Oxidized glutathione promotes association between Eukaryotic Translation Elongation Factor 1Bγ and Ure2p glutathione transferase from Phanerochaete chrysosporium.
Identifieur interne : 000016 ( Main/Exploration ); précédent : 000015; suivant : 000017Oxidized glutathione promotes association between Eukaryotic Translation Elongation Factor 1Bγ and Ure2p glutathione transferase from Phanerochaete chrysosporium.
Auteurs : Raphael Bchini [France] ; Jean-Michel Girardet [France] ; Rodnay Sormani [France] ; Eric Gelhaye [France] ; Mélanie Morel-Rouhier [France]Source :
- The FEBS journal [ 1742-4658 ] ; 2020.
Abstract
The Eukaryotic Translation Elongation Factor 1Bγ (eEF1Bγ) is an atypical member of the glutathione transferase (GST) superfamily. Contrary to more classical GSTs having a role in toxic compound detoxification, eEF1Bγ is suggested to act as a scaffold protein, anchoring the elongation factor complex EF1B to the endoplasmic reticulum. In this study we show that eEF1Bγ from the basidiomycete Phanerochaete chrysosporium is fully active as a glutathione transferase in vitro and undergoes conformational changes upon binding of oxidized glutathione. Using real-time analyses of biomolecular interactions, we show that GSSG allows eEF1Bγ to physically interact with other GSTs from the Ure2p class, opening new perspectives for a better understanding of the role of eEF1Bγ in cellular oxidative stress response.
DOI: 10.1111/febs.15614
PubMed: 33124131
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Oxidized glutathione promotes association between Eukaryotic Translation Elongation Factor 1Bγ and Ure2p glutathione transferase from Phanerochaete chrysosporium.</title>
<author><name sortKey="Bchini, Raphael" sort="Bchini, Raphael" uniqKey="Bchini R" first="Raphael" last="Bchini">Raphael Bchini</name>
<affiliation wicri:level="3"><nlm:affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Girardet, Jean Michel" sort="Girardet, Jean Michel" uniqKey="Girardet J" first="Jean-Michel" last="Girardet">Jean-Michel Girardet</name>
<affiliation wicri:level="3"><nlm:affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sormani, Rodnay" sort="Sormani, Rodnay" uniqKey="Sormani R" first="Rodnay" last="Sormani">Rodnay Sormani</name>
<affiliation wicri:level="3"><nlm:affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gelhaye, Eric" sort="Gelhaye, Eric" uniqKey="Gelhaye E" first="Eric" last="Gelhaye">Eric Gelhaye</name>
<affiliation wicri:level="3"><nlm:affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Morel Rouhier, Melanie" sort="Morel Rouhier, Melanie" uniqKey="Morel Rouhier M" first="Mélanie" last="Morel-Rouhier">Mélanie Morel-Rouhier</name>
<affiliation wicri:level="3"><nlm:affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2020">2020</date>
<idno type="RBID">pubmed:33124131</idno>
<idno type="pmid">33124131</idno>
<idno type="doi">10.1111/febs.15614</idno>
<idno type="wicri:Area/Main/Corpus">000002</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000002</idno>
<idno type="wicri:Area/Main/Curation">000002</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000002</idno>
<idno type="wicri:Area/Main/Exploration">000002</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Oxidized glutathione promotes association between Eukaryotic Translation Elongation Factor 1Bγ and Ure2p glutathione transferase from Phanerochaete chrysosporium.</title>
<author><name sortKey="Bchini, Raphael" sort="Bchini, Raphael" uniqKey="Bchini R" first="Raphael" last="Bchini">Raphael Bchini</name>
<affiliation wicri:level="3"><nlm:affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Girardet, Jean Michel" sort="Girardet, Jean Michel" uniqKey="Girardet J" first="Jean-Michel" last="Girardet">Jean-Michel Girardet</name>
<affiliation wicri:level="3"><nlm:affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sormani, Rodnay" sort="Sormani, Rodnay" uniqKey="Sormani R" first="Rodnay" last="Sormani">Rodnay Sormani</name>
<affiliation wicri:level="3"><nlm:affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gelhaye, Eric" sort="Gelhaye, Eric" uniqKey="Gelhaye E" first="Eric" last="Gelhaye">Eric Gelhaye</name>
<affiliation wicri:level="3"><nlm:affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Morel Rouhier, Melanie" sort="Morel Rouhier, Melanie" uniqKey="Morel Rouhier M" first="Mélanie" last="Morel-Rouhier">Mélanie Morel-Rouhier</name>
<affiliation wicri:level="3"><nlm:affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy</wicri:regionArea>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">The FEBS journal</title>
<idno type="eISSN">1742-4658</idno>
<imprint><date when="2020" type="published">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The Eukaryotic Translation Elongation Factor 1Bγ (eEF1Bγ) is an atypical member of the glutathione transferase (GST) superfamily. Contrary to more classical GSTs having a role in toxic compound detoxification, eEF1Bγ is suggested to act as a scaffold protein, anchoring the elongation factor complex EF1B to the endoplasmic reticulum. In this study we show that eEF1Bγ from the basidiomycete Phanerochaete chrysosporium is fully active as a glutathione transferase in vitro and undergoes conformational changes upon binding of oxidized glutathione. Using real-time analyses of biomolecular interactions, we show that GSSG allows eEF1Bγ to physically interact with other GSTs from the Ure2p class, opening new perspectives for a better understanding of the role of eEF1Bγ in cellular oxidative stress response.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33124131</PMID>
<DateRevised><Year>2020</Year>
<Month>10</Month>
<Day>30</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1742-4658</ISSN>
<JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year>
<Month>Oct</Month>
<Day>30</Day>
</PubDate>
</JournalIssue>
<Title>The FEBS journal</Title>
<ISOAbbreviation>FEBS J</ISOAbbreviation>
</Journal>
<ArticleTitle>Oxidized glutathione promotes association between Eukaryotic Translation Elongation Factor 1Bγ and Ure2p glutathione transferase from Phanerochaete chrysosporium.</ArticleTitle>
<ELocationID EIdType="doi" ValidYN="Y">10.1111/febs.15614</ELocationID>
<Abstract><AbstractText>The Eukaryotic Translation Elongation Factor 1Bγ (eEF1Bγ) is an atypical member of the glutathione transferase (GST) superfamily. Contrary to more classical GSTs having a role in toxic compound detoxification, eEF1Bγ is suggested to act as a scaffold protein, anchoring the elongation factor complex EF1B to the endoplasmic reticulum. In this study we show that eEF1Bγ from the basidiomycete Phanerochaete chrysosporium is fully active as a glutathione transferase in vitro and undergoes conformational changes upon binding of oxidized glutathione. Using real-time analyses of biomolecular interactions, we show that GSSG allows eEF1Bγ to physically interact with other GSTs from the Ure2p class, opening new perspectives for a better understanding of the role of eEF1Bγ in cellular oxidative stress response.</AbstractText>
<CopyrightInformation>This article is protected by copyright. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bchini</LastName>
<ForeName>Raphael</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Girardet</LastName>
<ForeName>Jean-Michel</ForeName>
<Initials>JM</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sormani</LastName>
<ForeName>Rodnay</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gelhaye</LastName>
<ForeName>Eric</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Morel-Rouhier</LastName>
<ForeName>Mélanie</ForeName>
<Initials>M</Initials>
<Identifier Source="ORCID">https://orcid.org/0000-0001-5149-0474</Identifier>
<AffiliationInfo><Affiliation>Université de Lorraine INRAE, Interactions Arbres/Micro-organismes (IAM), UMR 1136, F-54000, Nancy, France.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>10</Month>
<Day>30</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>FEBS J</MedlineTA>
<NlmUniqueID>101229646</NlmUniqueID>
<ISSNLinking>1742-464X</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">GSSG</Keyword>
<Keyword MajorTopicYN="N">eEF1Bγ</Keyword>
<Keyword MajorTopicYN="N">glutathione transferase</Keyword>
<Keyword MajorTopicYN="N">time-resolved molecular dynamics</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year>
<Month>06</Month>
<Day>05</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2020</Year>
<Month>09</Month>
<Day>10</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2020</Year>
<Month>10</Month>
<Day>19</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2020</Year>
<Month>10</Month>
<Day>30</Day>
<Hour>6</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2020</Year>
<Month>10</Month>
<Day>31</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>10</Month>
<Day>31</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>aheadofprint</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">33124131</ArticleId>
<ArticleId IdType="doi">10.1111/febs.15614</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>France</li>
</country>
<region><li>Grand Est</li>
<li>Lorraine (région)</li>
</region>
<settlement><li>Nancy</li>
</settlement>
</list>
<tree><country name="France"><region name="Grand Est"><name sortKey="Bchini, Raphael" sort="Bchini, Raphael" uniqKey="Bchini R" first="Raphael" last="Bchini">Raphael Bchini</name>
</region>
<name sortKey="Gelhaye, Eric" sort="Gelhaye, Eric" uniqKey="Gelhaye E" first="Eric" last="Gelhaye">Eric Gelhaye</name>
<name sortKey="Girardet, Jean Michel" sort="Girardet, Jean Michel" uniqKey="Girardet J" first="Jean-Michel" last="Girardet">Jean-Michel Girardet</name>
<name sortKey="Morel Rouhier, Melanie" sort="Morel Rouhier, Melanie" uniqKey="Morel Rouhier M" first="Mélanie" last="Morel-Rouhier">Mélanie Morel-Rouhier</name>
<name sortKey="Sormani, Rodnay" sort="Sormani, Rodnay" uniqKey="Sormani R" first="Rodnay" last="Sormani">Rodnay Sormani</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000016 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000016 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= PhanerochaeteV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:33124131 |texte= Oxidized glutathione promotes association between Eukaryotic Translation Elongation Factor 1Bγ and Ure2p glutathione transferase from Phanerochaete chrysosporium. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:33124131" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a PhanerochaeteV1
This area was generated with Dilib version V0.6.37. |