An improved transformation vector for the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium.
Identifieur interne : 000F14 ( Main/Corpus ); précédent : 000F13; suivant : 000F15An improved transformation vector for the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium.
Auteurs : T. Randall ; C A ReddySource :
- Gene [ 0378-1119 ] ; 1991.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Peroxidases.
- chemical , metabolism : Lignin.
- genetics : Basidiomycota, Escherichia coli, Genes, Fungal, Genetic Vectors, Kanamycin Resistance, Plasmids, Transformation, Genetic.
- metabolism : Basidiomycota.
- Blotting, Southern.
Abstract
In this study, a lignin peroxidase-encoding gene (LIP) of Phanerochaete chrysosporium was disrupted by inserting into its coding region the kanamycin-resistance determinant from Tn903. The resulting recombinant plasmid, pUGLG1: kan, was transformed into P. chrysosporium with the expectation that the disrupted gene might replace the homologous LIP gene in the chromosome. However, the results showed that pUGLG1: kan sequences do not integrate into the chromosome; instead, the plasmid is maintained intact in the transformants in an extrachromosomal state. Our data also show that pUGLG1: kan undergoes replication in P. chrysosporium, is maintained as a circular element, is recoverable from meiotic and mitotic progeny, although at a low frequency, and can be recovered intact by Escherichia coli transformation. These results suggest that the GLG1 component of pUGLG1: kan contains as yet unidentified sequences that allow its autonomous replication in P. chrysosporium transformants.
DOI: 10.1016/0378-1119(91)90403-x
PubMed: 1879693
Links to Exploration step
pubmed:1879693Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An improved transformation vector for the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium.</title>
<author><name sortKey="Randall, T" sort="Randall, T" uniqKey="Randall T" first="T" last="Randall">T. Randall</name>
<affiliation><nlm:affiliation>Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name>
</author>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">An improved transformation vector for the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium.</title>
<author><name sortKey="Randall, T" sort="Randall, T" uniqKey="Randall T" first="T" last="Randall">T. Randall</name>
<affiliation><nlm:affiliation>Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Reddy, C A" sort="Reddy, C A" uniqKey="Reddy C" first="C A" last="Reddy">C A Reddy</name>
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<series><title level="j">Gene</title>
<idno type="ISSN">0378-1119</idno>
<imprint><date when="1991" type="published">1991</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term>
<term>Basidiomycota (metabolism)</term>
<term>Blotting, Southern (MeSH)</term>
<term>Escherichia coli (genetics)</term>
<term>Genes, Fungal (genetics)</term>
<term>Genetic Vectors (genetics)</term>
<term>Kanamycin Resistance (genetics)</term>
<term>Lignin (metabolism)</term>
<term>Peroxidases (genetics)</term>
<term>Plasmids (genetics)</term>
<term>Transformation, Genetic (genetics)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peroxidases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
<term>Escherichia coli</term>
<term>Genes, Fungal</term>
<term>Genetic Vectors</term>
<term>Kanamycin Resistance</term>
<term>Plasmids</term>
<term>Transformation, Genetic</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Blotting, Southern</term>
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<front><div type="abstract" xml:lang="en">In this study, a lignin peroxidase-encoding gene (LIP) of Phanerochaete chrysosporium was disrupted by inserting into its coding region the kanamycin-resistance determinant from Tn903. The resulting recombinant plasmid, pUGLG1: kan, was transformed into P. chrysosporium with the expectation that the disrupted gene might replace the homologous LIP gene in the chromosome. However, the results showed that pUGLG1: kan sequences do not integrate into the chromosome; instead, the plasmid is maintained intact in the transformants in an extrachromosomal state. Our data also show that pUGLG1: kan undergoes replication in P. chrysosporium, is maintained as a circular element, is recoverable from meiotic and mitotic progeny, although at a low frequency, and can be recovered intact by Escherichia coli transformation. These results suggest that the GLG1 component of pUGLG1: kan contains as yet unidentified sequences that allow its autonomous replication in P. chrysosporium transformants.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1879693</PMID>
<DateCompleted><Year>1991</Year>
<Month>10</Month>
<Day>03</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>07</Month>
<Day>07</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0378-1119</ISSN>
<JournalIssue CitedMedium="Print"><Volume>103</Volume>
<Issue>1</Issue>
<PubDate><Year>1991</Year>
<Month>Jul</Month>
<Day>15</Day>
</PubDate>
</JournalIssue>
<Title>Gene</Title>
<ISOAbbreviation>Gene</ISOAbbreviation>
</Journal>
<ArticleTitle>An improved transformation vector for the lignin-degrading white rot basidiomycete Phanerochaete chrysosporium.</ArticleTitle>
<Pagination><MedlinePgn>125-30</MedlinePgn>
</Pagination>
<Abstract><AbstractText>In this study, a lignin peroxidase-encoding gene (LIP) of Phanerochaete chrysosporium was disrupted by inserting into its coding region the kanamycin-resistance determinant from Tn903. The resulting recombinant plasmid, pUGLG1: kan, was transformed into P. chrysosporium with the expectation that the disrupted gene might replace the homologous LIP gene in the chromosome. However, the results showed that pUGLG1: kan sequences do not integrate into the chromosome; instead, the plasmid is maintained intact in the transformants in an extrachromosomal state. Our data also show that pUGLG1: kan undergoes replication in P. chrysosporium, is maintained as a circular element, is recoverable from meiotic and mitotic progeny, although at a low frequency, and can be recovered intact by Escherichia coli transformation. These results suggest that the GLG1 component of pUGLG1: kan contains as yet unidentified sequences that allow its autonomous replication in P. chrysosporium transformants.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Randall</LastName>
<ForeName>T</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.</Affiliation>
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<Author ValidYN="Y"><LastName>Reddy</LastName>
<ForeName>C A</ForeName>
<Initials>CA</Initials>
</Author>
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<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>1-RO1-GM39032-01A1</GrantID>
<Acronym>GM</Acronym>
<Agency>NIGMS NIH HHS</Agency>
<Country>United States</Country>
</Grant>
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<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
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<MedlineJournalInfo><Country>Netherlands</Country>
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<ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber>
<NameOfSubstance UI="D008031">Lignin</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber>
<NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber>
<NameOfSubstance UI="C042858">lignin peroxidase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<GeneSymbolList><GeneSymbol>LIP2</GeneSymbol>
</GeneSymbolList>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015139" MajorTopicYN="N">Blotting, Southern</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007613" MajorTopicYN="N">Kanamycin Resistance</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
</MeshHeadingList>
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<Month>7</Month>
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