Genomic organization of lignin peroxidase genes of Phanerochaete chrysosporium.
Identifieur interne : 000F25 ( Main/Curation ); précédent : 000F24; suivant : 000F26Genomic organization of lignin peroxidase genes of Phanerochaete chrysosporium.
Auteurs : J. Gaskell [États-Unis] ; E. Dieperink ; D. CullenSource :
- Nucleic acids research [ 0305-1048 ] ; 1991.
Descripteurs français
- KwdFr :
- ADN fongique (génétique), Basidiomycota (génétique), Cartographie chromosomique (MeSH), Cartographie de restriction (MeSH), Clonage moléculaire (MeSH), Cosmides (MeSH), Données de séquences moléculaires (MeSH), Gènes fongiques (MeSH), Peroxidases (génétique), Sondes oligonucléotidiques (composition chimique), Séquence nucléotidique (MeSH), Technique de Southern (MeSH).
- MESH :
English descriptors
- KwdEn :
- Base Sequence (MeSH), Basidiomycota (genetics), Blotting, Southern (MeSH), Chromosome Mapping (MeSH), Cloning, Molecular (MeSH), Cosmids (MeSH), DNA, Fungal (genetics), Genes, Fungal (MeSH), Molecular Sequence Data (MeSH), Oligonucleotide Probes (chemistry), Peroxidases (genetics), Restriction Mapping (MeSH).
- MESH :
- chemical , chemistry : Oligonucleotide Probes.
- chemical , genetics : DNA, Fungal, Peroxidases.
- genetics : Basidiomycota.
- Base Sequence, Blotting, Southern, Chromosome Mapping, Cloning, Molecular, Cosmids, Genes, Fungal, Molecular Sequence Data, Restriction Mapping.
Abstract
Three lignin peroxidase (LiP) genes from the basidiomycete Phanerochaete chrysosporium were cloned on a single 30 kb cosmid insert. One gene, GLG5, is the genomic equivalent of a previously reported cDNA clone, CLG5. The other two LiP genes are transcriptionally convergent and map to a position approximately 15 kb downstream of GLG5. The translational stop codons of these genes are separated by 1.3 kb. Analysis of homokaryons established allelic relationships to previously described LiP clones. Using clamped homogeneous electrical field electrophoresis (CHEF), seven chromosomal bands were resolved from P. chrysosporium genomic DNA. On CHEF gel Southern blots, the LiP gene family was localized to a single, dimorphic chromosome.
DOI: 10.1093/nar/19.3.599
PubMed: 2011531
PubMed Central: PMC333654
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<term>Basidiomycota (genetics)</term>
<term>Blotting, Southern (MeSH)</term>
<term>Chromosome Mapping (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Cosmids (MeSH)</term>
<term>DNA, Fungal (genetics)</term>
<term>Genes, Fungal (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Oligonucleotide Probes (chemistry)</term>
<term>Peroxidases (genetics)</term>
<term>Restriction Mapping (MeSH)</term>
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<term>Basidiomycota (génétique)</term>
<term>Cartographie chromosomique (MeSH)</term>
<term>Cartographie de restriction (MeSH)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Cosmides (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Gènes fongiques (MeSH)</term>
<term>Peroxidases (génétique)</term>
<term>Sondes oligonucléotidiques (composition chimique)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Technique de Southern (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Oligonucleotide Probes</term>
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<term>Peroxidases</term>
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<term>Basidiomycota</term>
<term>Peroxidases</term>
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<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Blotting, Southern</term>
<term>Chromosome Mapping</term>
<term>Cloning, Molecular</term>
<term>Cosmids</term>
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<term>Molecular Sequence Data</term>
<term>Restriction Mapping</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Cartographie chromosomique</term>
<term>Cartographie de restriction</term>
<term>Clonage moléculaire</term>
<term>Cosmides</term>
<term>Données de séquences moléculaires</term>
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<front><div type="abstract" xml:lang="en">Three lignin peroxidase (LiP) genes from the basidiomycete Phanerochaete chrysosporium were cloned on a single 30 kb cosmid insert. One gene, GLG5, is the genomic equivalent of a previously reported cDNA clone, CLG5. The other two LiP genes are transcriptionally convergent and map to a position approximately 15 kb downstream of GLG5. The translational stop codons of these genes are separated by 1.3 kb. Analysis of homokaryons established allelic relationships to previously described LiP clones. Using clamped homogeneous electrical field electrophoresis (CHEF), seven chromosomal bands were resolved from P. chrysosporium genomic DNA. On CHEF gel Southern blots, the LiP gene family was localized to a single, dimorphic chromosome.</div>
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<Abstract><AbstractText>Three lignin peroxidase (LiP) genes from the basidiomycete Phanerochaete chrysosporium were cloned on a single 30 kb cosmid insert. One gene, GLG5, is the genomic equivalent of a previously reported cDNA clone, CLG5. The other two LiP genes are transcriptionally convergent and map to a position approximately 15 kb downstream of GLG5. The translational stop codons of these genes are separated by 1.3 kb. Analysis of homokaryons established allelic relationships to previously described LiP clones. Using clamped homogeneous electrical field electrophoresis (CHEF), seven chromosomal bands were resolved from P. chrysosporium genomic DNA. On CHEF gel Southern blots, the LiP gene family was localized to a single, dimorphic chromosome.</AbstractText>
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