Serveur d'exploration sur le phanerochaete

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Production of manganese-dependent peroxidase in a new solid-state bioreactor by Phanerochaete chrysosporium grown on wood shavings. Application to the decolorization of synthetic dyes.

Identifieur interne : 000981 ( Main/Exploration ); précédent : 000980; suivant : 000982

Production of manganese-dependent peroxidase in a new solid-state bioreactor by Phanerochaete chrysosporium grown on wood shavings. Application to the decolorization of synthetic dyes.

Auteurs : S. Rodríguez Couto [Espagne] ; A. Domínguez ; A. Sanromán

Source :

RBID : pubmed:12422521

Descripteurs français

English descriptors

Abstract

The production of manganese-dependent peroxidase (MnP) by Phanerochaete chrysosporium in a new solid-state bioreactor, the immersion bioreactor, operating with lignocellulosic waste, such as wood shavings, was investigated. Maximum MnP and lignin peroxidase (LiP) activity of 13.4 and 8.48 mukat/L were obtained, respectively. The in vitro decolorization of several synthetic dyes by the extracellular liquid produced in the above-mentioned bioreactor (containing mainly MnP) was carried out and its degrading ability was assessed. The highest decolorization was reached with Indigo Carmine (98%) followed by Bromophenol Blue (56%) and Methyl Orange (36%), whereas Gentian Violet was hardly decolorized (6%).

DOI: 10.1007/BF02818701
PubMed: 12422521


Affiliations:


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Le document en format XML

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<name sortKey="Rodriguez Couto, S" sort="Rodriguez Couto, S" uniqKey="Rodriguez Couto S" first="S" last="Rodríguez Couto">S. Rodríguez Couto</name>
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<term>Peroxidases (biosynthesis)</term>
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<term>Phanerochaete (enzymology)</term>
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<term>Agents colorants (métabolisme)</term>
<term>Bioréacteurs (MeSH)</term>
<term>Bois (MeSH)</term>
<term>Couleur (MeSH)</term>
<term>Dépollution biologique de l'environnement (MeSH)</term>
<term>Milieux de culture (MeSH)</term>
<term>Peroxidases (biosynthèse)</term>
<term>Peroxidases (métabolisme)</term>
<term>Phanerochaete (croissance et développement)</term>
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<div type="abstract" xml:lang="en">The production of manganese-dependent peroxidase (MnP) by Phanerochaete chrysosporium in a new solid-state bioreactor, the immersion bioreactor, operating with lignocellulosic waste, such as wood shavings, was investigated. Maximum MnP and lignin peroxidase (LiP) activity of 13.4 and 8.48 mukat/L were obtained, respectively. The in vitro decolorization of several synthetic dyes by the extracellular liquid produced in the above-mentioned bioreactor (containing mainly MnP) was carried out and its degrading ability was assessed. The highest decolorization was reached with Indigo Carmine (98%) followed by Bromophenol Blue (56%) and Methyl Orange (36%), whereas Gentian Violet was hardly decolorized (6%).</div>
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