Gene replacement in the lignin-degrading basidiomycete Phanerochaete chrysosporium.
Identifieur interne : 000D74 ( Main/Curation ); précédent : 000D73; suivant : 000D75Gene replacement in the lignin-degrading basidiomycete Phanerochaete chrysosporium.
Auteurs : M. Alic ; L. Akileswaran ; M H GoldSource :
- Gene [ 0378-1119 ] ; 1993.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Basidiomycota, Orotine 5'-phosphate decarboxylase.
- métabolisme : Basidiomycota, Lignine.
- Technique de Southern, Transformation génétique.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Orotidine-5'-Phosphate Decarboxylase.
- chemical , metabolism : Lignin.
- genetics : Basidiomycota.
- metabolism : Basidiomycota.
- Blotting, Southern, Transformation, Genetic.
Abstract
The ability to carry out gene replacements and gene targeting in the lignin-degrading basidiomycete fungus, Phanerochaete chrysosporium, would facilitate studies on the roles and regulation of various components of its lignindegrading system. A plasmid consisting of the P. chrysosporium ura3 gene (encoding orotidylate decarboxylase) interrupted with the Schizophyllum commune ade2 gene (encoding an adenine biosynthetic enzyme) was used to transform the P. chrysosporium ade2 strain to adenine prototrophy with selection on 5-fluoroorotic acid for inactivation of the ura3 gene. Stable Ade+Ura- strains were obtained at a frequency of approximately one transformant per microgram of DNA. In all of the Ade+Ura- transformants examined by Southern analysis, the chromosomal ura3 locus had been replaced by the plasmid insert.
DOI: 10.1016/0378-1119(93)90485-l
PubMed: 8294022
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M. Alic<affiliation><nlm:affiliation>Department of Chemistry, Biochemistry, and Molecular Biology, Oregon Graduate Institute of Science and Technology, Beaverton 97006.</nlm:affiliation>
<wicri:noCountry code="subField">Beaverton 97006</wicri:noCountry>
</affiliation>
Le document en format XML
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<author><name sortKey="Akileswaran, L" sort="Akileswaran, L" uniqKey="Akileswaran L" first="L" last="Akileswaran">L. Akileswaran</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term>
<term>Basidiomycota (metabolism)</term>
<term>Blotting, Southern (MeSH)</term>
<term>Lignin (metabolism)</term>
<term>Orotidine-5'-Phosphate Decarboxylase (genetics)</term>
<term>Transformation, Genetic (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (génétique)</term>
<term>Basidiomycota (métabolisme)</term>
<term>Lignine (métabolisme)</term>
<term>Orotine 5'-phosphate decarboxylase (génétique)</term>
<term>Technique de Southern (MeSH)</term>
<term>Transformation génétique (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Orotidine-5'-Phosphate Decarboxylase</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term>
<term>Orotine 5'-phosphate decarboxylase</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Basidiomycota</term>
<term>Lignine</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Blotting, Southern</term>
<term>Transformation, Genetic</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Technique de Southern</term>
<term>Transformation génétique</term>
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<front><div type="abstract" xml:lang="en">The ability to carry out gene replacements and gene targeting in the lignin-degrading basidiomycete fungus, Phanerochaete chrysosporium, would facilitate studies on the roles and regulation of various components of its lignindegrading system. A plasmid consisting of the P. chrysosporium ura3 gene (encoding orotidylate decarboxylase) interrupted with the Schizophyllum commune ade2 gene (encoding an adenine biosynthetic enzyme) was used to transform the P. chrysosporium ade2 strain to adenine prototrophy with selection on 5-fluoroorotic acid for inactivation of the ura3 gene. Stable Ade+Ura- strains were obtained at a frequency of approximately one transformant per microgram of DNA. In all of the Ade+Ura- transformants examined by Southern analysis, the chromosomal ura3 locus had been replaced by the plasmid insert.</div>
</front>
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<Month>03</Month>
<Day>01</Day>
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<DateRevised><Year>2019</Year>
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<Issue>1-2</Issue>
<PubDate><Year>1993</Year>
<Month>Dec</Month>
<Day>22</Day>
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<Title>Gene</Title>
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<ArticleTitle>Gene replacement in the lignin-degrading basidiomycete Phanerochaete chrysosporium.</ArticleTitle>
<Pagination><MedlinePgn>307-11</MedlinePgn>
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<Abstract><AbstractText>The ability to carry out gene replacements and gene targeting in the lignin-degrading basidiomycete fungus, Phanerochaete chrysosporium, would facilitate studies on the roles and regulation of various components of its lignindegrading system. A plasmid consisting of the P. chrysosporium ura3 gene (encoding orotidylate decarboxylase) interrupted with the Schizophyllum commune ade2 gene (encoding an adenine biosynthetic enzyme) was used to transform the P. chrysosporium ade2 strain to adenine prototrophy with selection on 5-fluoroorotic acid for inactivation of the ura3 gene. Stable Ade+Ura- strains were obtained at a frequency of approximately one transformant per microgram of DNA. In all of the Ade+Ura- transformants examined by Southern analysis, the chromosomal ura3 locus had been replaced by the plasmid insert.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Alic</LastName>
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<AffiliationInfo><Affiliation>Department of Chemistry, Biochemistry, and Molecular Biology, Oregon Graduate Institute of Science and Technology, Beaverton 97006.</Affiliation>
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<Language>eng</Language>
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<ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber>
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<Chemical><RegistryNumber>EC 4.1.1.23</RegistryNumber>
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<GeneSymbolList><GeneSymbol>ade2</GeneSymbol>
<GeneSymbol>ura3</GeneSymbol>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName>
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<MeshHeading><DescriptorName UI="D015139" MajorTopicYN="N">Blotting, Southern</DescriptorName>
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