Reducing sugars production from corncobs: a comparative study of chemical and biotechnological methods.
Identifieur interne : 000298 ( Main/Corpus ); précédent : 000297; suivant : 000299Reducing sugars production from corncobs: a comparative study of chemical and biotechnological methods.
Auteurs : Ravichandra Potumarthi ; Rama Raju Baadhe ; Aparna Pisipati ; Annapurna JettySource :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2014.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Alkalies, Carbohydrates.
- chemical , metabolism : Cellulase.
- chemistry : Zea mays.
- drug effects : Zea mays.
- enzymology : Phanerochaete.
- methods : Biotechnology.
- chemical , pharmacology : Sodium Hydroxide, Sulfuric Acids.
- Dose-Response Relationship, Drug, Hydrolysis.
Abstract
Two commonly used chemical pretreatment processes, sulphuric acid, and sodium hydroxide, were tested to provide comparative performance data. A connection between solid to liquid ratio (S/L) and sugars released was observed with an increase in S/L ratio between 0.02 and 0.2. Enzymatic digestibility of 1 M of NaOH-pretreated corncobs were released 210.7 mg ml(-1) of sugars. Further, compared with different concentrations of acid pretreatments at 0.05, 0.1, 0.15, 0.2, 0.25, 0.3, and 0.5 M concentrations, sodium hydroxide pretreatment of corncob substantially increased accessibility and digestibility of cellulose. Another additional observation made was whole-cell and crude enzymatic hydrolysis of different concentrations of acid and NaOH (0.05, 0.1, 0.25 M)-treated materials released lower amount of sugars compared with the sugars released (310.9 mg ml(-1)) with whole-cell hydrolysis of 1 M of NaOH-treated corncobs. NaOH-pretreated corncobs contained higher content of sugars and which is more suitable for production of reducing sugars.
DOI: 10.1007/s12010-014-1073-1
PubMed: 25172055
Links to Exploration step
pubmed:25172055Le document en format XML
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<author><name sortKey="Baadhe, Rama Raju" sort="Baadhe, Rama Raju" uniqKey="Baadhe R" first="Rama Raju" last="Baadhe">Rama Raju Baadhe</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Reducing sugars production from corncobs: a comparative study of chemical and biotechnological methods.</title>
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<term>Biotechnology (methods)</term>
<term>Carbohydrates (chemistry)</term>
<term>Cellulase (metabolism)</term>
<term>Dose-Response Relationship, Drug (MeSH)</term>
<term>Hydrolysis (MeSH)</term>
<term>Phanerochaete (enzymology)</term>
<term>Sodium Hydroxide (pharmacology)</term>
<term>Sulfuric Acids (pharmacology)</term>
<term>Zea mays (chemistry)</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term>
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<front><div type="abstract" xml:lang="en">Two commonly used chemical pretreatment processes, sulphuric acid, and sodium hydroxide, were tested to provide comparative performance data. A connection between solid to liquid ratio (S/L) and sugars released was observed with an increase in S/L ratio between 0.02 and 0.2. Enzymatic digestibility of 1 M of NaOH-pretreated corncobs were released 210.7 mg ml(-1) of sugars. Further, compared with different concentrations of acid pretreatments at 0.05, 0.1, 0.15, 0.2, 0.25, 0.3, and 0.5 M concentrations, sodium hydroxide pretreatment of corncob substantially increased accessibility and digestibility of cellulose. Another additional observation made was whole-cell and crude enzymatic hydrolysis of different concentrations of acid and NaOH (0.05, 0.1, 0.25 M)-treated materials released lower amount of sugars compared with the sugars released (310.9 mg ml(-1)) with whole-cell hydrolysis of 1 M of NaOH-treated corncobs. NaOH-pretreated corncobs contained higher content of sugars and which is more suitable for production of reducing sugars.</div>
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<Abstract><AbstractText>Two commonly used chemical pretreatment processes, sulphuric acid, and sodium hydroxide, were tested to provide comparative performance data. A connection between solid to liquid ratio (S/L) and sugars released was observed with an increase in S/L ratio between 0.02 and 0.2. Enzymatic digestibility of 1 M of NaOH-pretreated corncobs were released 210.7 mg ml(-1) of sugars. Further, compared with different concentrations of acid pretreatments at 0.05, 0.1, 0.15, 0.2, 0.25, 0.3, and 0.5 M concentrations, sodium hydroxide pretreatment of corncob substantially increased accessibility and digestibility of cellulose. Another additional observation made was whole-cell and crude enzymatic hydrolysis of different concentrations of acid and NaOH (0.05, 0.1, 0.25 M)-treated materials released lower amount of sugars compared with the sugars released (310.9 mg ml(-1)) with whole-cell hydrolysis of 1 M of NaOH-treated corncobs. NaOH-pretreated corncobs contained higher content of sugars and which is more suitable for production of reducing sugars.</AbstractText>
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