The copper-dependent ACE1 transcription factor activates the transcription of the mco1 gene from the basidiomycete Phanerochaete chrysosporium.
Identifieur interne : 000669 ( Main/Exploration ); précédent : 000668; suivant : 000670The copper-dependent ACE1 transcription factor activates the transcription of the mco1 gene from the basidiomycete Phanerochaete chrysosporium.
Auteurs : Paulo Canessa [Chili] ; José Miguel Álvarez [Chili] ; Rubén Polanco [Chili] ; Paulina Bull [Chili] ; Rafael Vicu A [Chili]Source :
- Microbiology (Reading, England) [ 1350-0872 ] ; 2008.
Descripteurs français
- KwdFr :
- Cuivre (métabolisme), Facteurs de transcription (génétique), Oxidoreductases (génétique), Phanerochaete (enzymologie), Phanerochaete (génétique), Phanerochaete (métabolisme), Protéines de liaison à l'ADN (génétique), Protéines fongiques (génétique), RT-PCR (MeSH), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Sites de fixation (génétique), Séquence consensus (génétique), Séquence nucléotidique (MeSH), Test de retard de migration électrophorétique (MeSH), Transcription génétique (MeSH).
- MESH :
- enzymologie : Phanerochaete.
- génétique : Facteurs de transcription, Oxidoreductases, Phanerochaete, Protéines de liaison à l'ADN, Protéines fongiques, Sites de fixation, Séquence consensus.
- métabolisme : Cuivre, Phanerochaete.
- RT-PCR, Régions promotrices (génétique), Régulation de l'expression des gènes, Régulation de l'expression des gènes fongiques, Séquence nucléotidique, Test de retard de migration électrophorétique, Transcription génétique.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Binding Sites (genetics), Consensus Sequence (genetics), Copper (metabolism), DNA-Binding Proteins (genetics), Electrophoretic Mobility Shift Assay (MeSH), Fungal Proteins (genetics), Gene Expression Regulation (MeSH), Gene Expression Regulation, Fungal (MeSH), Oxidoreductases (genetics), Phanerochaete (enzymology), Phanerochaete (genetics), Phanerochaete (metabolism), Promoter Regions, Genetic (MeSH), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Transcription Factors (genetics), Transcription, Genetic (MeSH).
- MESH :
- chemical , genetics : DNA-Binding Proteins, Fungal Proteins, Oxidoreductases, Transcription Factors.
- chemical , metabolism : Copper.
- enzymology : Phanerochaete.
- genetics : Binding Sites, Consensus Sequence, Phanerochaete.
- metabolism : Phanerochaete.
- Base Sequence, Electrophoretic Mobility Shift Assay, Gene Expression Regulation, Gene Expression Regulation, Fungal, Promoter Regions, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Transcription, Genetic.
Abstract
We have previously identified and functionally characterized the transcription factor ACE1 (Pc-ACE1) from Phanerochaete chrysosporium. In Saccharomyces cerevisiae, ACE1 activates the copper-dependent transcription of target genes through a DNA sequence element named ACE. However, the possible target gene(s) of Pc-ACE1 were unknown. An in silico search led to the identification of putative ACE elements in the promoter region of mco1, one of the four clustered genes encoding multicopper oxidases (MCOs) in P. chrysosporium. Since copper exerts an effect at the transcriptional level in MCOs from several organisms, in this work we analysed the effect of copper on transcript levels of the clustered MCO genes from P. chrysosporium, with the exception of the transcriptionally inactive mco3. Copper supplementation of cultures produced an increment in transcripts from genes mco1 and mco2, but not from mco4. Electrophoretic mobility-shift assays revealed that Pc-ACE1 binds specifically to a probe containing one of the putative ACE elements found in the promoter of mco1. In addition, using a cell-free transcription system, we showed that in the presence of cuprous ion, Pc-ACE1 induces activation of the promoter of mco1, but not that of mco2.
DOI: 10.1099/mic.0.2007/013128-0
PubMed: 18227253
Affiliations:
Links toward previous steps (curation, corpus...)
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Milenio de Biología Fundamental y Aplicada, Santiago, Chile.</nlm:affiliation><country xml:lang="fr">Chili</country><wicri:regionArea>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago</wicri:regionArea><wicri:noRegion>Santiago</wicri:noRegion></affiliation></author><author><name sortKey="Alvarez, Jose Miguel" sort="Alvarez, Jose Miguel" uniqKey="Alvarez J" first="José Miguel" last="Álvarez">José Miguel Álvarez</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile.</nlm:affiliation><country xml:lang="fr">Chili</country><wicri:regionArea>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago</wicri:regionArea><wicri:noRegion>Santiago</wicri:noRegion></affiliation></author><author><name sortKey="Polanco, Ruben" sort="Polanco, Ruben" uniqKey="Polanco R" first="Rubén" last="Polanco">Rubén Polanco</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile.</nlm:affiliation><country xml:lang="fr">Chili</country><wicri:regionArea>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago</wicri:regionArea><wicri:noRegion>Santiago</wicri:noRegion></affiliation></author><author><name sortKey="Bull, Paulina" sort="Bull, Paulina" uniqKey="Bull P" first="Paulina" last="Bull">Paulina Bull</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile.</nlm:affiliation><country xml:lang="fr">Chili</country><wicri:regionArea>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago</wicri:regionArea><wicri:noRegion>Santiago</wicri:noRegion></affiliation></author><author><name sortKey="Vicu A, Rafael" sort="Vicu A, Rafael" uniqKey="Vicu A R" first="Rafael" last="Vicu A">Rafael Vicu A</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile.</nlm:affiliation><country xml:lang="fr">Chili</country><wicri:regionArea>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago</wicri:regionArea><wicri:noRegion>Santiago</wicri:noRegion></affiliation></author></analytic><series><title level="j">Microbiology (Reading, England)</title><idno type="ISSN">1350-0872</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Binding Sites (genetics)</term><term>Consensus Sequence (genetics)</term><term>Copper (metabolism)</term><term>DNA-Binding Proteins (genetics)</term><term>Electrophoretic Mobility Shift Assay (MeSH)</term><term>Fungal Proteins (genetics)</term><term>Gene Expression Regulation (MeSH)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Oxidoreductases (genetics)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (genetics)</term><term>Phanerochaete (metabolism)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Transcription Factors (genetics)</term><term>Transcription, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cuivre (métabolisme)</term><term>Facteurs de transcription (génétique)</term><term>Oxidoreductases (génétique)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (génétique)</term><term>Phanerochaete (métabolisme)</term><term>Protéines de liaison à l'ADN (génétique)</term><term>Protéines fongiques (génétique)</term><term>RT-PCR (MeSH)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes (MeSH)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Sites de fixation (génétique)</term><term>Séquence consensus (génétique)</term><term>Séquence nucléotidique (MeSH)</term><term>Test de retard de migration électrophorétique (MeSH)</term><term>Transcription génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA-Binding Proteins</term><term>Fungal Proteins</term><term>Oxidoreductases</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Copper</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Binding Sites</term><term>Consensus Sequence</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de transcription</term><term>Oxidoreductases</term><term>Phanerochaete</term><term>Protéines de liaison à l'ADN</term><term>Protéines fongiques</term><term>Sites de fixation</term><term>Séquence consensus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cuivre</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Electrophoretic Mobility Shift Assay</term><term>Gene Expression Regulation</term><term>Gene Expression Regulation, Fungal</term><term>Promoter Regions, Genetic</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>RT-PCR</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes</term><term>Régulation de l'expression des gènes fongiques</term><term>Séquence nucléotidique</term><term>Test de retard de migration électrophorétique</term><term>Transcription génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have previously identified and functionally characterized the transcription factor ACE1 (Pc-ACE1) from Phanerochaete chrysosporium. In Saccharomyces cerevisiae, ACE1 activates the copper-dependent transcription of target genes through a DNA sequence element named ACE. However, the possible target gene(s) of Pc-ACE1 were unknown. An in silico search led to the identification of putative ACE elements in the promoter region of mco1, one of the four clustered genes encoding multicopper oxidases (MCOs) in P. chrysosporium. Since copper exerts an effect at the transcriptional level in MCOs from several organisms, in this work we analysed the effect of copper on transcript levels of the clustered MCO genes from P. chrysosporium, with the exception of the transcriptionally inactive mco3. Copper supplementation of cultures produced an increment in transcripts from genes mco1 and mco2, but not from mco4. Electrophoretic mobility-shift assays revealed that Pc-ACE1 binds specifically to a probe containing one of the putative ACE elements found in the promoter of mco1. In addition, using a cell-free transcription system, we showed that in the presence of cuprous ion, Pc-ACE1 induces activation of the promoter of mco1, but not that of mco2.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18227253</PMID><DateCompleted><Year>2008</Year><Month>04</Month><Day>03</Day></DateCompleted><DateRevised><Year>2020</Year><Month>08</Month><Day>26</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1350-0872</ISSN><JournalIssue CitedMedium="Print"><Volume>154</Volume><Issue>Pt 2</Issue><PubDate><Year>2008</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Microbiology (Reading, England)</Title><ISOAbbreviation>Microbiology (Reading)</ISOAbbreviation></Journal><ArticleTitle>The copper-dependent ACE1 transcription factor activates the transcription of the mco1 gene from the basidiomycete Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>491-499</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1099/mic.0.2007/013128-0</ELocationID><Abstract><AbstractText>We have previously identified and functionally characterized the transcription factor ACE1 (Pc-ACE1) from Phanerochaete chrysosporium. In Saccharomyces cerevisiae, ACE1 activates the copper-dependent transcription of target genes through a DNA sequence element named ACE. However, the possible target gene(s) of Pc-ACE1 were unknown. An in silico search led to the identification of putative ACE elements in the promoter region of mco1, one of the four clustered genes encoding multicopper oxidases (MCOs) in P. chrysosporium. Since copper exerts an effect at the transcriptional level in MCOs from several organisms, in this work we analysed the effect of copper on transcript levels of the clustered MCO genes from P. chrysosporium, with the exception of the transcriptionally inactive mco3. Copper supplementation of cultures produced an increment in transcripts from genes mco1 and mco2, but not from mco4. Electrophoretic mobility-shift assays revealed that Pc-ACE1 binds specifically to a probe containing one of the putative ACE elements found in the promoter of mco1. In addition, using a cell-free transcription system, we showed that in the presence of cuprous ion, Pc-ACE1 induces activation of the promoter of mco1, but not that of mco2.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Canessa</LastName><ForeName>Paulo</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Álvarez</LastName><ForeName>José Miguel</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Polanco</LastName><ForeName>Rubén</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bull</LastName><ForeName>Paulina</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vicuña</LastName><ForeName>Rafael</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile and Instituto Milenio de Biología Fundamental y Aplicada, Santiago, Chile.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Microbiology (Reading)</MedlineTA><NlmUniqueID>9430468</NlmUniqueID><ISSNLinking>1350-0872</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004268">DNA-Binding Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>789U1901C5</RegistryNumber><NameOfSubstance 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MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D024202" MajorTopicYN="N">Electrophoretic Mobility Shift Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010088" MajorTopicYN="N">Oxidoreductases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="Y">Transcription, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>1</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>4</Month><Day>4</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>1</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18227253</ArticleId><ArticleId IdType="doi">10.1099/mic.0.2007/013128-0</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Chili</li></country></list><tree><country name="Chili"><noRegion><name sortKey="Canessa, Paulo" sort="Canessa, Paulo" uniqKey="Canessa P" first="Paulo" last="Canessa">Paulo Canessa</name></noRegion><name sortKey="Alvarez, Jose Miguel" sort="Alvarez, Jose Miguel" uniqKey="Alvarez J" first="José Miguel" last="Álvarez">José Miguel Álvarez</name><name sortKey="Bull, Paulina" sort="Bull, Paulina" uniqKey="Bull P" first="Paulina" last="Bull">Paulina Bull</name><name sortKey="Polanco, Ruben" sort="Polanco, Ruben" uniqKey="Polanco R" first="Rubén" last="Polanco">Rubén Polanco</name><name sortKey="Vicu A, Rafael" sort="Vicu A, Rafael" uniqKey="Vicu A R" first="Rafael" last="Vicu A">Rafael Vicu A</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:18227253" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhanerochaeteV1
| This area was generated with Dilib version V0.6.37. |  |