Incomplete processing of peroxidase transcripts in the lignin degrading fungus Phanerochaete chrysosporium.
Identifieur interne : 000866 ( Main/Corpus ); précédent : 000865; suivant : 000867Incomplete processing of peroxidase transcripts in the lignin degrading fungus Phanerochaete chrysosporium.
Auteurs : Stuardo Macarena ; Larrondo Luis Fernando ; Vásquez M Nica ; Vicu A Rafael ; González BernardoSource :
- FEMS microbiology letters [ 0378-1097 ] ; 2005.
English descriptors
- KwdEn :
- Codon, Terminator (physiology), DNA, Complementary (chemistry), DNA, Fungal (chemistry), Fungal Proteins (genetics), Gene Expression Regulation, Fungal (MeSH), Introns (MeSH), Lignin (metabolism), Peroxidases (biosynthesis), Peroxidases (genetics), Phanerochaete (genetics), Phanerochaete (metabolism), RNA Processing, Post-Transcriptional (MeSH), RNA, Fungal (genetics), RNA, Fungal (isolation & purification), RNA, Fungal (metabolism), RNA, Messenger (genetics), RNA, Messenger (isolation & purification), RNA, Messenger (metabolism), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Sequence Analysis, DNA (MeSH).
- MESH :
- chemical , biosynthesis : Peroxidases.
- chemical , chemistry : DNA, Complementary, DNA, Fungal.
- chemical , genetics : Fungal Proteins, Peroxidases, RNA, Fungal, RNA, Messenger.
- chemical , isolation & purification : RNA, Fungal, RNA, Messenger.
- chemical , metabolism : Lignin, RNA, Fungal, RNA, Messenger.
- chemical , physiology : Codon, Terminator.
- genetics : Phanerochaete.
- metabolism : Phanerochaete.
- Gene Expression Regulation, Fungal, Introns, RNA Processing, Post-Transcriptional, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA.
Abstract
Phanerochaete chrysosporium has been thoroughly studied as a microbial model for lignin degradation. The enzymes lignin peroxidase (LiP) and manganese peroxidase (MnP), both encoded by several genes, play the main role in the cleavage of different lignin substructures. In this work, the expression of specific LiP and MnP transcripts in liquid medium and in a wood-containing soil system was studied by reverse transcription-PCR and subsequent cloning and sequencing of the products obtained. Splice variants of different LiP and MnP transcripts were observed in wood-containing soil incubations and in liquid cultures. The processed transcripts contained different numbers of complete introns. Since the presence of stop codons in several of these introns would prevent the synthesis of active enzyme, we propose that these transcripts arise as a result of incomplete processing rather than alternative splicing. Interestingly, analysis of splice variants from mnp genes led to the identification of a fourth actively transcribed gene coding for MnP in P. chrysosporium.
DOI: 10.1016/j.femsle.2004.10.037
PubMed: 15621417
Links to Exploration step
pubmed:15621417Le document en format XML
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Vicu A" uniqKey="Rafael V" first="Vicu A" last="Rafael">Vicu A Rafael</name></author><author><name sortKey="Bernardo, Gonzalez" sort="Bernardo, Gonzalez" uniqKey="Bernardo G" first="González" last="Bernardo">González Bernardo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15621417</idno><idno type="pmid">15621417</idno><idno type="doi">10.1016/j.femsle.2004.10.037</idno><idno type="wicri:Area/Main/Corpus">000866</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000866</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Incomplete processing of peroxidase transcripts in the lignin degrading fungus Phanerochaete chrysosporium.</title><author><name sortKey="Macarena, Stuardo" sort="Macarena, Stuardo" uniqKey="Macarena S" first="Stuardo" last="Macarena">Stuardo Macarena</name><affiliation><nlm:affiliation>Laboratorio de 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(MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Peroxidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term><term>DNA, Fungal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>Peroxidases</term><term>RNA, Fungal</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Fungal</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term><term>RNA, Fungal</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Codon, Terminator</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Fungal</term><term>Introns</term><term>RNA Processing, Post-Transcriptional</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phanerochaete chrysosporium has been thoroughly studied as a microbial model for lignin degradation. The enzymes lignin peroxidase (LiP) and manganese peroxidase (MnP), both encoded by several genes, play the main role in the cleavage of different lignin substructures. In this work, the expression of specific LiP and MnP transcripts in liquid medium and in a wood-containing soil system was studied by reverse transcription-PCR and subsequent cloning and sequencing of the products obtained. Splice variants of different LiP and MnP transcripts were observed in wood-containing soil incubations and in liquid cultures. The processed transcripts contained different numbers of complete introns. Since the presence of stop codons in several of these introns would prevent the synthesis of active enzyme, we propose that these transcripts arise as a result of incomplete processing rather than alternative splicing. Interestingly, analysis of splice variants from mnp genes led to the identification of a fourth actively transcribed gene coding for MnP in P. chrysosporium.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15621417</PMID><DateCompleted><Year>2005</Year><Month>06</Month><Day>14</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0378-1097</ISSN><JournalIssue CitedMedium="Print"><Volume>242</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Jan</Month><Day>01</Day></PubDate></JournalIssue><Title>FEMS microbiology letters</Title><ISOAbbreviation>FEMS Microbiol Lett</ISOAbbreviation></Journal><ArticleTitle>Incomplete processing of peroxidase transcripts in the lignin degrading fungus Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>37-44</MedlinePgn></Pagination><Abstract><AbstractText>Phanerochaete chrysosporium has been thoroughly studied as a microbial model for lignin degradation. The enzymes lignin peroxidase (LiP) and manganese peroxidase (MnP), both encoded by several genes, play the main role in the cleavage of different lignin substructures. In this work, the expression of specific LiP and MnP transcripts in liquid medium and in a wood-containing soil system was studied by reverse transcription-PCR and subsequent cloning and sequencing of the products obtained. Splice variants of different LiP and MnP transcripts were observed in wood-containing soil incubations and in liquid cultures. The processed transcripts contained different numbers of complete introns. Since the presence of stop codons in several of these introns would prevent the synthesis of active enzyme, we propose that these transcripts arise as a result of incomplete processing rather than alternative splicing. Interestingly, analysis of splice variants from mnp genes led to the identification of a fourth actively transcribed gene coding for MnP in P. chrysosporium.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Macarena</LastName><ForeName>Stuardo</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Laboratorio de Microbiología, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, and Millennium Institute for Fundamental and Applied Biology, Santiago, Chile.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fernando</LastName><ForeName>Larrondo Luis</ForeName><Initials>LL</Initials></Author><Author ValidYN="Y"><LastName>Mónica</LastName><ForeName>Vásquez</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Rafael</LastName><ForeName>Vicuña</ForeName><Initials>V</Initials></Author><Author 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