Glutathione-mediated mineralization of 14C-labeled 2-amino-4,6-dinitrotoluene by manganese-dependent peroxidase H5 from the white-rot fungus Phanerochaete chrysosporium.
Identifieur interne : 000A81 ( Main/Exploration ); précédent : 000A80; suivant : 000A82Glutathione-mediated mineralization of 14C-labeled 2-amino-4,6-dinitrotoluene by manganese-dependent peroxidase H5 from the white-rot fungus Phanerochaete chrysosporium.
Auteurs : B. Van Aken [Belgique] ; M D Cameron ; J D Stahl ; A. Plumat ; H. Naveau ; S D Aust ; S N AgathosSource :
- Applied microbiology and biotechnology [ 0175-7598 ] ; 2000.
Descripteurs français
- KwdFr :
- Dépollution biologique de l'environnement (MeSH), Dérivés de l'aniline (métabolisme), Glutathion (métabolisme), Manganèse (métabolisme), Marqueurs de spin (MeSH), Oxydoréduction (MeSH), Peroxidases (métabolisme), Phanerochaete (enzymologie), Radicaux libres (MeSH), Spectroscopie de résonance de spin électronique (MeSH).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Aniline Compounds, Glutathione, Manganese, Peroxidases.
- enzymology : Phanerochaete.
- Biodegradation, Environmental, Electron Spin Resonance Spectroscopy, Free Radicals, Oxidation-Reduction, Spin Labels.
Abstract
Manganese-dependent peroxidase (MnP) H5 from the white-rot fungus Phanerochaete chrysosporium, in the presence of either Mn(II) (10 mM) or GSH (10 mM). was able to mineralize 14C-U-ring-labeled 2-amino-4,6-dinitrotoluene (2-A-4,6-DNT) up to 29% in 12 days. When both Mn(II) and GSH were present, the mineralization extent reached 82%. On the other hand, no significant mineralization was observed in the absence of both Mn(II) and GSH, suggesting the requirement of a mediator [either Mn(II) or GSH] for the degradation of 2-A-4,6-DNT by MnP. Using electron spin resonance (ESR) techniques, it was found that the glutathionyl free radical (GS*) was produced through the oxidation of GSH by MnP in the presence as well as in the absence of Mn(II). GS* was also generated through the direct oxidation of GSH by Mn(III). Our results strongly suggest the involvement of GS* in the GSH-mediated mineralization of 2-A-4,6-DNT by MnP.
DOI: 10.1007/s002530000436
PubMed: 11131391
Affiliations:
- Belgique
- Province du Brabant wallon, Région wallonne
- Louvain-la-Neuve
- Université catholique de Louvain
Links toward previous steps (curation, corpus...)
Le document en format XML
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<author><name sortKey="Van Aken, B" sort="Van Aken, B" uniqKey="Van Aken B" first="B" last="Van Aken">B. Van Aken</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aniline Compounds (metabolism)</term>
<term>Biodegradation, Environmental (MeSH)</term>
<term>Electron Spin Resonance Spectroscopy (MeSH)</term>
<term>Free Radicals (MeSH)</term>
<term>Glutathione (metabolism)</term>
<term>Manganese (metabolism)</term>
<term>Oxidation-Reduction (MeSH)</term>
<term>Peroxidases (metabolism)</term>
<term>Phanerochaete (enzymology)</term>
<term>Spin Labels (MeSH)</term>
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<term>Glutathion (métabolisme)</term>
<term>Manganèse (métabolisme)</term>
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<term>Oxydoréduction (MeSH)</term>
<term>Peroxidases (métabolisme)</term>
<term>Phanerochaete (enzymologie)</term>
<term>Radicaux libres (MeSH)</term>
<term>Spectroscopie de résonance de spin électronique (MeSH)</term>
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<term>Glutathione</term>
<term>Manganese</term>
<term>Peroxidases</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term>
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<term>Spin Labels</term>
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<term>Marqueurs de spin</term>
<term>Oxydoréduction</term>
<term>Radicaux libres</term>
<term>Spectroscopie de résonance de spin électronique</term>
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<front><div type="abstract" xml:lang="en">Manganese-dependent peroxidase (MnP) H5 from the white-rot fungus Phanerochaete chrysosporium, in the presence of either Mn(II) (10 mM) or GSH (10 mM). was able to mineralize 14C-U-ring-labeled 2-amino-4,6-dinitrotoluene (2-A-4,6-DNT) up to 29% in 12 days. When both Mn(II) and GSH were present, the mineralization extent reached 82%. On the other hand, no significant mineralization was observed in the absence of both Mn(II) and GSH, suggesting the requirement of a mediator [either Mn(II) or GSH] for the degradation of 2-A-4,6-DNT by MnP. Using electron spin resonance (ESR) techniques, it was found that the glutathionyl free radical (GS*) was produced through the oxidation of GSH by MnP in the presence as well as in the absence of Mn(II). GS* was also generated through the direct oxidation of GSH by Mn(III). Our results strongly suggest the involvement of GS* in the GSH-mediated mineralization of 2-A-4,6-DNT by MnP.</div>
</front>
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<Abstract><AbstractText>Manganese-dependent peroxidase (MnP) H5 from the white-rot fungus Phanerochaete chrysosporium, in the presence of either Mn(II) (10 mM) or GSH (10 mM). was able to mineralize 14C-U-ring-labeled 2-amino-4,6-dinitrotoluene (2-A-4,6-DNT) up to 29% in 12 days. When both Mn(II) and GSH were present, the mineralization extent reached 82%. On the other hand, no significant mineralization was observed in the absence of both Mn(II) and GSH, suggesting the requirement of a mediator [either Mn(II) or GSH] for the degradation of 2-A-4,6-DNT by MnP. Using electron spin resonance (ESR) techniques, it was found that the glutathionyl free radical (GS*) was produced through the oxidation of GSH by MnP in the presence as well as in the absence of Mn(II). GS* was also generated through the direct oxidation of GSH by Mn(III). Our results strongly suggest the involvement of GS* in the GSH-mediated mineralization of 2-A-4,6-DNT by MnP.</AbstractText>
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