A method of fast separation of lignin peroxidases using convective interaction media disks.
Identifieur interne : 000B55 ( Main/Exploration ); précédent : 000B54; suivant : 000B56A method of fast separation of lignin peroxidases using convective interaction media disks.
Auteurs : H. Podgornik [Slovénie] ; A. Podgornik ; A. PerdihSource :
- Analytical biochemistry [ 0003-2697 ] ; 1999.
Descripteurs français
- KwdFr :
- Chromatographie en phase liquide à haute performance (instrumentation), Chromatographie en phase liquide à haute performance (méthodes), Chromatographie en phase liquide à haute performance (statistiques et données numériques), Focalisation isoélectrique (MeSH), Isoenzymes (composition chimique), Isoenzymes (isolement et purification), Peroxidases (composition chimique), Peroxidases (isolement et purification), Phanerochaete (enzymologie), Point isoélectrique (MeSH), Reproductibilité des résultats (MeSH), Études d'évaluation comme sujet (MeSH).
- MESH :
- composition chimique : Isoenzymes, Peroxidases.
- enzymologie : Phanerochaete.
- isolement et purification : Isoenzymes, Peroxidases.
- méthodes : Chromatographie en phase liquide à haute performance.
- statistiques et données numériques : Chromatographie en phase liquide à haute performance.
- instrumentation : Chromatographie en phase liquide à haute performance, Focalisation isoélectrique, Point isoélectrique, Reproductibilité des résultats, Études d'évaluation comme sujet.
English descriptors
- KwdEn :
- Chromatography, High Pressure Liquid (instrumentation), Chromatography, High Pressure Liquid (methods), Chromatography, High Pressure Liquid (statistics & numerical data), Evaluation Studies as Topic (MeSH), Isoelectric Focusing (MeSH), Isoelectric Point (MeSH), Isoenzymes (chemistry), Isoenzymes (isolation & purification), Peroxidases (chemistry), Peroxidases (isolation & purification), Phanerochaete (enzymology), Reproducibility of Results (MeSH).
- MESH :
- chemical , chemistry : Isoenzymes, Peroxidases.
- enzymology : Phanerochaete.
- instrumentation : Chromatography, High Pressure Liquid.
- chemical , isolation & purification : Isoenzymes, Peroxidases.
- methods : Chromatography, High Pressure Liquid.
- statistics & numerical data : Chromatography, High Pressure Liquid.
- Evaluation Studies as Topic, Isoelectric Focusing, Isoelectric Point, Reproducibility of Results.
Abstract
The HPLC separation of lignin peroxidase isoenzymes using Convective Interaction Media disks containing quaternary amine and diethylaminoethyl ion-exchange active groups is proposed. In contrast to standard HPLC procedures the separation can be performed within a few minutes without considerably affecting the separation resolution. The method is reproducible and gives a linear response of integrated peak area to protein concentration for all measured isoenzymes. The separation resolution is retained unchanged by applying crude culture filtrate instead of a sample previously frozen and dialyzed. The optimized method might therefore be used for on-line monitoring of lignin peroxidase isoenzyme composition during fermentation. On the other hand, the proposed method is comparable in time to the original method of lignin peroxidase activity measurement (proposed by Tien and Kirk), providing additionally the isoenzyme composition.
DOI: 10.1006/abio.1999.4146
PubMed: 10405291
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Perdih</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1999">1999</date><idno type="RBID">pubmed:10405291</idno><idno type="pmid">10405291</idno><idno type="doi">10.1006/abio.1999.4146</idno><idno type="wicri:Area/Main/Corpus">000B36</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000B36</idno><idno type="wicri:Area/Main/Curation">000B36</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000B36</idno><idno type="wicri:Area/Main/Exploration">000B36</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A method of fast separation of lignin peroxidases using convective interaction media disks.</title><author><name sortKey="Podgornik, H" sort="Podgornik, H" uniqKey="Podgornik H" first="H" last="Podgornik">H. 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In contrast to standard HPLC procedures the separation can be performed within a few minutes without considerably affecting the separation resolution. The method is reproducible and gives a linear response of integrated peak area to protein concentration for all measured isoenzymes. The separation resolution is retained unchanged by applying crude culture filtrate instead of a sample previously frozen and dialyzed. The optimized method might therefore be used for on-line monitoring of lignin peroxidase isoenzyme composition during fermentation. On the other hand, the proposed method is comparable in time to the original method of lignin peroxidase activity measurement (proposed by Tien and Kirk), providing additionally the isoenzyme composition.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10405291</PMID><DateCompleted><Year>1999</Year><Month>09</Month><Day>10</Day></DateCompleted><DateRevised><Year>2007</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0003-2697</ISSN><JournalIssue CitedMedium="Print"><Volume>272</Volume><Issue>1</Issue><PubDate><Year>1999</Year><Month>Jul</Month><Day>15</Day></PubDate></JournalIssue><Title>Analytical biochemistry</Title><ISOAbbreviation>Anal Biochem</ISOAbbreviation></Journal><ArticleTitle>A method of fast separation of lignin peroxidases using convective interaction media disks.</ArticleTitle><Pagination><MedlinePgn>43-7</MedlinePgn></Pagination><Abstract><AbstractText>The HPLC separation of lignin peroxidase isoenzymes using Convective Interaction Media disks containing quaternary amine and diethylaminoethyl ion-exchange active groups is proposed. In contrast to standard HPLC procedures the separation can be performed within a few minutes without considerably affecting the separation resolution. The method is reproducible and gives a linear response of integrated peak area to protein concentration for all measured isoenzymes. The separation resolution is retained unchanged by applying crude culture filtrate instead of a sample previously frozen and dialyzed. The optimized method might therefore be used for on-line monitoring of lignin peroxidase isoenzyme composition during fermentation. 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Perdih</name><name sortKey="Podgornik, A" sort="Podgornik, A" uniqKey="Podgornik A" first="A" last="Podgornik">A. Podgornik</name></noCountry><country name="Slovénie"><noRegion><name sortKey="Podgornik, H" sort="Podgornik, H" uniqKey="Podgornik H" first="H" last="Podgornik">H. Podgornik</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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