Alternative methods for production and staining of Phanerochaete chrysosporium basidiospores.
Identifieur interne : 000E13 ( Main/Corpus ); précédent : 000E12; suivant : 000E14Alternative methods for production and staining of Phanerochaete chrysosporium basidiospores.
Auteurs : S S Dhawale ; K. KesslerSource :
- Applied and environmental microbiology [ 0099-2240 ] ; 1993.
English descriptors
- KwdEn :
- MESH :
- chemical : Indoles.
- methods : Mycology, Staining and Labeling.
- physiology : Basidiomycota, Spores, Fungal.
- ultrastructure : Basidiomycota, Cell Nucleus, Spores, Fungal.
- Evaluation Studies as Topic.
Abstract
Homokaryotic isolates of Phanerochaete chrysosporium are generally obtained by stimulating the production of basidiospores. The most commonly used method requires a special incubator that is maintained at 28 degrees C with continuous illumination. Here we report an alternate method which permits the production of basidiopores with common laboratory incubators and requires no special illumination conditions. This alternate method gives reproducible results and yields basidiospores that are not contaminated with conidia. We also report a detailed optimized method for staining basidiospores for visualizing nuclei.
DOI: 10.1128/AEM.59.5.1675-1677.1993
PubMed: 7686003
PubMed Central: PMC182137
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pubmed:7686003Le document en format XML
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Kessler</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1993">1993</date><idno type="RBID">pubmed:7686003</idno><idno type="pmid">7686003</idno><idno type="pmc">PMC182137</idno><idno type="doi">10.1128/AEM.59.5.1675-1677.1993</idno><idno type="wicri:Area/Main/Corpus">000E13</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000E13</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Alternative methods for production and staining of Phanerochaete chrysosporium basidiospores.</title><author><name sortKey="Dhawale, S S" sort="Dhawale, S S" uniqKey="Dhawale S" first="S S" last="Dhawale">S S Dhawale</name><affiliation><nlm:affiliation>Department of Biology, Indiana University-Purdue University, Fort Wayne 46805.</nlm:affiliation></affiliation></author><author><name sortKey="Kessler, K" sort="Kessler, K" uniqKey="Kessler K" first="K" last="Kessler">K. Kessler</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="ISSN">0099-2240</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (physiology)</term><term>Basidiomycota (ultrastructure)</term><term>Cell Nucleus (ultrastructure)</term><term>Evaluation Studies as Topic (MeSH)</term><term>Indoles (MeSH)</term><term>Mycology (methods)</term><term>Spores, Fungal (physiology)</term><term>Spores, Fungal (ultrastructure)</term><term>Staining and Labeling (methods)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Indoles</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Mycology</term><term>Staining and Labeling</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Basidiomycota</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Basidiomycota</term><term>Cell Nucleus</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Evaluation Studies as Topic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Homokaryotic isolates of Phanerochaete chrysosporium are generally obtained by stimulating the production of basidiospores. The most commonly used method requires a special incubator that is maintained at 28 degrees C with continuous illumination. Here we report an alternate method which permits the production of basidiopores with common laboratory incubators and requires no special illumination conditions. This alternate method gives reproducible results and yields basidiospores that are not contaminated with conidia. We also report a detailed optimized method for staining basidiospores for visualizing nuclei.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">7686003</PMID><DateCompleted><Year>1993</Year><Month>07</Month><Day>21</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>24</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0099-2240</ISSN><JournalIssue CitedMedium="Print"><Volume>59</Volume><Issue>5</Issue><PubDate><Year>1993</Year><Month>May</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Alternative methods for production and staining of Phanerochaete chrysosporium basidiospores.</ArticleTitle><Pagination><MedlinePgn>1675-7</MedlinePgn></Pagination><Abstract><AbstractText>Homokaryotic isolates of Phanerochaete chrysosporium are generally obtained by stimulating the production of basidiospores. The most commonly used method requires a special incubator that is maintained at 28 degrees C with continuous illumination. Here we report an alternate method which permits the production of basidiopores with common laboratory incubators and requires no special illumination conditions. This alternate method gives reproducible results and yields basidiospores that are not contaminated with conidia. We also report a detailed optimized method for staining basidiospores for visualizing nuclei.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dhawale</LastName><ForeName>S S</ForeName><Initials>SS</Initials><AffiliationInfo><Affiliation>Department of Biology, Indiana University-Purdue University, Fort Wayne 46805.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kessler</LastName><ForeName>K</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1R15GM47556-01</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Environ Microbiol</MedlineTA><NlmUniqueID>7605801</NlmUniqueID><ISSNLinking>0099-2240</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007211">Indoles</NameOfSubstance></Chemical><Chemical><RegistryNumber>47165-04-8</RegistryNumber><NameOfSubstance UI="C007293">DAPI</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005069" MajorTopicYN="N">Evaluation Studies as Topic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007211" MajorTopicYN="N">Indoles</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009172" MajorTopicYN="N">Mycology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013172" MajorTopicYN="N">Spores, Fungal</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013194" MajorTopicYN="N">Staining and Labeling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1993</Year><Month>5</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1993</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1993</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">7686003</ArticleId><ArticleId IdType="pmc">PMC182137</ArticleId><ArticleId IdType="doi">10.1128/AEM.59.5.1675-1677.1993</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>EMBO J. 1986 Jun;5(6):1125-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15966099</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1982 Oct;44(4):996-1000</Citation><ArticleIdList><ArticleId IdType="pubmed">16346124</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1985 Jul;50(1):27-30</Citation><ArticleIdList><ArticleId IdType="pubmed">16346838</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 1987 Jul;53(7):1464-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16347375</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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