Real-time PCR investigation of the dynamic expression of three "RNA processing and modification" genes of Phanerochaete chrysosporium exposed to lead.
Identifieur interne : 000392 ( Main/Curation ); précédent : 000391; suivant : 000393Real-time PCR investigation of the dynamic expression of three "RNA processing and modification" genes of Phanerochaete chrysosporium exposed to lead.
Auteurs : Elif Tekin [Turquie] ; Bulent Icgen ; Gulay OzcengizSource :
- Bulletin of environmental contamination and toxicology [ 1432-0800 ] ; 2013.
Descripteurs français
- KwdFr :
- ARN messager (métabolisme), Exposition environnementale (MeSH), Phanerochaete (effets des médicaments et des substances chimiques), Phanerochaete (génétique), Phanerochaete (métabolisme), Plomb (toxicité), Polluants environnementaux (toxicité), Protéines de liaison à l'ARN (génétique), Protéines de liaison à l'ARN (métabolisme), Réaction de polymérisation en chaine en temps réel (MeSH), Régulation de l'expression des gènes (effets des médicaments et des substances chimiques), Surveillance de l'environnement (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Phanerochaete, Régulation de l'expression des gènes.
- génétique : Phanerochaete, Protéines de liaison à l'ARN.
- métabolisme : ARN messager, Phanerochaete, Protéines de liaison à l'ARN.
- toxicité : Plomb, Polluants environnementaux.
- Exposition environnementale, Réaction de polymérisation en chaine en temps réel, Surveillance de l'environnement.
English descriptors
- KwdEn :
- Environmental Exposure (MeSH), Environmental Monitoring (MeSH), Environmental Pollutants (toxicity), Gene Expression Regulation (drug effects), Lead (toxicity), Phanerochaete (drug effects), Phanerochaete (genetics), Phanerochaete (metabolism), RNA, Messenger (metabolism), RNA-Binding Proteins (genetics), RNA-Binding Proteins (metabolism), Real-Time Polymerase Chain Reaction (MeSH).
- MESH :
- chemical , genetics : RNA-Binding Proteins.
- chemical , metabolism : RNA, Messenger, RNA-Binding Proteins.
- chemical , toxicity : Environmental Pollutants, Lead.
- drug effects : Gene Expression Regulation, Phanerochaete.
- genetics : Phanerochaete.
- metabolism : Phanerochaete.
- Environmental Exposure, Environmental Monitoring, Real-Time Polymerase Chain Reaction.
Abstract
The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 μM lead, respectively. 50 and 100 μM lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the expression of ATP-dependent RNA helicase. Polyadenylate-binding protein mRNA levels did not reveal any significant increase when cells exposed to the concentrations of lead employed. However, the mRNA level of polyadenylate-binding protein and splicing factor RNPS1 within a period of 1 and 2 h temporal exposure to 100 μM lead showed 2.5 (p < 0.001) and 3.4-fold (p < 0.001) increase, respectively. Expression level of ATP-dependent RNA helicase was not affected from the period of exposure to this metal.
DOI: 10.1007/s00128-012-0886-2
PubMed: 23160747
Links toward previous steps (curation, corpus...)
- to stream Main, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000392
Links to Exploration step
pubmed:23160747Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Real-time PCR investigation of the dynamic expression of three "RNA processing and modification" genes of Phanerochaete chrysosporium exposed to lead.</title>
<author><name sortKey="Tekin, Elif" sort="Tekin, Elif" uniqKey="Tekin E" first="Elif" last="Tekin">Elif Tekin</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biological Sciences, Middle East Technical University, 06800, Ankara, Turkey.</nlm:affiliation>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>Department of Biological Sciences, Middle East Technical University, 06800, Ankara</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Icgen, Bulent" sort="Icgen, Bulent" uniqKey="Icgen B" first="Bulent" last="Icgen">Bulent Icgen</name>
</author>
<author><name sortKey="Ozcengiz, Gulay" sort="Ozcengiz, Gulay" uniqKey="Ozcengiz G" first="Gulay" last="Ozcengiz">Gulay Ozcengiz</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2013">2013</date>
<idno type="RBID">pubmed:23160747</idno>
<idno type="pmid">23160747</idno>
<idno type="doi">10.1007/s00128-012-0886-2</idno>
<idno type="wicri:Area/Main/Corpus">000392</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000392</idno>
<idno type="wicri:Area/Main/Curation">000392</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000392</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Real-time PCR investigation of the dynamic expression of three "RNA processing and modification" genes of Phanerochaete chrysosporium exposed to lead.</title>
<author><name sortKey="Tekin, Elif" sort="Tekin, Elif" uniqKey="Tekin E" first="Elif" last="Tekin">Elif Tekin</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biological Sciences, Middle East Technical University, 06800, Ankara, Turkey.</nlm:affiliation>
<country xml:lang="fr">Turquie</country>
<wicri:regionArea>Department of Biological Sciences, Middle East Technical University, 06800, Ankara</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Icgen, Bulent" sort="Icgen, Bulent" uniqKey="Icgen B" first="Bulent" last="Icgen">Bulent Icgen</name>
</author>
<author><name sortKey="Ozcengiz, Gulay" sort="Ozcengiz, Gulay" uniqKey="Ozcengiz G" first="Gulay" last="Ozcengiz">Gulay Ozcengiz</name>
</author>
</analytic>
<series><title level="j">Bulletin of environmental contamination and toxicology</title>
<idno type="eISSN">1432-0800</idno>
<imprint><date when="2013" type="published">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Environmental Exposure (MeSH)</term>
<term>Environmental Monitoring (MeSH)</term>
<term>Environmental Pollutants (toxicity)</term>
<term>Gene Expression Regulation (drug effects)</term>
<term>Lead (toxicity)</term>
<term>Phanerochaete (drug effects)</term>
<term>Phanerochaete (genetics)</term>
<term>Phanerochaete (metabolism)</term>
<term>RNA, Messenger (metabolism)</term>
<term>RNA-Binding Proteins (genetics)</term>
<term>RNA-Binding Proteins (metabolism)</term>
<term>Real-Time Polymerase Chain Reaction (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (métabolisme)</term>
<term>Exposition environnementale (MeSH)</term>
<term>Phanerochaete (effets des médicaments et des substances chimiques)</term>
<term>Phanerochaete (génétique)</term>
<term>Phanerochaete (métabolisme)</term>
<term>Plomb (toxicité)</term>
<term>Polluants environnementaux (toxicité)</term>
<term>Protéines de liaison à l'ARN (génétique)</term>
<term>Protéines de liaison à l'ARN (métabolisme)</term>
<term>Réaction de polymérisation en chaine en temps réel (MeSH)</term>
<term>Régulation de l'expression des gènes (effets des médicaments et des substances chimiques)</term>
<term>Surveillance de l'environnement (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA-Binding Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Messenger</term>
<term>RNA-Binding Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Environmental Pollutants</term>
<term>Lead</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation</term>
<term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Phanerochaete</term>
<term>Régulation de l'expression des gènes</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phanerochaete</term>
<term>Protéines de liaison à l'ARN</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term>
<term>Phanerochaete</term>
<term>Protéines de liaison à l'ARN</term>
</keywords>
<keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Plomb</term>
<term>Polluants environnementaux</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Environmental Exposure</term>
<term>Environmental Monitoring</term>
<term>Real-Time Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Exposition environnementale</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
<term>Surveillance de l'environnement</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 μM lead, respectively. 50 and 100 μM lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the expression of ATP-dependent RNA helicase. Polyadenylate-binding protein mRNA levels did not reveal any significant increase when cells exposed to the concentrations of lead employed. However, the mRNA level of polyadenylate-binding protein and splicing factor RNPS1 within a period of 1 and 2 h temporal exposure to 100 μM lead showed 2.5 (p < 0.001) and 3.4-fold (p < 0.001) increase, respectively. Expression level of ATP-dependent RNA helicase was not affected from the period of exposure to this metal.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23160747</PMID>
<DateCompleted><Year>2013</Year>
<Month>06</Month>
<Day>17</Day>
</DateCompleted>
<DateRevised><Year>2013</Year>
<Month>11</Month>
<Day>21</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0800</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>90</Volume>
<Issue>1</Issue>
<PubDate><Year>2013</Year>
<Month>Jan</Month>
</PubDate>
</JournalIssue>
<Title>Bulletin of environmental contamination and toxicology</Title>
<ISOAbbreviation>Bull Environ Contam Toxicol</ISOAbbreviation>
</Journal>
<ArticleTitle>Real-time PCR investigation of the dynamic expression of three "RNA processing and modification" genes of Phanerochaete chrysosporium exposed to lead.</ArticleTitle>
<Pagination><MedlinePgn>27-33</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1007/s00128-012-0886-2</ELocationID>
<Abstract><AbstractText>The expression of three ribosome binding proteins namely; polyadenylate-binding protein, splicing factor RNPS1 and ATP-dependent RNA helicase of Phanerochaete chrysosporium exposed to lead were analyzed by real-time PCR. The mRNA level of splicing factor RNPS1 showed 2.7 (p < 0.05), 2.6 (p < 0.05) and 4.9-fold (p < 0.001) increase when the cells were exposed to 25, 50 and 100 μM lead, respectively. 50 and 100 μM lead exposure resulted in almost 2-fold (p < 0.01and p < 0.05, respectively) increase in the expression of ATP-dependent RNA helicase. Polyadenylate-binding protein mRNA levels did not reveal any significant increase when cells exposed to the concentrations of lead employed. However, the mRNA level of polyadenylate-binding protein and splicing factor RNPS1 within a period of 1 and 2 h temporal exposure to 100 μM lead showed 2.5 (p < 0.001) and 3.4-fold (p < 0.001) increase, respectively. Expression level of ATP-dependent RNA helicase was not affected from the period of exposure to this metal.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tekin</LastName>
<ForeName>Elif</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Department of Biological Sciences, Middle East Technical University, 06800, Ankara, Turkey.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Icgen</LastName>
<ForeName>Bulent</ForeName>
<Initials>B</Initials>
</Author>
<Author ValidYN="Y"><LastName>Ozcengiz</LastName>
<ForeName>Gulay</ForeName>
<Initials>G</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2012</Year>
<Month>11</Month>
<Day>16</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Bull Environ Contam Toxicol</MedlineTA>
<NlmUniqueID>0046021</NlmUniqueID>
<ISSNLinking>0007-4861</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004785">Environmental Pollutants</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D016601">RNA-Binding Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>2P299V784P</RegistryNumber>
<NameOfSubstance UI="D007854">Lead</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D004781" MajorTopicYN="Y">Environmental Exposure</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004784" MajorTopicYN="N">Environmental Monitoring</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004785" MajorTopicYN="N">Environmental Pollutants</DescriptorName>
<QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007854" MajorTopicYN="N">Lead</DescriptorName>
<QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016601" MajorTopicYN="N">RNA-Binding Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year>
<Month>09</Month>
<Day>25</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2012</Year>
<Month>11</Month>
<Day>09</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2012</Year>
<Month>11</Month>
<Day>20</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2012</Year>
<Month>11</Month>
<Day>20</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2013</Year>
<Month>6</Month>
<Day>19</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">23160747</ArticleId>
<ArticleId IdType="doi">10.1007/s00128-012-0886-2</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000392 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 000392 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= PhanerochaeteV1 |flux= Main |étape= Curation |type= RBID |clé= pubmed:23160747 |texte= Real-time PCR investigation of the dynamic expression of three "RNA processing and modification" genes of Phanerochaete chrysosporium exposed to lead. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Curation/RBID.i -Sk "pubmed:23160747" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Curation/biblio.hfd \ | NlmPubMed2Wicri -a PhanerochaeteV1
![]() | This area was generated with Dilib version V0.6.37. | ![]() |