Probing molecular interactions and mechanical properties of microbial cell surfaces by atomic force microscopy.
Identifieur interne : 000A30 ( Main/Exploration ); précédent : 000A29; suivant : 000A31Probing molecular interactions and mechanical properties of microbial cell surfaces by atomic force microscopy.
Auteurs : Y F Dufrêne [Belgique] ; Boonaert CJP ; H C Van Der Mei ; H J Busscher ; P G RouxhetSource :
- Ultramicroscopy [ 0304-3991 ] ; 2001.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Double couche lipidique.
- méthodes : Microscopie à force atomique.
- physiologie : Adhérence cellulaire, Bactéries à Gram positif, Phanerochaete, Spores fongiques.
- Agrégation cellulaire, Propriétés de surface.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Lipid Bilayers.
- methods : Microscopy, Atomic Force.
- physiology : Cell Adhesion, Gram-Positive Bacteria, Phanerochaete, Spores, Fungal.
- Cell Aggregation, Surface Properties.
Abstract
Knowledge of the surface properties of microbial cells is a key to gain a detailed understanding of their functions in the natural environment and to efficiently exploit them in biotechnological processes. In this paper, we present force-distance curves recorded, by atomic force microscopy (AFM) in aqueous solutions, on various microbial samples: reconstituted S-layers, whole fungal spores and several bacterial strains. The approach and retraction curves exhibited important differences--depending on the type of microorganism, on the physiological state (dormancy versus germination) and on the environmental conditions (ionic strength)--which were shown to reflect differences in long-range surface forces, adhesion forces and mechanical properties. These data illustrate the great potential of AFM force measurements to elucidate the physical properties of microbial cells and to understand, at the molecular level, biointerfacial phenomena such as cell adhesion and cell aggregation.
DOI: 10.1016/s0304-3991(00)00079-6
PubMed: 11215613
Affiliations:
- Belgique
- Province du Brabant wallon, Région wallonne
- Louvain-la-Neuve
- Université catholique de Louvain
Links toward previous steps (curation, corpus...)
Le document en format XML
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uniqKey="Boonaert Cjp" last="Boonaert Cjp">Boonaert CJP</name></author><author><name sortKey="Van Der Mei, H C" sort="Van Der Mei, H C" uniqKey="Van Der Mei H" first="H C" last="Van Der Mei">H C Van Der Mei</name></author><author><name sortKey="Busscher, H J" sort="Busscher, H J" uniqKey="Busscher H" first="H J" last="Busscher">H J Busscher</name></author><author><name sortKey="Rouxhet, P G" sort="Rouxhet, P G" uniqKey="Rouxhet P" first="P G" last="Rouxhet">P G Rouxhet</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11215613</idno><idno type="pmid">11215613</idno><idno type="doi">10.1016/s0304-3991(00)00079-6</idno><idno type="wicri:Area/Main/Corpus">000A67</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000A67</idno><idno type="wicri:Area/Main/Curation">000A67</idno><idno type="wicri:explorRef" wicri:stream="Main" 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Bilayers (metabolism)</term><term>Microscopy, Atomic Force (methods)</term><term>Phanerochaete (physiology)</term><term>Spores, Fungal (physiology)</term><term>Surface Properties (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adhérence cellulaire (physiologie)</term><term>Agrégation cellulaire (MeSH)</term><term>Bactéries à Gram positif (physiologie)</term><term>Double couche lipidique (métabolisme)</term><term>Microscopie à force atomique (méthodes)</term><term>Phanerochaete (physiologie)</term><term>Propriétés de surface (MeSH)</term><term>Spores fongiques (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lipid Bilayers</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microscopy, Atomic Force</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Double couche lipidique</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Microscopie à force atomique</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Adhérence cellulaire</term><term>Bactéries à Gram positif</term><term>Phanerochaete</term><term>Spores fongiques</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Adhesion</term><term>Gram-Positive Bacteria</term><term>Phanerochaete</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Aggregation</term><term>Surface Properties</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Agrégation cellulaire</term><term>Propriétés de surface</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Knowledge of the surface properties of microbial cells is a key to gain a detailed understanding of their functions in the natural environment and to efficiently exploit them in biotechnological processes. In this paper, we present force-distance curves recorded, by atomic force microscopy (AFM) in aqueous solutions, on various microbial samples: reconstituted S-layers, whole fungal spores and several bacterial strains. The approach and retraction curves exhibited important differences--depending on the type of microorganism, on the physiological state (dormancy versus germination) and on the environmental conditions (ionic strength)--which were shown to reflect differences in long-range surface forces, adhesion forces and mechanical properties. These data illustrate the great potential of AFM force measurements to elucidate the physical properties of microbial cells and to understand, at the molecular level, biointerfacial phenomena such as cell adhesion and cell aggregation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11215613</PMID><DateCompleted><Year>2001</Year><Month>06</Month><Day>14</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0304-3991</ISSN><JournalIssue CitedMedium="Print"><Volume>86</Volume><Issue>1-2</Issue><PubDate><Year>2001</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Ultramicroscopy</Title><ISOAbbreviation>Ultramicroscopy</ISOAbbreviation></Journal><ArticleTitle>Probing molecular interactions and mechanical properties of microbial cell surfaces by atomic force microscopy.</ArticleTitle><Pagination><MedlinePgn>113-20</MedlinePgn></Pagination><Abstract><AbstractText>Knowledge of the surface properties of microbial cells is a key to gain a detailed understanding of their functions in the natural environment and to efficiently exploit them in biotechnological processes. In this paper, we present force-distance curves recorded, by atomic force microscopy (AFM) in aqueous solutions, on various microbial samples: reconstituted S-layers, whole fungal spores and several bacterial strains. The approach and retraction curves exhibited important differences--depending on the type of microorganism, on the physiological state (dormancy versus germination) and on the environmental conditions (ionic strength)--which were shown to reflect differences in long-range surface forces, adhesion forces and mechanical properties. These data illustrate the great potential of AFM force measurements to elucidate the physical properties of microbial cells and to understand, at the molecular level, biointerfacial phenomena such as cell adhesion and cell aggregation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dufrêne</LastName><ForeName>Y F</ForeName><Initials>YF</Initials><AffiliationInfo><Affiliation>Unité de chimie des interfaces, Université catholique de Louvain, Louvain-la-Neuve, Belgium. dufrene@cifa.ucl.ac.be</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Boonaert CJP</LastName></Author><Author ValidYN="Y"><LastName>van der Mei</LastName><ForeName>H C</ForeName><Initials>HC</Initials></Author><Author ValidYN="Y"><LastName>Busscher</LastName><ForeName>H J</ForeName><Initials>HJ</Initials></Author><Author ValidYN="Y"><LastName>Rouxhet</LastName><ForeName>P G</ForeName><Initials>PG</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Ultramicroscopy</MedlineTA><NlmUniqueID>7513702</NlmUniqueID><ISSNLinking>0304-3991</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008051">Lipid Bilayers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002448" MajorTopicYN="N">Cell Adhesion</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002449" MajorTopicYN="N">Cell Aggregation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006094" MajorTopicYN="N">Gram-Positive Bacteria</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008051" MajorTopicYN="N">Lipid Bilayers</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018625" MajorTopicYN="N">Microscopy, Atomic Force</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013172" MajorTopicYN="N">Spores, Fungal</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013499" MajorTopicYN="N">Surface Properties</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>2</Month><Day>24</Day><Hour>12</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>6</Month><Day>23</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>2</Month><Day>24</Day><Hour>12</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11215613</ArticleId><ArticleId IdType="pii">S0304-3991(00)00079-6</ArticleId><ArticleId IdType="doi">10.1016/s0304-3991(00)00079-6</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province du Brabant wallon</li><li>Région wallonne</li></region><settlement><li>Louvain-la-Neuve</li></settlement><orgName><li>Université catholique de Louvain</li></orgName></list><tree><noCountry><name sortKey="Boonaert Cjp" sort="Boonaert Cjp" uniqKey="Boonaert Cjp" last="Boonaert Cjp">Boonaert CJP</name><name sortKey="Busscher, H J" sort="Busscher, H J" uniqKey="Busscher H" first="H J" last="Busscher">H J Busscher</name><name sortKey="Rouxhet, P G" sort="Rouxhet, P G" uniqKey="Rouxhet P" first="P G" last="Rouxhet">P G Rouxhet</name><name sortKey="Van Der Mei, H C" sort="Van Der Mei, H C" uniqKey="Van Der Mei H" first="H C" last="Van Der Mei">H C Van Der Mei</name></noCountry><country name="Belgique"><region name="Région wallonne"><name sortKey="Dufrene, Y F" sort="Dufrene, Y F" uniqKey="Dufrene Y" first="Y F" last="Dufrêne">Y F Dufrêne</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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