Biofilm Eradication Using Biogenic Silver Nanoparticles.
Identifieur interne : 000038 ( Main/Exploration ); précédent : 000037; suivant : 000039Biofilm Eradication Using Biogenic Silver Nanoparticles.
Auteurs : María Belén Estevez [Uruguay] ; Sofía Raffaelli [Uruguay] ; Scott G. Mitchell [Espagne] ; Ricardo Faccio [Uruguay] ; Silvana Alborés [Uruguay]Source :
- Molecules (Basel, Switzerland) [ 1420-3049 ] ; 2020.
Abstract
Microorganisms offer an alternative green and scalable technology for the synthesis of value added products. Fungi secrete high quantities of bioactive substances, which play dual-functional roles as both reducing and stabilizing agents in the synthesis of colloidal metal nanoparticles such as silver nanoparticles, which display potent antimicrobial properties that can be harnessed for a number of industrial applications. The aim of this work was the production of silver nanoparticles using the extracellular cell free extracts of Phanerochaete chrysosporium, and to evaluate their activity as antimicrobial and antibiofilm agents. The 45-nm diameter silver nanoparticles synthesized using this methodology possessed a high negative surface charge close to -30 mV and showed colloidal stability from pH 3-9 and under conditions of high ionic strength ([NaCl] = 10-500 mM). A combination of environmental SEM, TEM, and confocal Raman microscopy was used to study the nanoparticle-E. coli interactions to gain a first insight into their antimicrobial mechanisms. Raman data demonstrate a significant decrease in the fatty acid content of E. coli cells, which suggests a loss of the cell membrane integrity after exposure to the PchNPs, which is also commensurate with ESEM and TEM images. Additionally, these biogenic PchNPs displayed biofilm disruption activity for the eradication of E. coli and C. albicans biofilms.
DOI: 10.3390/molecules25092023
PubMed: 32357560
PubMed Central: PMC7249070
Affiliations:
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Mitchell</name><affiliation wicri:level="4"><nlm:affiliation>Instituto de Ciencia de Materiales de Aragón (ICMA), Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Zaragoza, 50009 Zaragoza, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Ciencia de Materiales de Aragón (ICMA), Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Zaragoza, 50009 Zaragoza</wicri:regionArea><placeName><region nuts="2" type="communauté">Aragon</region><settlement type="city">Saragosse</settlement></placeName><orgName type="university">Université de Saragosse</orgName></affiliation><affiliation wicri:level="2"><nlm:affiliation>CIBER-BBN, Instituto de Salud Carlos III, 28029 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>CIBER-BBN, Instituto de Salud Carlos III, 28029 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Faccio, Ricardo" sort="Faccio, Ricardo" uniqKey="Faccio R" first="Ricardo" last="Faccio">Ricardo Faccio</name><affiliation wicri:level="1"><nlm:affiliation>Centro NanoMat & Grupo Física, Departamento de Experimentación y Teoría de la Estructura de la Materia y sus Aplicaciones (DETEMA), Facultad de Química, Universidad de la República, Montevideo 11800, Uruguay.</nlm:affiliation><country xml:lang="fr">Uruguay</country><wicri:regionArea>Centro NanoMat & Grupo Física, Departamento de Experimentación y Teoría de la Estructura de la Materia y sus Aplicaciones (DETEMA), Facultad de Química, Universidad de la República, Montevideo 11800</wicri:regionArea><wicri:noRegion>Montevideo 11800</wicri:noRegion></affiliation></author><author><name sortKey="Albores, Silvana" sort="Albores, Silvana" uniqKey="Albores S" first="Silvana" last="Alborés">Silvana Alborés</name><affiliation wicri:level="1"><nlm:affiliation>Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo 11800, Uruguay.</nlm:affiliation><country xml:lang="fr">Uruguay</country><wicri:regionArea>Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo 11800</wicri:regionArea><wicri:noRegion>Montevideo 11800</wicri:noRegion></affiliation></author></analytic><series><title level="j">Molecules (Basel, Switzerland)</title><idno type="eISSN">1420-3049</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Microorganisms offer an alternative green and scalable technology for the synthesis of value added products. Fungi secrete high quantities of bioactive substances, which play dual-functional roles as both reducing and stabilizing agents in the synthesis of colloidal metal nanoparticles such as silver nanoparticles, which display potent antimicrobial properties that can be harnessed for a number of industrial applications. The aim of this work was the production of silver nanoparticles using the extracellular cell free extracts of <i>Phanerochaete chrysosporium</i>, and to evaluate their activity as antimicrobial and antibiofilm agents. The 45-nm diameter silver nanoparticles synthesized using this methodology possessed a high negative surface charge close to -30 mV and showed colloidal stability from pH 3-9 and under conditions of high ionic strength (<i>[NaCl]</i> = 10-500 mM). A combination of environmental SEM, TEM, and confocal Raman microscopy was used to study the nanoparticle-<i>E. coli</i> interactions to gain a first insight into their antimicrobial mechanisms. Raman data demonstrate a significant decrease in the fatty acid content of <i>E. coli</i> cells, which suggests a loss of the cell membrane integrity after exposure to the PchNPs, which is also commensurate with ESEM and TEM images. Additionally, these biogenic PchNPs displayed biofilm disruption activity for the eradication of <i>E. coli</i> and <i>C. albicans</i> biofilms.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32357560</PMID><DateRevised><Year>2020</Year><Month>06</Month><Day>11</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1420-3049</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>9</Issue><PubDate><Year>2020</Year><Month>Apr</Month><Day>26</Day></PubDate></JournalIssue><Title>Molecules (Basel, Switzerland)</Title><ISOAbbreviation>Molecules</ISOAbbreviation></Journal><ArticleTitle>Biofilm Eradication Using Biogenic Silver Nanoparticles.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E2023</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/molecules25092023</ELocationID><Abstract><AbstractText>Microorganisms offer an alternative green and scalable technology for the synthesis of value added products. Fungi secrete high quantities of bioactive substances, which play dual-functional roles as both reducing and stabilizing agents in the synthesis of colloidal metal nanoparticles such as silver nanoparticles, which display potent antimicrobial properties that can be harnessed for a number of industrial applications. The aim of this work was the production of silver nanoparticles using the extracellular cell free extracts of <i>Phanerochaete chrysosporium</i>, and to evaluate their activity as antimicrobial and antibiofilm agents. The 45-nm diameter silver nanoparticles synthesized using this methodology possessed a high negative surface charge close to -30 mV and showed colloidal stability from pH 3-9 and under conditions of high ionic strength (<i>[NaCl]</i> = 10-500 mM). A combination of environmental SEM, TEM, and confocal Raman microscopy was used to study the nanoparticle-<i>E. coli</i> interactions to gain a first insight into their antimicrobial mechanisms. Raman data demonstrate a significant decrease in the fatty acid content of <i>E. coli</i> cells, which suggests a loss of the cell membrane integrity after exposure to the PchNPs, which is also commensurate with ESEM and TEM images. Additionally, these biogenic PchNPs displayed biofilm disruption activity for the eradication of <i>E. coli</i> and <i>C. albicans</i> biofilms.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Estevez</LastName><ForeName>María Belén</ForeName><Initials>MB</Initials><AffiliationInfo><Affiliation>Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo 11800, Uruguay.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Raffaelli</LastName><ForeName>Sofía</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo 11800, Uruguay.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mitchell</LastName><ForeName>Scott G</ForeName><Initials>SG</Initials><Identifier Source="ORCID">0000-0003-4848-414X</Identifier><AffiliationInfo><Affiliation>Instituto de Ciencia de Materiales de Aragón (ICMA), Consejo Superior de Investigaciones Científicas (CSIC)-Universidad de Zaragoza, 50009 Zaragoza, Spain.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>CIBER-BBN, Instituto de Salud Carlos III, 28029 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Faccio</LastName><ForeName>Ricardo</ForeName><Initials>R</Initials><Identifier Source="ORCID">0000-0003-1650-7677</Identifier><AffiliationInfo><Affiliation>Centro NanoMat & Grupo Física, Departamento de Experimentación y Teoría de la Estructura de la Materia y sus Aplicaciones (DETEMA), Facultad de Química, Universidad de la República, Montevideo 11800, Uruguay.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alborés</LastName><ForeName>Silvana</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0003-3200-3057</Identifier><AffiliationInfo><Affiliation>Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo 11800, Uruguay.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Molecules</MedlineTA><NlmUniqueID>100964009</NlmUniqueID><ISSNLinking>1420-3049</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">antibiofilm</Keyword><Keyword MajorTopicYN="N">antimicrobial</Keyword><Keyword MajorTopicYN="N">biogenic nanoparticles</Keyword><Keyword MajorTopicYN="N">silver nanoparticles</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>03</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>04</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>04</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>5</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>5</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>5</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32357560</ArticleId><ArticleId IdType="pii">molecules25092023</ArticleId><ArticleId IdType="doi">10.3390/molecules25092023</ArticleId><ArticleId IdType="pmc">PMC7249070</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Sci Rep. 2016 Jun 21;6:28443</Citation><ArticleIdList><ArticleId 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21;48(2):428-446</Citation><ArticleIdList><ArticleId IdType="pubmed">30601473</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li><li>Uruguay</li></country><region><li>Aragon</li><li>Communauté de Madrid</li></region><settlement><li>Saragosse</li></settlement><orgName><li>Université de Saragosse</li></orgName></list><tree><country name="Uruguay"><noRegion><name sortKey="Estevez, Maria Belen" sort="Estevez, Maria Belen" uniqKey="Estevez M" first="María Belén" last="Estevez">María Belén Estevez</name></noRegion><name sortKey="Albores, Silvana" sort="Albores, Silvana" uniqKey="Albores S" first="Silvana" last="Alborés">Silvana Alborés</name><name sortKey="Faccio, Ricardo" sort="Faccio, Ricardo" uniqKey="Faccio R" first="Ricardo" last="Faccio">Ricardo Faccio</name><name sortKey="Raffaelli, Sofia" sort="Raffaelli, Sofia" uniqKey="Raffaelli S" first="Sofía" last="Raffaelli">Sofía Raffaelli</name></country><country name="Espagne"><region name="Aragon"><name sortKey="Mitchell, Scott G" sort="Mitchell, Scott G" uniqKey="Mitchell S" first="Scott G" last="Mitchell">Scott G. 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