Aqueous two-phase system purification for superoxide dismutase induced by menadione from Phanerochaete chrysosporium.
Identifieur interne : 000217 ( Main/Corpus ); précédent : 000216; suivant : 000218Aqueous two-phase system purification for superoxide dismutase induced by menadione from Phanerochaete chrysosporium.
Auteurs : Berna Kavakc O Lu ; Burcu Tongul ; Leman TarhanSource :
- Artificial cells, nanomedicine, and biotechnology [ 2169-141X ] ; 2017.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Fungal Proteins, Polyethylene Glycols, Polymers, Propylene Glycols, Superoxide Dismutase.
- chemical , isolation & purification : Fungal Proteins, Superoxide Dismutase.
- enzymology : Phanerochaete.
- chemical , pharmacology : Vitamin K 3.
Abstract
In the present work, the partitioning behavior of menadione-induced superoxide dismutase (SOD; EC 1.15.1.1), an antioxidant enzyme that has various applications in the medical and cosmetic industries, from the white rot fungus Phanerochaete chrysosporium has been characterized on different types of aqueous two-phase systems (ATPSs) (poly(ethylene glycol)/polypropylene glycol (PEG/PPG)-dextran, PEG-salt and PPG-salt). PEG-salt combinations were found most optimal systems for the purification of SOD. The best partition conditions were found using the PEG-3350 24% and K2HPO4 5% (w/w) with pH 7.0 at 25 °C. The partition coefficient of total SOD activity and total protein concentration observed in this system were 0.17 and 6.65, respectively, with the recovery percentage as 78.90% in the bottom phase and 13.17% in the top phase. The highest purification fold for SOD from P. chrysosporium was found as 6.04 in the bottom phase of PEG 3350%24 - K2HPO4%5 (w/w) system with pH 7.0. SOD purified from P. chrysosporium was determined to be a homodimer in its native state with a molecular weight of 60 ± 4 kDa. Consequently, simple and only one step PEG-salt ATPS system was developed for SOD purification from P. chrysosporium.
DOI: 10.3109/21691401.2016.1160404
PubMed: 27022668
Links to Exploration step
pubmed:27022668Le document en format XML
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<author><name sortKey="Kavakc O Lu, Berna" sort="Kavakc O Lu, Berna" uniqKey="Kavakc O Lu B" first="Berna" last="Kavakc O Lu">Berna Kavakc O Lu</name>
<affiliation><nlm:affiliation>a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir , Turkey.</nlm:affiliation>
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<author><name sortKey="Tongul, Burcu" sort="Tongul, Burcu" uniqKey="Tongul B" first="Burcu" last="Tongul">Burcu Tongul</name>
<affiliation><nlm:affiliation>a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir , Turkey.</nlm:affiliation>
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<author><name sortKey="Tarhan, Leman" sort="Tarhan, Leman" uniqKey="Tarhan L" first="Leman" last="Tarhan">Leman Tarhan</name>
<affiliation><nlm:affiliation>a Department of Chemistry, Faculty of Science , University of Dokuz Eylul , Buca, Izmir , Turkey.</nlm:affiliation>
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<author><name sortKey="Kavakc O Lu, Berna" sort="Kavakc O Lu, Berna" uniqKey="Kavakc O Lu B" first="Berna" last="Kavakc O Lu">Berna Kavakc O Lu</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Fungal Proteins (chemistry)</term>
<term>Fungal Proteins (isolation & purification)</term>
<term>Phanerochaete (enzymology)</term>
<term>Polyethylene Glycols (chemistry)</term>
<term>Polymers (chemistry)</term>
<term>Propylene Glycols (chemistry)</term>
<term>Superoxide Dismutase (chemistry)</term>
<term>Superoxide Dismutase (isolation & purification)</term>
<term>Vitamin K 3 (pharmacology)</term>
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<term>Superoxide Dismutase</term>
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<term>Superoxide Dismutase</term>
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<front><div type="abstract" xml:lang="en">In the present work, the partitioning behavior of menadione-induced superoxide dismutase (SOD; EC 1.15.1.1), an antioxidant enzyme that has various applications in the medical and cosmetic industries, from the white rot fungus Phanerochaete chrysosporium has been characterized on different types of aqueous two-phase systems (ATPSs) (poly(ethylene glycol)/polypropylene glycol (PEG/PPG)-dextran, PEG-salt and PPG-salt). PEG-salt combinations were found most optimal systems for the purification of SOD. The best partition conditions were found using the PEG-3350 24% and K<sub>2</sub>
HPO<sub>4</sub>
5% (w/w) with pH 7.0 at 25 °C. The partition coefficient of total SOD activity and total protein concentration observed in this system were 0.17 and 6.65, respectively, with the recovery percentage as 78.90% in the bottom phase and 13.17% in the top phase. The highest purification fold for SOD from P. chrysosporium was found as 6.04 in the bottom phase of PEG 3350%24 - K<sub>2</sub>
HPO<sub>4</sub>
%5 (w/w) system with pH 7.0. SOD purified from P. chrysosporium was determined to be a homodimer in its native state with a molecular weight of 60 ± 4 kDa. Consequently, simple and only one step PEG-salt ATPS system was developed for SOD purification from P. chrysosporium.</div>
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<Abstract><AbstractText>In the present work, the partitioning behavior of menadione-induced superoxide dismutase (SOD; EC 1.15.1.1), an antioxidant enzyme that has various applications in the medical and cosmetic industries, from the white rot fungus Phanerochaete chrysosporium has been characterized on different types of aqueous two-phase systems (ATPSs) (poly(ethylene glycol)/polypropylene glycol (PEG/PPG)-dextran, PEG-salt and PPG-salt). PEG-salt combinations were found most optimal systems for the purification of SOD. The best partition conditions were found using the PEG-3350 24% and K<sub>2</sub>
HPO<sub>4</sub>
5% (w/w) with pH 7.0 at 25 °C. The partition coefficient of total SOD activity and total protein concentration observed in this system were 0.17 and 6.65, respectively, with the recovery percentage as 78.90% in the bottom phase and 13.17% in the top phase. The highest purification fold for SOD from P. chrysosporium was found as 6.04 in the bottom phase of PEG 3350%24 - K<sub>2</sub>
HPO<sub>4</sub>
%5 (w/w) system with pH 7.0. SOD purified from P. chrysosporium was determined to be a homodimer in its native state with a molecular weight of 60 ± 4 kDa. Consequently, simple and only one step PEG-salt ATPS system was developed for SOD purification from P. chrysosporium.</AbstractText>
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