Physical and enzymatic properties of a new manganese peroxidase from the white-rot fungus Trametes pubescens strain i8 for lignin biodegradation and textile-dyes biodecolorization.
Identifieur interne : 000087 ( Main/Curation ); précédent : 000086; suivant : 000088Physical and enzymatic properties of a new manganese peroxidase from the white-rot fungus Trametes pubescens strain i8 for lignin biodegradation and textile-dyes biodecolorization.
Auteurs : Hatem Rekik [Tunisie] ; Nadia Zaraî Jaouadi [Tunisie] ; Khelifa Bouacem [Algérie] ; Bilal Zenati [Algérie] ; Sidali Kourdali [Algérie] ; Abdelmalek Badis [Algérie] ; Rachid Annane [Algérie] ; Amel Bouanane-Darenfed [Algérie] ; Samir Bejar [Tunisie] ; Bassem Jaouadi [Tunisie]Source :
- International journal of biological macromolecules [ 1879-0003 ] ; 2019.
Descripteurs français
- KwdFr :
- Acides aminés (métabolisme), Agents colorants (métabolisme), Algérie (MeSH), Catalyse (MeSH), Champignons (enzymologie), Champignons (métabolisme), Coriolaceae (enzymologie), Coriolaceae (métabolisme), Dépollution biologique de l'environnement (MeSH), Horseradish peroxidase (métabolisme), Lignine (métabolisme), Peroxidases (métabolisme), Phanerochaete (métabolisme), Textiles (MeSH), Trametes (métabolisme).
- MESH :
- enzymologie : Champignons, Coriolaceae.
- métabolisme : Acides aminés, Agents colorants, Champignons, Coriolaceae, Horseradish peroxidase, Lignine, Peroxidases, Phanerochaete, Trametes.
- Algérie, Catalyse, Dépollution biologique de l'environnement, Textiles.
- Wicri :
- geographic : Algérie.
English descriptors
- KwdEn :
- Algeria (MeSH), Amino Acids (metabolism), Biodegradation, Environmental (MeSH), Catalysis (MeSH), Coloring Agents (metabolism), Coriolaceae (enzymology), Coriolaceae (metabolism), Fungi (enzymology), Fungi (metabolism), Horseradish Peroxidase (metabolism), Lignin (metabolism), Peroxidases (metabolism), Phanerochaete (metabolism), Textiles (MeSH), Trametes (metabolism).
- MESH :
- chemical , metabolism : Amino Acids, Coloring Agents, Horseradish Peroxidase, Lignin, Peroxidases.
- geographic : Algeria.
- enzymology : Coriolaceae, Fungi.
- metabolism : Coriolaceae, Fungi, Phanerochaete, Trametes.
- Biodegradation, Environmental, Catalysis, Textiles.
Abstract
A new manganese peroxidase-producing white-rot basidiomycete fungus was isolated from symptomatic wood of the camphor trees Cinnamomum camphora (L.) at the Hamma Botanical Garden (Algeria) and identified as Trametes pubescens strain i8. The enzyme was purified (MnP TP55) to apparent electrophoretic homogeneity and biochemically characterized. The specific activity and Reinheitzahl value of the purified enzyme were 221 U/mg and 2.25, respectively. MALDI-TOF/MS analysis revealed that the purified enzyme was a monomer with a molecular mass of 55.2 kDa. The NH2-terminal sequence of the first 26 amino acid residues of MnP TP55 showed high similarity with those of white-rot fungal peroxidases. It revealed optimal activity at pH 5 and 40 °C. This peroxidase was completely inhibited by sodium azide and potassium cyanide, suggesting the presence of heme-components in its tertiary structure. Interestingly, MnP TP55 showed higher catalytic efficiency, organic solvent-tolerance, dye-decolorization ability, and detergent-compatibility than that of horseradish peroxidase (HRP) from roots of Armoracia rustanica, manganese peroxidase from Bjerkandera adusta strain CX-9 (MnP BA30), and manganese peroxidase from Phanerochaete chrysosporium (MnP PC). Overall, the findings provide strong support for the potential candidacy of MnP TP55 for environmental applications, mainly the development of enzyme-based technologies for lignin biodegradation, textile-dyes biodecolorization, and detergent formulations.
DOI: 10.1016/j.ijbiomac.2018.12.053
PubMed: 30528991
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Physical and enzymatic properties of a new manganese peroxidase from the white-rot fungus Trametes pubescens strain i8 for lignin biodegradation and textile-dyes biodecolorization.</title><author><name sortKey="Rekik, Hatem" sort="Rekik, Hatem" uniqKey="Rekik H" first="Hatem" last="Rekik">Hatem Rekik</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018</wicri:regionArea></affiliation></author><author><name sortKey="Zarai Jaouadi, Nadia" sort="Zarai Jaouadi, Nadia" uniqKey="Zarai Jaouadi N" first="Nadia" last="Zaraî Jaouadi">Nadia Zaraî Jaouadi</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018</wicri:regionArea></affiliation></author><author><name sortKey="Bouacem, Khelifa" sort="Bouacem, Khelifa" uniqKey="Bouacem K" first="Khelifa" last="Bouacem">Khelifa Bouacem</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers</wicri:regionArea></affiliation></author><author><name sortKey="Zenati, Bilal" sort="Zenati, Bilal" uniqKey="Zenati B" first="Bilal" last="Zenati">Bilal Zenati</name><affiliation wicri:level="1"><nlm:affiliation>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza</wicri:regionArea></affiliation></author><author><name sortKey="Kourdali, Sidali" sort="Kourdali, Sidali" uniqKey="Kourdali S" first="Sidali" last="Kourdali">Sidali Kourdali</name><affiliation wicri:level="1"><nlm:affiliation>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza</wicri:regionArea></affiliation></author><author><name sortKey="Badis, Abdelmalek" sort="Badis, Abdelmalek" uniqKey="Badis A" first="Abdelmalek" last="Badis">Abdelmalek Badis</name><affiliation wicri:level="1"><nlm:affiliation>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria; Laboratory of Natural Products Chemistry and Biomolecules (LNPC-BioM), Faculty of Sciences, University of Blida 1, Road of Soumaâ, PO Box 270, 09000 Blida, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria; Laboratory of Natural Products Chemistry and Biomolecules (LNPC-BioM), Faculty of Sciences, University of Blida 1, Road of Soumaâ, PO Box 270, 09000 Blida</wicri:regionArea></affiliation></author><author><name sortKey="Annane, Rachid" sort="Annane, Rachid" uniqKey="Annane R" first="Rachid" last="Annane">Rachid Annane</name><affiliation wicri:level="1"><nlm:affiliation>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza</wicri:regionArea></affiliation></author><author><name sortKey="Bouanane Darenfed, Amel" sort="Bouanane Darenfed, Amel" uniqKey="Bouanane Darenfed A" first="Amel" last="Bouanane-Darenfed">Amel Bouanane-Darenfed</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers</wicri:regionArea></affiliation></author><author><name sortKey="Bejar, Samir" sort="Bejar, Samir" uniqKey="Bejar S" first="Samir" last="Bejar">Samir Bejar</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018</wicri:regionArea></affiliation></author><author><name sortKey="Jaouadi, Bassem" sort="Jaouadi, Bassem" uniqKey="Jaouadi B" first="Bassem" last="Jaouadi">Bassem Jaouadi</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia. Electronic address: bassem.jaouadi@cbs.rnrt.tn.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30528991</idno><idno type="pmid">30528991</idno><idno type="doi">10.1016/j.ijbiomac.2018.12.053</idno><idno type="wicri:Area/Main/Corpus">000087</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000087</idno><idno type="wicri:Area/Main/Curation">000087</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000087</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Physical and enzymatic properties of a new manganese peroxidase from the white-rot fungus Trametes pubescens strain i8 for lignin biodegradation and textile-dyes biodecolorization.</title><author><name sortKey="Rekik, Hatem" sort="Rekik, Hatem" uniqKey="Rekik H" first="Hatem" last="Rekik">Hatem Rekik</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018</wicri:regionArea></affiliation></author><author><name sortKey="Zarai Jaouadi, Nadia" sort="Zarai Jaouadi, Nadia" uniqKey="Zarai Jaouadi N" first="Nadia" last="Zaraî Jaouadi">Nadia Zaraî Jaouadi</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018</wicri:regionArea></affiliation></author><author><name sortKey="Bouacem, Khelifa" sort="Bouacem, Khelifa" uniqKey="Bouacem K" first="Khelifa" last="Bouacem">Khelifa Bouacem</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers</wicri:regionArea></affiliation></author><author><name sortKey="Zenati, Bilal" sort="Zenati, Bilal" uniqKey="Zenati B" first="Bilal" last="Zenati">Bilal Zenati</name><affiliation wicri:level="1"><nlm:affiliation>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza</wicri:regionArea></affiliation></author><author><name sortKey="Kourdali, Sidali" sort="Kourdali, Sidali" uniqKey="Kourdali S" first="Sidali" last="Kourdali">Sidali Kourdali</name><affiliation wicri:level="1"><nlm:affiliation>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza</wicri:regionArea></affiliation></author><author><name sortKey="Badis, Abdelmalek" sort="Badis, Abdelmalek" uniqKey="Badis A" first="Abdelmalek" last="Badis">Abdelmalek Badis</name><affiliation wicri:level="1"><nlm:affiliation>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria; Laboratory of Natural Products Chemistry and Biomolecules (LNPC-BioM), Faculty of Sciences, University of Blida 1, Road of Soumaâ, PO Box 270, 09000 Blida, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria; Laboratory of Natural Products Chemistry and Biomolecules (LNPC-BioM), Faculty of Sciences, University of Blida 1, Road of Soumaâ, PO Box 270, 09000 Blida</wicri:regionArea></affiliation></author><author><name sortKey="Annane, Rachid" sort="Annane, Rachid" uniqKey="Annane R" first="Rachid" last="Annane">Rachid Annane</name><affiliation wicri:level="1"><nlm:affiliation>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza</wicri:regionArea></affiliation></author><author><name sortKey="Bouanane Darenfed, Amel" sort="Bouanane Darenfed, Amel" uniqKey="Bouanane Darenfed A" first="Amel" last="Bouanane-Darenfed">Amel Bouanane-Darenfed</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.</nlm:affiliation><country xml:lang="fr">Algérie</country><wicri:regionArea>Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers</wicri:regionArea></affiliation></author><author><name sortKey="Bejar, Samir" sort="Bejar, Samir" uniqKey="Bejar S" first="Samir" last="Bejar">Samir Bejar</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018</wicri:regionArea></affiliation></author><author><name sortKey="Jaouadi, Bassem" sort="Jaouadi, Bassem" uniqKey="Jaouadi B" first="Bassem" last="Jaouadi">Bassem Jaouadi</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia. Electronic address: bassem.jaouadi@cbs.rnrt.tn.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018</wicri:regionArea></affiliation></author></analytic><series><title level="j">International journal of biological macromolecules</title><idno type="eISSN">1879-0003</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algeria (MeSH)</term><term>Amino Acids (metabolism)</term><term>Biodegradation, Environmental (MeSH)</term><term>Catalysis (MeSH)</term><term>Coloring Agents (metabolism)</term><term>Coriolaceae (enzymology)</term><term>Coriolaceae (metabolism)</term><term>Fungi (enzymology)</term><term>Fungi (metabolism)</term><term>Horseradish Peroxidase (metabolism)</term><term>Lignin (metabolism)</term><term>Peroxidases (metabolism)</term><term>Phanerochaete (metabolism)</term><term>Textiles (MeSH)</term><term>Trametes (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides aminés (métabolisme)</term><term>Agents colorants (métabolisme)</term><term>Algérie (MeSH)</term><term>Catalyse (MeSH)</term><term>Champignons (enzymologie)</term><term>Champignons (métabolisme)</term><term>Coriolaceae (enzymologie)</term><term>Coriolaceae (métabolisme)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Horseradish peroxidase (métabolisme)</term><term>Lignine (métabolisme)</term><term>Peroxidases (métabolisme)</term><term>Phanerochaete (métabolisme)</term><term>Textiles (MeSH)</term><term>Trametes (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amino Acids</term><term>Coloring Agents</term><term>Horseradish Peroxidase</term><term>Lignin</term><term>Peroxidases</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Algeria</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Champignons</term><term>Coriolaceae</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Coriolaceae</term><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Coriolaceae</term><term>Fungi</term><term>Phanerochaete</term><term>Trametes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides aminés</term><term>Agents colorants</term><term>Champignons</term><term>Coriolaceae</term><term>Horseradish peroxidase</term><term>Lignine</term><term>Peroxidases</term><term>Phanerochaete</term><term>Trametes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Catalysis</term><term>Textiles</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Algérie</term><term>Catalyse</term><term>Dépollution biologique de l'environnement</term><term>Textiles</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Algérie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new manganese peroxidase-producing white-rot basidiomycete fungus was isolated from symptomatic wood of the camphor trees Cinnamomum camphora (L.) at the Hamma Botanical Garden (Algeria) and identified as Trametes pubescens strain i8. The enzyme was purified (MnP TP55) to apparent electrophoretic homogeneity and biochemically characterized. The specific activity and Reinheitzahl value of the purified enzyme were 221 U/mg and 2.25, respectively. MALDI-TOF/MS analysis revealed that the purified enzyme was a monomer with a molecular mass of 55.2 kDa. The NH<sub>2</sub>-terminal sequence of the first 26 amino acid residues of MnP TP55 showed high similarity with those of white-rot fungal peroxidases. It revealed optimal activity at pH 5 and 40 °C. This peroxidase was completely inhibited by sodium azide and potassium cyanide, suggesting the presence of heme-components in its tertiary structure. Interestingly, MnP TP55 showed higher catalytic efficiency, organic solvent-tolerance, dye-decolorization ability, and detergent-compatibility than that of horseradish peroxidase (HRP) from roots of Armoracia rustanica, manganese peroxidase from Bjerkandera adusta strain CX-9 (MnP BA30), and manganese peroxidase from Phanerochaete chrysosporium (MnP PC). Overall, the findings provide strong support for the potential candidacy of MnP TP55 for environmental applications, mainly the development of enzyme-based technologies for lignin biodegradation, textile-dyes biodecolorization, and detergent formulations.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30528991</PMID><DateCompleted><Year>2019</Year><Month>05</Month><Day>16</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0003</ISSN><JournalIssue CitedMedium="Internet"><Volume>125</Volume><PubDate><Year>2019</Year><Month>Mar</Month><Day>15</Day></PubDate></JournalIssue><Title>International journal of biological macromolecules</Title><ISOAbbreviation>Int J Biol Macromol</ISOAbbreviation></Journal><ArticleTitle>Physical and enzymatic properties of a new manganese peroxidase from the white-rot fungus Trametes pubescens strain i8 for lignin biodegradation and textile-dyes biodecolorization.</ArticleTitle><Pagination><MedlinePgn>514-525</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0141-8130(18)33140-4</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ijbiomac.2018.12.053</ELocationID><Abstract><AbstractText>A new manganese peroxidase-producing white-rot basidiomycete fungus was isolated from symptomatic wood of the camphor trees Cinnamomum camphora (L.) at the Hamma Botanical Garden (Algeria) and identified as Trametes pubescens strain i8. The enzyme was purified (MnP TP55) to apparent electrophoretic homogeneity and biochemically characterized. The specific activity and Reinheitzahl value of the purified enzyme were 221 U/mg and 2.25, respectively. MALDI-TOF/MS analysis revealed that the purified enzyme was a monomer with a molecular mass of 55.2 kDa. The NH<sub>2</sub>-terminal sequence of the first 26 amino acid residues of MnP TP55 showed high similarity with those of white-rot fungal peroxidases. It revealed optimal activity at pH 5 and 40 °C. This peroxidase was completely inhibited by sodium azide and potassium cyanide, suggesting the presence of heme-components in its tertiary structure. Interestingly, MnP TP55 showed higher catalytic efficiency, organic solvent-tolerance, dye-decolorization ability, and detergent-compatibility than that of horseradish peroxidase (HRP) from roots of Armoracia rustanica, manganese peroxidase from Bjerkandera adusta strain CX-9 (MnP BA30), and manganese peroxidase from Phanerochaete chrysosporium (MnP PC). Overall, the findings provide strong support for the potential candidacy of MnP TP55 for environmental applications, mainly the development of enzyme-based technologies for lignin biodegradation, textile-dyes biodecolorization, and detergent formulations.</AbstractText><CopyrightInformation>Copyright © 2018. Published by Elsevier B.V.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rekik</LastName><ForeName>Hatem</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zaraî Jaouadi</LastName><ForeName>Nadia</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bouacem</LastName><ForeName>Khelifa</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zenati</LastName><ForeName>Bilal</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kourdali</LastName><ForeName>Sidali</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Badis</LastName><ForeName>Abdelmalek</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria; Laboratory of Natural Products Chemistry and Biomolecules (LNPC-BioM), Faculty of Sciences, University of Blida 1, Road of Soumaâ, PO Box 270, 09000 Blida, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Annane</LastName><ForeName>Rachid</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bouanane-Darenfed</LastName><ForeName>Amel</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratory of Cellular and Molecular Biology, Microbiology Team, Faculty of Biological Sciences, University of Sciences and Technology of Houari Boumediene (USTHB), PO Box 32, El Alia, Bab Ezzouar, 16111, Algiers, Algeria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bejar</LastName><ForeName>Samir</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jaouadi</LastName><ForeName>Bassem</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia. Electronic address: bassem.jaouadi@cbs.rnrt.tn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>12</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Int J Biol Macromol</MedlineTA><NlmUniqueID>7909578</NlmUniqueID><ISSNLinking>0141-8130</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000596">Amino Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004396">Coloring Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D006735">Horseradish Peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000462" MajorTopicYN="N" Type="Geographic">Algeria</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000596" MajorTopicYN="N">Amino Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002384" MajorTopicYN="N">Catalysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004396" MajorTopicYN="N">Coloring Agents</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055453" MajorTopicYN="N">Coriolaceae</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006735" MajorTopicYN="N">Horseradish Peroxidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013784" MajorTopicYN="N">Textiles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055454" MajorTopicYN="N">Trametes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Detergent-compatibility</Keyword><Keyword MajorTopicYN="N">Lignin biodegradation and textile-dyes biodecolorization</Keyword><Keyword MajorTopicYN="N">Manganese peroxidase</Keyword><Keyword MajorTopicYN="N">Organic solvent-tolerance</Keyword><Keyword MajorTopicYN="N">White-rot fungi</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>06</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>12</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>12</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>12</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>5</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>12</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30528991</ArticleId><ArticleId IdType="pii">S0141-8130(18)33140-4</ArticleId><ArticleId IdType="doi">10.1016/j.ijbiomac.2018.12.053</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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