Cloning, sequencing, and heterologous expression of a cellulase-encoding cDNA (cbh1) from Penicillium janthinellum.
Identifieur interne : 000E23 ( Main/Corpus ); précédent : 000E22; suivant : 000E24Cloning, sequencing, and heterologous expression of a cellulase-encoding cDNA (cbh1) from Penicillium janthinellum.
Auteurs : A. Koch ; C T Weigel ; G. SchulzSource :
- Gene [ 0378-1119 ] ; 1993.
English descriptors
- KwdEn :
- Agaricales (enzymology), Agaricales (genetics), Amino Acid Sequence (MeSH), Bacteriophage T7 (genetics), Base Sequence (MeSH), Cellulase (genetics), Cloning, Molecular (MeSH), DNA, Fungal (genetics), Escherichia coli (genetics), Genes, Fungal (MeSH), Mitosporic Fungi (enzymology), Mitosporic Fungi (genetics), Molecular Sequence Data (MeSH), Penicillium (enzymology), Penicillium (genetics), Saccharomyces cerevisiae (enzymology), Saccharomyces cerevisiae (genetics), Sequence Homology, Amino Acid (MeSH), Sequence Homology, Nucleic Acid (MeSH), Trichoderma (enzymology), Trichoderma (genetics).
- MESH :
- chemical , genetics : Cellulase, DNA, Fungal.
- enzymology : Agaricales, Mitosporic Fungi, Penicillium, Saccharomyces cerevisiae, Trichoderma.
- genetics : Agaricales, Bacteriophage T7, Escherichia coli, Mitosporic Fungi, Penicillium, Saccharomyces cerevisiae, Trichoderma.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Genes, Fungal, Molecular Sequence Data, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid.
Abstract
From a Penicillium janthinellum cDNA library, two clones with 1.8- and 1.9-kb inserts were isolated by hybridization to a Trichoderma reesei cellulase-encoding gene probe (egl1). Both cDNAs have identical 5' ends and coding sequences, but different polyadenylation start points in their 3' untranslated regions. In the nucleotide (nt) sequence, one open reading frame of 537 amino acids was detected which shows 56% homology with endoglucanase I of T. reesei and 70% homology with cellobiohydrolase I of T. reesei, Phanerochaete chrysosporium, and Humicola grisea. Expression of the 1.9-kb cDNA in the Escherichia coli T7 system led to the detection of a 57-kDa protein, in agreement with the theoretical value. Fusion to the promoter of the yeast phosphoglycerokinase-encoding gene led to efficient expression and partial secretion of the cDNA-encoded cellulase with cellobiohydrolase I activity in Saccharomyces cerevisiae.
DOI: 10.1016/0378-1119(93)90761-q
PubMed: 8440481
Links to Exploration step
pubmed:8440481Le document en format XML
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<author><name sortKey="Koch, A" sort="Koch, A" uniqKey="Koch A" first="A" last="Koch">A. Koch</name>
<affiliation><nlm:affiliation>Institut für Mikrobiologie, Humboldt-Universität zu Berlin, Kleinmachnow, Germany.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Weigel, C T" sort="Weigel, C T" uniqKey="Weigel C" first="C T" last="Weigel">C T Weigel</name>
</author>
<author><name sortKey="Schulz, G" sort="Schulz, G" uniqKey="Schulz G" first="G" last="Schulz">G. Schulz</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning, sequencing, and heterologous expression of a cellulase-encoding cDNA (cbh1) from Penicillium janthinellum.</title>
<author><name sortKey="Koch, A" sort="Koch, A" uniqKey="Koch A" first="A" last="Koch">A. Koch</name>
<affiliation><nlm:affiliation>Institut für Mikrobiologie, Humboldt-Universität zu Berlin, Kleinmachnow, Germany.</nlm:affiliation>
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<author><name sortKey="Weigel, C T" sort="Weigel, C T" uniqKey="Weigel C" first="C T" last="Weigel">C T Weigel</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agaricales (enzymology)</term>
<term>Agaricales (genetics)</term>
<term>Amino Acid Sequence (MeSH)</term>
<term>Bacteriophage T7 (genetics)</term>
<term>Base Sequence (MeSH)</term>
<term>Cellulase (genetics)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>DNA, Fungal (genetics)</term>
<term>Escherichia coli (genetics)</term>
<term>Genes, Fungal (MeSH)</term>
<term>Mitosporic Fungi (enzymology)</term>
<term>Mitosporic Fungi (genetics)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Penicillium (enzymology)</term>
<term>Penicillium (genetics)</term>
<term>Saccharomyces cerevisiae (enzymology)</term>
<term>Saccharomyces cerevisiae (genetics)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
<term>Sequence Homology, Nucleic Acid (MeSH)</term>
<term>Trichoderma (enzymology)</term>
<term>Trichoderma (genetics)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cellulase</term>
<term>DNA, Fungal</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Agaricales</term>
<term>Mitosporic Fungi</term>
<term>Penicillium</term>
<term>Saccharomyces cerevisiae</term>
<term>Trichoderma</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agaricales</term>
<term>Bacteriophage T7</term>
<term>Escherichia coli</term>
<term>Mitosporic Fungi</term>
<term>Penicillium</term>
<term>Saccharomyces cerevisiae</term>
<term>Trichoderma</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Cloning, Molecular</term>
<term>Genes, Fungal</term>
<term>Molecular Sequence Data</term>
<term>Sequence Homology, Amino Acid</term>
<term>Sequence Homology, Nucleic Acid</term>
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<front><div type="abstract" xml:lang="en">From a Penicillium janthinellum cDNA library, two clones with 1.8- and 1.9-kb inserts were isolated by hybridization to a Trichoderma reesei cellulase-encoding gene probe (egl1). Both cDNAs have identical 5' ends and coding sequences, but different polyadenylation start points in their 3' untranslated regions. In the nucleotide (nt) sequence, one open reading frame of 537 amino acids was detected which shows 56% homology with endoglucanase I of T. reesei and 70% homology with cellobiohydrolase I of T. reesei, Phanerochaete chrysosporium, and Humicola grisea. Expression of the 1.9-kb cDNA in the Escherichia coli T7 system led to the detection of a 57-kDa protein, in agreement with the theoretical value. Fusion to the promoter of the yeast phosphoglycerokinase-encoding gene led to efficient expression and partial secretion of the cDNA-encoded cellulase with cellobiohydrolase I activity in Saccharomyces cerevisiae.</div>
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<Issue>1</Issue>
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<ArticleTitle>Cloning, sequencing, and heterologous expression of a cellulase-encoding cDNA (cbh1) from Penicillium janthinellum.</ArticleTitle>
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<Abstract><AbstractText>From a Penicillium janthinellum cDNA library, two clones with 1.8- and 1.9-kb inserts were isolated by hybridization to a Trichoderma reesei cellulase-encoding gene probe (egl1). Both cDNAs have identical 5' ends and coding sequences, but different polyadenylation start points in their 3' untranslated regions. In the nucleotide (nt) sequence, one open reading frame of 537 amino acids was detected which shows 56% homology with endoglucanase I of T. reesei and 70% homology with cellobiohydrolase I of T. reesei, Phanerochaete chrysosporium, and Humicola grisea. Expression of the 1.9-kb cDNA in the Escherichia coli T7 system led to the detection of a 57-kDa protein, in agreement with the theoretical value. Fusion to the promoter of the yeast phosphoglycerokinase-encoding gene led to efficient expression and partial secretion of the cDNA-encoded cellulase with cellobiohydrolase I activity in Saccharomyces cerevisiae.</AbstractText>
</Abstract>
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<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
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<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
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<MeshHeading><DescriptorName UI="D010407" MajorTopicYN="N">Penicillium</DescriptorName>
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<MeshHeading><DescriptorName UI="D012689" MajorTopicYN="N">Sequence Homology, Nucleic Acid</DescriptorName>
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<MeshHeading><DescriptorName UI="D014242" MajorTopicYN="N">Trichoderma</DescriptorName>
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