">0885-4513</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Basidiomycota (enzymology)</term><term>Basidiomycota (growth & development)</term><term>Basidiomycota (metabolism)</term><term>Biodegradation, Environmental (MeSH)</term><term>Biotechnology (MeSH)</term><term>Carbohydrate Dehydrogenases (biosynthesis)</term><term>Carbohydrate Dehydrogenases (metabolism)</term><term>Cattle (MeSH)</term><term>Cellulase (biosynthesis)</term><term>Cellulose (metabolism)</term><term>Culture Media (MeSH)</term><term>Enzyme Stability (MeSH)</term><term>Glycoside Hydrolases (biosynthesis)</term><term>Serum Albumin, Bovine (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Basidiomycota (croissance et développement)</term><term>Basidiomycota (enzymologie)</term><term>Basidiomycota (métabolisme)</term><term>Biotechnologie (MeSH)</term><term>Bovins (MeSH)</term><term>Carbohydrate dehydrogenases (biosynthèse)</term><term>Carbohydrate dehydrogenases (métabolisme)</term><term>Cellulase (biosynthèse)</term><term>Cellulose (métabolisme)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Glycosidases (biosynthèse)</term><term>Milieux de culture (MeSH)</term><term>Stabilité enzymatique (MeSH)</term><term>Sérumalbumine bovine (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Carbohydrate Dehydrogenases</term><term>Cellulase</term><term>Glycoside Hydrolases</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Carbohydrate dehydrogenases</term><term>Cellulase</term><term>Glycosidases</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term><term>Carbohydrate Dehydrogenases</term><term>Cellulose</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Basidiomycota</term><term>Carbohydrate dehydrogenases</term><term>Cellulose</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Biodegradation, Environmental</term><term>Biotechnology</term><term>Cattle</term><term>Culture Media</term><term>Enzyme Stability</term><term>Serum Albumin, Bovine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Biotechnologie</term><term>Bovins</term><term>Dépollution biologique de l'environnement</term><term>Milieux de culture</term><term>Stabilité enzymatique</term><term>Sérumalbumine bovine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The enzyme cellobiose dehydrogenase (CDH), produced by many wood-degrading fungi has, in recent years, attracted considerable interest for its possible role in both cellulose and lignin degradation. To characterize the enzyme better and to identify its role in the degradation of wood and wood components, it is desirable to produce it in higher amounts. We report here that the addition of bovine calf serum to cellulose-grown cultures of Phanerochaete chrysosporium enhances the production of certain enzymes, CDH in particular. The highest CDH production was obtained with 45 ml of serum/litre of medium added on day 3 or 4. The resultant CDH yield was approx. 700-800 units/litre, which was 3.5-4 times higher than that in cultures without serum. Serum addition also enhanced the production of beta-glucosidase. However, the impact on CDH production was the most dramatic. The enhanced enzyme production cannot be explained by increased rates of spore germination, simple nutrient effects or cofactor effects. Fractionation of serum by Cohn's fractionation technique showed that the albumin (BSA) fraction had almost the same effect as whole serum. However, purified BSA had less effect than crude BSA (fraction V of Cohn's fractions), suggesting that an additional factor, probably a protease inhibitor in serum, also contributed to the effect of serum.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9357105</PMID><DateCompleted><Year>1998</Year><Month>01</Month><Day>06</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0885-4513</ISSN><JournalIssue CitedMedium="Print"><Volume>26</Volume><Issue>2</Issue><PubDate><Year>1997</Year><Month>10</Month></PubDate></JournalIssue><Title>Biotechnology and applied biochemistry</Title><ISOAbbreviation>Biotechnol Appl Biochem</ISOAbbreviation></Journal><ArticleTitle>Enhanced production of cellobiose dehydrogenase in cultures of Phanerochaete chrysosporium supplemented with bovine calf serum.</ArticleTitle><Pagination><MedlinePgn>97-102</MedlinePgn></Pagination><Abstract><AbstractText>The enzyme cellobiose dehydrogenase (CDH), produced by many wood-degrading fungi has, in recent years, attracted considerable interest for its possible role in both cellulose and lignin degradation. To characterize the enzyme better and to identify its role in the degradation of wood and wood components, it is desirable to produce it in higher amounts. We report here that the addition of bovine calf serum to cellulose-grown cultures of Phanerochaete chrysosporium enhances the production of certain enzymes, CDH in particular. The highest CDH production was obtained with 45 ml of serum/litre of medium added on day 3 or 4. The resultant CDH yield was approx. 700-800 units/litre, which was 3.5-4 times higher than that in cultures without serum. Serum addition also enhanced the production of beta-glucosidase. However, the impact on CDH production was the most dramatic. The enhanced enzyme production cannot be explained by increased rates of spore germination, simple nutrient effects or cofactor effects. Fractionation of serum by Cohn's fractionation technique showed that the albumin (BSA) fraction had almost the same effect as whole serum. However, purified BSA had less effect than crude BSA (fraction V of Cohn's fractions), suggesting that an additional factor, probably a protease inhibitor in serum, also contributed to the effect of serum.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Habu</LastName><ForeName>N</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Igarashi</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Samejima</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Pettersson</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Eriksson</LastName><ForeName>K E</ForeName><Initials>KE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Appl Biochem</MedlineTA><NlmUniqueID>8609465</NlmUniqueID><ISSNLinking>0885-4513</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>27432CM55Q</RegistryNumber><NameOfSubstance UI="D012710">Serum Albumin, Bovine</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.-</RegistryNumber><NameOfSubstance UI="D002237">Carbohydrate Dehydrogenases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.99.18</RegistryNumber><NameOfSubstance UI="C019859">cellobiose-quinone oxidoreductase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D006026">Glycoside Hydrolases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="C023305">hemicellulase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002237" MajorTopicYN="N">Carbohydrate Dehydrogenases</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002417" MajorTopicYN="N">Cattle</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004795" MajorTopicYN="N">Enzyme Stability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006026" MajorTopicYN="N">Glycoside Hydrolases</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012710" MajorTopicYN="N">Serum Albumin, Bovine</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1997</Year><Month>11</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1997</Year><Month>11</Month><Day>14</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1997</Year><Month>11</Month><Day>14</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9357105</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Eriksson, K E" sort="Eriksson, K E" uniqKey="Eriksson K" first="K E" last="Eriksson">K E Eriksson</name><name sortKey="Igarashi, K" sort="Igarashi, K" uniqKey="Igarashi K" first="K" last="Igarashi">K. Igarashi</name><name sortKey="Pettersson, B" sort="Pettersson, B" uniqKey="Pettersson B" first="B" last="Pettersson">B. Pettersson</name><name sortKey="Samejima, M" sort="Samejima, M" uniqKey="Samejima M" first="M" last="Samejima">M. Samejima</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Habu, N" sort="Habu, N" uniqKey="Habu N" first="N" last="Habu">N. Habu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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