Release of mutagens after chemical or microbial degradation of beech wood lignin.
Identifieur interne : 000F64 ( Main/Corpus ); précédent : 000F63; suivant : 000F65Release of mutagens after chemical or microbial degradation of beech wood lignin.
Auteurs : E. Mohtashamipur ; K. NorpothSource :
- Toxicology letters [ 0378-4274 ] ; 1990.
English descriptors
- KwdEn :
- MESH :
- chemical , isolation & purification : Mutagens.
- chemical , metabolism : Lignin.
- chemical : Dust.
- metabolism : Fungi.
- Animals, Biodegradation, Environmental, Mutagenicity Tests, Rats, Wood.
Abstract
The microbial or chemical degradation of lignin from untreated samples of beech wood dusts (Fagus silvatica) resulted in the release of different mutagenic responses in the Salmonella/mammalian plate incorporation assay. In the first experiment using chemical degradation of lignin, dust samples were pre-extracted using acetone-water; the lignin portions were degraded into simpler compounds which were further fractionated on a Sephadex-LH20 column. The compounds isolated from the second phase of Sephadex, representing substances with a 3-4 ring structure and/or those of the same molecular weight, were highly mutagenic towards Salmonella typhimurium TA100 in the presence of metabolic activation. These substances were also active to some extent in strain TA1537 both in the presence and absence of Aroclor-induced rat liver homogenates. In contrast, no direct- or indirect-acting mutagenicity was found when testing with strains TA97 and TA98. Strain TA1535 responded positively only to direct-acting mutagens in the fraction tested. The mutagenic fraction was found to be toxic to the cells when tested in a histidine-rich medium. Repurification of this mutagenic fraction, using silica-gel column chromatography, revealed much higher mutagenic activity than the test material towards strain TA100. In the second pilot experiment, Phanerochaete chrysosporium and Chaetomium globosum, which are known for their ability to degrade lignin, were each incubated with wood dusts in a mixture of physiological saline and nutrient broth for either 3 or 30 days. Significant mutagenic activity was observed with the dust extract after incubation with Ph. chrysosporium but not with Ch. globosum which is a known degrader of beech lignin. These results are discussed regarding hypotheses on the carcinogenicity of beech wood dusts.
DOI: 10.1016/0378-4274(90)90070-3
PubMed: 2339441
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pubmed:2339441Le document en format XML
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Norpoth</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1990">1990</date><idno type="RBID">pubmed:2339441</idno><idno type="pmid">2339441</idno><idno type="doi">10.1016/0378-4274(90)90070-3</idno><idno type="wicri:Area/Main/Corpus">000F64</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000F64</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Release of mutagens after chemical or microbial degradation of beech wood lignin.</title><author><name sortKey="Mohtashamipur, E" sort="Mohtashamipur, E" uniqKey="Mohtashamipur E" first="E" last="Mohtashamipur">E. Mohtashamipur</name><affiliation><nlm:affiliation>Institute of Hygiene and Occupational Medicine, University Medical Center, Essen University, F.R.G.</nlm:affiliation></affiliation></author><author><name sortKey="Norpoth, K" sort="Norpoth, K" uniqKey="Norpoth K" first="K" last="Norpoth">K. Norpoth</name></author></analytic><series><title level="j">Toxicology letters</title><idno type="ISSN">0378-4274</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Biodegradation, Environmental (MeSH)</term><term>Dust (MeSH)</term><term>Fungi (metabolism)</term><term>Lignin (metabolism)</term><term>Mutagenicity Tests (MeSH)</term><term>Mutagens (isolation & purification)</term><term>Rats (MeSH)</term><term>Wood (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Mutagens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Dust</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Biodegradation, Environmental</term><term>Mutagenicity Tests</term><term>Rats</term><term>Wood</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The microbial or chemical degradation of lignin from untreated samples of beech wood dusts (Fagus silvatica) resulted in the release of different mutagenic responses in the Salmonella/mammalian plate incorporation assay. In the first experiment using chemical degradation of lignin, dust samples were pre-extracted using acetone-water; the lignin portions were degraded into simpler compounds which were further fractionated on a Sephadex-LH20 column. The compounds isolated from the second phase of Sephadex, representing substances with a 3-4 ring structure and/or those of the same molecular weight, were highly mutagenic towards Salmonella typhimurium TA100 in the presence of metabolic activation. These substances were also active to some extent in strain TA1537 both in the presence and absence of Aroclor-induced rat liver homogenates. In contrast, no direct- or indirect-acting mutagenicity was found when testing with strains TA97 and TA98. Strain TA1535 responded positively only to direct-acting mutagens in the fraction tested. The mutagenic fraction was found to be toxic to the cells when tested in a histidine-rich medium. Repurification of this mutagenic fraction, using silica-gel column chromatography, revealed much higher mutagenic activity than the test material towards strain TA100. In the second pilot experiment, Phanerochaete chrysosporium and Chaetomium globosum, which are known for their ability to degrade lignin, were each incubated with wood dusts in a mixture of physiological saline and nutrient broth for either 3 or 30 days. Significant mutagenic activity was observed with the dust extract after incubation with Ph. chrysosporium but not with Ch. globosum which is a known degrader of beech lignin. These results are discussed regarding hypotheses on the carcinogenicity of beech wood dusts.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">2339441</PMID><DateCompleted><Year>1990</Year><Month>06</Month><Day>19</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0378-4274</ISSN><JournalIssue CitedMedium="Print"><Volume>51</Volume><Issue>3</Issue><PubDate><Year>1990</Year><Month>May</Month></PubDate></JournalIssue><Title>Toxicology letters</Title><ISOAbbreviation>Toxicol Lett</ISOAbbreviation></Journal><ArticleTitle>Release of mutagens after chemical or microbial degradation of beech wood lignin.</ArticleTitle><Pagination><MedlinePgn>277-85</MedlinePgn></Pagination><Abstract><AbstractText>The microbial or chemical degradation of lignin from untreated samples of beech wood dusts (Fagus silvatica) resulted in the release of different mutagenic responses in the Salmonella/mammalian plate incorporation assay. In the first experiment using chemical degradation of lignin, dust samples were pre-extracted using acetone-water; the lignin portions were degraded into simpler compounds which were further fractionated on a Sephadex-LH20 column. The compounds isolated from the second phase of Sephadex, representing substances with a 3-4 ring structure and/or those of the same molecular weight, were highly mutagenic towards Salmonella typhimurium TA100 in the presence of metabolic activation. These substances were also active to some extent in strain TA1537 both in the presence and absence of Aroclor-induced rat liver homogenates. In contrast, no direct- or indirect-acting mutagenicity was found when testing with strains TA97 and TA98. Strain TA1535 responded positively only to direct-acting mutagens in the fraction tested. The mutagenic fraction was found to be toxic to the cells when tested in a histidine-rich medium. Repurification of this mutagenic fraction, using silica-gel column chromatography, revealed much higher mutagenic activity than the test material towards strain TA100. In the second pilot experiment, Phanerochaete chrysosporium and Chaetomium globosum, which are known for their ability to degrade lignin, were each incubated with wood dusts in a mixture of physiological saline and nutrient broth for either 3 or 30 days. Significant mutagenic activity was observed with the dust extract after incubation with Ph. chrysosporium but not with Ch. globosum which is a known degrader of beech lignin. These results are discussed regarding hypotheses on the carcinogenicity of beech wood dusts.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mohtashamipur</LastName><ForeName>E</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Institute of Hygiene and Occupational Medicine, University Medical Center, Essen University, F.R.G.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Norpoth</LastName><ForeName>K</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Toxicol Lett</MedlineTA><NlmUniqueID>7709027</NlmUniqueID><ISSNLinking>0378-4274</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004391">Dust</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009153">Mutagens</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004391" MajorTopicYN="Y">Dust</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009152" MajorTopicYN="N">Mutagenicity Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009153" MajorTopicYN="N">Mutagens</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="Y">Wood</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1990</Year><Month>5</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1990</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1990</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">2339441</ArticleId><ArticleId IdType="pii">0378-4274(90)90070-3</ArticleId><ArticleId IdType="doi">10.1016/0378-4274(90)90070-3</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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