Flavin-containing monooxygenases from Phanerochaete chrysosporium responsible for fungal metabolism of phenolic compounds.
Identifieur interne : 000439 ( Main/Exploration ); précédent : 000438; suivant : 000440Flavin-containing monooxygenases from Phanerochaete chrysosporium responsible for fungal metabolism of phenolic compounds.
Auteurs : Tomofumi Nakamura [Japon] ; Hirofumi Ichinose ; Hiroyuki WariishiSource :
- Biodegradation [ 1572-9729 ] ; 2012.
Descripteurs français
- KwdFr :
- Alignement de séquences (MeSH), Benzaldéhydes (métabolisme), Données de séquences moléculaires (MeSH), Dépollution biologique de l'environnement (MeSH), Flavines (métabolisme), Mixed function oxygenases (composition chimique), Mixed function oxygenases (génétique), Mixed function oxygenases (métabolisme), Phanerochaete (composition chimique), Phanerochaete (enzymologie), Phanerochaete (génétique), Phanerochaete (métabolisme), Phénols (métabolisme), Protéines fongiques (composition chimique), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Séquence d'acides aminés (MeSH).
- MESH :
- composition chimique : Mixed function oxygenases, Phanerochaete, Protéines fongiques.
- enzymologie : Phanerochaete.
- génétique : Mixed function oxygenases, Phanerochaete, Protéines fongiques.
- métabolisme : Benzaldéhydes, Flavines, Mixed function oxygenases, Phanerochaete, Phénols, Protéines fongiques.
- Alignement de séquences, Données de séquences moléculaires, Dépollution biologique de l'environnement, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Benzaldehydes (metabolism), Biodegradation, Environmental (MeSH), Flavins (metabolism), Fungal Proteins (chemistry), Fungal Proteins (genetics), Fungal Proteins (metabolism), Mixed Function Oxygenases (chemistry), Mixed Function Oxygenases (genetics), Mixed Function Oxygenases (metabolism), Molecular Sequence Data (MeSH), Phanerochaete (chemistry), Phanerochaete (enzymology), Phanerochaete (genetics), Phanerochaete (metabolism), Phenols (metabolism), Sequence Alignment (MeSH).
- MESH :
- chemical , chemistry : Fungal Proteins, Mixed Function Oxygenases.
- chemical , genetics : Fungal Proteins, Mixed Function Oxygenases.
- chemical , metabolism : Benzaldehydes, Flavins, Fungal Proteins, Mixed Function Oxygenases, Phenols.
- chemistry : Phanerochaete.
- enzymology : Phanerochaete.
- genetics : Phanerochaete.
- metabolism : Phanerochaete.
- Amino Acid Sequence, Biodegradation, Environmental, Molecular Sequence Data, Sequence Alignment.
Abstract
We investigated the cellular responses of the white-rot basidiomycete Phanerochaete chrysosporium against vanillin. Based upon a proteomic survey, it was demonstrated that two flavin-containing monooxygenases (PcFMO1 and PcFMO2) are translationally up-regulated in response to exogenous addition of vanillin. To elucidate their catalytic functions, we cloned cDNAs and heterologously expressed them in Escherichia coli. The recombinant PcFMO1 showed catalytic activities against monocyclic phenols such as phenol, hydroquinone, and 4-chlorophenol. In addition, the product from hydroquinone was identified as 1,2,4-trihydroxybenzene, an important intermediate in a metabolic pathway of aromatic compounds in which the aromatic ring of 1,2,4-trihydroxybenzene can be further cleaved by fungal dioxygenases for mineralization. Thus, the ortho-cleavage pathway of phenolic compounds would presumably be associated with PcFMO1.
DOI: 10.1007/s10532-011-9521-x
PubMed: 22102096
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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last="Ichinose">Hirofumi Ichinose</name></author><author><name sortKey="Wariishi, Hiroyuki" sort="Wariishi, Hiroyuki" uniqKey="Wariishi H" first="Hiroyuki" last="Wariishi">Hiroyuki Wariishi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22102096</idno><idno type="pmid">22102096</idno><idno type="doi">10.1007/s10532-011-9521-x</idno><idno type="wicri:Area/Main/Corpus">000469</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000469</idno><idno type="wicri:Area/Main/Curation">000469</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000469</idno><idno type="wicri:Area/Main/Exploration">000469</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Flavin-containing monooxygenases from Phanerochaete chrysosporium responsible for fungal metabolism of phenolic compounds.</title><author><name sortKey="Nakamura, Tomofumi" sort="Nakamura, Tomofumi" uniqKey="Nakamura T" first="Tomofumi" last="Nakamura">Tomofumi Nakamura</name><affiliation wicri:level="4"><nlm:affiliation>Faculty of Agriculture, Kyushu University, 6-10-1 Hakozi, Higashi-ku, Fukuoka, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Agriculture, Kyushu University, 6-10-1 Hakozi, Higashi-ku, Fukuoka</wicri:regionArea><orgName type="university">Université de Kyūshū</orgName><placeName><settlement type="city">Fukuoka</settlement><region type="province">Kyūshū</region><region type="prefecture">Préfecture de Fukuoka</region></placeName></affiliation></author><author><name sortKey="Ichinose, Hirofumi" sort="Ichinose, Hirofumi" uniqKey="Ichinose H" first="Hirofumi" last="Ichinose">Hirofumi Ichinose</name></author><author><name sortKey="Wariishi, Hiroyuki" sort="Wariishi, Hiroyuki" uniqKey="Wariishi H" first="Hiroyuki" last="Wariishi">Hiroyuki Wariishi</name></author></analytic><series><title level="j">Biodegradation</title><idno type="eISSN">1572-9729</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Benzaldehydes (metabolism)</term><term>Biodegradation, Environmental (MeSH)</term><term>Flavins (metabolism)</term><term>Fungal Proteins (chemistry)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Mixed Function Oxygenases (chemistry)</term><term>Mixed Function Oxygenases (genetics)</term><term>Mixed Function Oxygenases (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Phanerochaete (chemistry)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (genetics)</term><term>Phanerochaete (metabolism)</term><term>Phenols (metabolism)</term><term>Sequence Alignment (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term><term>Benzaldéhydes (métabolisme)</term><term>Données de séquences moléculaires (MeSH)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Flavines (métabolisme)</term><term>Mixed function oxygenases (composition chimique)</term><term>Mixed function oxygenases (génétique)</term><term>Mixed function oxygenases (métabolisme)</term><term>Phanerochaete (composition chimique)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (génétique)</term><term>Phanerochaete (métabolisme)</term><term>Phénols (métabolisme)</term><term>Protéines fongiques (composition chimique)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fungal Proteins</term><term>Mixed Function Oxygenases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>Mixed Function Oxygenases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Benzaldehydes</term><term>Flavins</term><term>Fungal Proteins</term><term>Mixed Function Oxygenases</term><term>Phenols</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Mixed function oxygenases</term><term>Phanerochaete</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Mixed function oxygenases</term><term>Phanerochaete</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Benzaldéhydes</term><term>Flavines</term><term>Mixed function oxygenases</term><term>Phanerochaete</term><term>Phénols</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Biodegradation, Environmental</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Données de séquences moléculaires</term><term>Dépollution biologique de l'environnement</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We investigated the cellular responses of the white-rot basidiomycete Phanerochaete chrysosporium against vanillin. Based upon a proteomic survey, it was demonstrated that two flavin-containing monooxygenases (PcFMO1 and PcFMO2) are translationally up-regulated in response to exogenous addition of vanillin. To elucidate their catalytic functions, we cloned cDNAs and heterologously expressed them in Escherichia coli. The recombinant PcFMO1 showed catalytic activities against monocyclic phenols such as phenol, hydroquinone, and 4-chlorophenol. In addition, the product from hydroquinone was identified as 1,2,4-trihydroxybenzene, an important intermediate in a metabolic pathway of aromatic compounds in which the aromatic ring of 1,2,4-trihydroxybenzene can be further cleaved by fungal dioxygenases for mineralization. Thus, the ortho-cleavage pathway of phenolic compounds would presumably be associated with PcFMO1.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22102096</PMID><DateCompleted><Year>2012</Year><Month>07</Month><Day>30</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1572-9729</ISSN><JournalIssue CitedMedium="Internet"><Volume>23</Volume><Issue>3</Issue><PubDate><Year>2012</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Biodegradation</Title><ISOAbbreviation>Biodegradation</ISOAbbreviation></Journal><ArticleTitle>Flavin-containing monooxygenases from Phanerochaete chrysosporium responsible for fungal metabolism of phenolic compounds.</ArticleTitle><Pagination><MedlinePgn>343-50</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10532-011-9521-x</ELocationID><Abstract><AbstractText>We investigated the cellular responses of the white-rot basidiomycete Phanerochaete chrysosporium against vanillin. Based upon a proteomic survey, it was demonstrated that two flavin-containing monooxygenases (PcFMO1 and PcFMO2) are translationally up-regulated in response to exogenous addition of vanillin. To elucidate their catalytic functions, we cloned cDNAs and heterologously expressed them in Escherichia coli. The recombinant PcFMO1 showed catalytic activities against monocyclic phenols such as phenol, hydroquinone, and 4-chlorophenol. In addition, the product from hydroquinone was identified as 1,2,4-trihydroxybenzene, an important intermediate in a metabolic pathway of aromatic compounds in which the aromatic ring of 1,2,4-trihydroxybenzene can be further cleaved by fungal dioxygenases for mineralization. Thus, the ortho-cleavage pathway of phenolic compounds would presumably be associated with PcFMO1.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nakamura</LastName><ForeName>Tomofumi</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Faculty of Agriculture, Kyushu University, 6-10-1 Hakozi, Higashi-ku, Fukuoka, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ichinose</LastName><ForeName>Hirofumi</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Wariishi</LastName><ForeName>Hiroyuki</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>11</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biodegradation</MedlineTA><NlmUniqueID>9100834</NlmUniqueID><ISSNLinking>0923-9820</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001547">Benzaldehydes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005415">Flavins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010636">Phenols</NameOfSubstance></Chemical><Chemical><RegistryNumber>CHI530446X</RegistryNumber><NameOfSubstance UI="C100058">vanillin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D006899">Mixed Function Oxygenases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001547" MajorTopicYN="N">Benzaldehydes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005415" MajorTopicYN="N">Flavins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006899" MajorTopicYN="N">Mixed Function Oxygenases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010636" MajorTopicYN="N">Phenols</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>12</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>06</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>11</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>7</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22102096</ArticleId><ArticleId IdType="doi">10.1007/s10532-011-9521-x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Kyūshū</li><li>Préfecture de Fukuoka</li></region><settlement><li>Fukuoka</li></settlement><orgName><li>Université de Kyūshū</li></orgName></list><tree><noCountry><name sortKey="Ichinose, Hirofumi" sort="Ichinose, Hirofumi" uniqKey="Ichinose H" first="Hirofumi" last="Ichinose">Hirofumi Ichinose</name><name sortKey="Wariishi, Hiroyuki" sort="Wariishi, Hiroyuki" uniqKey="Wariishi H" first="Hiroyuki" last="Wariishi">Hiroyuki Wariishi</name></noCountry><country name="Japon"><region name="Kyūshū"><name sortKey="Nakamura, Tomofumi" sort="Nakamura, Tomofumi" uniqKey="Nakamura T" first="Tomofumi" last="Nakamura">Tomofumi Nakamura</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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