Laccase production by Phanerochaete chrysosporium--an artefact caused by Mn(III)?
Identifieur interne : 000A35 ( Main/Exploration ); précédent : 000A34; suivant : 000A36Laccase production by Phanerochaete chrysosporium--an artefact caused by Mn(III)?
Auteurs : H. Podgornik [Slovénie] ; M. Stegu ; E. Zibert ; A. PerdihSource :
- Letters in applied microbiology [ 0266-8254 ] ; 2001.
Descripteurs français
- KwdFr :
- Acides sulfoniques (métabolisme), Benzothiazoles (MeSH), Faux positifs (MeSH), Laccase (MeSH), Manganèse (pharmacologie), Oxidoreductases (biosynthèse), Oxydoréduction (MeSH), Phanerochaete (effets des médicaments et des substances chimiques), Phanerochaete (enzymologie), Phanerochaete (métabolisme).
- MESH :
- biosynthèse : Oxidoreductases.
- effets des médicaments et des substances chimiques : Phanerochaete.
- enzymologie : Phanerochaete.
- métabolisme : Acides sulfoniques, Phanerochaete.
- pharmacologie : Manganèse.
- Benzothiazoles, Faux positifs, Laccase, Oxydoréduction.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Oxidoreductases.
- chemical , metabolism : Sulfonic Acids.
- chemical , pharmacology : Manganese.
- chemical : Benzothiazoles, Laccase.
- drug effects : Phanerochaete.
- enzymology : Phanerochaete.
- metabolism : Phanerochaete.
- False Positive Reactions, Oxidation-Reduction.
Abstract
AIMS
The possibility of laccase production by Phanerochaete chrysosporium was studied.
METHODS AND RESULTS
A relatively high initial Mn(II) concentration (1-4 mM) in the growth medium leads to the development of reddish-brown coloration and intensive oxidation of 2.2'-azino-bis(3-etilbenz-tiazolin-6-sulfonate) (ABTS). The peak of ABTS oxidation was obtained approximately 1 day after the peak of MnP activity.
CONCLUSION
ABTS oxidation was not caused by manganese peroxidase (MnP) nor by laccase but was the consequence of the action of Mn(III) which was stabilised in the growth medium. Decomposition of the complex took place after the biomass was removed from the growth medium and especially after the aeration of the culture was interrupted.
SIGNIFICANCE AND IMPACT OF THE STUDY
Mn(III) seems to be the cause of false positive laccase reactions. More reliable data on MnP activity can be obtained if the complex is decomposed by the fungus before MnP activity is measured in the medium.
DOI: 10.1046/j.1472-765x.2001.00932.x
PubMed: 11412353
Affiliations:
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Zibert</name></author><author><name sortKey="Perdih, A" sort="Perdih, A" uniqKey="Perdih A" first="A" last="Perdih">A. Perdih</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11412353</idno><idno type="pmid">11412353</idno><idno type="doi">10.1046/j.1472-765x.2001.00932.x</idno><idno type="wicri:Area/Main/Corpus">000A47</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000A47</idno><idno type="wicri:Area/Main/Curation">000A47</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000A47</idno><idno type="wicri:Area/Main/Exploration">000A47</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Laccase production by Phanerochaete chrysosporium--an artefact caused by Mn(III)?</title><author><name sortKey="Podgornik, H" sort="Podgornik, H" uniqKey="Podgornik H" first="H" last="Podgornik">H. Podgornik</name><affiliation wicri:level="1"><nlm:affiliation>University of Ljubljana, Faculty of Chemistry and Chemical Technology, Ljubljana, Slovenia. helena.podgornik@uni-lj.si</nlm:affiliation><country xml:lang="fr">Slovénie</country><wicri:regionArea>University of Ljubljana, Faculty of Chemistry and Chemical Technology, Ljubljana</wicri:regionArea><wicri:noRegion>Ljubljana</wicri:noRegion></affiliation></author><author><name sortKey="Stegu, M" sort="Stegu, M" uniqKey="Stegu M" first="M" last="Stegu">M. Stegu</name></author><author><name sortKey="Zibert, E" sort="Zibert, E" uniqKey="Zibert E" first="E" last="Zibert">E. Zibert</name></author><author><name sortKey="Perdih, A" sort="Perdih, A" uniqKey="Perdih A" first="A" last="Perdih">A. 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The peak of ABTS oxidation was obtained approximately 1 day after the peak of MnP activity.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>ABTS oxidation was not caused by manganese peroxidase (MnP) nor by laccase but was the consequence of the action of Mn(III) which was stabilised in the growth medium. Decomposition of the complex took place after the biomass was removed from the growth medium and especially after the aeration of the culture was interrupted.</p></div><div type="abstract" xml:lang="en"><p><b>SIGNIFICANCE AND IMPACT OF THE STUDY</b></p><p>Mn(III) seems to be the cause of false positive laccase reactions. More reliable data on MnP activity can be obtained if the complex is decomposed by the fungus before MnP activity is measured in the medium.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11412353</PMID><DateCompleted><Year>2001</Year><Month>07</Month><Day>19</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0266-8254</ISSN><JournalIssue CitedMedium="Print"><Volume>32</Volume><Issue>6</Issue><PubDate><Year>2001</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Letters in applied microbiology</Title><ISOAbbreviation>Lett Appl Microbiol</ISOAbbreviation></Journal><ArticleTitle>Laccase production by Phanerochaete chrysosporium--an artefact caused by Mn(III)?</ArticleTitle><Pagination><MedlinePgn>407-11</MedlinePgn></Pagination><Abstract><AbstractText Label="AIMS" NlmCategory="OBJECTIVE">The possibility of laccase production by Phanerochaete chrysosporium was studied.</AbstractText><AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">A relatively high initial Mn(II) concentration (1-4 mM) in the growth medium leads to the development of reddish-brown coloration and intensive oxidation of 2.2'-azino-bis(3-etilbenz-tiazolin-6-sulfonate) (ABTS). The peak of ABTS oxidation was obtained approximately 1 day after the peak of MnP activity.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">ABTS oxidation was not caused by manganese peroxidase (MnP) nor by laccase but was the consequence of the action of Mn(III) which was stabilised in the growth medium. Decomposition of the complex took place after the biomass was removed from the growth medium and especially after the aeration of the culture was interrupted.</AbstractText><AbstractText Label="SIGNIFICANCE AND IMPACT OF THE STUDY" NlmCategory="CONCLUSIONS">Mn(III) seems to be the cause of false positive laccase reactions. More reliable data on MnP activity can be obtained if the complex is decomposed by the fungus before MnP activity is measured in the medium.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Podgornik</LastName><ForeName>H</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>University of Ljubljana, Faculty of Chemistry and Chemical Technology, Ljubljana, Slovenia. helena.podgornik@uni-lj.si</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stegu</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Zibert</LastName><ForeName>E</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Perdih</LastName><ForeName>A</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Lett Appl Microbiol</MedlineTA><NlmUniqueID>8510094</NlmUniqueID><ISSNLinking>0266-8254</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D052160">Benzothiazoles</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013451">Sulfonic Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>28752-68-3</RegistryNumber><NameOfSubstance UI="C002502">2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>42Z2K6ZL8P</RegistryNumber><NameOfSubstance UI="D008345">Manganese</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D010088">Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber><NameOfSubstance UI="D042845">Laccase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D052160" MajorTopicYN="N">Benzothiazoles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005189" MajorTopicYN="N">False Positive Reactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008345" MajorTopicYN="N">Manganese</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010088" MajorTopicYN="N">Oxidoreductases</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013451" MajorTopicYN="N">Sulfonic Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>6</Month><Day>20</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>7</Month><Day>20</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>6</Month><Day>20</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11412353</ArticleId><ArticleId IdType="pii">lam932</ArticleId><ArticleId IdType="doi">10.1046/j.1472-765x.2001.00932.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Slovénie</li></country></list><tree><noCountry><name sortKey="Perdih, A" sort="Perdih, A" uniqKey="Perdih A" first="A" last="Perdih">A. Perdih</name><name sortKey="Stegu, M" sort="Stegu, M" uniqKey="Stegu M" first="M" last="Stegu">M. Stegu</name><name sortKey="Zibert, E" sort="Zibert, E" uniqKey="Zibert E" first="E" last="Zibert">E. Zibert</name></noCountry><country name="Slovénie"><noRegion><name sortKey="Podgornik, H" sort="Podgornik, H" uniqKey="Podgornik H" first="H" last="Podgornik">H. Podgornik</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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