Long serial analysis of gene expression for transcriptome profiling during the initiation of ligninolytic enzymes production in Phanerochaete chrysosporium.
Identifieur interne : 000725 ( Main/Exploration ); précédent : 000724; suivant : 000726Long serial analysis of gene expression for transcriptome profiling during the initiation of ligninolytic enzymes production in Phanerochaete chrysosporium.
Auteurs : Masahiko Minami [Japon] ; Orie Kureha ; Mari Mori ; Hisatoshi Kamitsuji ; Kazumi Suzuki ; Toshikazu IrieSource :
- Applied microbiology and biotechnology [ 0175-7598 ] ; 2007.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes (méthodes), Banque de gènes (MeSH), Gènes fongiques (MeSH), Lignine (métabolisme), Peroxidases (génétique), Peroxidases (métabolisme), Phanerochaete (enzymologie), Phanerochaete (génétique), Phanerochaete (métabolisme), RT-PCR (MeSH), Transcription génétique (MeSH).
- MESH :
- enzymologie : Phanerochaete.
- génétique : Peroxidases, Phanerochaete.
- métabolisme : Lignine, Peroxidases, Phanerochaete.
- méthodes : Analyse de profil d'expression de gènes.
- Banque de gènes, Gènes fongiques, RT-PCR, Transcription génétique.
English descriptors
- KwdEn :
- Gene Expression Profiling (methods), Gene Library (MeSH), Genes, Fungal (MeSH), Lignin (metabolism), Peroxidases (genetics), Peroxidases (metabolism), Phanerochaete (enzymology), Phanerochaete (genetics), Phanerochaete (metabolism), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Transcription, Genetic (MeSH).
- MESH :
- chemical , genetics : Peroxidases.
- chemical , metabolism : Lignin, Peroxidases.
- enzymology : Phanerochaete.
- genetics : Phanerochaete.
- metabolism : Phanerochaete.
- methods : Gene Expression Profiling.
- Gene Library, Genes, Fungal, Reverse Transcriptase Polymerase Chain Reaction, Transcription, Genetic.
Abstract
To analyze the transcriptome profile during the initiation of manganese peroxidase (MnP) and lignin peroxidase (LiP) production in Phanerochaete chrysosporium, we constructed long serial analysis of gene expression (LongSAGE) libraries. A total of 13,666 tags (the number of cumulative counted tags) that included 6,945 unique tags (the number of distinct tags) were isolated from the day-3 culture, which just started the enzymes production and was 24 h after veratryl alcohol addition and oxygen-purge into the culture (day-2 culture). A total of 12,402 tags that included 6,396 unique tags were isolated from the day-2 culture, in which the activity of enzymes is not detected. The comparison of the two libraries suggested that 38 genes showed significant (p < or = 0.01) fourfold or greater upregulation; this included the MnP gene (mnp2, mnp3) and LiP H8 gene. On the other hand, 43 genes showed significant (p < or = 0.01) fourfold or greater downregulation. This LongSAGE analysis found many new candidate genes regulating the enzymes production.
DOI: 10.1007/s00253-007-0850-y
PubMed: 17308906
Affiliations:
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uniqKey="Mori M" first="Mari" last="Mori">Mari Mori</name></author><author><name sortKey="Kamitsuji, Hisatoshi" sort="Kamitsuji, Hisatoshi" uniqKey="Kamitsuji H" first="Hisatoshi" last="Kamitsuji">Hisatoshi Kamitsuji</name></author><author><name sortKey="Suzuki, Kazumi" sort="Suzuki, Kazumi" uniqKey="Suzuki K" first="Kazumi" last="Suzuki">Kazumi Suzuki</name></author><author><name sortKey="Irie, Toshikazu" sort="Irie, Toshikazu" uniqKey="Irie T" first="Toshikazu" last="Irie">Toshikazu Irie</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17308906</idno><idno type="pmid">17308906</idno><idno type="doi">10.1007/s00253-007-0850-y</idno><idno type="wicri:Area/Main/Corpus">000747</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000747</idno><idno type="wicri:Area/Main/Curation">000747</idno><idno type="wicri:explorRef" wicri:stream="Main" 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last="Kureha">Orie Kureha</name></author><author><name sortKey="Mori, Mari" sort="Mori, Mari" uniqKey="Mori M" first="Mari" last="Mori">Mari Mori</name></author><author><name sortKey="Kamitsuji, Hisatoshi" sort="Kamitsuji, Hisatoshi" uniqKey="Kamitsuji H" first="Hisatoshi" last="Kamitsuji">Hisatoshi Kamitsuji</name></author><author><name sortKey="Suzuki, Kazumi" sort="Suzuki, Kazumi" uniqKey="Suzuki K" first="Kazumi" last="Suzuki">Kazumi Suzuki</name></author><author><name sortKey="Irie, Toshikazu" sort="Irie, Toshikazu" uniqKey="Irie T" first="Toshikazu" last="Irie">Toshikazu Irie</name></author></analytic><series><title level="j">Applied microbiology and biotechnology</title><idno type="ISSN">0175-7598</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene Expression Profiling (methods)</term><term>Gene Library (MeSH)</term><term>Genes, Fungal (MeSH)</term><term>Lignin (metabolism)</term><term>Peroxidases (genetics)</term><term>Peroxidases (metabolism)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (genetics)</term><term>Phanerochaete (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Transcription, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (méthodes)</term><term>Banque de gènes (MeSH)</term><term>Gènes fongiques (MeSH)</term><term>Lignine (métabolisme)</term><term>Peroxidases (génétique)</term><term>Peroxidases (métabolisme)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (génétique)</term><term>Phanerochaete (métabolisme)</term><term>RT-PCR (MeSH)</term><term>Transcription génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peroxidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Peroxidases</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Gene Expression Profiling</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lignine</term><term>Peroxidases</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Library</term><term>Genes, Fungal</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Banque de gènes</term><term>Gènes fongiques</term><term>RT-PCR</term><term>Transcription génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To analyze the transcriptome profile during the initiation of manganese peroxidase (MnP) and lignin peroxidase (LiP) production in Phanerochaete chrysosporium, we constructed long serial analysis of gene expression (LongSAGE) libraries. A total of 13,666 tags (the number of cumulative counted tags) that included 6,945 unique tags (the number of distinct tags) were isolated from the day-3 culture, which just started the enzymes production and was 24 h after veratryl alcohol addition and oxygen-purge into the culture (day-2 culture). A total of 12,402 tags that included 6,396 unique tags were isolated from the day-2 culture, in which the activity of enzymes is not detected. The comparison of the two libraries suggested that 38 genes showed significant (p < or = 0.01) fourfold or greater upregulation; this included the MnP gene (mnp2, mnp3) and LiP H8 gene. On the other hand, 43 genes showed significant (p < or = 0.01) fourfold or greater downregulation. This LongSAGE analysis found many new candidate genes regulating the enzymes production.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17308906</PMID><DateCompleted><Year>2007</Year><Month>09</Month><Day>04</Day></DateCompleted><DateRevised><Year>2007</Year><Month>05</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0175-7598</ISSN><JournalIssue CitedMedium="Print"><Volume>75</Volume><Issue>3</Issue><PubDate><Year>2007</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Long serial analysis of gene expression for transcriptome profiling during the initiation of ligninolytic enzymes production in Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>609-18</MedlinePgn></Pagination><Abstract><AbstractText>To analyze the transcriptome profile during the initiation of manganese peroxidase (MnP) and lignin peroxidase (LiP) production in Phanerochaete chrysosporium, we constructed long serial analysis of gene expression (LongSAGE) libraries. A total of 13,666 tags (the number of cumulative counted tags) that included 6,945 unique tags (the number of distinct tags) were isolated from the day-3 culture, which just started the enzymes production and was 24 h after veratryl alcohol addition and oxygen-purge into the culture (day-2 culture). A total of 12,402 tags that included 6,396 unique tags were isolated from the day-2 culture, in which the activity of enzymes is not detected. The comparison of the two libraries suggested that 38 genes showed significant (p < or = 0.01) fourfold or greater upregulation; this included the MnP gene (mnp2, mnp3) and LiP H8 gene. On the other hand, 43 genes showed significant (p < or = 0.01) fourfold or greater downregulation. This LongSAGE analysis found many new candidate genes regulating the enzymes production.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Minami</LastName><ForeName>Masahiko</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Environmental Science, The University of Shiga Prefecture, 2500 Hassaka-cho, Hikone, Shiga, 522-8533, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kureha</LastName><ForeName>Orie</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Mori</LastName><ForeName>Mari</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Kamitsuji</LastName><ForeName>Hisatoshi</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Suzuki</LastName><ForeName>Kazumi</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Irie</LastName><ForeName>Toshikazu</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>02</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Appl Microbiol Biotechnol</MedlineTA><NlmUniqueID>8406612</NlmUniqueID><ISSNLinking>0175-7598</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="C042858">lignin peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" 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PubStatus="pubmed"><Year>2007</Year><Month>2</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>9</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>2</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17308906</ArticleId><ArticleId IdType="doi">10.1007/s00253-007-0850-y</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Irie, Toshikazu" sort="Irie, Toshikazu" uniqKey="Irie T" first="Toshikazu" last="Irie">Toshikazu Irie</name><name sortKey="Kamitsuji, Hisatoshi" sort="Kamitsuji, Hisatoshi" uniqKey="Kamitsuji H" first="Hisatoshi" last="Kamitsuji">Hisatoshi Kamitsuji</name><name sortKey="Kureha, Orie" sort="Kureha, Orie" uniqKey="Kureha O" first="Orie" last="Kureha">Orie Kureha</name><name sortKey="Mori, Mari" sort="Mori, Mari" uniqKey="Mori M" first="Mari" last="Mori">Mari Mori</name><name sortKey="Suzuki, Kazumi" sort="Suzuki, Kazumi" uniqKey="Suzuki K" first="Kazumi" last="Suzuki">Kazumi Suzuki</name></noCountry><country name="Japon"><noRegion><name sortKey="Minami, Masahiko" sort="Minami, Masahiko" uniqKey="Minami M" first="Masahiko" last="Minami">Masahiko Minami</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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