Fungal β-glucosidase expression in Saccharomyces cerevisiae.
Identifieur interne : 000425 ( Main/Corpus ); précédent : 000424; suivant : 000426Fungal β-glucosidase expression in Saccharomyces cerevisiae.
Auteurs : A P Njokweni ; S H Rose ; W H Van ZylSource :
- Journal of industrial microbiology & biotechnology [ 1476-5535 ] ; 2012.
English descriptors
- KwdEn :
- Anaerobiosis (MeSH), Cellobiose (metabolism), Cellulose (metabolism), Chromatography, High Pressure Liquid (MeSH), Ethanol (metabolism), Ethanol (supply & distribution), Fermentation (MeSH), Kluyveromyces (enzymology), Kluyveromyces (genetics), Membrane Transport Proteins (genetics), Membrane Transport Proteins (metabolism), Phanerochaete (enzymology), Phanerochaete (genetics), Recombinant Proteins (biosynthesis), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (growth & development), Saccharomyces cerevisiae (metabolism), Thermoascus (enzymology), Thermoascus (genetics), beta-Glucosidase (biosynthesis), beta-Glucosidase (genetics), beta-Glucosidase (metabolism).
- MESH :
- chemical , biosynthesis : Recombinant Proteins, beta-Glucosidase.
- chemical , genetics : Membrane Transport Proteins, Recombinant Proteins, beta-Glucosidase.
- chemical , metabolism : Cellobiose, Cellulose, Ethanol, Membrane Transport Proteins, Recombinant Proteins, beta-Glucosidase.
- chemical , supply & distribution : Ethanol.
- enzymology : Kluyveromyces, Phanerochaete, Thermoascus.
- genetics : Kluyveromyces, Phanerochaete, Saccharomyces cerevisiae, Thermoascus.
- growth & development : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- Anaerobiosis, Chromatography, High Pressure Liquid, Fermentation.
Abstract
Recombinant Saccharomyces cerevisiae strains expressing β-glucosidases from Thermoascus aurantiacus (Tabgl1) and Phanerochaete chrysosporium (PcbglB and Pccbgl1) were constructed and compared to S. cerevisiae Y294[SFI], previously identified as the best β-glucosidase-producing strain. The PcbglB was also intracellularly expressed in combination with the lac12 lactose permease of Kluyveromyces lactis in S. cerevisiae Y294[PcbglB + Lac12]. The recombinant extracellular β-glucosidases indicated maximum activity in the pH range 4-5 and temperature optima varying from 50 to 75 °C. The S. cerevisiae Y294[Pccbgl1] strain performed best under aerobic and anaerobic conditions, producing 2.6 times more β-glucosidase activity than S. cerevisiae Y294[SFI] and an ethanol concentration of 4.8 g l(-1) after 24 h of cultivation on cellobiose as sole carbohydrate source. S. cerevisiae Y294[Tabgl1] was unable to grow on cellobiose (liquid medium), whereas S. cerevisiae Y294[PcbglB + Lac12] exhibited limited growth.
DOI: 10.1007/s10295-012-1150-9
PubMed: 22707073
Links to Exploration step
pubmed:22707073Le document en format XML
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<term>Chromatography, High Pressure Liquid (MeSH)</term>
<term>Ethanol (metabolism)</term>
<term>Ethanol (supply & distribution)</term>
<term>Fermentation (MeSH)</term>
<term>Kluyveromyces (enzymology)</term>
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<term>Membrane Transport Proteins (genetics)</term>
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<term>Saccharomyces cerevisiae (growth & development)</term>
<term>Saccharomyces cerevisiae (metabolism)</term>
<term>Thermoascus (enzymology)</term>
<term>Thermoascus (genetics)</term>
<term>beta-Glucosidase (biosynthesis)</term>
<term>beta-Glucosidase (genetics)</term>
<term>beta-Glucosidase (metabolism)</term>
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<keywords scheme="MESH" type="chemical" qualifier="supply & distribution" xml:lang="en"><term>Ethanol</term>
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<term>Thermoascus</term>
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<front><div type="abstract" xml:lang="en">Recombinant Saccharomyces cerevisiae strains expressing β-glucosidases from Thermoascus aurantiacus (Tabgl1) and Phanerochaete chrysosporium (PcbglB and Pccbgl1) were constructed and compared to S. cerevisiae Y294[SFI], previously identified as the best β-glucosidase-producing strain. The PcbglB was also intracellularly expressed in combination with the lac12 lactose permease of Kluyveromyces lactis in S. cerevisiae Y294[PcbglB + Lac12]. The recombinant extracellular β-glucosidases indicated maximum activity in the pH range 4-5 and temperature optima varying from 50 to 75 °C. The S. cerevisiae Y294[Pccbgl1] strain performed best under aerobic and anaerobic conditions, producing 2.6 times more β-glucosidase activity than S. cerevisiae Y294[SFI] and an ethanol concentration of 4.8 g l(-1) after 24 h of cultivation on cellobiose as sole carbohydrate source. S. cerevisiae Y294[Tabgl1] was unable to grow on cellobiose (liquid medium), whereas S. cerevisiae Y294[PcbglB + Lac12] exhibited limited growth.</div>
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<Abstract><AbstractText>Recombinant Saccharomyces cerevisiae strains expressing β-glucosidases from Thermoascus aurantiacus (Tabgl1) and Phanerochaete chrysosporium (PcbglB and Pccbgl1) were constructed and compared to S. cerevisiae Y294[SFI], previously identified as the best β-glucosidase-producing strain. The PcbglB was also intracellularly expressed in combination with the lac12 lactose permease of Kluyveromyces lactis in S. cerevisiae Y294[PcbglB + Lac12]. The recombinant extracellular β-glucosidases indicated maximum activity in the pH range 4-5 and temperature optima varying from 50 to 75 °C. The S. cerevisiae Y294[Pccbgl1] strain performed best under aerobic and anaerobic conditions, producing 2.6 times more β-glucosidase activity than S. cerevisiae Y294[SFI] and an ethanol concentration of 4.8 g l(-1) after 24 h of cultivation on cellobiose as sole carbohydrate source. S. cerevisiae Y294[Tabgl1] was unable to grow on cellobiose (liquid medium), whereas S. cerevisiae Y294[PcbglB + Lac12] exhibited limited growth.</AbstractText>
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