An amperometric cellobiose dehydrogenase-based biosensor can be used for measurement of cellulase activity.
Identifieur interne : 000A66 ( Main/Corpus ); précédent : 000A65; suivant : 000A67An amperometric cellobiose dehydrogenase-based biosensor can be used for measurement of cellulase activity.
Auteurs : L. Hildén ; L. Eng ; G. Johansson ; S E Lindqvist ; G. PetterssonSource :
- Analytical biochemistry [ 0003-2697 ] ; 2001.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Cellulase.
- chemical , chemistry : Carbohydrate Dehydrogenases.
- enzymology : Phanerochaete.
- methods : Biosensing Techniques.
- Evaluation Studies as Topic, Quality Control, Reproducibility of Results, Time Factors.
Abstract
The hemoflavoenzyme cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. Used in conjugation with a FIA system this biosensor can replace colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. The biosensor gave the same result as the Somogyi-Nelson method in a less time-consuming and laborious manner. The two methods showed about the same precision.
DOI: 10.1006/abio.2000.4959
PubMed: 11237326
Links to Exploration step
pubmed:11237326Le document en format XML
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<author><name sortKey="Hilden, L" sort="Hilden, L" uniqKey="Hilden L" first="L" last="Hildén">L. Hildén</name>
<affiliation><nlm:affiliation>Department of Biochemistry, Uppsala University, SE-751 23 Uppsala, Sweden. lars.hilden@biokem.uu.se</nlm:affiliation>
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<author><name sortKey="Eng, L" sort="Eng, L" uniqKey="Eng L" first="L" last="Eng">L. Eng</name>
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<author><name sortKey="Johansson, G" sort="Johansson, G" uniqKey="Johansson G" first="G" last="Johansson">G. Johansson</name>
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<author><name sortKey="Lindqvist, S E" sort="Lindqvist, S E" uniqKey="Lindqvist S" first="S E" last="Lindqvist">S E Lindqvist</name>
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<author><name sortKey="Pettersson, G" sort="Pettersson, G" uniqKey="Pettersson G" first="G" last="Pettersson">G. Pettersson</name>
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<author><name sortKey="Hilden, L" sort="Hilden, L" uniqKey="Hilden L" first="L" last="Hildén">L. Hildén</name>
<affiliation><nlm:affiliation>Department of Biochemistry, Uppsala University, SE-751 23 Uppsala, Sweden. lars.hilden@biokem.uu.se</nlm:affiliation>
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<author><name sortKey="Eng, L" sort="Eng, L" uniqKey="Eng L" first="L" last="Eng">L. Eng</name>
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<author><name sortKey="Johansson, G" sort="Johansson, G" uniqKey="Johansson G" first="G" last="Johansson">G. Johansson</name>
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<author><name sortKey="Lindqvist, S E" sort="Lindqvist, S E" uniqKey="Lindqvist S" first="S E" last="Lindqvist">S E Lindqvist</name>
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<author><name sortKey="Pettersson, G" sort="Pettersson, G" uniqKey="Pettersson G" first="G" last="Pettersson">G. Pettersson</name>
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<series><title level="j">Analytical biochemistry</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (methods)</term>
<term>Carbohydrate Dehydrogenases (chemistry)</term>
<term>Cellulase (analysis)</term>
<term>Evaluation Studies as Topic (MeSH)</term>
<term>Phanerochaete (enzymology)</term>
<term>Quality Control (MeSH)</term>
<term>Reproducibility of Results (MeSH)</term>
<term>Time Factors (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Cellulase</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Carbohydrate Dehydrogenases</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term>
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<keywords scheme="MESH" xml:lang="en"><term>Evaluation Studies as Topic</term>
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<front><div type="abstract" xml:lang="en">The hemoflavoenzyme cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. Used in conjugation with a FIA system this biosensor can replace colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. The biosensor gave the same result as the Somogyi-Nelson method in a less time-consuming and laborious manner. The two methods showed about the same precision.</div>
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<Day>15</Day>
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<Issue>2</Issue>
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<Month>Mar</Month>
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<Title>Analytical biochemistry</Title>
<ISOAbbreviation>Anal Biochem</ISOAbbreviation>
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<ArticleTitle>An amperometric cellobiose dehydrogenase-based biosensor can be used for measurement of cellulase activity.</ArticleTitle>
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<Abstract><AbstractText>The hemoflavoenzyme cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. Used in conjugation with a FIA system this biosensor can replace colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. The biosensor gave the same result as the Somogyi-Nelson method in a less time-consuming and laborious manner. The two methods showed about the same precision.</AbstractText>
<CopyrightInformation>Copyright 2001 Academic Press.</CopyrightInformation>
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