Lignin peroxidase from the basidiomycete Phanerochaete chrysosporium is synthesized as a preproenzyme.
Identifieur interne : 000E99 ( Main/Corpus ); précédent : 000E98; suivant : 000F00Lignin peroxidase from the basidiomycete Phanerochaete chrysosporium is synthesized as a preproenzyme.
Auteurs : T G Ritch ; V J Nipper ; L. Akileswaran ; A J Smith ; D G Pribnow ; M H GoldSource :
- Gene [ 0378-1119 ] ; 1991.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Basidiomycota (enzymology), Basidiomycota (genetics), Cloning, Molecular (MeSH), Enzyme Precursors (genetics), Enzyme Precursors (metabolism), Isoenzymes (genetics), Isoenzymes (metabolism), Molecular Sequence Data (MeSH), Peroxidases (biosynthesis), Peroxidases (chemistry), Peroxidases (genetics), Protein Biosynthesis (genetics), Protein Processing, Post-Translational (physiology), Protein Sorting Signals (genetics), Protein Sorting Signals (metabolism).
- MESH :
- chemical , biosynthesis : Peroxidases.
- chemical , chemistry : Peroxidases.
- chemical , genetics : Enzyme Precursors, Isoenzymes, Peroxidases, Protein Sorting Signals.
- enzymology : Basidiomycota.
- genetics : Basidiomycota, Protein Biosynthesis.
- chemical , metabolism : Enzyme Precursors, Isoenzymes, Protein Sorting Signals.
- physiology : Protein Processing, Post-Translational.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Molecular Sequence Data.
Abstract
The cDNA clone L18 encoding lignin peroxidase LiP2, the most highly expressed LiP isozyme from Phanerochaete chrysosporium strain OGC101, was isolated and sequenced. Comparison of the cDNA sequence with the N-terminal sequence of the mature LiP2 protein isolated from culture medium suggests that the mature protein contains 343 amino acids (aa) and is preceded by a 28-aa leader sequence. In vitro transcription followed by in vitro translation and processing by signal peptidase resulted in cleavage at a site following the Ala21 (counted from the N-terminal Met1 of the initial translation product). The resultant protein contains a 7-aa propeptide, indicating that LiP is synthesized as a preproenzyme.
DOI: 10.1016/0378-1119(91)90304-t
PubMed: 1743510
Links to Exploration step
pubmed:1743510Le document en format XML
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<author><name sortKey="Ritch, T G" sort="Ritch, T G" uniqKey="Ritch T" first="T G" last="Ritch">T G Ritch</name>
<affiliation><nlm:affiliation>Department of Chemical and Biological Sciences, Oregon Graduate Institute for Science and Technology, Beaverton 97006-1999.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Nipper, V J" sort="Nipper, V J" uniqKey="Nipper V" first="V J" last="Nipper">V J Nipper</name>
</author>
<author><name sortKey="Akileswaran, L" sort="Akileswaran, L" uniqKey="Akileswaran L" first="L" last="Akileswaran">L. Akileswaran</name>
</author>
<author><name sortKey="Smith, A J" sort="Smith, A J" uniqKey="Smith A" first="A J" last="Smith">A J Smith</name>
</author>
<author><name sortKey="Pribnow, D G" sort="Pribnow, D G" uniqKey="Pribnow D" first="D G" last="Pribnow">D G Pribnow</name>
</author>
<author><name sortKey="Gold, M H" sort="Gold, M H" uniqKey="Gold M" first="M H" last="Gold">M H Gold</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Lignin peroxidase from the basidiomycete Phanerochaete chrysosporium is synthesized as a preproenzyme.</title>
<author><name sortKey="Ritch, T G" sort="Ritch, T G" uniqKey="Ritch T" first="T G" last="Ritch">T G Ritch</name>
<affiliation><nlm:affiliation>Department of Chemical and Biological Sciences, Oregon Graduate Institute for Science and Technology, Beaverton 97006-1999.</nlm:affiliation>
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<author><name sortKey="Nipper, V J" sort="Nipper, V J" uniqKey="Nipper V" first="V J" last="Nipper">V J Nipper</name>
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<author><name sortKey="Akileswaran, L" sort="Akileswaran, L" uniqKey="Akileswaran L" first="L" last="Akileswaran">L. Akileswaran</name>
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<author><name sortKey="Smith, A J" sort="Smith, A J" uniqKey="Smith A" first="A J" last="Smith">A J Smith</name>
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<author><name sortKey="Pribnow, D G" sort="Pribnow, D G" uniqKey="Pribnow D" first="D G" last="Pribnow">D G Pribnow</name>
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<author><name sortKey="Gold, M H" sort="Gold, M H" uniqKey="Gold M" first="M H" last="Gold">M H Gold</name>
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<series><title level="j">Gene</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Base Sequence (MeSH)</term>
<term>Basidiomycota (enzymology)</term>
<term>Basidiomycota (genetics)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Enzyme Precursors (genetics)</term>
<term>Enzyme Precursors (metabolism)</term>
<term>Isoenzymes (genetics)</term>
<term>Isoenzymes (metabolism)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Peroxidases (biosynthesis)</term>
<term>Peroxidases (chemistry)</term>
<term>Peroxidases (genetics)</term>
<term>Protein Biosynthesis (genetics)</term>
<term>Protein Processing, Post-Translational (physiology)</term>
<term>Protein Sorting Signals (genetics)</term>
<term>Protein Sorting Signals (metabolism)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Peroxidases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Peroxidases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Enzyme Precursors</term>
<term>Isoenzymes</term>
<term>Peroxidases</term>
<term>Protein Sorting Signals</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Basidiomycota</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
<term>Protein Biosynthesis</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Enzyme Precursors</term>
<term>Isoenzymes</term>
<term>Protein Sorting Signals</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Protein Processing, Post-Translational</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Cloning, Molecular</term>
<term>Molecular Sequence Data</term>
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<front><div type="abstract" xml:lang="en">The cDNA clone L18 encoding lignin peroxidase LiP2, the most highly expressed LiP isozyme from Phanerochaete chrysosporium strain OGC101, was isolated and sequenced. Comparison of the cDNA sequence with the N-terminal sequence of the mature LiP2 protein isolated from culture medium suggests that the mature protein contains 343 amino acids (aa) and is preceded by a 28-aa leader sequence. In vitro transcription followed by in vitro translation and processing by signal peptidase resulted in cleavage at a site following the Ala21 (counted from the N-terminal Met1 of the initial translation product). The resultant protein contains a 7-aa propeptide, indicating that LiP is synthesized as a preproenzyme.</div>
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<Abstract><AbstractText>The cDNA clone L18 encoding lignin peroxidase LiP2, the most highly expressed LiP isozyme from Phanerochaete chrysosporium strain OGC101, was isolated and sequenced. Comparison of the cDNA sequence with the N-terminal sequence of the mature LiP2 protein isolated from culture medium suggests that the mature protein contains 343 amino acids (aa) and is preceded by a 28-aa leader sequence. In vitro transcription followed by in vitro translation and processing by signal peptidase resulted in cleavage at a site following the Ala21 (counted from the N-terminal Met1 of the initial translation product). The resultant protein contains a 7-aa propeptide, indicating that LiP is synthesized as a preproenzyme.</AbstractText>
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