A comparison of ergosterol and PLFA methods for monitoring the growth of ligninolytic fungi during wheat straw solid state cultivation.
Identifieur interne : 000118 ( Main/Curation ); précédent : 000117; suivant : 000119A comparison of ergosterol and PLFA methods for monitoring the growth of ligninolytic fungi during wheat straw solid state cultivation.
Auteurs : Irnia Nurika [Indonésie] ; Daniel C. Eastwood [Royaume-Uni] ; Guy C. Barker [Royaume-Uni]Source :
- Journal of microbiological methods [ 1872-8359 ] ; 2018.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Acide linoléique, Ergostérol, Phospholipides.
- composition chimique : Champignons.
- croissance et développement : Champignons.
- microbiologie : Tiges de plante, Triticum.
- méthodes : Techniques microbiologiques.
- Phanerochaete, Schizophyllum.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Ergosterol, Linoleic Acid, Phospholipids.
- chemistry : Fungi.
- growth & development : Fungi.
- methods : Microbiological Techniques.
- microbiology : Plant Stems, Triticum.
- Phanerochaete, Schizophyllum.
Abstract
Ergosterol, total phospholipid fatty acid (PLFA) and linoleic acid (18:2n-6) have all been used to determine fungal growth. This paper compares these methods to assess the growth of four different saprotrophic fungal species during solid state cultivation using a wheat straw substrate that have not been compared or measured previously. Ergosterol production appeared to track the mycelia growth well but its production differed considerably between fungi. This means that a specific conversion factor needs to be determined and applied for any given fungus. In comparison, measurements of total PLFA and linoleic acid only showed promise for determining the growth of Postia placenta due to the positive correlation with ergosterol measurements. In contrast, the other fungi tested (Phanerochaete chrysosporium, Serpula lacrymans and Schizophyllum commune) showed either no correlation or in some cases a negative correlation using this assay. The novel findings highlight the variation in fungal fatty acid between species, culture conditions and durations of incubation; suggesting that measurement of linoleic acid is only usable in specific cases. These findings provide important consideration for the study of fungi growing in solid substrates and suggest that the use of PLFA might bias diversity indices.
DOI: 10.1016/j.mimet.2018.03.006
PubMed: 29580983
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Eastwood</name><affiliation wicri:level="1"><nlm:affiliation>Department Biosciences, Swansea University, Swansea SA2 8PP, United Kingdom. Electronic address: Eastwood@swansea.ac.uk.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department Biosciences, Swansea University, Swansea SA2 8PP</wicri:regionArea></affiliation></author><author><name sortKey="Barker, Guy C" sort="Barker, Guy C" uniqKey="Barker G" first="Guy C" last="Barker">Guy C. Barker</name><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom. 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Electronic address: irnia@ub.ac.id.</nlm:affiliation><country xml:lang="fr">Indonésie</country><wicri:regionArea>Department Agroindustrial Technology, Faculty of Agricultural Technology, Universitas Brawijaya, Malang 65145</wicri:regionArea></affiliation></author><author><name sortKey="Eastwood, Daniel C" sort="Eastwood, Daniel C" uniqKey="Eastwood D" first="Daniel C" last="Eastwood">Daniel C. Eastwood</name><affiliation wicri:level="1"><nlm:affiliation>Department Biosciences, Swansea University, Swansea SA2 8PP, United Kingdom. Electronic address: Eastwood@swansea.ac.uk.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department Biosciences, Swansea University, Swansea SA2 8PP</wicri:regionArea></affiliation></author><author><name sortKey="Barker, Guy C" sort="Barker, Guy C" uniqKey="Barker G" first="Guy C" last="Barker">Guy C. Barker</name><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom. 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This paper compares these methods to assess the growth of four different saprotrophic fungal species during solid state cultivation using a wheat straw substrate that have not been compared or measured previously. Ergosterol production appeared to track the mycelia growth well but its production differed considerably between fungi. This means that a specific conversion factor needs to be determined and applied for any given fungus. In comparison, measurements of total PLFA and linoleic acid only showed promise for determining the growth of Postia placenta due to the positive correlation with ergosterol measurements. In contrast, the other fungi tested (Phanerochaete chrysosporium, Serpula lacrymans and Schizophyllum commune) showed either no correlation or in some cases a negative correlation using this assay. The novel findings highlight the variation in fungal fatty acid between species, culture conditions and durations of incubation; suggesting that measurement of linoleic acid is only usable in specific cases. These findings provide important consideration for the study of fungi growing in solid substrates and suggest that the use of PLFA might bias diversity indices.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29580983</PMID><DateCompleted><Year>2019</Year><Month>04</Month><Day>12</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-8359</ISSN><JournalIssue CitedMedium="Internet"><Volume>148</Volume><PubDate><Year>2018</Year><Month>05</Month></PubDate></JournalIssue><Title>Journal of microbiological methods</Title><ISOAbbreviation>J Microbiol Methods</ISOAbbreviation></Journal><ArticleTitle>A comparison of ergosterol and PLFA methods for monitoring the growth of ligninolytic fungi during wheat straw solid state cultivation.</ArticleTitle><Pagination><MedlinePgn>49-54</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0167-7012(18)30196-9</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.mimet.2018.03.006</ELocationID><Abstract><AbstractText>Ergosterol, total phospholipid fatty acid (PLFA) and linoleic acid (18:2n-6) have all been used to determine fungal growth. This paper compares these methods to assess the growth of four different saprotrophic fungal species during solid state cultivation using a wheat straw substrate that have not been compared or measured previously. Ergosterol production appeared to track the mycelia growth well but its production differed considerably between fungi. This means that a specific conversion factor needs to be determined and applied for any given fungus. In comparison, measurements of total PLFA and linoleic acid only showed promise for determining the growth of Postia placenta due to the positive correlation with ergosterol measurements. In contrast, the other fungi tested (Phanerochaete chrysosporium, Serpula lacrymans and Schizophyllum commune) showed either no correlation or in some cases a negative correlation using this assay. The novel findings highlight the variation in fungal fatty acid between species, culture conditions and durations of incubation; suggesting that measurement of linoleic acid is only usable in specific cases. These findings provide important consideration for the study of fungi growing in solid substrates and suggest that the use of PLFA might bias diversity indices.</AbstractText><CopyrightInformation>Copyright © 2018. Published by Elsevier B.V.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nurika</LastName><ForeName>Irnia</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department Agroindustrial Technology, Faculty of Agricultural Technology, Universitas Brawijaya, Malang 65145, Indonesia. Electronic address: irnia@ub.ac.id.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eastwood</LastName><ForeName>Daniel C</ForeName><Initials>DC</Initials><AffiliationInfo><Affiliation>Department Biosciences, Swansea University, Swansea SA2 8PP, United Kingdom. Electronic address: Eastwood@swansea.ac.uk.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barker</LastName><ForeName>Guy C</ForeName><Initials>GC</Initials><AffiliationInfo><Affiliation>School of Life Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom. Electronic address: guy.barker@warwick.ac.uk.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>03</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Microbiol Methods</MedlineTA><NlmUniqueID>8306883</NlmUniqueID><ISSNLinking>0167-7012</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010743">Phospholipids</NameOfSubstance></Chemical><Chemical><RegistryNumber>9KJL21T0QJ</RegistryNumber><NameOfSubstance UI="D019787">Linoleic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>Z30RAY509F</RegistryNumber><NameOfSubstance UI="D004875">Ergosterol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004875" MajorTopicYN="N">Ergosterol</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019787" MajorTopicYN="N">Linoleic Acid</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008828" MajorTopicYN="N">Microbiological Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010743" MajorTopicYN="N">Phospholipids</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018547" MajorTopicYN="N">Plant Stems</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012567" MajorTopicYN="N">Schizophyllum</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Ergosterol</Keyword><Keyword MajorTopicYN="Y">Fungal biomass</Keyword><Keyword MajorTopicYN="Y">Linoleic acid</Keyword><Keyword MajorTopicYN="Y">Phospholipid fatty acid</Keyword><Keyword MajorTopicYN="Y">Solid state cultivation</Keyword><Keyword MajorTopicYN="Y">Wheat straw</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>12</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>03</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>03</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>3</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>4</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>3</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29580983</ArticleId><ArticleId IdType="pii">S0167-7012(18)30196-9</ArticleId><ArticleId IdType="doi">10.1016/j.mimet.2018.03.006</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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