Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium.
Identifieur interne : 000E31 ( Main/Curation ); précédent : 000E30; suivant : 000E32Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium.
Auteurs : L. Akileswaran ; M. Alic ; E K Clark ; J L Hornick ; M H GoldSource :
- Current genetics [ 0172-8083 ] ; 1993.
Descripteurs français
- KwdFr :
- Acide orotique (analogues et dérivés), Acide orotique (pharmacologie), Ascomycota (génétique), Basidiomycota (génétique), Basidiomycota (métabolisme), Gènes fongiques (MeSH), Lignine (métabolisme), Mutation (MeSH), Orotate phosphoribosyltransferase (génétique), Orotine 5'-phosphate decarboxylase (génétique), Recombinaison génétique (MeSH), Résistance microbienne aux médicaments (génétique), Transformation génétique (MeSH), Uracile (métabolisme).
- MESH :
- analogues et dérivés : Acide orotique.
- génétique : Ascomycota, Basidiomycota, Orotate phosphoribosyltransferase, Orotine 5'-phosphate decarboxylase, Résistance microbienne aux médicaments.
- métabolisme : Basidiomycota, Lignine, Uracile.
- pharmacologie : Acide orotique.
- Gènes fongiques, Mutation, Recombinaison génétique, Transformation génétique.
English descriptors
- KwdEn :
- Ascomycota (genetics), Basidiomycota (genetics), Basidiomycota (metabolism), Drug Resistance, Microbial (genetics), Genes, Fungal (MeSH), Lignin (metabolism), Mutation (MeSH), Orotate Phosphoribosyltransferase (genetics), Orotic Acid (analogs & derivatives), Orotic Acid (pharmacology), Orotidine-5'-Phosphate Decarboxylase (genetics), Recombination, Genetic (MeSH), Transformation, Genetic (MeSH), Uracil (metabolism).
- MESH :
- chemical , analogs & derivatives : Orotic Acid.
- chemical , genetics : Orotate Phosphoribosyltransferase, Orotidine-5'-Phosphate Decarboxylase.
- chemical , metabolism : Lignin, Uracil.
- genetics : Ascomycota, Basidiomycota, Drug Resistance, Microbial.
- metabolism : Basidiomycota.
- chemical , pharmacology : Orotic Acid.
- Genes, Fungal, Mutation, Recombination, Genetic, Transformation, Genetic.
Abstract
Uracil auxotrophs of Phanerochaete chrysosporium were isolated using 5-fluoroorotate resistance as a selection scheme. The ura3 auxotrophs deficient in orotidylate decarboxylase and ura5 auxotrophs deficient in orotate phosphoribosyl transferase were characterized by enzyme assays and complementation tests. The ura5 auxotrophs were transformed to prototrophy with the ura5 gene from the ascomycete Podospora anserina. The ura3 auxotrophs were transformed to prototrophy with the ura3 gene from the basidiomycete Schizophyllum commune. The P. chrysosporium ura3 gene was isolated from a lambda EMBL3 genomic library using the S. commune ura3 gene as a probe. A 6.6-kb fragment incorporating the ura3 gene was subcloned into Bluescript SK+(pURA3.1) and used to transform P. chrysosporium ura3 auxotrophic strains. The pURA3.1 insert was mapped for restriction sites and the approximate location of the ura3 gene within the insert was determined. Double auxotrophic strains were transformed with either of two marker genes and the resulting single auxotrophic strains were crossed to demonstrate genetic recombination between two nuclei of identical genetic background.
DOI: 10.1007/BF00310898
PubMed: 8467534
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L. Akileswaran<affiliation><nlm:affiliation>Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, Beaverton 97006-1999.</nlm:affiliation><wicri:noCountry code="subField">Beaverton 97006-1999</wicri:noCountry></affiliation>Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium.</title><author><name sortKey="Akileswaran, L" sort="Akileswaran, L" uniqKey="Akileswaran L" first="L" last="Akileswaran">L. Akileswaran</name><affiliation><nlm:affiliation>Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, Beaverton 97006-1999.</nlm:affiliation><wicri:noCountry code="subField">Beaverton 97006-1999</wicri:noCountry></affiliation></author><author><name sortKey="Alic, M" sort="Alic, M" uniqKey="Alic M" first="M" last="Alic">M. Alic</name></author><author><name sortKey="Clark, E K" sort="Clark, E K" uniqKey="Clark E" first="E K" last="Clark">E K Clark</name></author><author><name sortKey="Hornick, J L" sort="Hornick, J L" uniqKey="Hornick J" first="J L" last="Hornick">J L Hornick</name></author><author><name sortKey="Gold, M H" sort="Gold, M H" uniqKey="Gold M" first="M H" last="Gold">M H Gold</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1993">1993</date><idno type="RBID">pubmed:8467534</idno><idno type="pmid">8467534</idno><idno type="doi">10.1007/BF00310898</idno><idno type="wicri:Area/Main/Corpus">000E31</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000E31</idno><idno type="wicri:Area/Main/Curation">000E31</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000E31</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium.</title><author><name sortKey="Akileswaran, L" sort="Akileswaran, L" uniqKey="Akileswaran L" first="L" last="Akileswaran">L. Akileswaran</name><affiliation><nlm:affiliation>Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, Beaverton 97006-1999.</nlm:affiliation><wicri:noCountry code="subField">Beaverton 97006-1999</wicri:noCountry></affiliation></author><author><name sortKey="Alic, M" sort="Alic, M" uniqKey="Alic M" first="M" last="Alic">M. Alic</name></author><author><name sortKey="Clark, E K" sort="Clark, E K" uniqKey="Clark E" first="E K" last="Clark">E K Clark</name></author><author><name sortKey="Hornick, J L" sort="Hornick, J L" uniqKey="Hornick J" first="J L" last="Hornick">J L Hornick</name></author><author><name sortKey="Gold, M H" sort="Gold, M H" uniqKey="Gold M" first="M H" last="Gold">M H Gold</name></author></analytic><series><title level="j">Current genetics</title><idno type="ISSN">0172-8083</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (genetics)</term><term>Basidiomycota (genetics)</term><term>Basidiomycota (metabolism)</term><term>Drug Resistance, Microbial (genetics)</term><term>Genes, Fungal (MeSH)</term><term>Lignin (metabolism)</term><term>Mutation (MeSH)</term><term>Orotate Phosphoribosyltransferase (genetics)</term><term>Orotic Acid (analogs & derivatives)</term><term>Orotic Acid (pharmacology)</term><term>Orotidine-5'-Phosphate Decarboxylase (genetics)</term><term>Recombination, Genetic (MeSH)</term><term>Transformation, Genetic (MeSH)</term><term>Uracil (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide orotique (analogues et dérivés)</term><term>Acide orotique (pharmacologie)</term><term>Ascomycota (génétique)</term><term>Basidiomycota (génétique)</term><term>Basidiomycota (métabolisme)</term><term>Gènes fongiques (MeSH)</term><term>Lignine (métabolisme)</term><term>Mutation (MeSH)</term><term>Orotate phosphoribosyltransferase (génétique)</term><term>Orotine 5'-phosphate decarboxylase (génétique)</term><term>Recombinaison génétique (MeSH)</term><term>Résistance microbienne aux médicaments (génétique)</term><term>Transformation génétique (MeSH)</term><term>Uracile (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Orotic Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Orotate Phosphoribosyltransferase</term><term>Orotidine-5'-Phosphate Decarboxylase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term><term>Uracil</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Acide orotique</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ascomycota</term><term>Basidiomycota</term><term>Drug Resistance, Microbial</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Ascomycota</term><term>Basidiomycota</term><term>Orotate phosphoribosyltransferase</term><term>Orotine 5'-phosphate decarboxylase</term><term>Résistance microbienne aux médicaments</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Basidiomycota</term><term>Lignine</term><term>Uracile</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acide orotique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Orotic Acid</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genes, Fungal</term><term>Mutation</term><term>Recombination, Genetic</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Gènes fongiques</term><term>Mutation</term><term>Recombinaison génétique</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Uracil auxotrophs of Phanerochaete chrysosporium were isolated using 5-fluoroorotate resistance as a selection scheme. The ura3 auxotrophs deficient in orotidylate decarboxylase and ura5 auxotrophs deficient in orotate phosphoribosyl transferase were characterized by enzyme assays and complementation tests. The ura5 auxotrophs were transformed to prototrophy with the ura5 gene from the ascomycete Podospora anserina. The ura3 auxotrophs were transformed to prototrophy with the ura3 gene from the basidiomycete Schizophyllum commune. The P. chrysosporium ura3 gene was isolated from a lambda EMBL3 genomic library using the S. commune ura3 gene as a probe. A 6.6-kb fragment incorporating the ura3 gene was subcloned into Bluescript SK+(pURA3.1) and used to transform P. chrysosporium ura3 auxotrophic strains. The pURA3.1 insert was mapped for restriction sites and the approximate location of the ura3 gene within the insert was determined. Double auxotrophic strains were transformed with either of two marker genes and the resulting single auxotrophic strains were crossed to demonstrate genetic recombination between two nuclei of identical genetic background.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8467534</PMID><DateCompleted><Year>1993</Year><Month>05</Month><Day>12</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0172-8083</ISSN><JournalIssue CitedMedium="Print"><Volume>23</Volume><Issue>4</Issue><PubDate><Year>1993</Year></PubDate></JournalIssue><Title>Current genetics</Title><ISOAbbreviation>Curr Genet</ISOAbbreviation></Journal><ArticleTitle>Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>351-6</MedlinePgn></Pagination><Abstract><AbstractText>Uracil auxotrophs of Phanerochaete chrysosporium were isolated using 5-fluoroorotate resistance as a selection scheme. The ura3 auxotrophs deficient in orotidylate decarboxylase and ura5 auxotrophs deficient in orotate phosphoribosyl transferase were characterized by enzyme assays and complementation tests. The ura5 auxotrophs were transformed to prototrophy with the ura5 gene from the ascomycete Podospora anserina. The ura3 auxotrophs were transformed to prototrophy with the ura3 gene from the basidiomycete Schizophyllum commune. The P. chrysosporium ura3 gene was isolated from a lambda EMBL3 genomic library using the S. commune ura3 gene as a probe. A 6.6-kb fragment incorporating the ura3 gene was subcloned into Bluescript SK+(pURA3.1) and used to transform P. chrysosporium ura3 auxotrophic strains. The pURA3.1 insert was mapped for restriction sites and the approximate location of the ura3 gene within the insert was determined. Double auxotrophic strains were transformed with either of two marker genes and the resulting single auxotrophic strains were crossed to demonstrate genetic recombination between two nuclei of identical genetic background.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Akileswaran</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, Beaverton 97006-1999.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alic</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Clark</LastName><ForeName>E K</ForeName><Initials>EK</Initials></Author><Author ValidYN="Y"><LastName>Hornick</LastName><ForeName>J L</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Gold</LastName><ForeName>M H</ForeName><Initials>MH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Curr Genet</MedlineTA><NlmUniqueID>8004904</NlmUniqueID><ISSNLinking>0172-8083</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>56HH86ZVCT</RegistryNumber><NameOfSubstance UI="D014498">Uracil</NameOfSubstance></Chemical><Chemical><RegistryNumber>61H4T033E5</RegistryNumber><NameOfSubstance UI="D009963">Orotic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>7IA9OUC93E</RegistryNumber><NameOfSubstance UI="C001242">5-fluoroorotic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.4.2.10</RegistryNumber><NameOfSubstance UI="D009962">Orotate Phosphoribosyltransferase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.1.1.23</RegistryNumber><NameOfSubstance UI="D009964">Orotidine-5'-Phosphate Decarboxylase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><GeneSymbolList><GeneSymbol>ura3</GeneSymbol><GeneSymbol>ura5</GeneSymbol></GeneSymbolList><MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004352" MajorTopicYN="N">Drug Resistance, Microbial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009962" MajorTopicYN="N">Orotate Phosphoribosyltransferase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009963" MajorTopicYN="N">Orotic Acid</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009964" MajorTopicYN="N">Orotidine-5'-Phosphate Decarboxylase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011995" MajorTopicYN="N">Recombination, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014498" MajorTopicYN="N">Uracil</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1993</Year><Month>1</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1993</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate 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