The Conifer Root and Stem Rot Pathogen (Heterobasidion parviporum): Effectome Analysis and Roles in Interspecific Fungal Interactions.
Identifieur interne : 000036 ( Main/Corpus ); précédent : 000035; suivant : 000037The Conifer Root and Stem Rot Pathogen (Heterobasidion parviporum): Effectome Analysis and Roles in Interspecific Fungal Interactions.
Auteurs : Zilan Wen ; Zhen Zeng ; Fei Ren ; Fred O. AsiegbuSource :
- Microorganisms [ 2076-2607 ] ; 2019.
Abstract
Heterobasidion parviporum Niemelä & Korhonen is an economically important basidiomycete, causing root and stem rot disease of Norway spruce (Picea abies (L.) Karst) in Northern Europe. The H. parviporum genome encodes numerous small secreted proteins, which might be of importance for interacting with mycorrhiza symbionts, endophytes, and other saprotrophs. We hypothesized that small secreted proteins from H. parviporum (HpSSPs) are involved in interspecific fungal interaction. To identify HpSSP-coding genes potentially involved, we screened the H. parviporum effectome and compared their transcriptomic profiles during fungal development and in planta tree infection. We further conducted phylogenetic analysis, and identified a subset of hypothetical proteins with nonpredicted domain or unknown function as HpSSPs candidates for further characterization. The HpSSPs candidates were selected based on high-quality sequence, cysteine residue frequency, protein size, and in planta expression. We subsequently explored their roles during in vitro interaction in paired cultures of H. parviporum with ectomycorrhizal Cortinarius gentilis, endophytic Phialocephala sphaeroides, saprotrophs (Mycena sp., Phlebiopsis gigantea, and Phanerochaete chrysosporium), respectively. The transcriptomic profile revealed that a large proportion of effector candidates was either barely expressed or highly expressed under all growth conditions. In vitro dual-culture test showed that P. sphaeroides and C. gentilis were overgrown by H. parviporum. The barrage zone formation or no physical contact observed in paired cultures with the saprotrophs suggest they had either combative interaction or antibiosis effect with H. parviporum. Several HpSSPs individuals were up- or downregulated during the nonself interactions. The results of HpSSPs gene expression patterns provide additional insights into the diverse roles of SSPs in tree infection and interspecific fungal interactions.
DOI: 10.3390/microorganisms7120658
PubMed: 31817407
PubMed Central: PMC6955712
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Conifer Root and Stem Rot Pathogen (<i>Heterobasidion parviporum</i>): Effectome Analysis and Roles in Interspecific Fungal Interactions.</title><author><name sortKey="Wen, Zilan" sort="Wen, Zilan" uniqKey="Wen Z" first="Zilan" last="Wen">Zilan Wen</name><affiliation><nlm:affiliation>Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, University of Helsinki, 00014 Helsinki, Finland.</nlm:affiliation></affiliation></author><author><name sortKey="Zeng, Zhen" sort="Zeng, Zhen" uniqKey="Zeng Z" first="Zhen" last="Zeng">Zhen Zeng</name><affiliation><nlm:affiliation>Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, University of Helsinki, 00014 Helsinki, Finland.</nlm:affiliation></affiliation></author><author><name sortKey="Ren, Fei" sort="Ren, Fei" uniqKey="Ren F" first="Fei" last="Ren">Fei Ren</name><affiliation><nlm:affiliation>Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, University of Helsinki, 00014 Helsinki, Finland.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, No. 1 Shuiza Road, Beijing 102300, China.</nlm:affiliation></affiliation></author><author><name sortKey="Asiegbu, Fred O" sort="Asiegbu, Fred O" uniqKey="Asiegbu F" first="Fred O" last="Asiegbu">Fred O. Asiegbu</name><affiliation><nlm:affiliation>Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, University of Helsinki, 00014 Helsinki, Finland.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31817407</idno><idno type="pmid">31817407</idno><idno type="doi">10.3390/microorganisms7120658</idno><idno type="pmc">PMC6955712</idno><idno type="wicri:Area/Main/Corpus">000036</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000036</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The Conifer Root and Stem Rot Pathogen (<i>Heterobasidion parviporum</i>): Effectome Analysis and Roles in Interspecific Fungal Interactions.</title><author><name sortKey="Wen, Zilan" sort="Wen, Zilan" uniqKey="Wen Z" first="Zilan" last="Wen">Zilan Wen</name><affiliation><nlm:affiliation>Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, University of Helsinki, 00014 Helsinki, Finland.</nlm:affiliation></affiliation></author><author><name sortKey="Zeng, Zhen" sort="Zeng, Zhen" uniqKey="Zeng Z" first="Zhen" last="Zeng">Zhen Zeng</name><affiliation><nlm:affiliation>Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, University of Helsinki, 00014 Helsinki, Finland.</nlm:affiliation></affiliation></author><author><name sortKey="Ren, Fei" sort="Ren, Fei" uniqKey="Ren F" first="Fei" last="Ren">Fei Ren</name><affiliation><nlm:affiliation>Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, University of Helsinki, 00014 Helsinki, Finland.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, No. 1 Shuiza Road, Beijing 102300, China.</nlm:affiliation></affiliation></author><author><name sortKey="Asiegbu, Fred O" sort="Asiegbu, Fred O" uniqKey="Asiegbu F" first="Fred O" last="Asiegbu">Fred O. Asiegbu</name><affiliation><nlm:affiliation>Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, University of Helsinki, 00014 Helsinki, Finland.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Microorganisms</title><idno type="ISSN">2076-2607</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Heterobasidion parviporum</i> Niemelä & Korhonen is an economically important basidiomycete, causing root and stem rot disease of Norway spruce (<i>Picea abies</i> (L.) Karst) in Northern Europe. The <i>H. parviporum</i> genome encodes numerous small secreted proteins, which might be of importance for interacting with mycorrhiza symbionts, endophytes, and other saprotrophs. We hypothesized that small secreted proteins from <i>H. parviporum</i> (HpSSPs) are involved in interspecific fungal interaction. To identify HpSSP-coding genes potentially involved, we screened the <i>H. parviporum</i> effectome and compared their transcriptomic profiles during fungal development and in planta tree infection. We further conducted phylogenetic analysis, and identified a subset of hypothetical proteins with nonpredicted domain or unknown function as HpSSPs candidates for further characterization. The HpSSPs candidates were selected based on high-quality sequence, cysteine residue frequency, protein size, and in planta expression. We subsequently explored their roles during in vitro interaction in paired cultures of <i>H. parviporum</i> with ectomycorrhizal <i>Cortinarius gentilis</i>, endophytic <i>Phialocephala sphaeroides</i>, saprotrophs (<i>Mycena</i> sp., <i>Phlebiopsis gigantea</i>, and <i>Phanerochaete chrysosporium</i>), respectively. The transcriptomic profile revealed that a large proportion of effector candidates was either barely expressed or highly expressed under all growth conditions. In vitro dual-culture test showed that <i>P. sphaeroides</i> and <i>C. gentilis</i> were overgrown by <i>H. parviporum</i>. The barrage zone formation or no physical contact observed in paired cultures with the saprotrophs suggest they had either combative interaction or antibiosis effect with <i>H. parviporum</i>. Several HpSSPs individuals were up- or downregulated during the nonself interactions. The results of HpSSPs gene expression patterns provide additional insights into the diverse roles of SSPs in tree infection and interspecific fungal interactions.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31817407</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2076-2607</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>12</Issue><PubDate><Year>2019</Year><Month>Dec</Month><Day>05</Day></PubDate></JournalIssue><Title>Microorganisms</Title><ISOAbbreviation>Microorganisms</ISOAbbreviation></Journal><ArticleTitle>The Conifer Root and Stem Rot Pathogen (<i>Heterobasidion parviporum</i>): Effectome Analysis and Roles in Interspecific Fungal Interactions.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E658</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/microorganisms7120658</ELocationID><Abstract><AbstractText><i>Heterobasidion parviporum</i> Niemelä & Korhonen is an economically important basidiomycete, causing root and stem rot disease of Norway spruce (<i>Picea abies</i> (L.) Karst) in Northern Europe. The <i>H. parviporum</i> genome encodes numerous small secreted proteins, which might be of importance for interacting with mycorrhiza symbionts, endophytes, and other saprotrophs. We hypothesized that small secreted proteins from <i>H. parviporum</i> (HpSSPs) are involved in interspecific fungal interaction. To identify HpSSP-coding genes potentially involved, we screened the <i>H. parviporum</i> effectome and compared their transcriptomic profiles during fungal development and in planta tree infection. We further conducted phylogenetic analysis, and identified a subset of hypothetical proteins with nonpredicted domain or unknown function as HpSSPs candidates for further characterization. The HpSSPs candidates were selected based on high-quality sequence, cysteine residue frequency, protein size, and in planta expression. We subsequently explored their roles during in vitro interaction in paired cultures of <i>H. parviporum</i> with ectomycorrhizal <i>Cortinarius gentilis</i>, endophytic <i>Phialocephala sphaeroides</i>, saprotrophs (<i>Mycena</i> sp., <i>Phlebiopsis gigantea</i>, and <i>Phanerochaete chrysosporium</i>), respectively. The transcriptomic profile revealed that a large proportion of effector candidates was either barely expressed or highly expressed under all growth conditions. In vitro dual-culture test showed that <i>P. sphaeroides</i> and <i>C. gentilis</i> were overgrown by <i>H. parviporum</i>. The barrage zone formation or no physical contact observed in paired cultures with the saprotrophs suggest they had either combative interaction or antibiosis effect with <i>H. parviporum</i>. Several HpSSPs individuals were up- or downregulated during the nonself interactions. The results of HpSSPs gene expression patterns provide additional insights into the diverse roles of SSPs in tree infection and interspecific fungal interactions.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wen</LastName><ForeName>Zilan</ForeName><Initials>Z</Initials><Identifier Source="ORCID">0000-0002-9388-8760</Identifier><AffiliationInfo><Affiliation>Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, University of Helsinki, 00014 Helsinki, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Zhen</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, University of Helsinki, 00014 Helsinki, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Fei</ForeName><Initials>F</Initials><Identifier Source="ORCID">0000-0002-8335-2161</Identifier><AffiliationInfo><Affiliation>Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, University of Helsinki, 00014 Helsinki, Finland.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, No. 1 Shuiza Road, Beijing 102300, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Asiegbu</LastName><ForeName>Fred O</ForeName><Initials>FO</Initials><Identifier Source="ORCID">0000-0003-0223-7194</Identifier><AffiliationInfo><Affiliation>Faculty of Agriculture and Forestry, P. O. Box 27, Latokartanonkaari 7, University of Helsinki, 00014 Helsinki, Finland.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>grant number 307580</GrantID><Agency>Academy of Finland</Agency><Country></Country></Grant><Grant><GrantID>CAFYBB2018GB001</GrantID><Agency>Research Funds for the Central Non-profit Research Institution of CAF</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>12</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Microorganisms</MedlineTA><NlmUniqueID>101625893</NlmUniqueID><ISSNLinking>2076-2607</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Heterobasidion parviporum</Keyword><Keyword MajorTopicYN="N">gene expression</Keyword><Keyword MajorTopicYN="N">interspecific fungal interaction</Keyword><Keyword MajorTopicYN="N">small secreted protein</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>11</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>11</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>12</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>12</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>12</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>12</Month><Day>11</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31817407</ArticleId><ArticleId 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