Metabolism of cellulose by Phanerochaete chrysosporium in continuously agitated culture is associated with enhanced production of lignin peroxidase.
Identifieur interne : 000B03 ( Main/Corpus ); précédent : 000B02; suivant : 000B04Metabolism of cellulose by Phanerochaete chrysosporium in continuously agitated culture is associated with enhanced production of lignin peroxidase.
Auteurs : L. Zacchi ; G. Burla ; D. Zuolong ; P J HarveySource :
- Journal of biotechnology [ 0168-1656 ] ; 2000.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Peroxidases.
- chemical , metabolism : Cellulose, Glucose.
- chemical , pharmacology : Oxygen.
- growth & development : Phanerochaete.
- metabolism : Phanerochaete.
- Oxygen Consumption.
Abstract
Production of the extracellular heme protein lignin peroxidase (LiP) by Phanerochaete chrysosporium is currently associated with a number of requirements, namely exposure of the cultures to oxygen; limiting nutrient nitrogen or carbon and static or semi-static culture conditions. To obtain LiP activity in continuously agitated liquid culture requires the inclusion of a surfactant. However, using cellulose as the carbon source, we obtained high titres (0.2-0.4 U ml(-1)) of LiP in submerged liquid cultures under conditions of continuous agitation, without substrate limitation or the need to add oxygen or surfactant. Comparison of the morphological and physiological traits of hyphae maintained on either cellulose or free glucose supports observations that the synthesis of extracellular polysaccharide in the cultures grown on glucose, restricts oxygen diffusion into the hyphae, which is necessary for LiP induction. They also suggest that isozymes of LiP synthesised under these conditions may be triggered in response to oxidant stress.
DOI: 10.1016/s0168-1656(00)00209-1
PubMed: 10725541
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pubmed:10725541Le document en format XML
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Zuolong</name></author><author><name sortKey="Harvey, P J" sort="Harvey, P J" uniqKey="Harvey P" first="P J" last="Harvey">P J Harvey</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:10725541</idno><idno type="pmid">10725541</idno><idno type="doi">10.1016/s0168-1656(00)00209-1</idno><idno type="wicri:Area/Main/Corpus">000B03</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000B03</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Metabolism of cellulose by Phanerochaete chrysosporium in continuously agitated culture is associated with enhanced production of lignin peroxidase.</title><author><name sortKey="Zacchi, L" sort="Zacchi, L" uniqKey="Zacchi L" first="L" last="Zacchi">L. Zacchi</name><affiliation><nlm:affiliation>School of Chemical and Life Sciences, University of Greenwich, Wellington Street, London, UK.</nlm:affiliation></affiliation></author><author><name sortKey="Burla, G" sort="Burla, G" uniqKey="Burla G" first="G" last="Burla">G. Burla</name></author><author><name sortKey="Zuolong, D" sort="Zuolong, D" uniqKey="Zuolong D" first="D" last="Zuolong">D. Zuolong</name></author><author><name sortKey="Harvey, P J" sort="Harvey, P J" uniqKey="Harvey P" first="P J" last="Harvey">P J Harvey</name></author></analytic><series><title level="j">Journal of biotechnology</title><idno type="ISSN">0168-1656</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cellulose (metabolism)</term><term>Glucose (metabolism)</term><term>Oxygen (pharmacology)</term><term>Oxygen Consumption (MeSH)</term><term>Peroxidases (biosynthesis)</term><term>Phanerochaete (growth & development)</term><term>Phanerochaete (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Peroxidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulose</term><term>Glucose</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Oxygen</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Oxygen Consumption</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Production of the extracellular heme protein lignin peroxidase (LiP) by Phanerochaete chrysosporium is currently associated with a number of requirements, namely exposure of the cultures to oxygen; limiting nutrient nitrogen or carbon and static or semi-static culture conditions. To obtain LiP activity in continuously agitated liquid culture requires the inclusion of a surfactant. However, using cellulose as the carbon source, we obtained high titres (0.2-0.4 U ml(-1)) of LiP in submerged liquid cultures under conditions of continuous agitation, without substrate limitation or the need to add oxygen or surfactant. Comparison of the morphological and physiological traits of hyphae maintained on either cellulose or free glucose supports observations that the synthesis of extracellular polysaccharide in the cultures grown on glucose, restricts oxygen diffusion into the hyphae, which is necessary for LiP induction. They also suggest that isozymes of LiP synthesised under these conditions may be triggered in response to oxidant stress.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10725541</PMID><DateCompleted><Year>2000</Year><Month>05</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0168-1656</ISSN><JournalIssue CitedMedium="Print"><Volume>78</Volume><Issue>2</Issue><PubDate><Year>2000</Year><Month>Mar</Month><Day>10</Day></PubDate></JournalIssue><Title>Journal of biotechnology</Title><ISOAbbreviation>J Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Metabolism of cellulose by Phanerochaete chrysosporium in continuously agitated culture is associated with enhanced production of lignin peroxidase.</ArticleTitle><Pagination><MedlinePgn>185-92</MedlinePgn></Pagination><Abstract><AbstractText>Production of the extracellular heme protein lignin peroxidase (LiP) by Phanerochaete chrysosporium is currently associated with a number of requirements, namely exposure of the cultures to oxygen; limiting nutrient nitrogen or carbon and static or semi-static culture conditions. To obtain LiP activity in continuously agitated liquid culture requires the inclusion of a surfactant. However, using cellulose as the carbon source, we obtained high titres (0.2-0.4 U ml(-1)) of LiP in submerged liquid cultures under conditions of continuous agitation, without substrate limitation or the need to add oxygen or surfactant. Comparison of the morphological and physiological traits of hyphae maintained on either cellulose or free glucose supports observations that the synthesis of extracellular polysaccharide in the cultures grown on glucose, restricts oxygen diffusion into the hyphae, which is necessary for LiP induction. They also suggest that isozymes of LiP synthesised under these conditions may be triggered in response to oxidant stress.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zacchi</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>School of Chemical and Life Sciences, University of Greenwich, Wellington Street, London, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Burla</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Zuolong</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Harvey</LastName><ForeName>P J</ForeName><Initials>PJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>J Biotechnol</MedlineTA><NlmUniqueID>8411927</NlmUniqueID><ISSNLinking>0168-1656</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="C042858">lignin peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>IY9XDZ35W2</RegistryNumber><NameOfSubstance UI="D005947">Glucose</NameOfSubstance></Chemical><Chemical><RegistryNumber>S88TT14065</RegistryNumber><NameOfSubstance UI="D010100">Oxygen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010100" MajorTopicYN="N">Oxygen</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010101" MajorTopicYN="N">Oxygen Consumption</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2000</Year><Month>3</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2000</Year><Month>5</Month><Day>8</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2000</Year><Month>3</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">10725541</ArticleId><ArticleId IdType="pii">S0168-1656(00)00209-1</ArticleId><ArticleId IdType="doi">10.1016/s0168-1656(00)00209-1</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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