A PCR method for the detection and differentiation of Lentinus edodes and Trametes versicolor in defined-mixed cultures used for wastewater treatment.
Identifieur interne : 000869 ( Main/Curation ); précédent : 000868; suivant : 000870A PCR method for the detection and differentiation of Lentinus edodes and Trametes versicolor in defined-mixed cultures used for wastewater treatment.
Auteurs : Jaime García-Mena [Mexique] ; Claudia Cano-Ramirez ; Claudio Garibay-Orijel ; Sergio Ramirez-Canseco ; Héctor M. Poggi-VaraldoSource :
- Applied microbiology and biotechnology [ 0175-7598 ] ; 2005.
Descripteurs français
- KwdFr :
- ADN fongique (analyse), Analyse de séquence d'ADN (MeSH), Champignons shiitake (classification), Champignons shiitake (croissance et développement), Champignons shiitake (génétique), Champignons shiitake (isolement et purification), Données de séquences moléculaires (MeSH), Laccase (génétique), Microbiologie de l'eau (MeSH), Milieux de culture (MeSH), Phanerochaete (classification), Phanerochaete (croissance et développement), Phanerochaete (génétique), Phanerochaete (isolement et purification), Polyporales (classification), Polyporales (croissance et développement), Polyporales (génétique), Polyporales (isolement et purification), Réaction de polymérisation en chaîne (méthodes), Techniques de typage mycologique (MeSH), Élimination des déchets liquides (méthodes).
- MESH :
- analyse : ADN fongique.
- croissance et développement : Champignons shiitake, Phanerochaete, Polyporales.
- génétique : Champignons shiitake, Laccase, Phanerochaete, Polyporales.
- isolement et purification : Champignons shiitake, Phanerochaete, Polyporales.
- méthodes : Réaction de polymérisation en chaîne, Élimination des déchets liquides.
- Analyse de séquence d'ADN, Données de séquences moléculaires, Microbiologie de l'eau, Milieux de culture, Techniques de typage mycologique.
English descriptors
- KwdEn :
- Culture Media (MeSH), DNA, Fungal (analysis), Laccase (genetics), Molecular Sequence Data (MeSH), Mycological Typing Techniques (MeSH), Phanerochaete (classification), Phanerochaete (genetics), Phanerochaete (growth & development), Phanerochaete (isolation & purification), Polymerase Chain Reaction (methods), Polyporales (classification), Polyporales (genetics), Polyporales (growth & development), Polyporales (isolation & purification), Sequence Analysis, DNA (MeSH), Shiitake Mushrooms (classification), Shiitake Mushrooms (genetics), Shiitake Mushrooms (growth & development), Shiitake Mushrooms (isolation & purification), Waste Disposal, Fluid (methods), Water Microbiology (MeSH).
- MESH :
- chemical , analysis : DNA, Fungal.
- chemical , genetics : Laccase.
- chemical : Culture Media.
- classification : Phanerochaete, Polyporales, Shiitake Mushrooms.
- genetics : Phanerochaete, Polyporales, Shiitake Mushrooms.
- growth & development : Phanerochaete, Polyporales, Shiitake Mushrooms.
- isolation & purification : Phanerochaete, Polyporales, Shiitake Mushrooms.
- methods : Polymerase Chain Reaction, Waste Disposal, Fluid.
- Molecular Sequence Data, Mycological Typing Techniques, Sequence Analysis, DNA, Water Microbiology.
Abstract
A PCR-based method for the quantitative detection of Lentinus edodes and Trametes versicolor, two ligninolytic fungi applied for wastewater treatment and bioremediation, was developed. Genomic DNA was used to optimize a PCR method targeting the conserved copper-binding sequence of laccase genes. The method allowed the quantitative detection and differentiation of these fungi in single and defined-mixed cultures after fractionation of the PCR products by electrophoresis in agarose gels. Amplified products of about 150 bp for L. edodes, and about 200 bp for T. versicolor were purified and cloned. The PCR method showed a linear detection response in the 1.0 microg-1 ng range. The same method was tested with genomic DNA from a third fungus (Phanerochaete chrysosporium), yielding a fragment of about 400 bp. Southern-blot and DNA sequence analysis indicated that a specific PCR product was amplified from each genome, and that these corresponded to sequences of laccase genes. This PCR protocol permits the detection and differentiation of three ligninolytic fungi by amplifying DNA fragments of different sizes using a single pair of primers, without further enzymatic restriction of the PCR products. This method has potential use in the monitoring, evaluation, and improvement of fungal cultures used in wastewater treatment processes.
DOI: 10.1007/s00253-004-1795-z
PubMed: 15586279
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Poggi-Varaldo</name></author></analytic><series><title level="j">Applied microbiology and biotechnology</title><idno type="ISSN">0175-7598</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Culture Media (MeSH)</term><term>DNA, Fungal (analysis)</term><term>Laccase (genetics)</term><term>Molecular Sequence Data (MeSH)</term><term>Mycological Typing Techniques (MeSH)</term><term>Phanerochaete (classification)</term><term>Phanerochaete (genetics)</term><term>Phanerochaete (growth & development)</term><term>Phanerochaete (isolation & purification)</term><term>Polymerase Chain Reaction (methods)</term><term>Polyporales (classification)</term><term>Polyporales (genetics)</term><term>Polyporales (growth & development)</term><term>Polyporales (isolation & purification)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Shiitake Mushrooms (classification)</term><term>Shiitake Mushrooms (genetics)</term><term>Shiitake Mushrooms (growth & development)</term><term>Shiitake Mushrooms (isolation & purification)</term><term>Waste Disposal, Fluid (methods)</term><term>Water Microbiology (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (analyse)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Champignons shiitake (classification)</term><term>Champignons shiitake (croissance et développement)</term><term>Champignons shiitake (génétique)</term><term>Champignons shiitake (isolement et purification)</term><term>Données de séquences moléculaires (MeSH)</term><term>Laccase (génétique)</term><term>Microbiologie de l'eau (MeSH)</term><term>Milieux de culture (MeSH)</term><term>Phanerochaete (classification)</term><term>Phanerochaete (croissance et développement)</term><term>Phanerochaete (génétique)</term><term>Phanerochaete (isolement et purification)</term><term>Polyporales (classification)</term><term>Polyporales (croissance et développement)</term><term>Polyporales (génétique)</term><term>Polyporales (isolement et purification)</term><term>Réaction de polymérisation en chaîne (méthodes)</term><term>Techniques de typage mycologique (MeSH)</term><term>Élimination des déchets liquides (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Fungal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Laccase</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN fongique</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Phanerochaete</term><term>Polyporales</term><term>Shiitake Mushrooms</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Champignons shiitake</term><term>Phanerochaete</term><term>Polyporales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term><term>Polyporales</term><term>Shiitake Mushrooms</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phanerochaete</term><term>Polyporales</term><term>Shiitake Mushrooms</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Champignons shiitake</term><term>Laccase</term><term>Phanerochaete</term><term>Polyporales</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Phanerochaete</term><term>Polyporales</term><term>Shiitake Mushrooms</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Champignons shiitake</term><term>Phanerochaete</term><term>Polyporales</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term><term>Waste Disposal, Fluid</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Réaction de polymérisation en chaîne</term><term>Élimination des déchets liquides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Sequence Data</term><term>Mycological Typing Techniques</term><term>Sequence Analysis, DNA</term><term>Water Microbiology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Microbiologie de l'eau</term><term>Milieux de culture</term><term>Techniques de typage mycologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A PCR-based method for the quantitative detection of Lentinus edodes and Trametes versicolor, two ligninolytic fungi applied for wastewater treatment and bioremediation, was developed. Genomic DNA was used to optimize a PCR method targeting the conserved copper-binding sequence of laccase genes. The method allowed the quantitative detection and differentiation of these fungi in single and defined-mixed cultures after fractionation of the PCR products by electrophoresis in agarose gels. Amplified products of about 150 bp for L. edodes, and about 200 bp for T. versicolor were purified and cloned. The PCR method showed a linear detection response in the 1.0 microg-1 ng range. The same method was tested with genomic DNA from a third fungus (Phanerochaete chrysosporium), yielding a fragment of about 400 bp. Southern-blot and DNA sequence analysis indicated that a specific PCR product was amplified from each genome, and that these corresponded to sequences of laccase genes. This PCR protocol permits the detection and differentiation of three ligninolytic fungi by amplifying DNA fragments of different sizes using a single pair of primers, without further enzymatic restriction of the PCR products. This method has potential use in the monitoring, evaluation, and improvement of fungal cultures used in wastewater treatment processes.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15586279</PMID><DateCompleted><Year>2005</Year><Month>08</Month><Day>02</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0175-7598</ISSN><JournalIssue CitedMedium="Print"><Volume>67</Volume><Issue>4</Issue><PubDate><Year>2005</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Applied microbiology and biotechnology</Title><ISOAbbreviation>Appl Microbiol Biotechnol</ISOAbbreviation></Journal><ArticleTitle>A PCR method for the detection and differentiation of Lentinus edodes and Trametes versicolor in defined-mixed cultures used for wastewater treatment.</ArticleTitle><Pagination><MedlinePgn>524-31</MedlinePgn></Pagination><Abstract><AbstractText>A PCR-based method for the quantitative detection of Lentinus edodes and Trametes versicolor, two ligninolytic fungi applied for wastewater treatment and bioremediation, was developed. Genomic DNA was used to optimize a PCR method targeting the conserved copper-binding sequence of laccase genes. The method allowed the quantitative detection and differentiation of these fungi in single and defined-mixed cultures after fractionation of the PCR products by electrophoresis in agarose gels. Amplified products of about 150 bp for L. edodes, and about 200 bp for T. versicolor were purified and cloned. The PCR method showed a linear detection response in the 1.0 microg-1 ng range. The same method was tested with genomic DNA from a third fungus (Phanerochaete chrysosporium), yielding a fragment of about 400 bp. Southern-blot and DNA sequence analysis indicated that a specific PCR product was amplified from each genome, and that these corresponded to sequences of laccase genes. This PCR protocol permits the detection and differentiation of three ligninolytic fungi by amplifying DNA fragments of different sizes using a single pair of primers, without further enzymatic restriction of the PCR products. This method has potential use in the monitoring, evaluation, and improvement of fungal cultures used in wastewater treatment processes.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>García-Mena</LastName><ForeName>Jaime</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Departamento de Genética y Biologia Molecular, CINEVISTAV-IPN, México. jgmena@cinvestav.mx</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cano-Ramirez</LastName><ForeName>Claudia</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Garibay-Orijel</LastName><ForeName>Claudio</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Ramirez-Canseco</LastName><ForeName>Sergio</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Poggi-Varaldo</LastName><ForeName>Héctor M</ForeName><Initials>HM</Initials></Author></AuthorList><Language>eng</Language><DataBankList 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UI="D004271">DNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber><NameOfSubstance UI="D042845">Laccase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016533" MajorTopicYN="N">Mycological Typing Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020072" MajorTopicYN="N">Polyporales</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020942" MajorTopicYN="N">Shiitake Mushrooms</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014865" MajorTopicYN="N">Waste Disposal, Fluid</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014871" MajorTopicYN="Y">Water Microbiology</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year><Month>07</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2004</Year><Month>10</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2004</Year><Month>10</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>12</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2005</Year><Month>8</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>12</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15586279</ArticleId><ArticleId IdType="doi">10.1007/s00253-004-1795-z</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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