Real-time PCR investigation of the dynamic expression of three "RNA processing and modification" genes of Phanerochaete chrysosporium exposed to lead.
Identifieur interne : 000392 ( Main/Curation ); précédent : 000391; suivant : 000393Real-time PCR investigation of the dynamic expression of three "RNA processing and modification" genes of Phanerochaete chrysosporium exposed to lead.
Auteurs : Elif Tekin [Turquie] ; Bulent Icgen ; Gulay OzcengizSource :
- Bulletin of environmental contamination and toxicology [ 1432-0800 ] ; 2013.
Descripteurs français
- KwdFr :
- ARN messager (métabolisme), Exposition environnementale (MeSH), Phanerochaete (effets des médicaments et des substances chimiques), Phanerochaete (génétique), Phanerochaete (métabolisme), Plomb (toxicité), Polluants environnementaux (toxicité), Protéines de liaison à l'ARN (génétique), Protéines de liaison à l'ARN (métabolisme), Réaction de polymérisation en chaine en temps réel (MeSH), Régulation de l'expression des gènes (effets des médicaments et des substances chimiques), Surveillance de l'environnement (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Phanerochaete, Régulation de l'expression des gènes.
- génétique : Phanerochaete, Protéines de liaison à l'ARN.
- métabolisme : ARN messager, Phanerochaete, Protéines de liaison à l'ARN.
- toxicité : Plomb, Polluants environnementaux.
- Exposition environnementale, Réaction de polymérisation en chaine en temps réel, Surveillance de l'environnement.
English descriptors
- KwdEn :
- Environmental Exposure (MeSH), Environmental Monitoring (MeSH), Environmental Pollutants (toxicity), Gene Expression Regulation (drug effects), Lead (toxicity), Phanerochaete (drug effects), Phanerochaete (genetics), Phanerochaete (metabolism), RNA, Messenger (metabolism), RNA-Binding Proteins (genetics), RNA-Binding Proteins (metabolism), Real-Time Polymerase Chain Reaction (MeSH).
- MESH :
- chemical , genetics : RNA-Binding Proteins.
- chemical , metabolism : RNA, Messenger, RNA-Binding Proteins.
- chemical , toxicity : Environmental Pollutants, Lead.
- drug effects : Gene Expression Regulation, Phanerochaete.
- genetics : Phanerochaete.
- metabolism : Phanerochaete.
- Environmental Exposure, Environmental Monitoring, Real-Time Polymerase Chain Reaction.
Abstract
The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 μM lead, respectively. 50 and 100 μM lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the expression of ATP-dependent RNA helicase. Polyadenylate-binding protein mRNA levels did not reveal any significant increase when cells exposed to the concentrations of lead employed. However, the mRNA level of polyadenylate-binding protein and splicing factor RNPS1 within a period of 1 and 2 h temporal exposure to 100 μM lead showed 2.5 (p < 0.001) and 3.4-fold (p < 0.001) increase, respectively. Expression level of ATP-dependent RNA helicase was not affected from the period of exposure to this metal.
DOI: 10.1007/s00128-012-0886-2
PubMed: 23160747
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qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Phanerochaete</term><term>Protéines de liaison à l'ARN</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Plomb</term><term>Polluants environnementaux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Environmental Exposure</term><term>Environmental Monitoring</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Exposition environnementale</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Surveillance de l'environnement</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 μM lead, respectively. 50 and 100 μM lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the expression of ATP-dependent RNA helicase. Polyadenylate-binding protein mRNA levels did not reveal any significant increase when cells exposed to the concentrations of lead employed. However, the mRNA level of polyadenylate-binding protein and splicing factor RNPS1 within a period of 1 and 2 h temporal exposure to 100 μM lead showed 2.5 (p < 0.001) and 3.4-fold (p < 0.001) increase, respectively. Expression level of ATP-dependent RNA helicase was not affected from the period of exposure to this metal.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23160747</PMID><DateCompleted><Year>2013</Year><Month>06</Month><Day>17</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0800</ISSN><JournalIssue CitedMedium="Internet"><Volume>90</Volume><Issue>1</Issue><PubDate><Year>2013</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Bulletin of environmental contamination and toxicology</Title><ISOAbbreviation>Bull Environ Contam Toxicol</ISOAbbreviation></Journal><ArticleTitle>Real-time PCR investigation of the dynamic expression of three "RNA processing and modification" genes of Phanerochaete chrysosporium exposed to lead.</ArticleTitle><Pagination><MedlinePgn>27-33</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00128-012-0886-2</ELocationID><Abstract><AbstractText>The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 μM lead, respectively. 50 and 100 μM lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the expression of ATP-dependent RNA helicase. Polyadenylate-binding protein mRNA levels did not reveal any significant increase when cells exposed to the concentrations of lead employed. However, the mRNA level of polyadenylate-binding protein and splicing factor RNPS1 within a period of 1 and 2 h temporal exposure to 100 μM lead showed 2.5 (p < 0.001) and 3.4-fold (p < 0.001) increase, respectively. 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