[Breeding and characterization of laccase-producing Phanerochaete chrysosporium mutant resistant to nutritional repression].
Identifieur interne : 000493 ( Main/Curation ); précédent : 000492; suivant : 000494[Breeding and characterization of laccase-producing Phanerochaete chrysosporium mutant resistant to nutritional repression].
Auteurs : Ailian Qiu [République populaire de Chine] ; Wenyan Li ; Yaotong Zheng ; Xiaojing Fan ; Youxian Ye ; Yan MengSource :
- Wei sheng wu xue bao = Acta microbiologica Sinica [ 0001-6209 ] ; 2011.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : Laccase.
- croissance et développement : Phanerochaete.
- enzymologie : Phanerochaete.
- génétique : Phanerochaete.
- isolement et purification : Phanerochaete.
- métabolisme : Azote, Guaïacol, Peroxidases.
- Aliments, Mutagenèse.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Laccase.
- chemical , metabolism : Guaiacol, Nitrogen, Peroxidases.
- enzymology : Phanerochaete.
- genetics : Phanerochaete.
- growth & development : Phanerochaete.
- isolation & purification : Phanerochaete.
- Food, Mutagenesis.
Abstract
OBJECTIVE
To screen Phanerochaete chrysosporium mutants resisting nutritional repression and to characterize laccase produced by the mutants.
METHODS
We used repeated UV mutagenesis and screened the mutant strains by using the guaiacol nitrogen sufficient differential medium. We characterized enzymes production mechanism of the nutritional regulation through comparing the differences of cell growth and enzyme-production kinetics under different nutritional conditions; We validated production of laccase by Phanerochaete chrysosporium through measurements of the heat treatment, removal of manganese ion and addition of the catalase.
RESULTS
Three different methods were validated that both strains of pcR5305 and pcR5324 can produce laccase under the nitrogen limitation (N-L) and nitrogen sufficient (N-S) conditions. Under the N-L conditions, pcR5305 can produce 203.5 U/L laccase and pcR5324 can produce 187.6 U/L laccase; Under the N-S conditions, pcR5305 can produce 220.6 U/L laccase and pcR5324 can produce 183.9 U/L laccase. The original strain pc530 only can produce very little laccase under either conditions. The laccase-production regulation mechanisms of the two strains are different: Production of laccase and the cell growth by pcR5305 are in synchronism. However production of the laccase by pcR5324 is repressed by nutrition. Both strains have the capacity of resisting nutritional repression and produce lignin peroxidase and manganese peroxidase with high yield. (LiP 1343.2, MnP 252.2 U/L and LiP 1169.5, MnP 172.4 U/L respectively).
CONCLUSION
The mutants of Phanerochaete chrysosporium can produce laccase. At same time they showed the capacity of resisting nutritional repression and production of laccase, lignin peroxidase and manganese peroxidase. Our results possess high value for production, application and fundamental research. We provided new strains and established a very good foundation for the further research of metabolic regulation of ligninolytic enzymes production.
PubMed: 21604549
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Breeding and characterization of laccase-producing Phanerochaete chrysosporium mutant resistant to nutritional repression].</title><author><name sortKey="Qiu, Ailian" sort="Qiu, Ailian" uniqKey="Qiu A" first="Ailian" last="Qiu">Ailian Qiu</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 35002, China. qiual888@sina.com</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 35002</wicri:regionArea></affiliation></author><author><name sortKey="Li, Wenyan" sort="Li, Wenyan" uniqKey="Li W" first="Wenyan" last="Li">Wenyan Li</name></author><author><name sortKey="Zheng, Yaotong" sort="Zheng, Yaotong" uniqKey="Zheng Y" first="Yaotong" last="Zheng">Yaotong Zheng</name></author><author><name sortKey="Fan, Xiaojing" sort="Fan, Xiaojing" uniqKey="Fan X" first="Xiaojing" last="Fan">Xiaojing Fan</name></author><author><name sortKey="Ye, Youxian" sort="Ye, Youxian" uniqKey="Ye Y" first="Youxian" last="Ye">Youxian Ye</name></author><author><name sortKey="Meng, Yan" sort="Meng, Yan" uniqKey="Meng Y" first="Yan" last="Meng">Yan Meng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21604549</idno><idno type="pmid">21604549</idno><idno type="wicri:Area/Main/Corpus">000493</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000493</idno><idno type="wicri:Area/Main/Curation">000493</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000493</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Breeding and characterization of laccase-producing Phanerochaete chrysosporium mutant resistant to nutritional repression].</title><author><name sortKey="Qiu, Ailian" sort="Qiu, Ailian" uniqKey="Qiu A" first="Ailian" last="Qiu">Ailian Qiu</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 35002, China. qiual888@sina.com</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 35002</wicri:regionArea></affiliation></author><author><name sortKey="Li, Wenyan" sort="Li, Wenyan" uniqKey="Li W" first="Wenyan" last="Li">Wenyan Li</name></author><author><name sortKey="Zheng, Yaotong" sort="Zheng, Yaotong" uniqKey="Zheng Y" first="Yaotong" last="Zheng">Yaotong Zheng</name></author><author><name sortKey="Fan, Xiaojing" sort="Fan, Xiaojing" uniqKey="Fan X" first="Xiaojing" last="Fan">Xiaojing Fan</name></author><author><name sortKey="Ye, Youxian" sort="Ye, Youxian" uniqKey="Ye Y" first="Youxian" last="Ye">Youxian Ye</name></author><author><name sortKey="Meng, Yan" sort="Meng, Yan" uniqKey="Meng Y" first="Yan" last="Meng">Yan Meng</name></author></analytic><series><title level="j">Wei sheng wu xue bao = Acta microbiologica Sinica</title><idno type="ISSN">0001-6209</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Food (MeSH)</term><term>Guaiacol (metabolism)</term><term>Laccase (biosynthesis)</term><term>Mutagenesis (MeSH)</term><term>Nitrogen (metabolism)</term><term>Peroxidases (metabolism)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (genetics)</term><term>Phanerochaete (growth & development)</term><term>Phanerochaete (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aliments (MeSH)</term><term>Azote (métabolisme)</term><term>Guaïacol (métabolisme)</term><term>Laccase (biosynthèse)</term><term>Mutagenèse (MeSH)</term><term>Peroxidases (métabolisme)</term><term>Phanerochaete (croissance et développement)</term><term>Phanerochaete (enzymologie)</term><term>Phanerochaete (génétique)</term><term>Phanerochaete (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Laccase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Guaiacol</term><term>Nitrogen</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Laccase</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Azote</term><term>Guaïacol</term><term>Peroxidases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Food</term><term>Mutagenesis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Aliments</term><term>Mutagenèse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>To screen Phanerochaete chrysosporium mutants resisting nutritional repression and to characterize laccase produced by the mutants.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>We used repeated UV mutagenesis and screened the mutant strains by using the guaiacol nitrogen sufficient differential medium. We characterized enzymes production mechanism of the nutritional regulation through comparing the differences of cell growth and enzyme-production kinetics under different nutritional conditions; We validated production of laccase by Phanerochaete chrysosporium through measurements of the heat treatment, removal of manganese ion and addition of the catalase.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Three different methods were validated that both strains of pcR5305 and pcR5324 can produce laccase under the nitrogen limitation (N-L) and nitrogen sufficient (N-S) conditions. Under the N-L conditions, pcR5305 can produce 203.5 U/L laccase and pcR5324 can produce 187.6 U/L laccase; Under the N-S conditions, pcR5305 can produce 220.6 U/L laccase and pcR5324 can produce 183.9 U/L laccase. The original strain pc530 only can produce very little laccase under either conditions. The laccase-production regulation mechanisms of the two strains are different: Production of laccase and the cell growth by pcR5305 are in synchronism. However production of the laccase by pcR5324 is repressed by nutrition. Both strains have the capacity of resisting nutritional repression and produce lignin peroxidase and manganese peroxidase with high yield. (LiP 1343.2, MnP 252.2 U/L and LiP 1169.5, MnP 172.4 U/L respectively).</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>The mutants of Phanerochaete chrysosporium can produce laccase. At same time they showed the capacity of resisting nutritional repression and production of laccase, lignin peroxidase and manganese peroxidase. Our results possess high value for production, application and fundamental research. We provided new strains and established a very good foundation for the further research of metabolic regulation of ligninolytic enzymes production.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21604549</PMID><DateCompleted><Year>2011</Year><Month>06</Month><Day>09</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0001-6209</ISSN><JournalIssue CitedMedium="Print"><Volume>51</Volume><Issue>3</Issue><PubDate><Year>2011</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Wei sheng wu xue bao = Acta microbiologica Sinica</Title><ISOAbbreviation>Wei Sheng Wu Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Breeding and characterization of laccase-producing Phanerochaete chrysosporium mutant resistant to nutritional repression].</ArticleTitle><Pagination><MedlinePgn>352-9</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To screen Phanerochaete chrysosporium mutants resisting nutritional repression and to characterize laccase produced by the mutants.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">We used repeated UV mutagenesis and screened the mutant strains by using the guaiacol nitrogen sufficient differential medium. We characterized enzymes production mechanism of the nutritional regulation through comparing the differences of cell growth and enzyme-production kinetics under different nutritional conditions; We validated production of laccase by Phanerochaete chrysosporium through measurements of the heat treatment, removal of manganese ion and addition of the catalase.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Three different methods were validated that both strains of pcR5305 and pcR5324 can produce laccase under the nitrogen limitation (N-L) and nitrogen sufficient (N-S) conditions. Under the N-L conditions, pcR5305 can produce 203.5 U/L laccase and pcR5324 can produce 187.6 U/L laccase; Under the N-S conditions, pcR5305 can produce 220.6 U/L laccase and pcR5324 can produce 183.9 U/L laccase. The original strain pc530 only can produce very little laccase under either conditions. The laccase-production regulation mechanisms of the two strains are different: Production of laccase and the cell growth by pcR5305 are in synchronism. However production of the laccase by pcR5324 is repressed by nutrition. Both strains have the capacity of resisting nutritional repression and produce lignin peroxidase and manganese peroxidase with high yield. (LiP 1343.2, MnP 252.2 U/L and LiP 1169.5, MnP 172.4 U/L respectively).</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The mutants of Phanerochaete chrysosporium can produce laccase. At same time they showed the capacity of resisting nutritional repression and production of laccase, lignin peroxidase and manganese peroxidase. Our results possess high value for production, application and fundamental research. We provided new strains and established a very good foundation for the further research of metabolic regulation of ligninolytic enzymes production.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Qiu</LastName><ForeName>Ailian</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 35002, China. qiual888@sina.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Wenyan</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Yaotong</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>Xiaojing</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Ye</LastName><ForeName>Youxian</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Meng</LastName><ForeName>Yan</ForeName><Initials>Y</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Wei Sheng Wu Xue Bao</MedlineTA><NlmUniqueID>21610860R</NlmUniqueID><ISSNLinking>0001-6209</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>6JKA7MAH9C</RegistryNumber><NameOfSubstance UI="D006139">Guaiacol</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber><NameOfSubstance UI="D042845">Laccase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="C042858">lignin peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005502" MajorTopicYN="N">Food</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006139" MajorTopicYN="N">Guaiacol</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016296" MajorTopicYN="N">Mutagenesis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>5</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>5</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>6</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21604549</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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