Phanerochaete chrysosporium Multienzyme Catabolic System for in Vivo Modification of Synthetic Lignin to Succinic Acid.
Identifieur interne : 000160 ( Main/Corpus ); précédent : 000159; suivant : 000161Phanerochaete chrysosporium Multienzyme Catabolic System for in Vivo Modification of Synthetic Lignin to Succinic Acid.
Auteurs : Chang-Young Hong ; Sun-Hwa Ryu ; Hanseob Jeong ; Sung-Suk Lee ; Myungkil Kim ; In-Gyu ChoiSource :
- ACS chemical biology [ 1554-8937 ] ; 2017.
English descriptors
- KwdEn :
- Ascorbic Acid (chemistry), Free Radicals (MeSH), Lignin (chemistry), Lignin (metabolism), Molecular Weight (MeSH), Multienzyme Complexes (chemistry), Nitrobenzenes (chemistry), Oxidation-Reduction (MeSH), Phanerochaete (enzymology), Phanerochaete (metabolism), Succinic Acid (chemical synthesis), Succinic Acid (chemistry), Succinic Acid (metabolism), Tocopherols (chemistry).
- MESH :
- chemical , chemical synthesis : Succinic Acid.
- chemical , chemistry : Ascorbic Acid, Lignin, Multienzyme Complexes, Nitrobenzenes, Succinic Acid, Tocopherols.
- chemical , metabolism : Lignin, Succinic Acid.
- chemical : Free Radicals.
- enzymology : Phanerochaete.
- metabolism : Phanerochaete.
- Molecular Weight, Oxidation-Reduction.
Abstract
Whole cells of the basidiomycete fungus Phanerochaete chrysosporium (ATCC 20696) were applied to induce the biomodification of lignin in an in vivo system. Our results indicated that P. chrysosporium has a catabolic system that induces characteristic biomodifications of synthetic lignin through a series of redox reactions, leading not only to the degradation of lignin but also to its polymerization. The reducing agents ascorbic acid and α-tocopherol were used to stabilize the free radicals generated from the ligninolytic process. The application of P. chrysosporium in combination with reducing agents produced aromatic compounds and succinic acid as well as degraded lignin polymers. P. chrysosporium selectively catalyzed the conversion of lignin to succinic acid, which has an economic value. A transcriptomic analysis of P. chrysosporium suggested that the bond cleavage of synthetic lignin was caused by numerous enzymes, including extracellular enzymes such as lignin peroxidase and manganese peroxidase, and that the aromatic compounds released were metabolized in both the short-cut and classical tricarboxylic acid cycles of P. chrysosporium. In conclusion, P. chrysosporium is suitable as a biocatalyst for lignin degradation to produce a value-added product.
DOI: 10.1021/acschembio.7b00046
PubMed: 28463479
Links to Exploration step
pubmed:28463479Le document en format XML
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Jeong</name><affiliation><nlm:affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Sung Suk" sort="Lee, Sung Suk" uniqKey="Lee S" first="Sung-Suk" last="Lee">Sung-Suk Lee</name><affiliation><nlm:affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Myungkil" sort="Kim, Myungkil" uniqKey="Kim M" first="Myungkil" last="Kim">Myungkil Kim</name><affiliation><nlm:affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Choi, In Gyu" sort="Choi, In Gyu" uniqKey="Choi I" first="In-Gyu" last="Choi">In-Gyu Choi</name><affiliation><nlm:affiliation>Department of Forest Sciences, Seoul National University , Seoul, Republic of Korea.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul, Republic of Korea.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Institutes of Green Bio Science and Technology, Seoul National University , Pyeongchang, Republic of Korea.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28463479</idno><idno type="pmid">28463479</idno><idno type="doi">10.1021/acschembio.7b00046</idno><idno type="wicri:Area/Main/Corpus">000160</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000160</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Phanerochaete chrysosporium Multienzyme Catabolic System for in Vivo Modification of Synthetic Lignin to Succinic Acid.</title><author><name sortKey="Hong, Chang Young" sort="Hong, Chang Young" uniqKey="Hong C" first="Chang-Young" last="Hong">Chang-Young Hong</name><affiliation><nlm:affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Ryu, Sun Hwa" sort="Ryu, Sun Hwa" uniqKey="Ryu S" first="Sun-Hwa" last="Ryu">Sun-Hwa Ryu</name><affiliation><nlm:affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Jeong, Hanseob" sort="Jeong, Hanseob" uniqKey="Jeong H" first="Hanseob" last="Jeong">Hanseob Jeong</name><affiliation><nlm:affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Lee, Sung Suk" sort="Lee, Sung Suk" uniqKey="Lee S" first="Sung-Suk" last="Lee">Sung-Suk Lee</name><affiliation><nlm:affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Kim, Myungkil" sort="Kim, Myungkil" uniqKey="Kim M" first="Myungkil" last="Kim">Myungkil Kim</name><affiliation><nlm:affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</nlm:affiliation></affiliation></author><author><name sortKey="Choi, In Gyu" sort="Choi, In Gyu" uniqKey="Choi I" first="In-Gyu" last="Choi">In-Gyu Choi</name><affiliation><nlm:affiliation>Department of Forest Sciences, Seoul National University , Seoul, Republic of Korea.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul, Republic of Korea.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Institutes of Green Bio Science and Technology, Seoul National University , Pyeongchang, Republic of Korea.</nlm:affiliation></affiliation></author></analytic><series><title level="j">ACS chemical biology</title><idno type="eISSN">1554-8937</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascorbic Acid (chemistry)</term><term>Free Radicals (MeSH)</term><term>Lignin (chemistry)</term><term>Lignin (metabolism)</term><term>Molecular Weight (MeSH)</term><term>Multienzyme Complexes (chemistry)</term><term>Nitrobenzenes (chemistry)</term><term>Oxidation-Reduction (MeSH)</term><term>Phanerochaete (enzymology)</term><term>Phanerochaete (metabolism)</term><term>Succinic Acid (chemical synthesis)</term><term>Succinic Acid (chemistry)</term><term>Succinic Acid (metabolism)</term><term>Tocopherols (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Succinic Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ascorbic Acid</term><term>Lignin</term><term>Multienzyme Complexes</term><term>Nitrobenzenes</term><term>Succinic Acid</term><term>Tocopherols</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term><term>Succinic Acid</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Free Radicals</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Weight</term><term>Oxidation-Reduction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Whole cells of the basidiomycete fungus Phanerochaete chrysosporium (ATCC 20696) were applied to induce the biomodification of lignin in an in vivo system. Our results indicated that P. chrysosporium has a catabolic system that induces characteristic biomodifications of synthetic lignin through a series of redox reactions, leading not only to the degradation of lignin but also to its polymerization. The reducing agents ascorbic acid and α-tocopherol were used to stabilize the free radicals generated from the ligninolytic process. The application of P. chrysosporium in combination with reducing agents produced aromatic compounds and succinic acid as well as degraded lignin polymers. P. chrysosporium selectively catalyzed the conversion of lignin to succinic acid, which has an economic value. A transcriptomic analysis of P. chrysosporium suggested that the bond cleavage of synthetic lignin was caused by numerous enzymes, including extracellular enzymes such as lignin peroxidase and manganese peroxidase, and that the aromatic compounds released were metabolized in both the short-cut and classical tricarboxylic acid cycles of P. chrysosporium. In conclusion, P. chrysosporium is suitable as a biocatalyst for lignin degradation to produce a value-added product.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28463479</PMID><DateCompleted><Year>2018</Year><Month>02</Month><Day>07</Day></DateCompleted><DateRevised><Year>2018</Year><Month>06</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1554-8937</ISSN><JournalIssue CitedMedium="Internet"><Volume>12</Volume><Issue>7</Issue><PubDate><Year>2017</Year><Month>07</Month><Day>21</Day></PubDate></JournalIssue><Title>ACS chemical biology</Title><ISOAbbreviation>ACS Chem Biol</ISOAbbreviation></Journal><ArticleTitle>Phanerochaete chrysosporium Multienzyme Catabolic System for in Vivo Modification of Synthetic Lignin to Succinic Acid.</ArticleTitle><Pagination><MedlinePgn>1749-1759</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acschembio.7b00046</ELocationID><Abstract><AbstractText>Whole cells of the basidiomycete fungus Phanerochaete chrysosporium (ATCC 20696) were applied to induce the biomodification of lignin in an in vivo system. Our results indicated that P. chrysosporium has a catabolic system that induces characteristic biomodifications of synthetic lignin through a series of redox reactions, leading not only to the degradation of lignin but also to its polymerization. The reducing agents ascorbic acid and α-tocopherol were used to stabilize the free radicals generated from the ligninolytic process. The application of P. chrysosporium in combination with reducing agents produced aromatic compounds and succinic acid as well as degraded lignin polymers. P. chrysosporium selectively catalyzed the conversion of lignin to succinic acid, which has an economic value. A transcriptomic analysis of P. chrysosporium suggested that the bond cleavage of synthetic lignin was caused by numerous enzymes, including extracellular enzymes such as lignin peroxidase and manganese peroxidase, and that the aromatic compounds released were metabolized in both the short-cut and classical tricarboxylic acid cycles of P. chrysosporium. In conclusion, P. chrysosporium is suitable as a biocatalyst for lignin degradation to produce a value-added product.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hong</LastName><ForeName>Chang-Young</ForeName><Initials>CY</Initials><Identifier Source="ORCID">0000-0003-2252-2929</Identifier><AffiliationInfo><Affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ryu</LastName><ForeName>Sun-Hwa</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jeong</LastName><ForeName>Hanseob</ForeName><Initials>H</Initials><Identifier Source="ORCID">0000-0001-7822-1581</Identifier><AffiliationInfo><Affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Sung-Suk</ForeName><Initials>SS</Initials><AffiliationInfo><Affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Myungkil</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>In-Gyu</ForeName><Initials>IG</Initials><AffiliationInfo><Affiliation>Department of Forest Sciences, Seoul National University , Seoul, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Research Institute of Agriculture and Life Sciences, Seoul National University , Seoul, Republic of Korea.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institutes of Green Bio Science and Technology, Seoul National University , Pyeongchang, Republic of Korea.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>05</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>ACS Chem Biol</MedlineTA><NlmUniqueID>101282906</NlmUniqueID><ISSNLinking>1554-8929</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005609">Free Radicals</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009097">Multienzyme Complexes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009578">Nitrobenzenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>AB6MNQ6J6L</RegistryNumber><NameOfSubstance UI="D019802">Succinic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>E57JCN6SSY</RegistryNumber><NameOfSubstance UI="C036077">nitrobenzene</NameOfSubstance></Chemical><Chemical><RegistryNumber>PQ6CK8PD0R</RegistryNumber><NameOfSubstance UI="D001205">Ascorbic 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MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009578" MajorTopicYN="N">Nitrobenzenes</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019802" MajorTopicYN="N">Succinic Acid</DescriptorName><QualifierName UI="Q000138" MajorTopicYN="Y">chemical synthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D024505" 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