Manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium is enzymatically active and accumulates to high levels in transgenic maize seed.
Identifieur interne : 000753 ( Main/Curation ); précédent : 000752; suivant : 000754Manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium is enzymatically active and accumulates to high levels in transgenic maize seed.
Auteurs : Richard C. Clough [États-Unis] ; Kameshwari Pappu ; Kevin Thompson ; Katherine Beifuss ; Jeff Lane ; Donna E. Delaney ; Robin Harkey ; Carol Drees ; John A. Howard ; Elizabeth E. HoodSource :
- Plant biotechnology journal [ 1467-7652 ] ; 2006.
Descripteurs français
- KwdFr :
- Biotechnologie (MeSH), Cytoplasme (enzymologie), Dépollution biologique de l'environnement (MeSH), Expression des gènes (MeSH), Graines (composition chimique), Graines (génétique), Graines (métabolisme), Gènes fongiques (MeSH), Lignine (métabolisme), Paroi cellulaire (enzymologie), Peroxidases (génétique), Peroxidases (métabolisme), Phanerochaete (enzymologie), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Transformation génétique (MeSH), Zea mays (composition chimique), Zea mays (génétique), Zea mays (métabolisme).
- MESH :
- composition chimique : Graines, Zea mays.
- enzymologie : Cytoplasme, Paroi cellulaire, Phanerochaete.
- génétique : Graines, Peroxidases, Protéines fongiques, Zea mays.
- métabolisme : Graines, Lignine, Peroxidases, Protéines fongiques, Zea mays.
- Biotechnologie, Dépollution biologique de l'environnement, Expression des gènes, Gènes fongiques, Transformation génétique.
English descriptors
- KwdEn :
- Biodegradation, Environmental (MeSH), Biotechnology (MeSH), Cell Wall (enzymology), Cytoplasm (enzymology), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression (MeSH), Genes, Fungal (MeSH), Lignin (metabolism), Peroxidases (genetics), Peroxidases (metabolism), Phanerochaete (enzymology), Seeds (chemistry), Seeds (genetics), Seeds (metabolism), Transformation, Genetic (MeSH), Zea mays (chemistry), Zea mays (genetics), Zea mays (metabolism).
- MESH :
- chemical , genetics : Fungal Proteins, Peroxidases.
- chemistry : Seeds, Zea mays.
- enzymology : Cell Wall, Cytoplasm, Phanerochaete.
- genetics : Seeds, Zea mays.
- chemical , metabolism : Fungal Proteins, Lignin, Peroxidases, Seeds, Zea mays.
- Biodegradation, Environmental, Biotechnology, Gene Expression, Genes, Fungal, Transformation, Genetic.
Abstract
Manganese peroxidase (MnP) has been implicated in lignin degradation and thus has potential applications in pulp and paper bleaching, enzymatic remediation and the textile industry. Transgenic plants are an emerging protein expression platform that offer many advantages over traditional systems, in particular their potential for large-scale industrial enzyme production. Several plant expression vectors were created to evaluate the accumulation of MnP from the wood-rot fungus Phanerochaete chrysosporium in maize seed. We showed that cell wall targeting yielded full-length MnP, whereas cytoplasmic localization resulted in multiple truncated peroxidase polypeptides as detected by immunoblot analysis. In addition, the use of a seed-preferred promoter dramatically increased the expression levels and reduced the negative effects on plant health. Multiple independent transgenic lines were backcrossed with elite inbred corn lines for several generations with the maintenance of high-level expression, indicating genetic stability of the transgene.
DOI: 10.1111/j.1467-7652.2005.00157.x
PubMed: 17177785
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<term>Biotechnology (MeSH)</term>
<term>Cell Wall (enzymology)</term>
<term>Cytoplasm (enzymology)</term>
<term>Fungal Proteins (genetics)</term>
<term>Fungal Proteins (metabolism)</term>
<term>Gene Expression (MeSH)</term>
<term>Genes, Fungal (MeSH)</term>
<term>Lignin (metabolism)</term>
<term>Peroxidases (genetics)</term>
<term>Peroxidases (metabolism)</term>
<term>Phanerochaete (enzymology)</term>
<term>Seeds (chemistry)</term>
<term>Seeds (genetics)</term>
<term>Seeds (metabolism)</term>
<term>Transformation, Genetic (MeSH)</term>
<term>Zea mays (chemistry)</term>
<term>Zea mays (genetics)</term>
<term>Zea mays (metabolism)</term>
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<term>Cytoplasme (enzymologie)</term>
<term>Dépollution biologique de l'environnement (MeSH)</term>
<term>Expression des gènes (MeSH)</term>
<term>Graines (composition chimique)</term>
<term>Graines (génétique)</term>
<term>Graines (métabolisme)</term>
<term>Gènes fongiques (MeSH)</term>
<term>Lignine (métabolisme)</term>
<term>Paroi cellulaire (enzymologie)</term>
<term>Peroxidases (génétique)</term>
<term>Peroxidases (métabolisme)</term>
<term>Phanerochaete (enzymologie)</term>
<term>Protéines fongiques (génétique)</term>
<term>Protéines fongiques (métabolisme)</term>
<term>Transformation génétique (MeSH)</term>
<term>Zea mays (composition chimique)</term>
<term>Zea mays (génétique)</term>
<term>Zea mays (métabolisme)</term>
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<term>Peroxidases</term>
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<term>Zea mays</term>
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<term>Zea mays</term>
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<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Cytoplasme</term>
<term>Paroi cellulaire</term>
<term>Phanerochaete</term>
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<term>Phanerochaete</term>
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<term>Zea mays</term>
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<term>Lignin</term>
<term>Peroxidases</term>
<term>Seeds</term>
<term>Zea mays</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Graines</term>
<term>Lignine</term>
<term>Peroxidases</term>
<term>Protéines fongiques</term>
<term>Zea mays</term>
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<term>Biotechnology</term>
<term>Gene Expression</term>
<term>Genes, Fungal</term>
<term>Transformation, Genetic</term>
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<term>Dépollution biologique de l'environnement</term>
<term>Expression des gènes</term>
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<front><div type="abstract" xml:lang="en">Manganese peroxidase (MnP) has been implicated in lignin degradation and thus has potential applications in pulp and paper bleaching, enzymatic remediation and the textile industry. Transgenic plants are an emerging protein expression platform that offer many advantages over traditional systems, in particular their potential for large-scale industrial enzyme production. Several plant expression vectors were created to evaluate the accumulation of MnP from the wood-rot fungus Phanerochaete chrysosporium in maize seed. We showed that cell wall targeting yielded full-length MnP, whereas cytoplasmic localization resulted in multiple truncated peroxidase polypeptides as detected by immunoblot analysis. In addition, the use of a seed-preferred promoter dramatically increased the expression levels and reduced the negative effects on plant health. Multiple independent transgenic lines were backcrossed with elite inbred corn lines for several generations with the maintenance of high-level expression, indicating genetic stability of the transgene.</div>
</front>
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<Abstract><AbstractText>Manganese peroxidase (MnP) has been implicated in lignin degradation and thus has potential applications in pulp and paper bleaching, enzymatic remediation and the textile industry. Transgenic plants are an emerging protein expression platform that offer many advantages over traditional systems, in particular their potential for large-scale industrial enzyme production. Several plant expression vectors were created to evaluate the accumulation of MnP from the wood-rot fungus Phanerochaete chrysosporium in maize seed. We showed that cell wall targeting yielded full-length MnP, whereas cytoplasmic localization resulted in multiple truncated peroxidase polypeptides as detected by immunoblot analysis. In addition, the use of a seed-preferred promoter dramatically increased the expression levels and reduced the negative effects on plant health. Multiple independent transgenic lines were backcrossed with elite inbred corn lines for several generations with the maintenance of high-level expression, indicating genetic stability of the transgene.</AbstractText>
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