Manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium is enzymatically active and accumulates to high levels in transgenic maize seed.
Identifieur interne : 000753 ( Main/Curation ); précédent : 000752; suivant : 000754Manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium is enzymatically active and accumulates to high levels in transgenic maize seed.
Auteurs : Richard C. Clough [États-Unis] ; Kameshwari Pappu ; Kevin Thompson ; Katherine Beifuss ; Jeff Lane ; Donna E. Delaney ; Robin Harkey ; Carol Drees ; John A. Howard ; Elizabeth E. HoodSource :
- Plant biotechnology journal [ 1467-7652 ] ; 2006.
Descripteurs français
- KwdFr :
- Biotechnologie (MeSH), Cytoplasme (enzymologie), Dépollution biologique de l'environnement (MeSH), Expression des gènes (MeSH), Graines (composition chimique), Graines (génétique), Graines (métabolisme), Gènes fongiques (MeSH), Lignine (métabolisme), Paroi cellulaire (enzymologie), Peroxidases (génétique), Peroxidases (métabolisme), Phanerochaete (enzymologie), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Transformation génétique (MeSH), Zea mays (composition chimique), Zea mays (génétique), Zea mays (métabolisme).
- MESH :
- composition chimique : Graines, Zea mays.
- enzymologie : Cytoplasme, Paroi cellulaire, Phanerochaete.
- génétique : Graines, Peroxidases, Protéines fongiques, Zea mays.
- métabolisme : Graines, Lignine, Peroxidases, Protéines fongiques, Zea mays.
- Biotechnologie, Dépollution biologique de l'environnement, Expression des gènes, Gènes fongiques, Transformation génétique.
English descriptors
- KwdEn :
- Biodegradation, Environmental (MeSH), Biotechnology (MeSH), Cell Wall (enzymology), Cytoplasm (enzymology), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression (MeSH), Genes, Fungal (MeSH), Lignin (metabolism), Peroxidases (genetics), Peroxidases (metabolism), Phanerochaete (enzymology), Seeds (chemistry), Seeds (genetics), Seeds (metabolism), Transformation, Genetic (MeSH), Zea mays (chemistry), Zea mays (genetics), Zea mays (metabolism).
- MESH :
- chemical , genetics : Fungal Proteins, Peroxidases.
- chemistry : Seeds, Zea mays.
- enzymology : Cell Wall, Cytoplasm, Phanerochaete.
- genetics : Seeds, Zea mays.
- chemical , metabolism : Fungal Proteins, Lignin, Peroxidases, Seeds, Zea mays.
- Biodegradation, Environmental, Biotechnology, Gene Expression, Genes, Fungal, Transformation, Genetic.
Abstract
Manganese peroxidase (MnP) has been implicated in lignin degradation and thus has potential applications in pulp and paper bleaching, enzymatic remediation and the textile industry. Transgenic plants are an emerging protein expression platform that offer many advantages over traditional systems, in particular their potential for large-scale industrial enzyme production. Several plant expression vectors were created to evaluate the accumulation of MnP from the wood-rot fungus Phanerochaete chrysosporium in maize seed. We showed that cell wall targeting yielded full-length MnP, whereas cytoplasmic localization resulted in multiple truncated peroxidase polypeptides as detected by immunoblot analysis. In addition, the use of a seed-preferred promoter dramatically increased the expression levels and reduced the negative effects on plant health. Multiple independent transgenic lines were backcrossed with elite inbred corn lines for several generations with the maintenance of high-level expression, indicating genetic stability of the transgene.
DOI: 10.1111/j.1467-7652.2005.00157.x
PubMed: 17177785
Links toward previous steps (curation, corpus...)
- to stream Main, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000753
Links to Exploration step
pubmed:17177785Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium is enzymatically active and accumulates to high levels in transgenic maize seed.</title><author><name sortKey="Clough, Richard C" sort="Clough, Richard C" uniqKey="Clough R" first="Richard C" last="Clough">Richard C. Clough</name><affiliation wicri:level="1"><nlm:affiliation>ProdiGene, Inc., 101 Gateway Blvd., Suite 100, College Station, TX 77845, USA. clough@cox-internet.com</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>ProdiGene, Inc., 101 Gateway Blvd., Suite 100, College Station, TX 77845</wicri:regionArea></affiliation></author><author><name sortKey="Pappu, Kameshwari" sort="Pappu, Kameshwari" uniqKey="Pappu K" first="Kameshwari" last="Pappu">Kameshwari Pappu</name></author><author><name sortKey="Thompson, Kevin" sort="Thompson, Kevin" uniqKey="Thompson K" first="Kevin" last="Thompson">Kevin Thompson</name></author><author><name sortKey="Beifuss, Katherine" sort="Beifuss, Katherine" uniqKey="Beifuss K" first="Katherine" last="Beifuss">Katherine Beifuss</name></author><author><name sortKey="Lane, Jeff" sort="Lane, Jeff" uniqKey="Lane J" first="Jeff" last="Lane">Jeff Lane</name></author><author><name sortKey="Delaney, Donna E" sort="Delaney, Donna E" uniqKey="Delaney D" first="Donna E" last="Delaney">Donna E. Delaney</name></author><author><name sortKey="Harkey, Robin" sort="Harkey, Robin" uniqKey="Harkey R" first="Robin" last="Harkey">Robin Harkey</name></author><author><name sortKey="Drees, Carol" sort="Drees, Carol" uniqKey="Drees C" first="Carol" last="Drees">Carol Drees</name></author><author><name sortKey="Howard, John A" sort="Howard, John A" uniqKey="Howard J" first="John A" last="Howard">John A. Howard</name></author><author><name sortKey="Hood, Elizabeth E" sort="Hood, Elizabeth E" uniqKey="Hood E" first="Elizabeth E" last="Hood">Elizabeth E. Hood</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:17177785</idno><idno type="pmid">17177785</idno><idno type="doi">10.1111/j.1467-7652.2005.00157.x</idno><idno type="wicri:Area/Main/Corpus">000753</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000753</idno><idno type="wicri:Area/Main/Curation">000753</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000753</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium is enzymatically active and accumulates to high levels in transgenic maize seed.</title><author><name sortKey="Clough, Richard C" sort="Clough, Richard C" uniqKey="Clough R" first="Richard C" last="Clough">Richard C. Clough</name><affiliation wicri:level="1"><nlm:affiliation>ProdiGene, Inc., 101 Gateway Blvd., Suite 100, College Station, TX 77845, USA. clough@cox-internet.com</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>ProdiGene, Inc., 101 Gateway Blvd., Suite 100, College Station, TX 77845</wicri:regionArea></affiliation></author><author><name sortKey="Pappu, Kameshwari" sort="Pappu, Kameshwari" uniqKey="Pappu K" first="Kameshwari" last="Pappu">Kameshwari Pappu</name></author><author><name sortKey="Thompson, Kevin" sort="Thompson, Kevin" uniqKey="Thompson K" first="Kevin" last="Thompson">Kevin Thompson</name></author><author><name sortKey="Beifuss, Katherine" sort="Beifuss, Katherine" uniqKey="Beifuss K" first="Katherine" last="Beifuss">Katherine Beifuss</name></author><author><name sortKey="Lane, Jeff" sort="Lane, Jeff" uniqKey="Lane J" first="Jeff" last="Lane">Jeff Lane</name></author><author><name sortKey="Delaney, Donna E" sort="Delaney, Donna E" uniqKey="Delaney D" first="Donna E" last="Delaney">Donna E. Delaney</name></author><author><name sortKey="Harkey, Robin" sort="Harkey, Robin" uniqKey="Harkey R" first="Robin" last="Harkey">Robin Harkey</name></author><author><name sortKey="Drees, Carol" sort="Drees, Carol" uniqKey="Drees C" first="Carol" last="Drees">Carol Drees</name></author><author><name sortKey="Howard, John A" sort="Howard, John A" uniqKey="Howard J" first="John A" last="Howard">John A. Howard</name></author><author><name sortKey="Hood, Elizabeth E" sort="Hood, Elizabeth E" uniqKey="Hood E" first="Elizabeth E" last="Hood">Elizabeth E. Hood</name></author></analytic><series><title level="j">Plant biotechnology journal</title><idno type="eISSN">1467-7652</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodegradation, Environmental (MeSH)</term><term>Biotechnology (MeSH)</term><term>Cell Wall (enzymology)</term><term>Cytoplasm (enzymology)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Gene Expression (MeSH)</term><term>Genes, Fungal (MeSH)</term><term>Lignin (metabolism)</term><term>Peroxidases (genetics)</term><term>Peroxidases (metabolism)</term><term>Phanerochaete (enzymology)</term><term>Seeds (chemistry)</term><term>Seeds (genetics)</term><term>Seeds (metabolism)</term><term>Transformation, Genetic (MeSH)</term><term>Zea mays (chemistry)</term><term>Zea mays (genetics)</term><term>Zea mays (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biotechnologie (MeSH)</term><term>Cytoplasme (enzymologie)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Expression des gènes (MeSH)</term><term>Graines (composition chimique)</term><term>Graines (génétique)</term><term>Graines (métabolisme)</term><term>Gènes fongiques (MeSH)</term><term>Lignine (métabolisme)</term><term>Paroi cellulaire (enzymologie)</term><term>Peroxidases (génétique)</term><term>Peroxidases (métabolisme)</term><term>Phanerochaete (enzymologie)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Transformation génétique (MeSH)</term><term>Zea mays (composition chimique)</term><term>Zea mays (génétique)</term><term>Zea mays (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Seeds</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Graines</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Cytoplasme</term><term>Paroi cellulaire</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Cell Wall</term><term>Cytoplasm</term><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Seeds</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Graines</term><term>Peroxidases</term><term>Protéines fongiques</term><term>Zea mays</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term><term>Lignin</term><term>Peroxidases</term><term>Seeds</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Graines</term><term>Lignine</term><term>Peroxidases</term><term>Protéines fongiques</term><term>Zea mays</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Biotechnology</term><term>Gene Expression</term><term>Genes, Fungal</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biotechnologie</term><term>Dépollution biologique de l'environnement</term><term>Expression des gènes</term><term>Gènes fongiques</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Manganese peroxidase (MnP) has been implicated in lignin degradation and thus has potential applications in pulp and paper bleaching, enzymatic remediation and the textile industry. Transgenic plants are an emerging protein expression platform that offer many advantages over traditional systems, in particular their potential for large-scale industrial enzyme production. Several plant expression vectors were created to evaluate the accumulation of MnP from the wood-rot fungus Phanerochaete chrysosporium in maize seed. We showed that cell wall targeting yielded full-length MnP, whereas cytoplasmic localization resulted in multiple truncated peroxidase polypeptides as detected by immunoblot analysis. In addition, the use of a seed-preferred promoter dramatically increased the expression levels and reduced the negative effects on plant health. Multiple independent transgenic lines were backcrossed with elite inbred corn lines for several generations with the maintenance of high-level expression, indicating genetic stability of the transgene.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17177785</PMID><DateCompleted><Year>2007</Year><Month>01</Month><Day>26</Day></DateCompleted><DateRevised><Year>2007</Year><Month>02</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1467-7652</ISSN><JournalIssue CitedMedium="Internet"><Volume>4</Volume><Issue>1</Issue><PubDate><Year>2006</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant biotechnology journal</Title><ISOAbbreviation>Plant Biotechnol J</ISOAbbreviation></Journal><ArticleTitle>Manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium is enzymatically active and accumulates to high levels in transgenic maize seed.</ArticleTitle><Pagination><MedlinePgn>53-62</MedlinePgn></Pagination><Abstract><AbstractText>Manganese peroxidase (MnP) has been implicated in lignin degradation and thus has potential applications in pulp and paper bleaching, enzymatic remediation and the textile industry. Transgenic plants are an emerging protein expression platform that offer many advantages over traditional systems, in particular their potential for large-scale industrial enzyme production. Several plant expression vectors were created to evaluate the accumulation of MnP from the wood-rot fungus Phanerochaete chrysosporium in maize seed. We showed that cell wall targeting yielded full-length MnP, whereas cytoplasmic localization resulted in multiple truncated peroxidase polypeptides as detected by immunoblot analysis. In addition, the use of a seed-preferred promoter dramatically increased the expression levels and reduced the negative effects on plant health. Multiple independent transgenic lines were backcrossed with elite inbred corn lines for several generations with the maintenance of high-level expression, indicating genetic stability of the transgene.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Clough</LastName><ForeName>Richard C</ForeName><Initials>RC</Initials><AffiliationInfo><Affiliation>ProdiGene, Inc., 101 Gateway Blvd., Suite 100, College Station, TX 77845, USA. clough@cox-internet.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pappu</LastName><ForeName>Kameshwari</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Thompson</LastName><ForeName>Kevin</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Beifuss</LastName><ForeName>Katherine</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Lane</LastName><ForeName>Jeff</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Delaney</LastName><ForeName>Donna E</ForeName><Initials>DE</Initials></Author><Author ValidYN="Y"><LastName>Harkey</LastName><ForeName>Robin</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Drees</LastName><ForeName>Carol</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Howard</LastName><ForeName>John A</ForeName><Initials>JA</Initials></Author><Author ValidYN="Y"><LastName>Hood</LastName><ForeName>Elizabeth E</ForeName><Initials>EE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Biotechnol J</MedlineTA><NlmUniqueID>101201889</NlmUniqueID><ISSNLinking>1467-7644</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003593" MajorTopicYN="N">Cytoplasm</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003313" MajorTopicYN="N">Zea mays</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>12</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>1</Month><Day>27</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>12</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17177785</ArticleId><ArticleId IdType="pii">PBI157</ArticleId><ArticleId IdType="doi">10.1111/j.1467-7652.2005.00157.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000753 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 000753 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhanerochaeteV1
|flux= Main
|étape= Curation
|type= RBID
|clé= pubmed:17177785
|texte= Manganese peroxidase from the white-rot fungus Phanerochaete chrysosporium is enzymatically active and accumulates to high levels in transgenic maize seed.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:17177785" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \
| NlmPubMed2Wicri -a PhanerochaeteV1
| This area was generated with Dilib version V0.6.37. |  |