A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium.
Identifieur interne : 000D52 ( Main/Corpus ); précédent : 000D51; suivant : 000D53A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium.
Auteurs : M. Gessner ; U. RaederSource :
- Gene [ 0378-1119 ] ; 1994.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Basidiomycota (drug effects), Basidiomycota (genetics), Basidiomycota (growth & development), DNA, Fungal (metabolism), Drug Resistance, Microbial (genetics), Genetic Markers (genetics), Genetic Vectors (MeSH), Histones (genetics), Methylation (MeSH), Phleomycins (pharmacology), Promoter Regions, Genetic (genetics), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Bacterial Proteins, Genetic Markers, Histones.
- drug effects : Basidiomycota.
- genetics : Basidiomycota, Drug Resistance, Microbial, Promoter Regions, Genetic.
- growth & development : Basidiomycota.
- chemical , metabolism : DNA, Fungal.
- chemical , pharmacology : Phleomycins.
- Genetic Vectors, Methylation, Transformation, Genetic.
Abstract
In this study, two transformation vectors (pMG101 and pMG103) for Phanerochaete chrysosporium were constructed, based on the ble phleomycin-resistance-encoding gene and a homologous histone H4 promoter. Transformation frequencies were 6-10 per micrograms of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stably integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding H3 gene in the promoter fragment, becomes methylated, resulting in inactivation of ble-dependent resistance. Plasmid pMG103, which lacks the H3, does not show methylation.
DOI: 10.1016/0378-1119(94)90267-4
PubMed: 8194757
Links to Exploration step
pubmed:8194757Le document en format XML
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<author><name sortKey="Gessner, M" sort="Gessner, M" uniqKey="Gessner M" first="M" last="Gessner">M. Gessner</name>
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<author><name sortKey="Raeder, U" sort="Raeder, U" uniqKey="Raeder U" first="U" last="Raeder">U. Raeder</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (genetics)</term>
<term>Basidiomycota (drug effects)</term>
<term>Basidiomycota (genetics)</term>
<term>Basidiomycota (growth & development)</term>
<term>DNA, Fungal (metabolism)</term>
<term>Drug Resistance, Microbial (genetics)</term>
<term>Genetic Markers (genetics)</term>
<term>Genetic Vectors (MeSH)</term>
<term>Histones (genetics)</term>
<term>Methylation (MeSH)</term>
<term>Phleomycins (pharmacology)</term>
<term>Promoter Regions, Genetic (genetics)</term>
<term>Transformation, Genetic (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term>
<term>Genetic Markers</term>
<term>Histones</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Basidiomycota</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
<term>Drug Resistance, Microbial</term>
<term>Promoter Regions, Genetic</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Basidiomycota</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA, Fungal</term>
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<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Phleomycins</term>
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<front><div type="abstract" xml:lang="en">In this study, two transformation vectors (pMG101 and pMG103) for Phanerochaete chrysosporium were constructed, based on the ble phleomycin-resistance-encoding gene and a homologous histone H4 promoter. Transformation frequencies were 6-10 per micrograms of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stably integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding H3 gene in the promoter fragment, becomes methylated, resulting in inactivation of ble-dependent resistance. Plasmid pMG103, which lacks the H3, does not show methylation.</div>
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<Issue>2</Issue>
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<Month>May</Month>
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<ArticleTitle>A histone H4 promoter for expression of a phleomycin-resistance gene in Phanerochaete chrysosporium.</ArticleTitle>
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<Abstract><AbstractText>In this study, two transformation vectors (pMG101 and pMG103) for Phanerochaete chrysosporium were constructed, based on the ble phleomycin-resistance-encoding gene and a homologous histone H4 promoter. Transformation frequencies were 6-10 per micrograms of DNA. Transformed vector DNA could either exist as an unstable replicating plasmid or could be stably integrated. Integrated vector DNA from pMG101, which also contains a histone-encoding H3 gene in the promoter fragment, becomes methylated, resulting in inactivation of ble-dependent resistance. Plasmid pMG103, which lacks the H3, does not show methylation.</AbstractText>
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