PhanerochaeteV1, Main, Exploration, bibRecord, 000876

Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Pichia methanolica.

Identifieur interne : 000876 ( Main/Exploration ); précédent : 000875; suivant : 000877

Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Pichia methanolica.

Auteurs : Haikuan Wang [République populaire de Chine] ; Fuping Lu ; Yafan Sun ; Lianxiang Du

Source :

Biotechnology letters [ 0141-5492 ] ; 2004.

RBID : pubmed:15604798

Descripteurs français

KwdFr :
- Activation enzymatique (MeSH), Clonage moléculaire (méthodes), Masse moléculaire (MeSH), Peroxidases (biosynthèse), Peroxidases (composition chimique), Peroxidases (génétique), Phanerochaete (enzymologie), Phanerochaete (génétique), Pichia (enzymologie), Pichia (génétique), Protéines recombinantes (biosynthèse), Protéines recombinantes (composition chimique).
MESH :
- biosynthèse : Peroxidases, Protéines recombinantes.
- composition chimique : Peroxidases, Protéines recombinantes.
- enzymologie : Phanerochaete, Pichia.
- génétique : Peroxidases, Phanerochaete, Pichia.
- méthodes : Clonage moléculaire.
- Activation enzymatique, Masse moléculaire.

English descriptors

KwdEn :
- Cloning, Molecular (methods), Enzyme Activation (MeSH), Molecular Weight (MeSH), Peroxidases (biosynthesis), Peroxidases (chemistry), Peroxidases (genetics), Phanerochaete (enzymology), Phanerochaete (genetics), Pichia (enzymology), Pichia (genetics), Recombinant Proteins (biosynthesis), Recombinant Proteins (chemistry).
MESH :
- chemical , biosynthesis : Peroxidases, Recombinant Proteins.
- chemical , chemistry : Peroxidases, Recombinant Proteins.
- chemical , genetics : Peroxidases.
- enzymology : Phanerochaete, Pichia.
- genetics : Phanerochaete, Pichia.
- methods : Cloning, Molecular.
- Enzyme Activation, Molecular Weight.

Abstract

The cDNA encoding for lignin peroxidase of Phanerochaete chrysosporium was expressed in the Pichia methanolica under the control of the alcohol oxidase (AUG1) promoter which was followed by either the lignin peroxidase leader peptide of Phanerochaete chrysosporium or the Saccharomyces cerevisiae alpha-factor signal peptide. Both peptides efficiently directed the secretion of lignin peroxidase from the recombinant yeast cell. The extracellular lignin peroxidase activity in two recombinants was 932 U l(-1) and 1933 U l(-1). The purity of the recombinant product was confirmed by SDS-PAGE.

DOI: 10.1023/B:BILE.0000045654.66689.b4
PubMed: 15604798

Affiliations:

République populaire de Chine

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Le document en format XML

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<author><name sortKey="Wang, Haikuan" sort="Wang, Haikuan" uniqKey="Wang H" first="Haikuan" last="Wang">Haikuan Wang</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Food Science and Biology Engineering, Tianjin Science and Technology University, P.R. China.</nlm:affiliation>
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<term>Peroxidases (chemistry)</term>
<term>Peroxidases (genetics)</term>
<term>Phanerochaete (enzymology)</term>
<term>Phanerochaete (genetics)</term>
<term>Pichia (enzymology)</term>
<term>Pichia (genetics)</term>
<term>Recombinant Proteins (biosynthesis)</term>
<term>Recombinant Proteins (chemistry)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Activation enzymatique (MeSH)</term>
<term>Clonage moléculaire (méthodes)</term>
<term>Masse moléculaire (MeSH)</term>
<term>Peroxidases (biosynthèse)</term>
<term>Peroxidases (composition chimique)</term>
<term>Peroxidases (génétique)</term>
<term>Phanerochaete (enzymologie)</term>
<term>Phanerochaete (génétique)</term>
<term>Pichia (enzymologie)</term>
<term>Pichia (génétique)</term>
<term>Protéines recombinantes (biosynthèse)</term>
<term>Protéines recombinantes (composition chimique)</term>
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<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Peroxidases</term>
<term>Recombinant Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Peroxidases</term>
<term>Recombinant Proteins</term>
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<front><div type="abstract" xml:lang="en">The cDNA encoding for lignin peroxidase of Phanerochaete chrysosporium was expressed in the Pichia methanolica under the control of the alcohol oxidase (AUG1) promoter which was followed by either the lignin peroxidase leader peptide of Phanerochaete chrysosporium or the Saccharomyces cerevisiae alpha-factor signal peptide. Both peptides efficiently directed the secretion of lignin peroxidase from the recombinant yeast cell. The extracellular lignin peroxidase activity in two recombinants was 932 U l(-1) and 1933 U l(-1). The purity of the recombinant product was confirmed by SDS-PAGE.</div>
</front>
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<Month>Oct</Month>
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<Title>Biotechnology letters</Title>
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<Abstract><AbstractText>The cDNA encoding for lignin peroxidase of Phanerochaete chrysosporium was expressed in the Pichia methanolica under the control of the alcohol oxidase (AUG1) promoter which was followed by either the lignin peroxidase leader peptide of Phanerochaete chrysosporium or the Saccharomyces cerevisiae alpha-factor signal peptide. Both peptides efficiently directed the secretion of lignin peroxidase from the recombinant yeast cell. The extracellular lignin peroxidase activity in two recombinants was 932 U l(-1) and 1933 U l(-1). The purity of the recombinant product was confirmed by SDS-PAGE.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
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Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Pichia methanolica.

Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Pichia methanolica.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki